The Experts below are selected from a list of 309 Experts worldwide ranked by ideXlab platform
Badreldin H. Ali - One of the best experts on this subject based on the ideXlab platform.
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Betaine (N,N,N-Trimethylglycine) averts photochemically-induced thrombosis in pial microvessels in vivo and platelet aggregation in vitro
Experimental biology and medicine (Maywood N.J.), 2015Co-Authors: Abderrahim Nemmar, Priya Yuvaraju, Sumaya Beegam, Badreldin H. AliAbstract:Betaine (N,N,N-Trimethylglycine) is an important food component with established health benefits through its homocysteine-lowering effects, and is used to lower total homocysteine concentration in plasma of patients with homocystinuria. It is well established that hyperhomocysteinemia is an established risk factor for cardiovascular disease and stroke. However, the possible protective effect of betaine on coagulation events in vivo and in vitro has thus far not been studied. Betaine was given to mice at oral doses of either 10 mg/kg (n = 6) or 40 mg/kg (n = 6) for seven consecutive days, and control mice (n = 6) received water only. The thrombotic occlusion time in photochemically induced thrombosis in pial arterioles was significantly delayed in mice pretreated with betaine at doses of 10 mg/kg (P
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betaine n n n Trimethylglycine averts photochemically induced thrombosis in pial microvessels in vivo and platelet aggregation in vitro
Experimental Biology and Medicine, 2015Co-Authors: Abderrahim Nemmar, Priya Yuvaraju, Sumaya Beegam, Badreldin H. AliAbstract:Betaine (N,N,N-Trimethylglycine) is an important food component with established health benefits through its homocysteine-lowering effects, and is used to lower total homocysteine concentration in plasma of patients with homocystinuria. It is well established that hyperhomocysteinemia is an established risk factor for cardiovascular disease and stroke. However, the possible protective effect of betaine on coagulation events in vivo and in vitro has thus far not been studied. Betaine was given to mice at oral doses of either 10 mg/kg (n = 6) or 40 mg/kg (n = 6) for seven consecutive days, and control mice (n = 6) received water only. The thrombotic occlusion time in photochemically induced thrombosis in pial arterioles was significantly delayed in mice pretreated with betaine at doses of 10 mg/kg (P < 0.001) and 40 mg/kg (P < 0.01). Similar effects were observed in pial venules with 10 mg/kg (P < 0.05) and 40 mg/kg (P < 0.05) betaine. In vitro, in whole blood samples collected from untreated mice (n = 3–5)...
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stress associated with road transportation in desert sheep and goats and the effect of pretreatment with xylazine or sodium betaine
Research in Veterinary Science, 2006Co-Authors: Badreldin H. Ali, A A Alqarawi, H M MousaAbstract:The present work investigates some clinical, endocrinological, biochemical and haematological variables in desert sheep and goats stressed in the course of individual road transportation, and the influence thereon of pretreatment with an established anti-stressor drug, xylazine HCl, and a test compound, sodium betaine (Trimethylglycine). Road transportation for 2 h resulted in variable and statistically insignificant increases in heart, pulse and respiratory rates in both control and experimental animals. Transportation stress significantly increased the concentrations of plasma cortisol, and glucose, and decreased that of magnesium. The endogenous thiocyanate concentration was unaffected. The stress also insignificantly decreased the haematocrit (PCV), and the number of lymphocytes, and increased the concentration of haemoglobin. Pretreatment of sheep and goats with xylazine at a single dose of 0.01 mg/kg by the intravenous route significantly ameliorated the effects induced by the stressful stimulus. The effects of pretreatment of the two species with sodium betaine (10 mg/kg) produced variable and insignificant effects.
Ming Huang - One of the best experts on this subject based on the ideXlab platform.
