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A. Bogdan - One of the best experts on this subject based on the ideXlab platform.
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THE FLORISTIC AND STATIONAL CHARACTERISATION OF THE GRASSLAND SUBTYPE AGROSTIS CAPILLARIS + Trisetum flavescens FROM APUSENI MOUNTAINS, ROMANIA
Research Journal of Agricultural Science, 2010Co-Authors: N. Gârda, Ioan Rotar, Florin Păcurar, A. BogdanAbstract:The floristic composition of the semi - natural grasslands from Apuseni Mountains area differ from one station to another, depending on some factors like: the natural ones, the performed management, by the area's social-economic situations. The objective of this paper is to characterise the grassland subtype Agrostis capillaris - Trisetum flavescens which is present in the grasslands from the Gheţari - Poiana Călineasa Plateau in Gârda de Sus commune, Apuseni Mountains. The identification and description of the respective subtype was elaborated after the analysis of 37 floristic relevees performed using the Braun - Blanquet modified method and, then, by applying some statistic indexes. In our study area, this grassland subtype occures at an altitude comprised between 1070 and 1337 m, the most frequent altitude being 1099 m. The floristic composition of this subtype differs rather much depending on the stational conditions. Thus, the species from Poaceae family are present in average by 48,4 %, with a minimum of 23,5 %
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the floristic and stational characterisation of the grassland subtype agrostis capillaris Trisetum flavescens from apuseni mountains romania
Research Journal of Agricultural Science, 2010Co-Authors: N. Gârda, Ioan Rotar, Florin Păcurar, A. BogdanAbstract:The floristic composition of the semi - natural grasslands from Apuseni Mountains area differ from one station to another, depending on some factors like: the natural ones, the performed management, by the area's social-economic situations. The objective of this paper is to characterise the grassland subtype Agrostis capillaris - Trisetum flavescens which is present in the grasslands from the Gheţari - Poiana Călineasa Plateau in Gârda de Sus commune, Apuseni Mountains. The identification and description of the respective subtype was elaborated after the analysis of 37 floristic relevees performed using the Braun - Blanquet modified method and, then, by applying some statistic indexes. In our study area, this grassland subtype occures at an altitude comprised between 1070 and 1337 m, the most frequent altitude being 1099 m. The floristic composition of this subtype differs rather much depending on the stational conditions. Thus, the species from Poaceae family are present in average by 48,4 %, with a minimum of 23,5 %
Ioan Rotar - One of the best experts on this subject based on the ideXlab platform.
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Ecologic and agronomic aspects of Agrostis capillaris L. - Trisetum flavescens (L.) P. Beauv. grassland subtype.
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Agriculture, 2014Co-Authors: Ioan Rotar, Roxana Vidican, Florin Păcurar, Anca Pleşa, Miklós NagyAbstract:Grasslands in Apuseni Mountains are used in a traditional system and several types and subtypes of grasslands were described. Because of the current grassland management and the site conditions appeared certain grassland subtypes which need to be deepen characterized and their biodiversity and agronomic value needs to be maintain. The aim of the study was to characterize Agrostis capillaris L. - Trisetum flavescens (L.) P. Beauv. grassland subtype from both, ecological and agronomic point of view. The research was unfolded within GA¢rda de Sus commune, on GheA£ari - Poiana CAƒlineasa Plateau. For the floristic composition’s study we used the Braun-BlanquA©t method. The phytocenosis Agrostis capillaris L. - Trisetum flavescens (L.) P. Beauv. has been identified in 37 sites. The sites were charactherized from ecologic and agronomic point of view. This grassland subtype can be characterized as heliophytes, microtherm, mesophytes, moderate acidophilous, medium nitrophilous, medium resistant to mowing, medium resistant to stepping and medium resistant to grazing and endures an animal load of 0,81 - 1,00 LSU/ha.
