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Eva Klein - One of the best experts on this subject based on the ideXlab platform.
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hla b5 restricted auto Tumor specific cytotoxic t Cells generated in mixed lymphocyte Tumor Cell Culture
International Journal of Cancer, 1992Co-Authors: P Wang, Farkas Vánky, Zsuzsa Vegh, Eva KleinAbstract:T-Cell-enriched lymphocyte populations derived from the malignant exudate of a patient with ovarian carcinoma were exposed to autologous Tumor Cells in the mixed lymphocyte-Tumor-Cell Culture (MLTC) and propagated for 42 days. Proliferation of lymphocytes depended on exposures to autologous Tumor Cells and on the presence of IL-2. After 7 days, the MLTC-lymphocytes lysed K562 and the autologous Tumor Cells. The latter effect was not inhibited by monoclonal antibodies (MAbs) reactive with MHC class-1 antigens or with CD3. After 7 restimulations, the Culture was enriched in CD8+ Cells (92%) and showed selective lytic activity against the autologous Tumor. This function was inhibited by the α-class 1 or α-CD3 MAbs, and also by antibodies reactive with the HLA B locus or B5 allele products. The antibodies reactive with HLA A molecules had no such effect. It seems therefore that the function of the CTLs was restricted by HLA B5. Analysis of the TCRβ genes indicated clonal T-Cell expansion in this Culture. This MLTC was I of 21 initiated with 11 blood-and 10 Tumor-derived lymphocyte (TIL) populations prepared from the malignant effusions of ovarian carcinoma patients. None of these ex-vivo lymphocytes lysed autologous Tumor Cells. In 17 MLTCs the lymphocytes did not proliferate, and in 3 Cultures the proliferation was maintained only for 2–3 weeks. In 3 of 4 Cultures auto-Tumor cytotoxicity was induced. © 1992 Wiley-Liss, Inc.
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mhc class i restricted auto Tumor specific cd4 cd8 t Cell clones established from autologous mixed lymphocyte Tumor Cell Culture mltc
International Journal of Cancer, 1992Co-Authors: P Wang, Farkas Vánky, Eva KleinAbstract:: Autologous mixed lymphocyte-Tumor Cell Cultures (MLTC) were initiated with cytokine (IFN gamma and TNF alpha)-treated ex-vivo Tumor Cells of lung, ovarian, breast and stomach carcinomas. The cytokine-treated Tumors expressed class-I but not class-II molecules. Although the proportion of CD8+ lymphocytes increased in the bulk Culture of MLTCs, in 5/7 experiments the majority of the established T-Cell clones were CD4+. Among the CD8+ clones a high proportion (77%) was cytotoxic, while the proliferative response was more frequent among the CD4+ clones (70%). In 4/26 cytotoxic T-lymphocyte (CTL) clones (3/17 CD4+ and 1/9 CD8+), derived from a patient with class I+ class II- stomach carcinoma, lysis was restricted to the autologous Tumor Cells. These auto-Tumor-specific clones did not lyse the autologous ConA blasts, the 5 allogeneic ex-vivo Tumors, the NK-sensitive K562 or the relatively sensitive Daudi Cells. The cytotoxicity of these clones was inhibited by pre-incubation of the Tumor Cells with W6/32 (alpha-class I) MAb, or by preincubation of the lymphocytes with OKT3 (alpha-CD3) MAb. The alpha-CD4 (OKT4) MAb had only a marginal effect on the CD4+ clones, while the lytic function of the CD8+ clone was inhibited by the alpha-CD8 (OKT8) MAb. The 3 CD4+ CTL clones also responded with proliferation to the autologous Tumor Cells. This proliferative response was inhibited by the presence of W6/32 MAb. Our results indicate that the auto-Tumor lysis exerted by CD4+ CTL clones was restricted by the class-I antigens, and that the CD4 molecules of the clones were not essential for the lytic interaction.
P Wang - One of the best experts on this subject based on the ideXlab platform.
