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Elisabeth Haring - One of the best experts on this subject based on the ideXlab platform.

  • Cryptic speciation in pan-tropical sea urchins: a case study of an edge-of-range population of Tripneustes from the Kermadec Islands.
    Scientific reports, 2017
    Co-Authors: Omri Bronstein, Andreas Kroh, Barbara Tautscher, Libby Liggins, Elisabeth Haring
    Abstract:

    Tripneustes is one of the most abundant and ecologically significant tropical echinoids. Highly valued for its gonads, wild populations of Tripneustes are commercially exploited and cultivated stocks are a prime target for the fisheries and aquaculture industry. Here we examine Tripneustes from the Kermadec Islands, a remote chain of volcanic islands in the southwest Pacific Ocean that mark the boundary of the genus' range, by combining morphological and genetic analyses, using two mitochondrial (COI and the Control Region), and one nuclear (bindin) marker. We show that Kermadec Tripneustes is a new species of Tripneustes. We provide a full description of this species and present an updated phylogeny of the genus. This new species, Tripneustes kermadecensis n. sp., is characterized by having ambulacral primary tubercles occurring on every fourth plate ambitally, flattened test with large peristome, one to two occluded plates for every four ambulacral plates, and complete primary series of interambulacral tubercles from peristome to apex. It appears to have split early from the main Tripneustes stock, predating even the split of the Atlantic Tripneustes lineage. Its distinction from the common T. gratilla and potential vulnerability as an isolated endemic species calls for special attention in terms of conservation.

  • do genes lie mitochondrial capture masks the red sea collector urchin s true identity echinodermata echinoidea Tripneustes
    Molecular Phylogenetics and Evolution, 2016
    Co-Authors: Omri Bronstein, Andreas Kroh, Elisabeth Haring
    Abstract:

    Abstract Novel COI and bindin sequences of the Red Sea collector echinoid Tripneustes gratilla elatensis are used to show that (1) discordance between mitochondrial and nuclear loci exists in this echinoid genus, (2) Tripneustes gratilla as currently defined possibly comprises a complex of cryptic species, and (3) Red Sea Tripneustes form a genetically distinct clade in the bindin tree, which diverged from other Tripneustes clades at least 2–4 million years ago. Morphological reassessment of T. gratilla elatensis shows perfect congruence between identification based on skeletal features and genetic data based on a nuclear marker sequence. Hence the Red Sea Tripneustes subspecies established by Dafni in 1983 is a distinct biological unit. All T. g. elatensis samples analyzed are highly similar to or share mtDNA haplotypes with Philippine T. g. gratilla, as do representatives from other edge-of-range occurrences. This lack of genetic structure in Indo-Pacific Tripneustes is interpreted as a result of wide-spread mitochondrial introgression. New fossil specimens from the Red Sea area confirm the sympatric occurrence of T. g. elatensis and T. g. gratilla in the northern Red Sea during Late Pleistocene, identifying a possible timing for the introgression. In addition, present-day distribution shows a contact zone in the Southern Red Sea (in the Dahlak Archipelago). T. g. elatensis, is yet another example of a Red Sea taxon historically identified as conspecific with its Indo-Pacific relatives, but which turned out to be a morphologically and genetically distinct endemic taxon, suggesting that the level of endemism in the Red Sea may still be underestimated.

  • Do genes lie? Mitochondrial capture masks the Red Sea collector urchin’s true identity (Echinodermata: Echinoidea: Tripneustes)
    Molecular phylogenetics and evolution, 2016
    Co-Authors: Omri Bronstein, Andreas Kroh, Elisabeth Haring
    Abstract:

    Abstract Novel COI and bindin sequences of the Red Sea collector echinoid Tripneustes gratilla elatensis are used to show that (1) discordance between mitochondrial and nuclear loci exists in this echinoid genus, (2) Tripneustes gratilla as currently defined possibly comprises a complex of cryptic species, and (3) Red Sea Tripneustes form a genetically distinct clade in the bindin tree, which diverged from other Tripneustes clades at least 2–4 million years ago. Morphological reassessment of T. gratilla elatensis shows perfect congruence between identification based on skeletal features and genetic data based on a nuclear marker sequence. Hence the Red Sea Tripneustes subspecies established by Dafni in 1983 is a distinct biological unit. All T. g. elatensis samples analyzed are highly similar to or share mtDNA haplotypes with Philippine T. g. gratilla, as do representatives from other edge-of-range occurrences. This lack of genetic structure in Indo-Pacific Tripneustes is interpreted as a result of wide-spread mitochondrial introgression. New fossil specimens from the Red Sea area confirm the sympatric occurrence of T. g. elatensis and T. g. gratilla in the northern Red Sea during Late Pleistocene, identifying a possible timing for the introgression. In addition, present-day distribution shows a contact zone in the Southern Red Sea (in the Dahlak Archipelago). T. g. elatensis, is yet another example of a Red Sea taxon historically identified as conspecific with its Indo-Pacific relatives, but which turned out to be a morphologically and genetically distinct endemic taxon, suggesting that the level of endemism in the Red Sea may still be underestimated.

Omri Bronstein - One of the best experts on this subject based on the ideXlab platform.

  • Implications of range overlap in the commercially important pan-tropical sea urchin genus Tripneustes (Echinoidea: Toxopneustidae)
    Marine Biology, 2019
    Co-Authors: Omri Bronstein, Andreas Kroh, Ashley D. Miskelly, Stephen D. A. Smith, Symon A. Dworjanyn, Benjamin Mos, Maria Byrne
    Abstract:

    Sea urchins of the genus Tripneustes are among the most abundant and ecologically important pan-tropical marine invertebrates. Recognized as potent ecosystem engineers due to their intense grazing of macroalgae and sea grass and highly valued for their gonads, wild populations of Tripneustes are commercially exploited for fisheries and aquaculture. Recently, a new species, Tripneustes kermadecensis (Bronstein et al. 2017), was described from the southern Pacific Ocean, off the Kermadec Islands, near the tropical/sub-tropical transition zone. Here, we explore the range of Tripneustes and, in particular, T. kermadecensis by morphological and genetic tools to determine whether it also occurs in Australia. We report, for the first time, the presence of a second Tripneustes species, T. kermadecensis, from Australia. We show that T. kermadecensis is in fact highly abundant throughout most of sub-tropical eastern Australia, where it occurs in association with coral and temperate reefs and has been recognized for decades as the ‘lamington sea urchin’. As commercial exploitation and stock-release programs of Tripneustes are rapidly expanding, and as global warming causes tropicalization of eastern Australia, driving the southern expansion of its congener T. gratilla, we call for re-evaluation of the conservation vulnerability of T. kermadecensis along the Australian continent and action by the aquaculture industry to genetically confirm the species identity of stocks in their facilities.

  • Cryptic speciation in pan-tropical sea urchins: a case study of an edge-of-range population of Tripneustes from the Kermadec Islands.
    Scientific reports, 2017
    Co-Authors: Omri Bronstein, Andreas Kroh, Barbara Tautscher, Libby Liggins, Elisabeth Haring
    Abstract:

    Tripneustes is one of the most abundant and ecologically significant tropical echinoids. Highly valued for its gonads, wild populations of Tripneustes are commercially exploited and cultivated stocks are a prime target for the fisheries and aquaculture industry. Here we examine Tripneustes from the Kermadec Islands, a remote chain of volcanic islands in the southwest Pacific Ocean that mark the boundary of the genus' range, by combining morphological and genetic analyses, using two mitochondrial (COI and the Control Region), and one nuclear (bindin) marker. We show that Kermadec Tripneustes is a new species of Tripneustes. We provide a full description of this species and present an updated phylogeny of the genus. This new species, Tripneustes kermadecensis n. sp., is characterized by having ambulacral primary tubercles occurring on every fourth plate ambitally, flattened test with large peristome, one to two occluded plates for every four ambulacral plates, and complete primary series of interambulacral tubercles from peristome to apex. It appears to have split early from the main Tripneustes stock, predating even the split of the Atlantic Tripneustes lineage. Its distinction from the common T. gratilla and potential vulnerability as an isolated endemic species calls for special attention in terms of conservation.

