The Experts below are selected from a list of 267 Experts worldwide ranked by ideXlab platform
Ana Elisa Kiszewski - One of the best experts on this subject based on the ideXlab platform.
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expression of cyclooxygenase type 2 in lepromatous and Tuberculoid Leprosy lesions
British Journal of Dermatology, 2003Co-Authors: Ana Elisa Kiszewski, E. Becerril, J. Baquera, Ramon Ruizmaldonado, Hernandez R PandoAbstract:SummaryBackground Leprosy is an infectious disease with two polar forms, Tuberculoid Leprosy (TL) and lepromatous Leprosy (LL), which are dominated by T-helper (Th) 1 and Th2 cells, respectively. High concentrations of prostaglandin E2 produced by the inducible enzyme cyclooxygenase type 2 (COX-2) in LL could inhibit Th1 cytokine production, contributing to T-cell anergy. Objectives To compare the COX-2 expression in LL and TL. Methods Skin biopsies from 40 Leprosy patients (LL, n = 20; TL, n = 20) were used to determine by immunohistochemistry and automated morphometry the percentage of COX-2 immunostained cells. Results Most COX-2-positive cells were macrophages; their percentages in the inflammatory infiltrate located in the papillary dermis, reticular dermis and periadnexally were significantly higher in LL than TL (P < 0·001 by Student's t-test). Conclusions The high expression of COX-2 in LL may be related to high prostaglandin production contributing to T-cell anergy.
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Expression of cyclooxygenase type 2 in lepromatous and Tuberculoid Leprosy lesions.
The British journal of dermatology, 2003Co-Authors: Ana Elisa Kiszewski, E. Becerril, J. Baquera, Ramón Ruiz-maldonado, R. Hernandez PandoAbstract:SummaryBackground Leprosy is an infectious disease with two polar forms, Tuberculoid Leprosy (TL) and lepromatous Leprosy (LL), which are dominated by T-helper (Th) 1 and Th2 cells, respectively. High concentrations of prostaglandin E2 produced by the inducible enzyme cyclooxygenase type 2 (COX-2) in LL could inhibit Th1 cytokine production, contributing to T-cell anergy. Objectives To compare the COX-2 expression in LL and TL. Methods Skin biopsies from 40 Leprosy patients (LL, n = 20; TL, n = 20) were used to determine by immunohistochemistry and automated morphometry the percentage of COX-2 immunostained cells. Results Most COX-2-positive cells were macrophages; their percentages in the inflammatory infiltrate located in the papillary dermis, reticular dermis and periadnexally were significantly higher in LL than TL (P
Ahmed Emad Mahfouz - One of the best experts on this subject based on the ideXlab platform.
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Tuberculoid Leprosy with external jugular vein thrombosis a case report and literature review
European Journal of Case Reports in Internal Medicine, 2020Co-Authors: Ali Rahil, Mohamed Magdi, Sara Kanbour, Ahmed Osman, Ahmed Emad MahfouzAbstract:: Thrombotic disease represents a rare manifestation of Leprosy. In this study, we report the case of an external jugular vein thrombosis associated with Tuberculoid Leprosy in a 23-year-old male patient. The patient presented with a 3-month history of painful cord-like swelling on the left side of the neck and a nearly 3-week history of skin lesions on the left cheek and right leg. Physical examination revealed cord-like, tender swelling on the left lateral aspect of the neck overlying the sternocleidomastoid muscle, and a hypopigmented, hypoaesthetic 6×7 cm lesion with an irregular margin on the left cheek. A Doppler ultrasound examination of the jugular vein showed thrombosis of the left external jugular vein. Three-dimensional reconstruction of the computed tomography scan showed the enlarged and enhanced left external jugular vein, as well as 1 of its tributaries, and the thickened skin patch. A skin punch biopsy from the left cheek lesion revealed granulomatous inflammation with occasional peri-adnexal granulomas, consistent with the clinical impression of Tuberculoid Leprosy. A diagnosis of Leprosy with external jugular vein thrombosis was established. Anticoagulation therapy was initiated, and the patient was referred to an infectious disease clinic for treatment with anti-Leprosy medications. LEARNING POINTS: Thickened cord-like neck swelling in Leprosy can be vein thrombosis rather than a thickened nerve.Leprosy should be considered if a skin lesion is associated with thrombosis.The common causes of upper extremity DVT.
Hernandez R Pando - One of the best experts on this subject based on the ideXlab platform.
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expression of cyclooxygenase type 2 in lepromatous and Tuberculoid Leprosy lesions
British Journal of Dermatology, 2003Co-Authors: Ana Elisa Kiszewski, E. Becerril, J. Baquera, Ramon Ruizmaldonado, Hernandez R PandoAbstract:SummaryBackground Leprosy is an infectious disease with two polar forms, Tuberculoid Leprosy (TL) and lepromatous Leprosy (LL), which are dominated by T-helper (Th) 1 and Th2 cells, respectively. High concentrations of prostaglandin E2 produced by the inducible enzyme cyclooxygenase type 2 (COX-2) in LL could inhibit Th1 cytokine production, contributing to T-cell anergy. Objectives To compare the COX-2 expression in LL and TL. Methods Skin biopsies from 40 Leprosy patients (LL, n = 20; TL, n = 20) were used to determine by immunohistochemistry and automated morphometry the percentage of COX-2 immunostained cells. Results Most COX-2-positive cells were macrophages; their percentages in the inflammatory infiltrate located in the papillary dermis, reticular dermis and periadnexally were significantly higher in LL than TL (P < 0·001 by Student's t-test). Conclusions The high expression of COX-2 in LL may be related to high prostaglandin production contributing to T-cell anergy.