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hla b5 restricted auto Tumor specific cytotoxic t Cells generated in mixed lymphocyte Tumor Cell Culture
International Journal of Cancer, 1992Co-Authors: P Wang, Farkas Vánky, Zsuzsa Vegh, Eva KleinAbstract:T-Cell-enriched lymphocyte populations derived from the malignant exudate of a patient with ovarian carcinoma were exposed to autologous Tumor Cells in the mixed lymphocyte-Tumor-Cell Culture (MLTC) and propagated for 42 days. Proliferation of lymphocytes depended on exposures to autologous Tumor Cells and on the presence of IL-2. After 7 days, the MLTC-lymphocytes lysed K562 and the autologous Tumor Cells. The latter effect was not inhibited by monoclonal antibodies (MAbs) reactive with MHC class-1 antigens or with CD3. After 7 restimulations, the Culture was enriched in CD8+ Cells (92%) and showed selective lytic activity against the autologous Tumor. This function was inhibited by the α-class 1 or α-CD3 MAbs, and also by antibodies reactive with the HLA B locus or B5 allele products. The antibodies reactive with HLA A molecules had no such effect. It seems therefore that the function of the CTLs was restricted by HLA B5. Analysis of the TCRβ genes indicated clonal T-Cell expansion in this Culture. This MLTC was I of 21 initiated with 11 blood-and 10 Tumor-derived lymphocyte (TIL) populations prepared from the malignant effusions of ovarian carcinoma patients. None of these ex-vivo lymphocytes lysed autologous Tumor Cells. In 17 MLTCs the lymphocytes did not proliferate, and in 3 Cultures the proliferation was maintained only for 2–3 weeks. In 3 of 4 Cultures auto-Tumor cytotoxicity was induced. © 1992 Wiley-Liss, Inc.
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mhc class i restricted auto Tumor specific cd4 cd8 t Cell clones established from autologous mixed lymphocyte Tumor Cell Culture mltc
International Journal of Cancer, 1992Co-Authors: P Wang, Farkas Vánky, Eva KleinAbstract:: Autologous mixed lymphocyte-Tumor Cell Cultures (MLTC) were initiated with cytokine (IFN gamma and TNF alpha)-treated ex-vivo Tumor Cells of lung, ovarian, breast and stomach carcinomas. The cytokine-treated Tumors expressed class-I but not class-II molecules. Although the proportion of CD8+ lymphocytes increased in the bulk Culture of MLTCs, in 5/7 experiments the majority of the established T-Cell clones were CD4+. Among the CD8+ clones a high proportion (77%) was cytotoxic, while the proliferative response was more frequent among the CD4+ clones (70%). In 4/26 cytotoxic T-lymphocyte (CTL) clones (3/17 CD4+ and 1/9 CD8+), derived from a patient with class I+ class II- stomach carcinoma, lysis was restricted to the autologous Tumor Cells. These auto-Tumor-specific clones did not lyse the autologous ConA blasts, the 5 allogeneic ex-vivo Tumors, the NK-sensitive K562 or the relatively sensitive Daudi Cells. The cytotoxicity of these clones was inhibited by pre-incubation of the Tumor Cells with W6/32 (alpha-class I) MAb, or by preincubation of the lymphocytes with OKT3 (alpha-CD3) MAb. The alpha-CD4 (OKT4) MAb had only a marginal effect on the CD4+ clones, while the lytic function of the CD8+ clone was inhibited by the alpha-CD8 (OKT8) MAb. The 3 CD4+ CTL clones also responded with proliferation to the autologous Tumor Cells. This proliferative response was inhibited by the presence of W6/32 MAb. Our results indicate that the auto-Tumor lysis exerted by CD4+ CTL clones was restricted by the class-I antigens, and that the CD4 molecules of the clones were not essential for the lytic interaction.
Farkas Vánky - One of the best experts on this subject based on the ideXlab platform.