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experimental and theoretical enthalpies of formation of glycine based sulfate bisulfate amino acid ionic liquids
Journal of Physical Chemistry B, 2012Co-Authors: Ling He, Lei Zhang, Ming HuangAbstract:The experimental and theoretical enthalpies of formation of several structural-similar glycine-based sulfate/bisulfate amino acid ionic liquids including glycine sulfate (Gly2SO4, 1), glycine bisulfate (GlyHSO4, 2), N,N-dimethylglycine sulfate ([DMGly]2SO4, 3), N,N-dimethylglycine bisulfate ([DMGly]HSO4, 4), N,N-dimethylglycine methyl ester sulfate ([DMGlyC1]2SO4, 5), N,N-dimethylglycine methyl ester bisulfate ([DMGlyC1]HSO4, 6), N,N,N-Trimethylglycine methyl ester sulfate ([TMGlyC1]2SO4, 7), and N,N,N-Trimethylglycine methyl ester bisulfate ([TMGlyC1]HSO4, 8) were studied. Their experimental enthalpies of formation were obtained from the corresponding energies of combustion determined by the bomb calorimetry method. The enthalpies of formation of these amino acid ionic liquids are in the range from −1406 kJ mol–1 to −1128 kJ mol–1. Systematic theoretical study on these amino acid ionic liquids were performed by quantum chemistry calculation using the Gaussian03 suite of programs. The geometric optimizati...
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Experimental and Theoretical Enthalpies of Formation of Glycine-Based Sulfate/Bisulfate Amino Acid Ionic Liquids B
The Journal of Physical Chemistry, 2012Co-Authors: Jing-fang Zhu, Ming Huang, Lei Zhang, He Ling, Tao GuohongAbstract:The experimental and theoretical enthalpies of formation of several structural-similar glycine-based sulfate/bisulfate amino acid ionic liquids including glycine sulfate (Gly₂SO₄, 1), glycine bisulfate (GlyHSO₄, 2), N,N-dimethylglycine sulfate ([DMGly]₂SO₄, 3), N,N-dimethylglycine bisulfate ([DMGly]HSO₄, 4), N,N-dimethylglycine methyl ester sulfate ([DMGlyC₁]₂SO₄, 5), N,N-dimethylglycine methyl ester bisulfate ([DMGlyC₁]HSO₄, 6), N,N,N-Trimethylglycine methyl ester sulfate ([TMGlyC₁]₂SO₄, 7), and N,N,N-Trimethylglycine methyl ester bisulfate ([TMGlyC₁]HSO₄, 8) were studied. Their experimental enthalpies of formation were obtained from the corresponding energies of combustion determined by the bomb calorimetry method. The enthalpies of formation of these amino acid ionic liquids are in the range from −1406 kJ mol–¹ to −1128 kJ mol–¹. Systematic theoretical study on these amino acid ionic liquids were performed by quantum chemistry calculation using the Gaussian03 suite of programs. The geometric optimization and the frequency analyses are carried out using the B3LYP method with the 6-31+G** basis set. Their calculated enthalpies of formation were derived from the single point energies carried out with the HF/6-31+G**, B3LYP/6-31+G**, B3LYP/6-311++G**, and MP2/6-311++G** level of theory, respectively. The relevance of experimental and calculated enthalpies of formation was studied. The calculated enthalpies of formation are in good agreement with their experimental data in less than 3% error.
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Experimental and theoretical enthalpies of formation of glycine-based sulfate/bisulfate amino acid ionic liquids.
The journal of physical chemistry. B, 2011Co-Authors: Jing-fang Zhu, Ming Huang, Lei Zhang, Guo-hong TaoAbstract:The experimental and theoretical enthalpies of formation of several structural-similar glycine-based sulfate/bisulfate amino acid ionic liquids including glycine sulfate (Gly2SO4, 1), glycine bisulfate (GlyHSO4, 2), N,N-dimethylglycine sulfate ([DMGly]2SO4, 3), N,N-dimethylglycine bisulfate ([DMGly]HSO4, 4), N,N-dimethylglycine methyl ester sulfate ([DMGlyC1]2SO4, 5), N,N-dimethylglycine methyl ester bisulfate ([DMGlyC1]HSO4, 6), N,N,N-Trimethylglycine methyl ester sulfate ([TMGlyC1]2SO4, 7), and N,N,N-Trimethylglycine methyl ester bisulfate ([TMGlyC1]HSO4, 8) were studied. Their experimental enthalpies of formation were obtained from the corresponding energies of combustion determined by the bomb calorimetry method. The enthalpies of formation of these amino acid ionic liquids are in the range from −1406 kJ mol–1 to −1128 kJ mol–1. Systematic theoretical study on these amino acid ionic liquids were performed by quantum chemistry calculation using the Gaussian03 suite of programs. The geometric optimizati...
Lei Zhang - One of the best experts on this subject based on the ideXlab platform.