  • do genes lie mitochondrial capture masks the red sea collector urchin s true identity echinodermata echinoidea Tripneustes
    Molecular Phylogenetics and Evolution, 2016
    Co-Authors: Omri Bronstein, Andreas Kroh, Elisabeth Haring
    Abstract:

    Abstract Novel COI and bindin sequences of the Red Sea collector echinoid Tripneustes gratilla elatensis are used to show that (1) discordance between mitochondrial and nuclear loci exists in this echinoid genus, (2) Tripneustes gratilla as currently defined possibly comprises a complex of cryptic species, and (3) Red Sea Tripneustes form a genetically distinct clade in the bindin tree, which diverged from other Tripneustes clades at least 2–4 million years ago. Morphological reassessment of T. gratilla elatensis shows perfect congruence between identification based on skeletal features and genetic data based on a nuclear marker sequence. Hence the Red Sea Tripneustes subspecies established by Dafni in 1983 is a distinct biological unit. All T. g. elatensis samples analyzed are highly similar to or share mtDNA haplotypes with Philippine T. g. gratilla, as do representatives from other edge-of-range occurrences. This lack of genetic structure in Indo-Pacific Tripneustes is interpreted as a result of wide-spread mitochondrial introgression. New fossil specimens from the Red Sea area confirm the sympatric occurrence of T. g. elatensis and T. g. gratilla in the northern Red Sea during Late Pleistocene, identifying a possible timing for the introgression. In addition, present-day distribution shows a contact zone in the Southern Red Sea (in the Dahlak Archipelago). T. g. elatensis, is yet another example of a Red Sea taxon historically identified as conspecific with its Indo-Pacific relatives, but which turned out to be a morphologically and genetically distinct endemic taxon, suggesting that the level of endemism in the Red Sea may still be underestimated.

  • Do genes lie? Mitochondrial capture masks the Red Sea collector urchin’s true identity (Echinodermata: Echinoidea: Tripneustes)
    Molecular phylogenetics and evolution, 2016
    Co-Authors: Omri Bronstein, Andreas Kroh, Elisabeth Haring
    Abstract:

    Abstract Novel COI and bindin sequences of the Red Sea collector echinoid Tripneustes gratilla elatensis are used to show that (1) discordance between mitochondrial and nuclear loci exists in this echinoid genus, (2) Tripneustes gratilla as currently defined possibly comprises a complex of cryptic species, and (3) Red Sea Tripneustes form a genetically distinct clade in the bindin tree, which diverged from other Tripneustes clades at least 2–4 million years ago. Morphological reassessment of T. gratilla elatensis shows perfect congruence between identification based on skeletal features and genetic data based on a nuclear marker sequence. Hence the Red Sea Tripneustes subspecies established by Dafni in 1983 is a distinct biological unit. All T. g. elatensis samples analyzed are highly similar to or share mtDNA haplotypes with Philippine T. g. gratilla, as do representatives from other edge-of-range occurrences. This lack of genetic structure in Indo-Pacific Tripneustes is interpreted as a result of wide-spread mitochondrial introgression. New fossil specimens from the Red Sea area confirm the sympatric occurrence of T. g. elatensis and T. g. gratilla in the northern Red Sea during Late Pleistocene, identifying a possible timing for the introgression. In addition, present-day distribution shows a contact zone in the Southern Red Sea (in the Dahlak Archipelago). T. g. elatensis, is yet another example of a Red Sea taxon historically identified as conspecific with its Indo-Pacific relatives, but which turned out to be a morphologically and genetically distinct endemic taxon, suggesting that the level of endemism in the Red Sea may still be underestimated.