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hla b5 restricted auto Tumor specific cytotoxic t Cells generated in mixed lymphocyte Tumor Cell Culture
International Journal of Cancer, 1992Co-Authors: P Wang, Farkas Vánky, Zsuzsa Vegh, Eva KleinAbstract:T-Cell-enriched lymphocyte populations derived from the malignant exudate of a patient with ovarian carcinoma were exposed to autologous Tumor Cells in the mixed lymphocyte-Tumor-Cell Culture (MLTC) and propagated for 42 days. Proliferation of lymphocytes depended on exposures to autologous Tumor Cells and on the presence of IL-2. After 7 days, the MLTC-lymphocytes lysed K562 and the autologous Tumor Cells. The latter effect was not inhibited by monoclonal antibodies (MAbs) reactive with MHC class-1 antigens or with CD3. After 7 restimulations, the Culture was enriched in CD8+ Cells (92%) and showed selective lytic activity against the autologous Tumor. This function was inhibited by the α-class 1 or α-CD3 MAbs, and also by antibodies reactive with the HLA B locus or B5 allele products. The antibodies reactive with HLA A molecules had no such effect. It seems therefore that the function of the CTLs was restricted by HLA B5. Analysis of the TCRβ genes indicated clonal T-Cell expansion in this Culture. This MLTC was I of 21 initiated with 11 blood-and 10 Tumor-derived lymphocyte (TIL) populations prepared from the malignant effusions of ovarian carcinoma patients. None of these ex-vivo lymphocytes lysed autologous Tumor Cells. In 17 MLTCs the lymphocytes did not proliferate, and in 3 Cultures the proliferation was maintained only for 2–3 weeks. In 3 of 4 Cultures auto-Tumor cytotoxicity was induced. © 1992 Wiley-Liss, Inc.
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mhc class i restricted auto Tumor specific cd4 cd8 t Cell clones established from autologous mixed lymphocyte Tumor Cell Culture mltc
International Journal of Cancer, 1992Co-Authors: P Wang, Farkas Vánky, Eva KleinAbstract:: Autologous mixed lymphocyte-Tumor Cell Cultures (MLTC) were initiated with cytokine (IFN gamma and TNF alpha)-treated ex-vivo Tumor Cells of lung, ovarian, breast and stomach carcinomas. The cytokine-treated Tumors expressed class-I but not class-II molecules. Although the proportion of CD8+ lymphocytes increased in the bulk Culture of MLTCs, in 5/7 experiments the majority of the established T-Cell clones were CD4+. Among the CD8+ clones a high proportion (77%) was cytotoxic, while the proliferative response was more frequent among the CD4+ clones (70%). In 4/26 cytotoxic T-lymphocyte (CTL) clones (3/17 CD4+ and 1/9 CD8+), derived from a patient with class I+ class II- stomach carcinoma, lysis was restricted to the autologous Tumor Cells. These auto-Tumor-specific clones did not lyse the autologous ConA blasts, the 5 allogeneic ex-vivo Tumors, the NK-sensitive K562 or the relatively sensitive Daudi Cells. The cytotoxicity of these clones was inhibited by pre-incubation of the Tumor Cells with W6/32 (alpha-class I) MAb, or by preincubation of the lymphocytes with OKT3 (alpha-CD3) MAb. The alpha-CD4 (OKT4) MAb had only a marginal effect on the CD4+ clones, while the lytic function of the CD8+ clone was inhibited by the alpha-CD8 (OKT8) MAb. The 3 CD4+ CTL clones also responded with proliferation to the autologous Tumor Cells. This proliferative response was inhibited by the presence of W6/32 MAb. Our results indicate that the auto-Tumor lysis exerted by CD4+ CTL clones was restricted by the class-I antigens, and that the CD4 molecules of the clones were not essential for the lytic interaction.
Vincent E C Ooi - One of the best experts on this subject based on the ideXlab platform.
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carboxymethylated β glucans from mushroom sclerotium of pleurotus tuber regium as novel water soluble anti Tumor agent
Carbohydrate Polymers, 2004Co-Authors: Mei Zhang, Peter C K Cheung, Lina Zhang, Chiming Chiu, Vincent E C OoiAbstract:Six hot alkali extracts (HAE-1 to HAE-6) of mushroom (1→3)-β-glucans from the sclerotia of Pleurotus tuber-regium having different molecular weight (Mw) ranging from 1×104 to 42×104 were carboxymethylated to give their corresponding water-soluble derivatives (CMHAE-1 to CMHAE-6) with Mw ranged from 2.08×104 to 53.2×104. In general, the CMHAE β-glucans had higher water solubility and higher in vivo (Sarcoma 180 solid Tumor implanted on BALB/c mice) as well as in vitro (HL-60 Tumor Cell Culture) anti-Tumor activity than the native HAE β-glucans. From ELISA assay of cytokines, CMHAE fractions could increase production of Tumor necrosis factor alpha (TNF-α) in mouse plasma stimulated by lipopolysaccharide (LPS). It was postulated that a more expanded flexible chain of these novel CMHAE β-glucans might account for their in vivo and in vitro anti-Tumor activities.