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the influence of mineral and organic long term fertilization upon the floristic composition of festuca rubra l agrostis capillaris l grassland in apuseni mountains romania
Journal of Food Agriculture & Environment, 2012Co-Authors: Florin Păcurar, Ioan Rotar, Roxana Vidican, Anca Bogdan, Laura DaleAbstract:The semi-natural grasslands in Apuseni Mountains are particularly important for the sustainable development of this region. The future development strategies must take into consideration the conservation of grassland plant diversity. In Apuseni Mountains there are numerous caves and a traditional landscape, these forming the area’s wealth. Their joining with agro-tourism and performing a sustainable agriculture would significantly level up the wellbeing of the local population, who are in need of new income sources considering that the wood resources are more and more reduced. In Gârda de Sus there are considerable areas of semi-natural grasslands which lately have been traditionally used. Nowadays, some of them have been abandoned and others irrationally intensified. Therefore, the objective of this paper was to evaluate the effects of mineral and organic fertilization upon the sward in order to recommend certain versions which have minor repercussions upon the plant diversity. The research was carried out in Apuseni Mountains, in an experimental field with two experiences: one with mineral fertilizers (T 1 control, T 2 50N25P25K, T 3 100N50P50K, T 4 150N75P75K) and the other with organic fertilizers (T 1 control, T 2 10 t ha -1 manure, T 3 20 t ha -1 manure, T 4 30 t ha -1 manure). Administrating mineral fertilizers on the Festuca rubra L.- Agrostis capillaris L. grassland induced the installation of Festuca rubra L. and Trisetum flavescens L. at 50N25P25K treatment, whereas 100N50P50K and 150N75P75K treatments induced the installation of Agrostis capillaris L. and Trisetum flavescens L. grassland subtype. The plant diversity decreased as the system was intensified, especially at the treatment with 100N50P50K and 150N75P75K. Administrating 50N25P25K did not induce any significant changes in plant diversity. Giving organic fertilizers caused installation of Festuca rubra L. and Trisetum flavescens L. at the treatment using 10 t ha -1 manure and Trisetum flavescens L. at using 20 and 30 t ha -1 manure. The treatment with 10 t ha -1 manure induced a growth of the plant diversity and by raising the manure quantity did not cause important changes with respect to the species’ number. For future management actions that are meant to maintain the plant diversity in Apuseni Mountains, the organic fertilization with 10 t ha -1 manure quantities annually administrated or once in two years is recommended first of all, and, secondly, the fertilization with 50N25P25K performed annually or once in two years.
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Research Concerning the Structure and Functioning of Low-Input Grassland Systems
Bulletin of the University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 2011Co-Authors: Florin Păcurar, Ioan Rotar, Anca Bogdan, Roxana VidicanAbstract:The organic fertilizers’ application and the traditional use for long periods of time have influenced the rich plant diversity in Apuseni Mountains, Romania. The objective of this study was to identify the effect of manure’s application upon the plant diversity and productivity of Festuca rubra L. –Agrostis capillaris L. grasslands. The experimental field evaluated four variants (V1: control, V2: 10 t ha-1 manure, V3: 20 t ha-1 manure, V4: 30 t ha-1 manure). After ten years, important changes at the sward level were observed. The control’s phytocenosis is naturally represented by Festuca rubra L. - Agrostis capillaris L. grassland type. Then, following the application of organic fertilizers, Trisetum flavescens L. - Agrostis capillaris L. appeared. The large quantities of organic fertilizers produced a considerable limitation on plant diversity. The applied treatments do not produce any important improvement of the grassland types’ agronomic value. Taking account the influence of organic fertilizers upon the DM yield, plant diversity and agronomic value, it is considered that the treatment with 10 t ha -1 manure annually applied produces optimum changes and might be taken into consideration for the low-input systems in Apuseni Mountains
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Influence of the Management on the Phytocoenotic Biodiversity of Some Romanian Representative Grassland Types
Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2011Co-Authors: Vasile Vântu, Costel Samuil, Ioan Rotar, Alexandru Moisuc, Iosif RazecAbstract:The pastoral area of Romania, covering of over 4.8 million ha, has been influenced, over the last decades, by natural and human factors, which affect the normal functioning of the grassland ecosystem. The aim of this paper is to point out the dynamics of phytocoenotic biodiversity, as a result of applying grassland technical measures, in order to improve the yield and quality of grassland ecosystems with minimum effects on the environment. Therefore, the main aim of our study is to establish a positive relationship between biodiversity and the optimum quantity of organic fertilizers, which would improve the quality and quantity of the yield, without diminishing the floristic biodiversity. In the case of the studied grasslands, the phytocenotic biodiversity is influenced by the type of fertilization, the used rates and by the soil and climatic conditions. These grasslands may be improved by manure fertilization and reasonable usage, if proper management is applied. In Gradinari, Caras-Severin county, the dominant species are Festuca rupicola and Calamagrostis epigeios, in Magurele, Brasov county, Agrostis capillaris, Festuca pratensis, Poa pratensis and Trifolium repens, and in Pojorata, Suceava county, Agrostis capillaris, Trisetum flavescens, Trifolium repens and T. pratense species. The highest number of identified species (43) has been recorded by the meadow made up of Agrostis capillaris and Festuca rubra, from Pojorata, Suceava county.