Magdalena Litaay - One of the best experts on this subject based on the ideXlab platform.

  • The Potency of ??-Sitosterol Isolated From Hydroid Aglaophenia cupressina Lamoureoux as Antimitotic Agent towards Seatowards Sea Urchin Tripneustes gratilla Linn.
    2017
    Co-Authors: Eva Johannes, Magdalena Litaay
    Abstract:

    The potency of ??-Sitosterol isolated from hydroid Aglaophenia cupressina Lamoureoux as\ud antimitotic agent towards early cleavage of Sea Urchin Tripneustes gratilla Linn had been\ud conducted. The study aimed to determine ??-Sitosterol activity isolated compound from hydroid A. cupressina Lamoureoux as antimitotic compound towards early cleavage of zygote of Sea Urchin T. gratilla Linn. Antimitotic treatments were ??-Sitosterol (concentration 1 ??g/ml, 10 ??g/ml and100??g/ml), positive control vincristine (concentration 0.01 ??g/ml, 0.1 ??g/ml, and 1 ??g/ml) and negative control. A protozoa-free seawater was used in this assay. The results revealed that ??-Sitosterol compound had the highest inhibition activity at concentration of 100 ??g/ml was 69.22 % with IC-50 on 0.95 ??g/ml which could develop as cancer chemotherapy compound. Keywords: Antimitotic, ??-Sitosterol, Hydroid Aglaophenia cupressina Lamoureoux, Tripneustes gratilla\ud Linn

  • the potency of β sitosterol isolated from hydroid aglaophenia cupressina lamoureoux as antimitotic agent towards sea urchin Tripneustes gratilla linn
    International Journal of Engineering, 2017
    Co-Authors: Afriansyah Afriansyah, Eva Johannes, Sjafaraenan Sjafaraenan, Magdalena Litaay
    Abstract:

    The potency of β-Sitosterol isolated from hydroid Aglaophenia cupressina Lamoureoux as antimitotic agent towards early cleavage of Sea Urchin Tripneustes gratilla Linn had been conducted. The study aimed to determine β-Sitosterol activity isolated compound from hydroid A. cupressina Lamoureoux as antimitotic compound towards early cleavage of zygote of Sea Urchin T. gratilla Linn. Antimitotic treatments were β-Sitosterol (concentration 1 µg/ml, 10 µg/ml and100 µg/ml), positive control vincristine (concentration 0.01 µg/ml, 0.1 µg/ml, and 1 µg/ml) and negative control.  A protozoa-free seawater was used in this assay. The results revealed that β-Sitosterol compound had the highest inhibition activity at concentration of 100 μg/ml was 69.22 % with IC-50 on 0.95 µg/ml which could develop as cancer chemotherapy compound.

  • UJI ANTIMITOTIK SENYAWA ??-SITOSTEROL HASIL ISOLASI DARI HYDROID Aglaophenia cupressina Lamoureoux TERHADAP PEMBELAHAN AWAL SEL ZIGOT BULU BABI Tripneustes gratilla Linn
    2016
    Co-Authors: Eva Johannes, Magdalena Litaay
    Abstract:

    Telah dilakukan penelitian uji antimitotik senyawa ??-Sitosterol hasil isolasi dari Hydroid Aglaophenia cupressina Lamoureoux terhadap pembelahan awal sel zigot bulu babi Tripneustes gratilla Linn. Penelitian ini bertujuan untuk mengetahui aktivitas senyawa ??-sitosterol hasil isolasi dari hydroid A. cupressina Lamoureoux sebagai bahan antimitotik pembelahan awal (cleavage pertama) sel zigot bulu babi T. gratilla Linn. Uji antimitotik dilakukan terhadap pembelahan awal (cleavage pertama) sel zigot bulu babi T. gratilla Linn dengan senyawa uji ??-Sitosterol (konsentrasi 1 ??g/ml, 10 ??g/ml dan 100 ??g/ml), kontrol positif vinkristin (konsentrasi 0.01 ??g/ml, 0.1 ??g/ml, dan 1 ??g/ml) dan kontrol negatif dengan air laut bebas protozoa (ALBP). Hasil yang diperoleh menunjukkan bahwa senyawa ??-Sitosterol memiliki nilai penghambatan tertinggi pada konsentrasi 100 ??g/ml yaitu 69,22 % dengan nilai IC-50 sebesar 0,95 ??g/ml yang dapat dikembangkan sebagai bahan dasar kemoterapi kanker.\ud \ud Kata Kunci: Antimitotik, ??-Sitosterol, Hydroid Aglaophenia cupressina Lamoureoux, Tripneustes gratilla Linn

Andreas Kroh - One of the best experts on this subject based on the ideXlab platform.

  • Implications of range overlap in the commercially important pan-tropical sea urchin genus Tripneustes (Echinoidea: Toxopneustidae)
    Marine Biology, 2019
    Co-Authors: Omri Bronstein, Andreas Kroh, Ashley D. Miskelly, Stephen D. A. Smith, Symon A. Dworjanyn, Benjamin Mos, Maria Byrne
    Abstract:

    Sea urchins of the genus Tripneustes are among the most abundant and ecologically important pan-tropical marine invertebrates. Recognized as potent ecosystem engineers due to their intense grazing of macroalgae and sea grass and highly valued for their gonads, wild populations of Tripneustes are commercially exploited for fisheries and aquaculture. Recently, a new species, Tripneustes kermadecensis (Bronstein et al. 2017), was described from the southern Pacific Ocean, off the Kermadec Islands, near the tropical/sub-tropical transition zone. Here, we explore the range of Tripneustes and, in particular, T. kermadecensis by morphological and genetic tools to determine whether it also occurs in Australia. We report, for the first time, the presence of a second Tripneustes species, T. kermadecensis, from Australia. We show that T. kermadecensis is in fact highly abundant throughout most of sub-tropical eastern Australia, where it occurs in association with coral and temperate reefs and has been recognized for decades as the ‘lamington sea urchin’. As commercial exploitation and stock-release programs of Tripneustes are rapidly expanding, and as global warming causes tropicalization of eastern Australia, driving the southern expansion of its congener T. gratilla, we call for re-evaluation of the conservation vulnerability of T. kermadecensis along the Australian continent and action by the aquaculture industry to genetically confirm the species identity of stocks in their facilities.

  • Cryptic speciation in pan-tropical sea urchins: a case study of an edge-of-range population of Tripneustes from the Kermadec Islands.
    Scientific reports, 2017
    Co-Authors: Omri Bronstein, Andreas Kroh, Barbara Tautscher, Libby Liggins, Elisabeth Haring
    Abstract:

    Tripneustes is one of the most abundant and ecologically significant tropical echinoids. Highly valued for its gonads, wild populations of Tripneustes are commercially exploited and cultivated stocks are a prime target for the fisheries and aquaculture industry. Here we examine Tripneustes from the Kermadec Islands, a remote chain of volcanic islands in the southwest Pacific Ocean that mark the boundary of the genus' range, by combining morphological and genetic analyses, using two mitochondrial (COI and the Control Region), and one nuclear (bindin) marker. We show that Kermadec Tripneustes is a new species of Tripneustes. We provide a full description of this species and present an updated phylogeny of the genus. This new species, Tripneustes kermadecensis n. sp., is characterized by having ambulacral primary tubercles occurring on every fourth plate ambitally, flattened test with large peristome, one to two occluded plates for every four ambulacral plates, and complete primary series of interambulacral tubercles from peristome to apex. It appears to have split early from the main Tripneustes stock, predating even the split of the Atlantic Tripneustes lineage. Its distinction from the common T. gratilla and potential vulnerability as an isolated endemic species calls for special attention in terms of conservation.

