The Experts below are selected from a list of 195 Experts worldwide ranked by ideXlab platform
Katherine Richmond - One of the best experts on this subject based on the ideXlab platform.
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Sodium dodecyl sulphate polyacrylamide gel electrophoresis patterns of Proteinuria in various renal diseases of childhood
Pediatric Nephrology, 1991Co-Authors: Trevor Brocklebank, Edward H. Cooper, Katherine RichmondAbstract:This study examined the potential of an automated electrophoretic system (PHASTSYSTEM, Pharmacia, Uppsala, Sweden) to distinguish patterns of Proteinuria in children with various renal diseases. It proved possible to produce ready-to-read sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE) separation of 1 μl of unconcentrated urine in 2 h. Glomerular, Tubular and mixed patterns of Proteinuria were identified. Steroid-responsive nephrotic syndrome (SRNS) was readily identified by strong bands of albumin and transferrin during relapses. In contrast, steroid-resistant nephrotic syndrome was associated with two additional bands of haptoglobin and IgG. Albumin dimers ( M _r 120 kDa) were found in the active phase of the disease in the urine of 90% of children with SRNS. Patterns of Tubular Proteinuria were found in children with proximal renal Tubular abnormalities. The presence of mixed patterns of glomerular and Tubular Proteinuria strongly suggest renal insufficiency. SDS PAGE electrophoresis can readily be applied in clinical practice. It may prove helpful in the diagnosis and management of children with renal diseases enabling correlation to be made between Proteinuria, renal pathology and prognosis.
Dieter Gekle - One of the best experts on this subject based on the ideXlab platform.
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Microheterogeneity of urinary albumin and Tubular Proteinuria in juvenile diabetes mellitus
Pediatric Nephrology, 1991Co-Authors: Martin Ries, Karl Schärer, Reinhard Wartha, Hildgund Schmidt, Dieter GekleAbstract:We studied differential urinary albumin excretion by a double one-dimensional gel electrophoresis with decyl sodium sulphate-polyacrylamide gel electrophoresis in the first, and isoelectric focusing in the second dimension in 37 diabetic children and 20 healthy subjects. In addition, total proteins, albumin, β_2-microglobulin and molecular size distribution of urinary proteins were measured. the latter using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Whilst albuminuria was not significantly different from controls we found an increased microheterogeneity of urinary albumin in 38% of patients. In addition, low molecular weight protein ( P
Trevor Brocklebank - One of the best experts on this subject based on the ideXlab platform.
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Sodium dodecyl sulphate polyacrylamide gel electrophoresis patterns of Proteinuria in various renal diseases of childhood
Pediatric Nephrology, 1991Co-Authors: Trevor Brocklebank, Edward H. Cooper, Katherine RichmondAbstract:This study examined the potential of an automated electrophoretic system (PHASTSYSTEM, Pharmacia, Uppsala, Sweden) to distinguish patterns of Proteinuria in children with various renal diseases. It proved possible to produce ready-to-read sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE) separation of 1 μl of unconcentrated urine in 2 h. Glomerular, Tubular and mixed patterns of Proteinuria were identified. Steroid-responsive nephrotic syndrome (SRNS) was readily identified by strong bands of albumin and transferrin during relapses. In contrast, steroid-resistant nephrotic syndrome was associated with two additional bands of haptoglobin and IgG. Albumin dimers ( M _r 120 kDa) were found in the active phase of the disease in the urine of 90% of children with SRNS. Patterns of Tubular Proteinuria were found in children with proximal renal Tubular abnormalities. The presence of mixed patterns of glomerular and Tubular Proteinuria strongly suggest renal insufficiency. SDS PAGE electrophoresis can readily be applied in clinical practice. It may prove helpful in the diagnosis and management of children with renal diseases enabling correlation to be made between Proteinuria, renal pathology and prognosis.
Jacques Reynes - One of the best experts on this subject based on the ideXlab platform.