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THE FLORISTIC AND STATIONAL CHARACTERISATION OF THE GRASSLAND SUBTYPE AGROSTIS CAPILLARIS + Trisetum flavescens FROM APUSENI MOUNTAINS, ROMANIA
Research Journal of Agricultural Science, 2010Co-Authors: N. Gârda, Ioan Rotar, Florin Păcurar, A. BogdanAbstract:The floristic composition of the semi - natural grasslands from Apuseni Mountains area differ from one station to another, depending on some factors like: the natural ones, the performed management, by the area's social-economic situations. The objective of this paper is to characterise the grassland subtype Agrostis capillaris - Trisetum flavescens which is present in the grasslands from the Gheţari - Poiana Călineasa Plateau in Gârda de Sus commune, Apuseni Mountains. The identification and description of the respective subtype was elaborated after the analysis of 37 floristic relevees performed using the Braun - Blanquet modified method and, then, by applying some statistic indexes. In our study area, this grassland subtype occures at an altitude comprised between 1070 and 1337 m, the most frequent altitude being 1099 m. The floristic composition of this subtype differs rather much depending on the stational conditions. Thus, the species from Poaceae family are present in average by 48,4 %, with a minimum of 23,5 %
Florin Păcurar - One of the best experts on this subject based on the ideXlab platform.
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Ecologic and agronomic aspects of Agrostis capillaris L. - Trisetum flavescens (L.) P. Beauv. grassland subtype.
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Agriculture, 2014Co-Authors: Ioan Rotar, Roxana Vidican, Florin Păcurar, Anca Pleşa, Miklós NagyAbstract:Grasslands in Apuseni Mountains are used in a traditional system and several types and subtypes of grasslands were described. Because of the current grassland management and the site conditions appeared certain grassland subtypes which need to be deepen characterized and their biodiversity and agronomic value needs to be maintain. The aim of the study was to characterize Agrostis capillaris L. - Trisetum flavescens (L.) P. Beauv. grassland subtype from both, ecological and agronomic point of view. The research was unfolded within GA¢rda de Sus commune, on GheA£ari - Poiana CAƒlineasa Plateau. For the floristic composition’s study we used the Braun-BlanquA©t method. The phytocenosis Agrostis capillaris L. - Trisetum flavescens (L.) P. Beauv. has been identified in 37 sites. The sites were charactherized from ecologic and agronomic point of view. This grassland subtype can be characterized as heliophytes, microtherm, mesophytes, moderate acidophilous, medium nitrophilous, medium resistant to mowing, medium resistant to stepping and medium resistant to grazing and endures an animal load of 0,81 - 1,00 LSU/ha.