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experimental and theoretical enthalpies of formation of glycine based sulfate bisulfate amino acid ionic liquids
Journal of Physical Chemistry B, 2012Co-Authors: Ling He, Lei Zhang, Ming HuangAbstract:The experimental and theoretical enthalpies of formation of several structural-similar glycine-based sulfate/bisulfate amino acid ionic liquids including glycine sulfate (Gly2SO4, 1), glycine bisulfate (GlyHSO4, 2), N,N-dimethylglycine sulfate ([DMGly]2SO4, 3), N,N-dimethylglycine bisulfate ([DMGly]HSO4, 4), N,N-dimethylglycine methyl ester sulfate ([DMGlyC1]2SO4, 5), N,N-dimethylglycine methyl ester bisulfate ([DMGlyC1]HSO4, 6), N,N,N-Trimethylglycine methyl ester sulfate ([TMGlyC1]2SO4, 7), and N,N,N-Trimethylglycine methyl ester bisulfate ([TMGlyC1]HSO4, 8) were studied. Their experimental enthalpies of formation were obtained from the corresponding energies of combustion determined by the bomb calorimetry method. The enthalpies of formation of these amino acid ionic liquids are in the range from −1406 kJ mol–1 to −1128 kJ mol–1. Systematic theoretical study on these amino acid ionic liquids were performed by quantum chemistry calculation using the Gaussian03 suite of programs. The geometric optimizati...
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Experimental and Theoretical Enthalpies of Formation of Glycine-Based Sulfate/Bisulfate Amino Acid Ionic Liquids B
The Journal of Physical Chemistry, 2012Co-Authors: Jing-fang Zhu, Ming Huang, Lei Zhang, He Ling, Tao GuohongAbstract:The experimental and theoretical enthalpies of formation of several structural-similar glycine-based sulfate/bisulfate amino acid ionic liquids including glycine sulfate (Gly₂SO₄, 1), glycine bisulfate (GlyHSO₄, 2), N,N-dimethylglycine sulfate ([DMGly]₂SO₄, 3), N,N-dimethylglycine bisulfate ([DMGly]HSO₄, 4), N,N-dimethylglycine methyl ester sulfate ([DMGlyC₁]₂SO₄, 5), N,N-dimethylglycine methyl ester bisulfate ([DMGlyC₁]HSO₄, 6), N,N,N-Trimethylglycine methyl ester sulfate ([TMGlyC₁]₂SO₄, 7), and N,N,N-Trimethylglycine methyl ester bisulfate ([TMGlyC₁]HSO₄, 8) were studied. Their experimental enthalpies of formation were obtained from the corresponding energies of combustion determined by the bomb calorimetry method. The enthalpies of formation of these amino acid ionic liquids are in the range from −1406 kJ mol–¹ to −1128 kJ mol–¹. Systematic theoretical study on these amino acid ionic liquids were performed by quantum chemistry calculation using the Gaussian03 suite of programs. The geometric optimization and the frequency analyses are carried out using the B3LYP method with the 6-31+G** basis set. Their calculated enthalpies of formation were derived from the single point energies carried out with the HF/6-31+G**, B3LYP/6-31+G**, B3LYP/6-311++G**, and MP2/6-311++G** level of theory, respectively. The relevance of experimental and calculated enthalpies of formation was studied. The calculated enthalpies of formation are in good agreement with their experimental data in less than 3% error.
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Experimental and theoretical enthalpies of formation of glycine-based sulfate/bisulfate amino acid ionic liquids.
The journal of physical chemistry. B, 2011Co-Authors: Jing-fang Zhu, Ming Huang, Lei Zhang, Guo-hong TaoAbstract:The experimental and theoretical enthalpies of formation of several structural-similar glycine-based sulfate/bisulfate amino acid ionic liquids including glycine sulfate (Gly2SO4, 1), glycine bisulfate (GlyHSO4, 2), N,N-dimethylglycine sulfate ([DMGly]2SO4, 3), N,N-dimethylglycine bisulfate ([DMGly]HSO4, 4), N,N-dimethylglycine methyl ester sulfate ([DMGlyC1]2SO4, 5), N,N-dimethylglycine methyl ester bisulfate ([DMGlyC1]HSO4, 6), N,N,N-Trimethylglycine methyl ester sulfate ([TMGlyC1]2SO4, 7), and N,N,N-Trimethylglycine methyl ester bisulfate ([TMGlyC1]HSO4, 8) were studied. Their experimental enthalpies of formation were obtained from the corresponding energies of combustion determined by the bomb calorimetry method. The enthalpies of formation of these amino acid ionic liquids are in the range from −1406 kJ mol–1 to −1128 kJ mol–1. Systematic theoretical study on these amino acid ionic liquids were performed by quantum chemistry calculation using the Gaussian03 suite of programs. The geometric optimizati...