  • do genes lie mitochondrial capture masks the red sea collector urchin s true identity echinodermata echinoidea Tripneustes
    Molecular Phylogenetics and Evolution, 2016
    Co-Authors: Omri Bronstein, Andreas Kroh, Elisabeth Haring
    Abstract:

    Abstract Novel COI and bindin sequences of the Red Sea collector echinoid Tripneustes gratilla elatensis are used to show that (1) discordance between mitochondrial and nuclear loci exists in this echinoid genus, (2) Tripneustes gratilla as currently defined possibly comprises a complex of cryptic species, and (3) Red Sea Tripneustes form a genetically distinct clade in the bindin tree, which diverged from other Tripneustes clades at least 2–4 million years ago. Morphological reassessment of T. gratilla elatensis shows perfect congruence between identification based on skeletal features and genetic data based on a nuclear marker sequence. Hence the Red Sea Tripneustes subspecies established by Dafni in 1983 is a distinct biological unit. All T. g. elatensis samples analyzed are highly similar to or share mtDNA haplotypes with Philippine T. g. gratilla, as do representatives from other edge-of-range occurrences. This lack of genetic structure in Indo-Pacific Tripneustes is interpreted as a result of wide-spread mitochondrial introgression. New fossil specimens from the Red Sea area confirm the sympatric occurrence of T. g. elatensis and T. g. gratilla in the northern Red Sea during Late Pleistocene, identifying a possible timing for the introgression. In addition, present-day distribution shows a contact zone in the Southern Red Sea (in the Dahlak Archipelago). T. g. elatensis, is yet another example of a Red Sea taxon historically identified as conspecific with its Indo-Pacific relatives, but which turned out to be a morphologically and genetically distinct endemic taxon, suggesting that the level of endemism in the Red Sea may still be underestimated.

  • Do genes lie? Mitochondrial capture masks the Red Sea collector urchin’s true identity (Echinodermata: Echinoidea: Tripneustes)
    Molecular phylogenetics and evolution, 2016
    Co-Authors: Omri Bronstein, Andreas Kroh, Elisabeth Haring
    Abstract:

    Abstract Novel COI and bindin sequences of the Red Sea collector echinoid Tripneustes gratilla elatensis are used to show that (1) discordance between mitochondrial and nuclear loci exists in this echinoid genus, (2) Tripneustes gratilla as currently defined possibly comprises a complex of cryptic species, and (3) Red Sea Tripneustes form a genetically distinct clade in the bindin tree, which diverged from other Tripneustes clades at least 2–4 million years ago. Morphological reassessment of T. gratilla elatensis shows perfect congruence between identification based on skeletal features and genetic data based on a nuclear marker sequence. Hence the Red Sea Tripneustes subspecies established by Dafni in 1983 is a distinct biological unit. All T. g. elatensis samples analyzed are highly similar to or share mtDNA haplotypes with Philippine T. g. gratilla, as do representatives from other edge-of-range occurrences. This lack of genetic structure in Indo-Pacific Tripneustes is interpreted as a result of wide-spread mitochondrial introgression. New fossil specimens from the Red Sea area confirm the sympatric occurrence of T. g. elatensis and T. g. gratilla in the northern Red Sea during Late Pleistocene, identifying a possible timing for the introgression. In addition, present-day distribution shows a contact zone in the Southern Red Sea (in the Dahlak Archipelago). T. g. elatensis, is yet another example of a Red Sea taxon historically identified as conspecific with its Indo-Pacific relatives, but which turned out to be a morphologically and genetically distinct endemic taxon, suggesting that the level of endemism in the Red Sea may still be underestimated.

Eva Johannes - One of the best experts on this subject based on the ideXlab platform.