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the influence of mineral and organic long term fertilization upon the floristic composition of festuca rubra l agrostis capillaris l grassland in apuseni mountains romania
Journal of Food Agriculture & Environment, 2012Co-Authors: Florin Păcurar, Ioan Rotar, Roxana Vidican, Anca Bogdan, Laura DaleAbstract:The semi-natural grasslands in Apuseni Mountains are particularly important for the sustainable development of this region. The future development strategies must take into consideration the conservation of grassland plant diversity. In Apuseni Mountains there are numerous caves and a traditional landscape, these forming the area’s wealth. Their joining with agro-tourism and performing a sustainable agriculture would significantly level up the wellbeing of the local population, who are in need of new income sources considering that the wood resources are more and more reduced. In Gârda de Sus there are considerable areas of semi-natural grasslands which lately have been traditionally used. Nowadays, some of them have been abandoned and others irrationally intensified. Therefore, the objective of this paper was to evaluate the effects of mineral and organic fertilization upon the sward in order to recommend certain versions which have minor repercussions upon the plant diversity. The research was carried out in Apuseni Mountains, in an experimental field with two experiences: one with mineral fertilizers (T 1 control, T 2 50N25P25K, T 3 100N50P50K, T 4 150N75P75K) and the other with organic fertilizers (T 1 control, T 2 10 t ha -1 manure, T 3 20 t ha -1 manure, T 4 30 t ha -1 manure). Administrating mineral fertilizers on the Festuca rubra L.- Agrostis capillaris L. grassland induced the installation of Festuca rubra L. and Trisetum flavescens L. at 50N25P25K treatment, whereas 100N50P50K and 150N75P75K treatments induced the installation of Agrostis capillaris L. and Trisetum flavescens L. grassland subtype. The plant diversity decreased as the system was intensified, especially at the treatment with 100N50P50K and 150N75P75K. Administrating 50N25P25K did not induce any significant changes in plant diversity. Giving organic fertilizers caused installation of Festuca rubra L. and Trisetum flavescens L. at the treatment using 10 t ha -1 manure and Trisetum flavescens L. at using 20 and 30 t ha -1 manure. The treatment with 10 t ha -1 manure induced a growth of the plant diversity and by raising the manure quantity did not cause important changes with respect to the species’ number. For future management actions that are meant to maintain the plant diversity in Apuseni Mountains, the organic fertilization with 10 t ha -1 manure quantities annually administrated or once in two years is recommended first of all, and, secondly, the fertilization with 50N25P25K performed annually or once in two years.
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Research Concerning the Structure and Functioning of Low-Input Grassland Systems
Bulletin of the University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 2011Co-Authors: Florin Păcurar, Ioan Rotar, Anca Bogdan, Roxana VidicanAbstract:The organic fertilizers’ application and the traditional use for long periods of time have influenced the rich plant diversity in Apuseni Mountains, Romania. The objective of this study was to identify the effect of manure’s application upon the plant diversity and productivity of Festuca rubra L. –Agrostis capillaris L. grasslands. The experimental field evaluated four variants (V1: control, V2: 10 t ha-1 manure, V3: 20 t ha-1 manure, V4: 30 t ha-1 manure). After ten years, important changes at the sward level were observed. The control’s phytocenosis is naturally represented by Festuca rubra L. - Agrostis capillaris L. grassland type. Then, following the application of organic fertilizers, Trisetum flavescens L. - Agrostis capillaris L. appeared. The large quantities of organic fertilizers produced a considerable limitation on plant diversity. The applied treatments do not produce any important improvement of the grassland types’ agronomic value. Taking account the influence of organic fertilizers upon the DM yield, plant diversity and agronomic value, it is considered that the treatment with 10 t ha -1 manure annually applied produces optimum changes and might be taken into consideration for the low-input systems in Apuseni Mountains
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THE FLORISTIC AND STATIONAL CHARACTERISATION OF THE GRASSLAND SUBTYPE AGROSTIS CAPILLARIS + Trisetum flavescens FROM APUSENI MOUNTAINS, ROMANIA
Research Journal of Agricultural Science, 2010Co-Authors: N. Gârda, Ioan Rotar, Florin Păcurar, A. BogdanAbstract:The floristic composition of the semi - natural grasslands from Apuseni Mountains area differ from one station to another, depending on some factors like: the natural ones, the performed management, by the area's social-economic situations. The objective of this paper is to characterise the grassland subtype Agrostis capillaris - Trisetum flavescens which is present in the grasslands from the Gheţari - Poiana Călineasa Plateau in Gârda de Sus commune, Apuseni Mountains. The identification and description of the respective subtype was elaborated after the analysis of 37 floristic relevees performed using the Braun - Blanquet modified method and, then, by applying some statistic indexes. In our study area, this grassland subtype occures at an altitude comprised between 1070 and 1337 m, the most frequent altitude being 1099 m. The floristic composition of this subtype differs rather much depending on the stational conditions. Thus, the species from Poaceae family are present in average by 48,4 %, with a minimum of 23,5 %
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the floristic and stational characterisation of the grassland subtype agrostis capillaris Trisetum flavescens from apuseni mountains romania
Research Journal of Agricultural Science, 2010Co-Authors: N. Gârda, Ioan Rotar, Florin Păcurar, A. BogdanAbstract:The floristic composition of the semi - natural grasslands from Apuseni Mountains area differ from one station to another, depending on some factors like: the natural ones, the performed management, by the area's social-economic situations. The objective of this paper is to characterise the grassland subtype Agrostis capillaris - Trisetum flavescens which is present in the grasslands from the Gheţari - Poiana Călineasa Plateau in Gârda de Sus commune, Apuseni Mountains. The identification and description of the respective subtype was elaborated after the analysis of 37 floristic relevees performed using the Braun - Blanquet modified method and, then, by applying some statistic indexes. In our study area, this grassland subtype occures at an altitude comprised between 1070 and 1337 m, the most frequent altitude being 1099 m. The floristic composition of this subtype differs rather much depending on the stational conditions. Thus, the species from Poaceae family are present in average by 48,4 %, with a minimum of 23,5 %