Jing-fang Zhu - One of the best experts on this subject based on the ideXlab platform.
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Experimental and Theoretical Enthalpies of Formation of Glycine-Based Sulfate/Bisulfate Amino Acid Ionic Liquids B
The Journal of Physical Chemistry, 2012Co-Authors: Jing-fang Zhu, Ming Huang, Lei Zhang, He Ling, Tao GuohongAbstract:The experimental and theoretical enthalpies of formation of several structural-similar glycine-based sulfate/bisulfate amino acid ionic liquids including glycine sulfate (Gly₂SO₄, 1), glycine bisulfate (GlyHSO₄, 2), N,N-dimethylglycine sulfate ([DMGly]₂SO₄, 3), N,N-dimethylglycine bisulfate ([DMGly]HSO₄, 4), N,N-dimethylglycine methyl ester sulfate ([DMGlyC₁]₂SO₄, 5), N,N-dimethylglycine methyl ester bisulfate ([DMGlyC₁]HSO₄, 6), N,N,N-Trimethylglycine methyl ester sulfate ([TMGlyC₁]₂SO₄, 7), and N,N,N-Trimethylglycine methyl ester bisulfate ([TMGlyC₁]HSO₄, 8) were studied. Their experimental enthalpies of formation were obtained from the corresponding energies of combustion determined by the bomb calorimetry method. The enthalpies of formation of these amino acid ionic liquids are in the range from −1406 kJ mol–¹ to −1128 kJ mol–¹. Systematic theoretical study on these amino acid ionic liquids were performed by quantum chemistry calculation using the Gaussian03 suite of programs. The geometric optimization and the frequency analyses are carried out using the B3LYP method with the 6-31+G** basis set. Their calculated enthalpies of formation were derived from the single point energies carried out with the HF/6-31+G**, B3LYP/6-31+G**, B3LYP/6-311++G**, and MP2/6-311++G** level of theory, respectively. The relevance of experimental and calculated enthalpies of formation was studied. The calculated enthalpies of formation are in good agreement with their experimental data in less than 3% error.
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Experimental and theoretical enthalpies of formation of glycine-based sulfate/bisulfate amino acid ionic liquids.
The journal of physical chemistry. B, 2011Co-Authors: Jing-fang Zhu, Ming Huang, Lei Zhang, Guo-hong TaoAbstract:The experimental and theoretical enthalpies of formation of several structural-similar glycine-based sulfate/bisulfate amino acid ionic liquids including glycine sulfate (Gly2SO4, 1), glycine bisulfate (GlyHSO4, 2), N,N-dimethylglycine sulfate ([DMGly]2SO4, 3), N,N-dimethylglycine bisulfate ([DMGly]HSO4, 4), N,N-dimethylglycine methyl ester sulfate ([DMGlyC1]2SO4, 5), N,N-dimethylglycine methyl ester bisulfate ([DMGlyC1]HSO4, 6), N,N,N-Trimethylglycine methyl ester sulfate ([TMGlyC1]2SO4, 7), and N,N,N-Trimethylglycine methyl ester bisulfate ([TMGlyC1]HSO4, 8) were studied. Their experimental enthalpies of formation were obtained from the corresponding energies of combustion determined by the bomb calorimetry method. The enthalpies of formation of these amino acid ionic liquids are in the range from −1406 kJ mol–1 to −1128 kJ mol–1. Systematic theoretical study on these amino acid ionic liquids were performed by quantum chemistry calculation using the Gaussian03 suite of programs. The geometric optimizati...
Janusz Stangret - One of the best experts on this subject based on the ideXlab platform.
