The Experts below are selected from a list of 186 Experts worldwide ranked by ideXlab platform
Yongki Hong - One of the best experts on this subject based on the ideXlab platform.
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viability assay of coralline algae using Triphenyltetrazolium chloride
Fisheries Science, 2006Co-Authors: Sunmee Park, Seeun Kang, Jaesuk Choi, Jiyoung Cho, Seungje Yoon, Donghyun Ahn, Yongki HongAbstract:Seaweed flora has been disappearing on rockyareas of the ocean. Meanwhile, crustose corallinealgae, which are non-articulated calcareous algae,are growing and covering these rock surfaces withpink- or white-colored crusts. This phenomenon isgenerally called algal whitening. It is now recog-nized as a natural hazard adversely affectingmarine ecosystems and damaging commercialfishing areas.
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Quantitative viability of seaweed tissues assessed with 2,3,5-Triphenyltetrazolium chloride
Journal of Applied Phycology, 1998Co-Authors: Bo-hye Nam, Hyung-joo Jin, Se-kwon Kim, Yongki HongAbstract:A spectrophotometric quantification method was optimized to evaluate its utility in seaweed tissue viability tests using the enzymatic reduction of colorless 2,3,5-Triphenyltetrazolium chloride (TTC) to a colored triphenylformazan (TPF). To allow accurate determination of TPF in the seaweed Porphyra thallus and conchocelis, 0.2 g of tissues are incubated with 4 mL of 0.8% TTC reagent in the dark at 20°C for 1 h under a mineral oil layer. The TPF formed in tissues was extracted for 15 min at 60°C with 2 mL of 0.2 N KOH in 25% ethanol. Then TPF is partitioned away by prompt addition of hexane and vortexing. By this procedure, we have observed nearly complete separation of TPF, and observed good spectrophotometric discrimination between TPF and other hexane-soluble pigments at 545 nm. This procedure has proved applicable to a wide range of seaweed taxa; 1 species of Chlorophyta, 4 species of Phaeophyta and 7 species of Rhodophyta tested.
Ashfaq Shuaib - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of an optimal temperature for brain storage in delayed 2, 3,5-Triphenyltetrazolium chloride staining.
Journal of neuroscience methods, 2000Co-Authors: Shah-naz Hayat Khan, Azaad Baziany, Ali Banigesh, Susan J. Hemmings, Ashfaq ShuaibAbstract:Abstract This paper presents the determination of an optimal temperature for delayed 2,3,5-Triphenyltetrazolium chloride (TTC) staining. Twenty-one rats were subjected to right middle cerebral artery embolic stroke and sacrificed 96 h following ischemia. The brains were harvested and stained immediately after sacrifice or stored for 8 h at 21–23°C or 4°C, respectively. The stained sections were scanned and infarct volume calculated. The quality of staining, distinction of borders between infarcted and non-ischemic tissue and ease of differentiating ischemic tissue in colored and grayscale images was assessed. The present study indicates that results of TTC obtained immediately after animal sacrifice, or delayed TTC staining while storing the brains at room temperature or 4°C are comparable.
Merry C. Nishimura - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of 2,3,5-Triphenyltetrazolium chloride staining to delineate rat brain infarcts.
Stroke, 1991Co-Authors: K Isayama, Lawrence H. Pitts, Merry C. NishimuraAbstract:Accurate and reproducible determination of the size and location of cerebral infarcts is critical for the evaluation of experimental focal cerebral ischemia. The purpose of this study was to compare intracardiac perfusion of 2,3,5-Triphenyltetrazolium chloride with immersion of brain tissue in 2,3,5-Triphenyltetrazolium chloride to delineate brain infarcts in rats.After 6, 24, or 48 hours of ischemia induced by permanent middle cerebral artery occlusion, some rats were perfused with 2,3,5-Triphenyltetrazolium chloride; other rats were given an overdose of barbiturates, after which brain sections were immersed in 2,3,5-Triphenyltetrazolium chloride. Coronal sections were taken 4, 6, and 8 mm from the frontal pole, and infarct areas in perfused and immersed sections were compared; subsequently, the same sections were stained with hematoxylin and eosin.In rats subjected to 24 or 48 hours of occlusion, areas of infarction were clearly defined with both 2,3,5-Triphenyltetrazolium chloride staining techniques, ...