N. Gârda - One of the best experts on this subject based on the ideXlab platform.
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THE FLORISTIC AND STATIONAL CHARACTERISATION OF THE GRASSLAND SUBTYPE AGROSTIS CAPILLARIS + Trisetum flavescens FROM APUSENI MOUNTAINS, ROMANIA
Research Journal of Agricultural Science, 2010Co-Authors: N. Gârda, Ioan Rotar, Florin Păcurar, A. BogdanAbstract:The floristic composition of the semi - natural grasslands from Apuseni Mountains area differ from one station to another, depending on some factors like: the natural ones, the performed management, by the area's social-economic situations. The objective of this paper is to characterise the grassland subtype Agrostis capillaris - Trisetum flavescens which is present in the grasslands from the Gheţari - Poiana Călineasa Plateau in Gârda de Sus commune, Apuseni Mountains. The identification and description of the respective subtype was elaborated after the analysis of 37 floristic relevees performed using the Braun - Blanquet modified method and, then, by applying some statistic indexes. In our study area, this grassland subtype occures at an altitude comprised between 1070 and 1337 m, the most frequent altitude being 1099 m. The floristic composition of this subtype differs rather much depending on the stational conditions. Thus, the species from Poaceae family are present in average by 48,4 %, with a minimum of 23,5 %
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the floristic and stational characterisation of the grassland subtype agrostis capillaris Trisetum flavescens from apuseni mountains romania
Research Journal of Agricultural Science, 2010Co-Authors: N. Gârda, Ioan Rotar, Florin Păcurar, A. BogdanAbstract:The floristic composition of the semi - natural grasslands from Apuseni Mountains area differ from one station to another, depending on some factors like: the natural ones, the performed management, by the area's social-economic situations. The objective of this paper is to characterise the grassland subtype Agrostis capillaris - Trisetum flavescens which is present in the grasslands from the Gheţari - Poiana Călineasa Plateau in Gârda de Sus commune, Apuseni Mountains. The identification and description of the respective subtype was elaborated after the analysis of 37 floristic relevees performed using the Braun - Blanquet modified method and, then, by applying some statistic indexes. In our study area, this grassland subtype occures at an altitude comprised between 1070 and 1337 m, the most frequent altitude being 1099 m. The floristic composition of this subtype differs rather much depending on the stational conditions. Thus, the species from Poaceae family are present in average by 48,4 %, with a minimum of 23,5 %
Dan‐sook Regal - One of the best experts on this subject based on the ideXlab platform.