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Long-Term Follow-Up of Proteinuria and Estimated Glomerular Filtration Rate in HIV-Infected Patients with Tubular Proteinuria
PLOS ONE, 2015Co-Authors: Hélène Peyriere, Amandine Cournil, Marie-laure Casanova, Stéphanie Badiou, Jean-paul Cristol, Jacques ReynesAbstract:Objective The objective of this prospective observational study was to describe the evolution of Tubular Proteinuria detected in HIV-infected patients, and to evaluate the impact of tenofovir disoproxil fumarate (TDF) discontinuation. Methods Proteinuria and estimated glomerular filtration rate (eGFR) were followed during a median duration of 32 months, in 81 HIV-infected patients with Tubular Proteinuria and eGFR ≥ 60 ml/min/1.73 m2 (determined using the Chronic Kidney Disease Epidemiology (CKD-EPI) Collaboration equation). Tubular Proteinuria was defined by urine protein to creatinine ratio (uPCR) ≥200 mg/g and albumin to protein ratio (uAPR)
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long term follow up of Proteinuria and estimated glomerular filtration rate in hiv infected patients with Tubular Proteinuria
PLOS ONE, 2015Co-Authors: Hélène Peyriere, Amandine Cournil, Marie-laure Casanova, Stéphanie Badiou, Jean-paul Cristol, Jacques ReynesAbstract:Objective The objective of this prospective observational study was to describe the evolution of Tubular Proteinuria detected in HIV-infected patients, and to evaluate the impact of tenofovir disoproxil fumarate (TDF) discontinuation. Methods Proteinuria and estimated glomerular filtration rate (eGFR) were followed during a median duration of 32 months, in 81 HIV-infected patients with Tubular Proteinuria and eGFR ≥ 60 ml/min/1.73 m2 (determined using the Chronic Kidney Disease Epidemiology (CKD-EPI) Collaboration equation). Tubular Proteinuria was defined by urine protein to creatinine ratio (uPCR) ≥200 mg/g and albumin to protein ratio (uAPR) <0.4. Results Twenty per cent of patients had persistence of Tubular Proteinuria: TDF continuation was the main factor associated with this persistence [OR 9.0; 95%CI: 1.9–41.4; p = 0.01]. Among the 23 patients who discontinued TDF, uPCR returned below the threshold of 200 mg/g in 11 patients. Overall, eGFR decreased with a mean rate of decline of 3.8 ml/min/1.73m2/year. The decline in eGFR was lesser after discontinuation of TDF (5.8 ml/min/1.73m2/year during TDF exposure versus 3 ml/min/1.73m2/year after TDF discontinuation; p = 0.01). Conclusions The continuation of TDF was the main factor associated with the persistence of Proteinuria. Moreover, Proteinuria was normalized in only half of the patients who discontinued TDF. The clinical significance of TDF-related low level of Proteinuria as a factor associated with renal disease progression and bone loss remains poorly understood.