Shah-naz Hayat Khan - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of an optimal temperature for brain storage in delayed 2, 3,5-Triphenyltetrazolium chloride staining.
Journal of neuroscience methods, 2000Co-Authors: Shah-naz Hayat Khan, Azaad Baziany, Ali Banigesh, Susan J. Hemmings, Ashfaq ShuaibAbstract:Abstract This paper presents the determination of an optimal temperature for delayed 2,3,5-Triphenyltetrazolium chloride (TTC) staining. Twenty-one rats were subjected to right middle cerebral artery embolic stroke and sacrificed 96 h following ischemia. The brains were harvested and stained immediately after sacrifice or stored for 8 h at 21–23°C or 4°C, respectively. The stained sections were scanned and infarct volume calculated. The quality of staining, distinction of borders between infarcted and non-ischemic tissue and ease of differentiating ischemic tissue in colored and grayscale images was assessed. The present study indicates that results of TTC obtained immediately after animal sacrifice, or delayed TTC staining while storing the brains at room temperature or 4°C are comparable.
Terutoyo Yoshida - One of the best experts on this subject based on the ideXlab platform.
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application of attenuated lactococcus garvieae strain lacking a virulence associated capsule on its cell surface as a live vaccine in yellowtail seriola quinqueradiata temminck and schlegel
Journal of Applied Ichthyology, 2006Co-Authors: T. Ooyama, Y. Shimahara, R. Nomoto, H. Yasuda, K. Iwata, Toshiaki Itami, A Nakamura, Terutoyo YoshidaAbstract:Summary An attenuated Lactococcus garvieae strain lacking a virulence-associated capsule on its cell surface was evaluated for application as a live vaccine. The attenuated strain (MS93003A) was obtained from the parent strain (MS93003V), which produced a well-developed capsule, by culturing on an agar medium supplemented with 2,3,5-Triphenyltetrazolium chloride. When live cells of L. garvieae (MS93003A) or formalin-killed cells (MS93003A) were used as an injectable vaccine, protection against virulent L. garvieae (MS93003V) was conferred on Seriola quinqueradiata. The MS93003A cells did not recover their virulence even after in vivo passages in fish. MS93003A live cells also conferred long-lasting protective immunity to S. quinqueradiata against virulent L. garvieae infection.
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Application of attenuated Lactococcus garvieae strain lacking a virulence‐associated capsule on its cell surface as a live vaccine in yellowtail Seriola quinqueradiata Temminck and Schlegel
Journal of Applied Ichthyology, 2006Co-Authors: T. Ooyama, Y. Shimahara, R. Nomoto, H. Yasuda, K. Iwata, Nakamura A, Toshiaki Itami, Terutoyo YoshidaAbstract:Summary An attenuated Lactococcus garvieae strain lacking a virulence-associated capsule on its cell surface was evaluated for application as a live vaccine. The attenuated strain (MS93003A) was obtained from the parent strain (MS93003V), which produced a well-developed capsule, by culturing on an agar medium supplemented with 2,3,5-Triphenyltetrazolium chloride. When live cells of L. garvieae (MS93003A) or formalin-killed cells (MS93003A) were used as an injectable vaccine, protection against virulent L. garvieae (MS93003V) was conferred on Seriola quinqueradiata. The MS93003A cells did not recover their virulence even after in vivo passages in fish. MS93003A live cells also conferred long-lasting protective immunity to S. quinqueradiata against virulent L. garvieae infection.