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Experimentelle Untersuchungen zur extraossären Verkalkung bei der Hypervitaminose D3
Zentralblatt fur Veterinarmedizin. Reihe A, 2010Co-Authors: Dan‐sook RegalAbstract:Zusammenfassung Im Tierexperiment an Wachteln wurde untersucht, inwieweit die heimische kalzinogene Pflanze Trisetum flavescens (Goldhafer) fur die Entwicklung einer extraossaren Kalzifizierung verantwortlich ist. Es wurden verschiedene Gruppen von Tieren gebildet, die nach einer 17tagigen Depletion mit unterschiedlichen Vitamin D3-Mengen sowie mit lyophilisiertem Goldhafer gefuttert wurden, wahrend eine Kontrollgruppe Standardfutter erhielt. Mittels ultrahistochemischer Methoden wurde die Bildung von Proteoglykanen (PG) sowie loslichem und mineralisiertem Kalzium in Herzmuskel, Niere und Aorta untersucht. Bei den mit der hochsten Vitamin D3-Dosis (4000 i. E./Tag) sowie mit Goldhafer angefutterten Tieren geht eine massive PG-Vermehrung mit einem nahezu gleichzeitigen Maximum an loslichem Ca++ einher, ausgedehnte Kalksalzablagerungen folgen etwa 7 Tage spater an jenen Stellen, die zuvor PG-Ansammlungen aufgewiesen hatten. Bevorzugte Lokalisationen waren die Basalmembranen, die Kapillaren und das interstitielle Bindegewebe von Herzmuskel und Nierenrinde sowie die elastischen Fasern und die Fortsatze der glatten Muskelzellen der Media der Aorta. Die licht- und elektronenoptischen Befunde ergaben eine Vitamin D3-analoge Wirkung von Goldhafer. Dabei kommt es primar zu einer Vermehrung der PG in der Grundsubstanz, in die dann Kalksalze eingelagert werden. Diese Verkalkungen fuhren zu einer sekundaren Degeneration des Gewebes. Summary Experimental studies on extra-osseous calcification in hypervitaminosis D3 II. Effect of Goldhafer (Trisetum flavescens) on formation of proteoglycans in the cardiovascular system and kidneys of quail In experiments on quail the degree to which the native calcinogenic plant Trisetum flavescens is responsible for extraosseous calcification was examined. Different groups of animals were depleted for 17 days and then were given differing amounts of vitamin D3 or of lyophilized Goldhafer, whereas a control group received a standard diet. By means of ultrahistochemical methods the formation of proteoglycans (PG) and of soluble and mineralized Ca was studied in heart muscle, kidney and aorta. With the highest vitamin D3 dose (4000 i.u./day) and with the animals fed Goldhafer there occurred a massive increase in PG and an almost simultaneous maximum of soluble Ca++ and extensive Ca deposits 7 days later in those sites where PG deposits had been found. Favoured sites were basal membranes, capillaries and the interstitial connective tissue of heart muscle and renal cortex, as well as elastic fibres and the processes of smooth muscle cells in the media of the aorta. Light- and electron optical studies showed a vitamin D3-analogue effect of Goldhafer, whereby the primary event was an increase in PG in the ground substance in which then Ca salts were deposited. These foci of calcification led to a secondary degeneration of the tissue. Resume Recherches experimentales sur la calcification extraosseuse lors de l'hypervitaminose D3 II. Influence de l'avoine doree sur la formation de proteoglycanes dans le systeme cardiovasculaire et les reins chez des cailles On a recherche en experimentation animale sur des cailles a quel point la plante indigene calcinogene Trisetum flavescens (avoine doree) etait responsable du developpement d'une calcification extraosseuse. Differents groupes d'animaux ont ete formes et ont recu apres 17 jours de depletion differentes quantites de vitamine D3 et de l'avoine doree lyophilisee alors que des groupes de controle recevaient un aliment standard. On a recherche la formation de proteoglycanes (PG) ainsi que du calcium soluble et mineralise dans le myocarde, les reins et l'aorte au moyen de methodes ultrahistochimiqies. Les animaux ayant recu la plus forte dose de vitamine D3 (4000 i. E./ jour) et de l'avoine doree ont presente une multiplication massive de PG et un maximum en Ca++ soluble a eu apres en meme temps; des depots de sels calcaire etendus ont suivi environ 7 jours plus tard dans les endroits qui presentaient des amas de PG auparavant. Les localisations particulierement touchees furent les membranes basales, les capillaires et le tissu conjonctif interstitiel du myocarde et du cortex renal comme les fibres elastiques et les appendices des cellules de la musculature lisse de la Media de l'aorte. Les resultats du microscope optique comme electronique ont revele un effet analogue a la vitamine D3 de l'avoine doree. On a primairement une multiplication de PG dans la substance fondamentale dans laquelle s'accumulent des sels calcaires. Ces calcifications conduisent a une degenerescence secondaire du tissu. Resumen Estudios experimentales sobre la calcificacion extraosea en la hipervitaminosis D3 II. Influjo de la avena dorada sobre la formacion de proteoglucanas en el sistema cardiovascular y en el rinon de codornices En el experimento animal con codornices se estudio hasta que punto es responsable la planta calcinogena vernacula Trisetum flavescens (avena dorada) del desarrollo de una calcificacion extraosea. Se formaron varios grupos de animales, los cuales fueron alimentados tras una deplecion que duro 17 dias con cantidades diversas de vitamina D3 asi como con avena dorada liofilizada, mientras que un grupo testigo recibio pienso estandar. Mediante tecnicas ultrahistoquimicas se examino la formacion de proteoglucanas (PG) asi como de calcio soluble y mineralizado en miocardio, rinon y aorta. En los animales que recibieron con la comida las dosis maximas de vitamina D3 (4.000 u. i./dia asi como avena dorada, se aprecian una multiplicacion masiva de PG con un maximo casi simultaneo de Ca++ soluble; concreciones prolijas de sales calcicas siguen unos 7 dias mas tarde en aquellos lugares que habian presentado antes acumulaciones de PG. Localizaciones preferidas eran las membranas basales, los capilares y el tejido conjuntivo intersticial del miocardio y corteza renal asi como las fibras elasticas y los apendices de las celulas musculares lisas de la media de la aorta. Los hallazgos foto y electronicoopticos tuvieron por resultado una accion analoga a la vitamina D3 de la avena dorada. Aqui se produjo siempre primariamente una multiplicacion de la PG en la substancia fundamental, en la cual se entreponen entonces las sales calcicas. Estas calcificaciones conducen a una degeneracion secundaria del tejido.