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Tubular and glomerular Proteinuria in hiv infected adults with estimated glomerular filtration rate 60 ml min per 1 73 m2
AIDS, 2013Co-Authors: Jacques Reynes, Hélène Peyriere, Amandine Cournil, Jean-paul Cristol, Christina Psomas, Elsa Guiller, Marlene Chatron, Stéphanie BadiouAbstract:OBJECTIVE: To assess the frequency of glomerular and Tubular Proteinuria in a cohort of HIV-infected patients, and to determine the factors associated with each type of injury. DESIGN: Cross-sectional study of 1210 consecutive HIV-infected adults followed in HIV outpatient unit (Montpellier/France). METHODS: Spot urine protein to creatinine (uPCR), albumin to creatinine (uACR) and albumin to protein (uAPR) ratios were assessed. Glomerular injury was defined as uACR at least 30 mg/g or uPCR at least 200 mg/g with uAPR at least 0.4. Tubular injury was defined as uPCR 200 mg/g or more with uAPR less than 0.4. Multivariate logistic regression identified independent factors of each type of Proteinuria, in the 1158 patients with estimated glomerular filtration rate (eGFR) at least 60 ml/min per 1.73 m, using re-expressed modification of diet in renal disease equation. RESULTS: Frequency of Proteinuria was 18.2% among patients with eGFR at least 60 ml/min per 1.73 m consisting in Tubular Proteinuria for 50.7% of them. Factors associated with glomerular Proteinuria were age [OR 1.34/10-year increment (95%CI: 1.08-1.66)], diabetes [OR 3.37 (95%CI: 1.53-7.44)], and arterial hypertension [OR 2.52 (95%CI: 1.36-4.66)]. Factors associated with Tubular Proteinuria were age [OR 1.43 (95%CI: 1.14-1.79)], current tenofovir use [OR 3.52 (95%CI: 1.86-6.65)], hepatitis C co-infection [OR 1.62 (95%CI: 1.00-2.65)], AIDS stage [OR 1.83 (95%CI: 1.18-2.82)], CD4 cell count less than 200 per μl [OR 2.48 (95%CI: 1.31-4.70)]. CONCLUSION: This study distinguished risk factors for Tubular injury, mainly related to HIV disease and its treatment (tenofovir), and glomerular injury, linked to non HIV-related variables (age, diabetes, hypertension). Measuring uPCR, uACR and uAPR may help with the detection and specific management of early chronic kidney disease in HIV-infected patients having normal or sub-normal eGFR.
Michael J. Dillon - One of the best experts on this subject based on the ideXlab platform.
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Tubular Proteinuria in reflux nephropathy: post ureteric re-implantation
Pediatric Nephrology, 1996Co-Authors: Chulananda D. A. Goonasekera, Vanita Shah, Michael J. DillonAbstract:We studied urine protein excretion in 55 adults with reflux nephropathy (median age 26.9 years) who had had normal blood pressure, renal function and ureteric reimplantation in childhood. Urine retinol binding protein (RBP), N -acetyl-β- d -glucosaminidase (NAG), albumin, bacteriuria, systolic blood pressure, glomerular filtration rate (GFR), peripheral plasma renin activity (PRA) and the degree of renal scarring were measured in each subject; 20 had bilateral and 35 unilateral renal scarring; 5 were hypertensive and none were in renal failure. Urinary NAG and RBP excretions were significantly greater in the study group than in 34 healthy controls (median age 29.7 years). Within the study group, NAG excretion significantly correlated with PRA ( P =0.02). RBP excretion correlated with PRA, systolic blood pressure and the laterality (bilateral vs. unilateral) of scarring ( P
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Tubular Proteinuria in reflux nephropathy post ureteric re implantation
Pediatric Nephrology, 1996Co-Authors: Chulananda D. A. Goonasekera, Vanita Shah, Michael J. DillonAbstract:We studied urine protein excretion in 55 adults with reflux nephropathy (median age 26.9 years) who had had normal blood pressure, renal function and ureteric reimplantation in childhood. Urine retinol binding protein (RBP), N-acetyl-β-d-glucosaminidase (NAG), albumin, bacteriuria, systolic blood pressure, glomerular filtration rate (GFR), peripheral plasma renin activity (PRA) and the degree of renal scarring were measured in each subject; 20 had bilateral and 35 unilateral renal scarring; 5 were hypertensive and none were in renal failure. Urinary NAG and RBP excretions were significantly greater in the study group than in 34 healthy controls (median age 29.7 years). Within the study group, NAG excretion significantly correlated with PRA (P=0.02). RBP excretion correlated with PRA, systolic blood pressure and the laterality (bilateral vs. unilateral) of scarring (P<0.01). Urinary albumin excretion correlated with systolic blood pressure (P=0.03). We conclude that increase urinary protein, especially NAG and RBP excretion, occur late after ureteric re-implantation in reflux nephropathy independent of GFR. Its association with PRA supports the concept of segmental perfusion and filtration as an important mechanism that may explain the above findings.