R. Hernandez Pando - One of the best experts on this subject based on the ideXlab platform.
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Expression of cyclooxygenase type 2 in lepromatous and Tuberculoid Leprosy lesions.
The British journal of dermatology, 2003Co-Authors: Ana Elisa Kiszewski, E. Becerril, J. Baquera, Ramón Ruiz-maldonado, R. Hernandez PandoAbstract:SummaryBackground Leprosy is an infectious disease with two polar forms, Tuberculoid Leprosy (TL) and lepromatous Leprosy (LL), which are dominated by T-helper (Th) 1 and Th2 cells, respectively. High concentrations of prostaglandin E2 produced by the inducible enzyme cyclooxygenase type 2 (COX-2) in LL could inhibit Th1 cytokine production, contributing to T-cell anergy. Objectives To compare the COX-2 expression in LL and TL. Methods Skin biopsies from 40 Leprosy patients (LL, n = 20; TL, n = 20) were used to determine by immunohistochemistry and automated morphometry the percentage of COX-2 immunostained cells. Results Most COX-2-positive cells were macrophages; their percentages in the inflammatory infiltrate located in the papillary dermis, reticular dermis and periadnexally were significantly higher in LL than TL (P
P.j. Van Den Elsen - One of the best experts on this subject based on the ideXlab platform.
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Conserved TcR beta chain usage for a single MHC class II-restricted heat shock protein peptide.
Immunogenetics, 1998Co-Authors: Gail E. Hawes, Linda Struyk, Helen Beacock-sharp, Judy Henwood, J. S. Hill Gaston, P.j. Van Den ElsenAbstract:Previously we have shown that the T-cell response against the HLA-DR3 (17)-restricted heat shock protein (Mr 65 000)-derived peptide amino acids (aa) 3–13 (hsp65 aa 3–13) is recognized by the exclusive usage of the TRBV5 gene as well as a conserved CDR3 region in a Tuberculoid Leprosy patient. In the present study we analyzed the TcR of T-cell clones specific for hsp65 aa 3–13 derived from three healthy individuals with a response level similar to that of the Leprosy patient. We show that unlike the Tuberculoid Leprosy patient, healthy high responders have a diverse T-cell response to hsp65 aa 3–13. However, a striking observation was made: even though high responders have a diverse specific TcR repertoire, TRBV5-expressing clones from two healthy individuals could be isolated that were nearly identical to a dominant clone in the Tuberculoid Leprosy patient. In conclusion, the data show that restriction of TcR specific for an antigen correlates with the presence of that antigen in disease. However, the preferred TcR can also be detected in healthy high responders. A natural infection in vivo, as with the Tuberculoid Leprosy patient, may be responsible for the observed trimming and preferential outgrowth of a certain TcR.
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T-cell receptor beta-chain gene usage in the T-cell recognition of Mycobacterium leprae antigens in one Tuberculoid Leprosy patient.
Proceedings of the National Academy of Sciences of the United States of America, 1992Co-Authors: W. C. A. Van Schooten, R. R. P. De Vries, N. Van Der Stoep, J. B. A. G. Haanen, L. Pickering, P.j. Van Den ElsenAbstract:Abstract The beta chain of the T-cell antigen receptor present on 20 T-cell clones isolated from a Tuberculoid Leprosy patient was studied by gene rearrangement and PCR analysis. These T-cell clones all responded to Mycobacterium leprae-encoded protein antigens, and 8 of them specifically recognized peptides of the mycobacterial 65-kDa heat shock polypeptide (65hsp). All T-cell clones studied were HLA-DR-restricted (DR2 or -3). In the DR3-restricted group, 7 of 10 used a beta-chain variable region V beta 5 gene family member, whereas in the DR2-restricted group, 2 of 10 T-cell clones used a V beta 5 gene segment and 5 used the V beta 18 gene segment. The deduced amino acid sequences of the beta chain from 8 T-cell clones have revealed that 3 of 4 DR3-restricted T-cell clones expressed the V beta 5.1 gene segment whereas the fourth DR3-restricted T-cell clone employed a V beta 5 family member not previously described. The V beta 5.1-positive T-cell clones all recognized the same 65hsp peptide from residues 2 to 12. The N-D-N segment (where D is diversity) of the junctional region of these T-cell clones was very similar, despite different beta-chain joining gene segments. Of the 4 DR2-restricted T-cell clones investigated, 3 used the V beta 18 gene segment and recognized the 65hsp peptide from residues 418 to 427. In conclusion, within this panel of M. leprae-reactive T-cell clones, the DR3-restricted T-cell clones mainly used a V beta 5 gene segment, whereas the DR2-restricted clones employed preferentially the V beta 18 gene segment.