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Experimentelle Untersuchungen zur extraossären Verkalkung bei der Hypervitaminose D3: IV. Licht- und elektronenoptische Untersuchungen zur Lokalisation von intestinalem Kalzium-bindendem Protein (CaBP) bei Wachteln
Zentralblatt fur Veterinarmedizin. Reihe A, 2010Co-Authors: Dan‐sook RegalAbstract:Zusammenfassung Das Vitamin-D-abhangige CaBP wurde im Dunndarm von Wachteln, die nach 17tagiger Depletion wieder mit Vitamin D3 bzw. Goldhafer angefuttert wurden, licht- und elektronenoptisch mittels indirekter Immunofluoreszenz- bzw. Immunoperoxidasetechnik nachgewiesen. Bei der Praparation der Schnitte kamen 3 Verfahren zur Anwendung: A: Gefrier-Auftau, B: Gefriertrocknung, C: Gefriersubstitution. Zwei distinkte Verteilungsmuster von CaBP wurden beobachtet: Methode A ergab eine Lokalisation von CaBP in den Becherzellen, Methode B und C im Zytoplasma der Enterozyten und am Burstensaum. Die ultrastrukturelle Auswertung zeigte CaBP mit Methode A in den Schleimtropfen der Becherzellen, mit Methode B und C in den Mikrovilli sowie in den Ribo- und Polysomen, in den Mitochondrien und im Bereich des terminalen Gespinstes der Enterozyten; die Becherzellen und die subepithelialen Schichten blieben frei von CaBP. Eine Interpretation der drei verwendeten Methoden last den Schlus zu, das das Vorkommen von CaBP in den Becherzellen auf einen methodisch bedingten Artefakt zuruckzufuhren ist, und das die wahre in-situ-Lokalisation des intestinalen CaBPs im Zytoplasma der Enterozyten gelegen ist. Die Untersuchungen haben nicht nur gezeigt, das Goldhafer in der Lage ist, die de-novo-Synthese des Vitamin-D-abhangigen CaBPs bei Vitamin-D3-depletierten Wachteln zu induzieren. Sie geben auch Grund zur Annahme, das CaBP am intrazellularen Kalziumtransport beteiligt ist bzw. das ihm eine Funktion bei der Aufrechterhaltung der intrazellularen Kalzium-Homoostase zukommt. Summary Experimental studies on extraosseous calcification in hypervitaminosis D3 IV. Light and electron-optical studies on localization of intestinal Calcium-binding protein (CaBP) in quails Vitamin D-dependent CaBP in the duodenum of quails which, after 17 days depletion, were repleted with either vitamin D3 or Goldhafer (Trisetum flavescens) was studied by light and electron-optical methods by means of indirect immunofluorescence or immuno-peroxidase techniques. For preparations of sections 3 techniques were used; A. Freezing-thawing, B. Freeze-drying. C. Freeze substitution. Two distinct forms of distribution of CaBP were found. Method A gave a localization of CaBP in goblet cells; methods B and C in the cytoplasm of enterocytes and cilial the border. Ultrastructural evaluation showed CaBP with method A in the mucoid droplets of goblet cells, methods B and C in the microvilli and in ribosomes and polysomes, in the mitochondria and in the region of the terminal web of enterocytes; goblet cells and subepithelial layers remained free of CaBP. An interpretation of the 3 methods allows the conclusion that the occurrence of CaBP in the goblet cells is attributable to a technically induced artefact and that the true in situ localization of CaBP is the cytoplasm of enterocytes. These studies have shown not only that Goldhafer is able to induce de novo synthesis of vitamin D3-dependent CaBP in the vitamin D3-depleted quail but also to give support to the view that CaBP is concerned in intracellular transport of Ca and plays a part in maintaining intracellular Ca homoeostasis. Resume Recherches experimentales sur la calcification extraosseuse lors d'hypervitaminose D3 IV. Recherche au microscope optique et electronique sur la