  • The Potency of ??-Sitosterol Isolated From Hydroid Aglaophenia cupressina Lamoureoux as Antimitotic Agent towards Seatowards Sea Urchin Tripneustes gratilla Linn.
    2017
    Co-Authors: Eva Johannes, Magdalena Litaay
    Abstract:

    The potency of ??-Sitosterol isolated from hydroid Aglaophenia cupressina Lamoureoux as\ud antimitotic agent towards early cleavage of Sea Urchin Tripneustes gratilla Linn had been\ud conducted. The study aimed to determine ??-Sitosterol activity isolated compound from hydroid A. cupressina Lamoureoux as antimitotic compound towards early cleavage of zygote of Sea Urchin T. gratilla Linn. Antimitotic treatments were ??-Sitosterol (concentration 1 ??g/ml, 10 ??g/ml and100??g/ml), positive control vincristine (concentration 0.01 ??g/ml, 0.1 ??g/ml, and 1 ??g/ml) and negative control. A protozoa-free seawater was used in this assay. The results revealed that ??-Sitosterol compound had the highest inhibition activity at concentration of 100 ??g/ml was 69.22 % with IC-50 on 0.95 ??g/ml which could develop as cancer chemotherapy compound. Keywords: Antimitotic, ??-Sitosterol, Hydroid Aglaophenia cupressina Lamoureoux, Tripneustes gratilla\ud Linn

  • the potency of β sitosterol isolated from hydroid aglaophenia cupressina lamoureoux as antimitotic agent towards sea urchin Tripneustes gratilla linn
    International Journal of Engineering, 2017
    Co-Authors: Afriansyah Afriansyah, Eva Johannes, Sjafaraenan Sjafaraenan, Magdalena Litaay
    Abstract:

    The potency of β-Sitosterol isolated from hydroid Aglaophenia cupressina Lamoureoux as antimitotic agent towards early cleavage of Sea Urchin Tripneustes gratilla Linn had been conducted. The study aimed to determine β-Sitosterol activity isolated compound from hydroid A. cupressina Lamoureoux as antimitotic compound towards early cleavage of zygote of Sea Urchin T. gratilla Linn. Antimitotic treatments were β-Sitosterol (concentration 1 µg/ml, 10 µg/ml and100 µg/ml), positive control vincristine (concentration 0.01 µg/ml, 0.1 µg/ml, and 1 µg/ml) and negative control.  A protozoa-free seawater was used in this assay. The results revealed that β-Sitosterol compound had the highest inhibition activity at concentration of 100 μg/ml was 69.22 % with IC-50 on 0.95 µg/ml which could develop as cancer chemotherapy compound.

  • aktivitas uji antimitotik senyawa asam heksadekanoat isolat dari hydroid aglaophenia cupressina lamoureoux pada cleavage bulu babi Tripneustes gratilla linn
    BIOMA : JURNAL BIOLOGI MAKASSAR, 2017
    Co-Authors: Sjafaraenan Sjafaraenan, Eva Johannes
    Abstract:

    Abstrak Studi tentang aktivitas antimitotik senyawa asam heksadekanoat isolat dari hydroid Aglaopehenia cupressina Lamoureoux pada cleavage bulu babi Tripneustes gratilla Linn. Penelitian ini bertujuan untuk mengetahui pengaruh senyawa bioaktif asam heksadekanoat dari hydroid Aglaopehenia cupressina Lamoureoux pada cleavage bulu babi Tripneustes gratilla Linn. Metode yang digunakan adalah metode uji aktivitas antimitotik senyawa asam heksadekanoat pada cleavage bulu babi Tripneustes gratilla Linn. pada konsentrasi 1, 10, dan 100 µg/ml dengan kontrol positif vinkristin pada konsentrasi 0.01, 0.1, dan 1 µg/ml setelah fertilisasi penghambatan tertinggi terjadi pada konsentrasi 100 µg/ml yaitu 66,66 %. Kemudian setelah dihitung dengan analisis probit, asam heksadekanoat memiliki IC50 sebesar 22,076 µg/ml yang memiliki kesamaan sifat antimitotik dengan vinkristin yang memiliki IC 50 sebesar 0,219 µg/ml. Sehingga asam heksadekanoat isolat dari hydroid Aglaopehenia cupressina Lamoureoux berpotensi untuk dijadikan bahan dasar anti kanker