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Influence of Osmolytes on Protein and Water Structure: A Step To Understanding the Mechanism of Protein Stabilization
2016Co-Authors: Piotr Bruździak, Aneta Panuszko, Janusz StangretAbstract:Results concerning the thermostability of hen egg white lysozyme in aqueous solutions with stabilizing osmolytes, trimethylamine-N-oxide (TMAO), glycine (Gly), and its N-methyl derivatives, N-methylglycine (NMG), N,N-dimethylglycine (DMG), and N,N,N-Trimethylglycine (betaine, TMG), have been presented. The combination of spectroscopic (IR) and calorimetric (DSC) data allowed us to establish a link between osmolytes’ influence on water structure and their ability to thermally stabilize protein molecule. Structural and energetic characteristics of stabilizing osmolytes’ and lysozyme’s hydration water appear to be very similar. The osmolytes increase lysozyme stabilization in the order bulk water < TMAO < TMG < Gly < DMG < NMG, which is consistent with the order corresponding to the value of the most probable oxygen–oxygen distance of water molecules affected by osmolytes in their surrounding. Obtained results verified the hypothesis concerning the role of water molecules in protein stabilization, explained the osmophobic effect, and finally helped to bring us nearer to the exact mechanism of protein stabilization by osmolytes
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Protein thermal stabilization in aqueous solutions of osmolytes.
Acta Biochimica Polonica, 2015Co-Authors: Piotr Bruździak, Aneta Panuszko, Muriel Jourdan, Janusz StangretAbstract:Proteins' thermal stabilization is a significant problem in various biomedical, biotechnological, and technological applications. We investigated thermal stability of hen egg white lysozyme in aqueous solutions of the following stabilizing osmolytes: Glycine (GLY), N-methylglycine (NMG), N,N-dimethylglycine (DMG), N,N,N-Trimethylglycine (TMG), and trimethyl-N-oxide (TMAO). Results of CD-UV spectroscopic investigation were compared with FTIR hydration studies' results. Selected osmolytes increased lysozyme's thermal stability in the following order: Gly>NMG>TMAO≈DMG>TMG. Theoretical calculations (DFT) showed clearly that osmolytes' amino group protons and water molecules interacting with them played a distinctive role in protein thermal stabilization. The results brought us a step closer to the exact mechanism of protein stabilization by osmolytes.
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Influence of Osmolytes on Protein and Water Structure: A Step To Understanding the Mechanism of Protein Stabilization
The journal of physical chemistry. B, 2013Co-Authors: Piotr Bruździak, Aneta Panuszko, Janusz StangretAbstract:Results concerning the thermostability of hen egg white lysozyme in aqueous solutions with stabilizing osmolytes, trimethylamine-N-oxide (TMAO), glycine (Gly), and its N-methyl derivatives, N-methylglycine (NMG), N,N-dimethylglycine (DMG), and N,N,N-Trimethylglycine (betaine, TMG), have been presented. The combination of spectroscopic (IR) and calorimetric (DSC) data allowed us to establish a link between osmolytes’ influence on water structure and their ability to thermally stabilize protein molecule. Structural and energetic characteristics of stabilizing osmolytes’ and lysozyme’s hydration water appear to be very similar. The osmolytes increase lysozyme stabilization in the order bulk water < TMAO < TMG < Gly < DMG < NMG, which is consistent with the order corresponding to the value of the most probable oxygen–oxygen distance of water molecules affected by osmolytes in their surrounding. Obtained results verified the hypothesis concerning the role of water molecules in protein stabilization, explained ...
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Chemometric Method of Spectra Analysis Leading to Isolation of Lysozyme and CtDNA Spectra Affected by Osmolytes
Applied spectroscopy, 2012Co-Authors: Piotr Bruździak, Paulina W. Rakowska, Janusz StangretAbstract:In this paper we present a chemometric method of analysis leading to isolation of Fourier transform infrared (FT-IR) spectra of biomacromolecules (HEW lysozyme, ctDNA) affected by osmolytes (trimethylamine-N-oxide and N,N,N-Trimethylglycine, respectively) in aqueous solutions. The method is based on the difference spectra method primarily used to characterize the structure of solvent affected by solute. The cyclical usage of factor analysis allows precise information to be obtained on the shape of "affected spectra" of analyzed biomacromolecules. "Affected spectra" of selected biomacromolecules give valuable information on their structure in the presence of the osmolytes in solution, as well as on the level of perturbation in dependence of osmolyte concentration. The method also gives a possibility of insight into the mechanism of interaction in presented types of systems. It can be easily adapted to various chemical and biochemical problems where vibrational or ultraviolet-visible (UV-Vis) spectroscopy is used.