localisation de la proteine intestinale liant le calcium (CaBP) chez des cailles On a mis en evidence au microscope optique et electronique au moyen de l'immunofluorescence indirecte et de la technique de l'immunoperoxydase CaBP dependant de la vitamine D dans l'intestin grele de cailles a nouveau alimentees avec de la vitamine D3 et de l'avoine doree apres une depletion de 17 jours. Trois procedes ont ete utilises pour la preparation des coupes: A: congelation-degel, B: lyophilisation, C: substitution par congelation. Deux sortes distinctes de repartition de CaBP ont ete observees: la methode A a donne une localisation de CaBP dans les cellules caliciformes, la methode B et C dans le cytoplasme des enterocytes et sur la bordure en brosse. L'interpretation ultrastructurelle a montre CaBP dans les gouttes de mucus des cellules caliciformes avec la methode A, dans les microvillosites, dans les ribosomes et les polysomes, dans les mitochondries et la region de la fibre terminale des enterocytes avec les methodes B et C; les cellules caliciformes et les couches sous-epitheliales sont restees libres de CaBP. Une interpretation des trois methodes utilisees a permis de conclure que la presence de CaBP dans les cellules caliciformes etait due a un artefact et que la veritable localisation in situ de CaBP intestinal se situait dans le cytoplasme de enterocytes. Les recherches n'ont pas seulement montre que l'avoine doree est en mesure d'induire la synthese de novo de CaBP dependant de la vitamine D chez des cailles en deepletion de vitamine D3, mais ont egalement permis de penser que CaBP est lie au transport du calcium intracellulaire et qu'une fonction lui est attribue lors du maintien de l'homoeostasie calcique intracellulaire. Resumen Estudios experimentales sobre la calcificacion extraosea en la hipervitaminosis D3 IV. Investigaciones foto y electronicoopticas para localizar la proteina intestinal fijadora del calcio (CaBP) en codornices Se identifico la CaBP, dependiente de la vitamina D, foto y electronicoopticamente en el intestino delgado de codornices, las cuales habian vuelto a ser alimentadas, tras una deplecion que duro 17 dias, con vitamina D3 resp. avena dorada, mediante la tecnica indirecta de la inmunofluorescencia resp. inmunoperoxidasa. En la preparacion de los cortes se emplearon 3 procedimientos, el A: congelacion-descongelacion, B: liofilizacion, C: congelacion-substitucion. Se observaron dos modelos distintos de distribucion de la CaBP: el metodo A acuso una localizacion de CaBP en las celulas caliciformes, los metodos B y C lo hicieron en el citoplasma de los enterocitos y en el limbo en forma de cepillo. La interpretacion ultraestructural mostraba CaBP con el metodo A en las gotas mucosas de las celulas caliciformes, con los metodos B y C en las microvillosidades y en los ribo y polisomas, en las mitocondrias y en el ambito del tejido de los enterocitos; las celulas caliciformes y las capas subepiteliales permanecieron libres de CaBP. La interpretacion de los tres metodos empleados permite llegar a la conclusion, que la presencia de CaBP en las celulas caliciformes se debe atribuir a un artefacto condicionado por la tecnica y que la localizacion in situ verdadera del CaBP intestinal se ubica en el citoplasma de los eritrocitos. Las investigaciones no han senalado solo que la avena dorada esta en condiciones de inducir la neosintesis de CaBP, dependiente de la vitamina D, en las codornices depletadas de vitamina D3. Tambien dan pie para suponer que la CaBP participa en el transporte intracelular del calcio resp. que le corresponde una funcion en el mantenimiento de la hemoestasia intracelular calcica.