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molecular weight and anti Tumor activity of the water soluble polysaccharides isolated by hot water and ultrasonic treatment from the sclerotia and mycelia of pleurotus tuber regium
Carbohydrate Polymers, 2004Co-Authors: Mei Zhang, Peter C K Cheung, Lina Zhang, Vincent E C OoiAbstract:The sclerotia (S) and mycelia (M) of Pleurotus tuber regium were extracted, respectively, by hot water (h) and ultrasonication (s) to yield four water-soluble polysaccharides coded as Sh, Mh, Ss and Ms. All water-soluble polysaccharides were mixture of two fractions that contained predominantly polysaccharides with glucose as the major sugar and galactose and mannose as the minor component. The water-soluble extracts from mycelia seemed to have higher protein content than the ones from sclerotia. The ultrasonicated water extracts (Ms and Ss) contained glycan–chitin complexes with higher weight-average molecular weight (Mw) than that by hot water. Interestingly, the polysaccharides Mh and Sh extracted with hot water, which contained the major fractions with Mw in the range of 40–80×104, exhibited stronger in vivo (Sarcoma 180 solid Tumor implanted in BALB/c mice) and in vitro (HL-60 Tumor Cell Culture) anti-Tumor activities than that obtained by ultrasonication. In view of these results, the anti-Tumor activities of the water-soluble polysaccharides isolated from both sclerotia and mycelia of P. tuber-regium depended on the method of isolation that affected the Mw profile and conponent of the extracts. The effects of moderate Mw and protein content of the polysaccharides on the improvement of the bioactivities could not be negligible.
Hugo Alexandre Oliveira Rocha - One of the best experts on this subject based on the ideXlab platform.
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antioxidant and antiproliferative activities of methanolic extract from a neglected agricultural product corn cobs
Molecules, 2014Co-Authors: Raniere Fagundes Melosilveira, Gabriel Pereira Fidelis, Rony Lucas Silva Viana, Vinicius Campelo Soeiro, Rodrigo A Da Silva, Daisy Machado, Leandro Silva Costa, Carmen Verissima Ferreira, Hugo Alexandre Oliveira RochaAbstract:Neglected agricultural products (NAPs) are defined as discarded material in agricultural production. Corn cobs are a major waste of agriCulture maize. Here, a methanolic extract from corn cobs (MEC) was obtained. MEC contains phenolic compounds, protein, carbohydrates (1.4:0.001:0.001). We evaluated the in vitro and in vivo antioxidant potential of MEC. Furthermore, its antiproliferative property against Tumor Cells was assessed through MTT assays and proteins related to apoptosis in Tumor Cells were examined by western blot. MEC showed no hydroxyl radical scavenger capacity, but it showed antioxidant activity in Total Antioxidant Capacity and DPPH scavenger ability assays. MEC showed higher Reducing Power than ascorbic acid and exhibited high Superoxide Scavenging activity. In Tumor Cell Culture, MEC increased catalase, metallothionein and superoxide dismutase expression in accordance with the antioxidant tests. In vivo antioxidant test, MEC restored SOD and CAT, decreased malondialdehyde activities and showed high Trolox Equivalent Antioxidant Capacity in animals treated with CCl4. Furthermore, MEC decreased HeLa Cells viability by apoptosis due an increase of Bax/Bcl-2 ratio, caspase 3 active. Protein kinase C expression increased was also detected in treated Tumor Cells. Thus, our findings pointed out the biotechnological potential of corn cobs as a source of molecules with pharmacological activity.