  • UJI ANTIMITOTIK SENYAWA ??-SITOSTEROL HASIL ISOLASI DARI HYDROID Aglaophenia cupressina Lamoureoux TERHADAP PEMBELAHAN AWAL SEL ZIGOT BULU BABI Tripneustes gratilla Linn
    2016
    Co-Authors: Eva Johannes, Magdalena Litaay
    Abstract:

    Telah dilakukan penelitian uji antimitotik senyawa ??-Sitosterol hasil isolasi dari Hydroid Aglaophenia cupressina Lamoureoux terhadap pembelahan awal sel zigot bulu babi Tripneustes gratilla Linn. Penelitian ini bertujuan untuk mengetahui aktivitas senyawa ??-sitosterol hasil isolasi dari hydroid A. cupressina Lamoureoux sebagai bahan antimitotik pembelahan awal (cleavage pertama) sel zigot bulu babi T. gratilla Linn. Uji antimitotik dilakukan terhadap pembelahan awal (cleavage pertama) sel zigot bulu babi T. gratilla Linn dengan senyawa uji ??-Sitosterol (konsentrasi 1 ??g/ml, 10 ??g/ml dan 100 ??g/ml), kontrol positif vinkristin (konsentrasi 0.01 ??g/ml, 0.1 ??g/ml, dan 1 ??g/ml) dan kontrol negatif dengan air laut bebas protozoa (ALBP). Hasil yang diperoleh menunjukkan bahwa senyawa ??-Sitosterol memiliki nilai penghambatan tertinggi pada konsentrasi 100 ??g/ml yaitu 69,22 % dengan nilai IC-50 sebesar 0,95 ??g/ml yang dapat dikembangkan sebagai bahan dasar kemoterapi kanker.\ud \ud Kata Kunci: Antimitotik, ??-Sitosterol, Hydroid Aglaophenia cupressina Lamoureoux, Tripneustes gratilla Linn

  • Antimitotic Activity Of Β-Sitosterol Isolated From Hydroid Aglaophenia Cupressina Lamoureoux Against Early Division Of Zygotic Cells Of Sea Urchin Tripneustes Gratilla Linn
    2013
    Co-Authors: Eva Johannes, Syafaraenan, Rosana Agus, Ruslan Umar
    Abstract:

    Hydroid Aglaophenia cupressina Lamoureoux is an invertebrate sea animal which contain various bioactive compounds. These bioactive compounds are used as raw materials for antibiotics and anticancer drugs. This research was aimed to find out the ability of β-sitosterol isolated from hydroid in inhibiting the development of zygotic cells of sea urchin (Tripneustes gratilla Linn.). This study was an experimental study with the following treatment steps: isolation of β-sitosterol from hydroid Agalophenia cupressina Lamoureoux by maceration with methanol, partition with n-hexane, fractionation with column chromatography, UV, IR, and NMR. An antimitotic activity test was then performed with the concentration of 0.1µg/mL, 1 µg/mL, and 10 µg/mL injected into male and female gonad sections of sea urchin (Trupneustes gratilla Linn.). The fertilization procedures involved 1 mL of sperm and 5 mL of ovum fertilized in beaker glass containing 60 mL protozoan-free seawater, and then was incubated. The dividing cells were observed after 2 hours of incubation using a camera-equipped microscope. It was observed that the inhibition rate at 0.1 µg/mL was 49.5%, at 1 µg/mL was 71.5%, and at 10 µg/mL was 86.5%. These results indicated that β-sitosterol compound has the ability to inhibit the division of zygotic cells of sea urchin (T. gratilla Linn.), IC50 = 1,033