The Experts below are selected from a list of 234 Experts worldwide ranked by ideXlab platform
Ryoichi Takayanagi - One of the best experts on this subject based on the ideXlab platform.
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establishment and characterization of a steroidogenic human granulosa like Tumor Cell Line kgn that expresses functional follicle stimulating hormone receptor
Endocrinology, 2001Co-Authors: Yoshihiro Nishi, Toshihiko Yanase, Yiming Mu, Isao Ichino, Masayuki Saito, Masatoshi Nomura, Chizu Mukasa, Taijiro Okabe, Kiminobu Goto, Ryoichi TakayanagiAbstract:We established a steroidogenic human ovarian granulosa-like Tumor Cell Line, designated KGN, from a patient with invasive ovarian granulosa Cell carcinoma. KGN had a relatively long population doubling time of about 46.4 h and had an abnormal karyotype of 45,XX, 7q-, -22. A steroid analysis of the cultured medium by RIA performed 5 yr after the initiation of culture showed that KGN was able to secrete pregnenolone and progesterone, and both dramatically increased after stimulation with (Bu)2cAMP. However, little or no secretion of 17α-hydroxylated steroids, dehydroepiandrosterone, androstenedione, or estradiol was observed. The aromatase activity of KGN was relatively high and was further stimulated by (Bu)2cAMP or FSH. These findings showed a pattern similar to that of steroidogenesis in human granulosa Cells, thus allowing analysis of naturally occurring steroidogenesis in human granulosa Cells. Fas-mediated apoptosis of KGN was also observed, which mimicked the physiological regulation of apoptosis in ...
Toshihiko Yanase - One of the best experts on this subject based on the ideXlab platform.
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a benzimidazole fungicide benomyl and its metabolite carbendazim induce aromatase activity in a human ovarian granulose like Tumor Cell Line kgn
Endocrinology, 2004Co-Authors: Hidetaka Morinaga, Toshihiko Yanase, Masatoshi Nomura, Taijiro Okabe, Kiminobu Goto, Nobuhiro Harada, Hajime NawataAbstract:Endocrine disruptor chemicals are known to cause a range of abnormalities in sexual differentiation and reproduction. One mechanism underlying such effects may be via alteration of aromatase activity, which is responsible for estrogen production. A good screening system for identifying endocrine disruptors has long been desired. We have recently established a human ovarian granulosa-like Tumor Cell Line, KGN, which possesses a relatively high level of aromatase expression and is considered a useful mammalian model for investigating the in vitro effects of various chemicals on aromatase activity. In this study we screened 55 different candidate chemicals for endocrine disruptors by assaying aromatase activity. Only benomyl, known as both a benzimidazole fungicide and a microtubule-interfering agent, was found to induce aromatase activity in association with increased levels of aromatase mRNA in KGN Cells. The effect of benomyl was presumed to be mediated by its metabolite carbendazim, because it produced a...
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establishment and characterization of a steroidogenic human granulosa like Tumor Cell Line kgn that expresses functional follicle stimulating hormone receptor
Endocrinology, 2001Co-Authors: Yoshihiro Nishi, Toshihiko Yanase, Yiming Mu, Isao Ichino, Masayuki Saito, Masatoshi Nomura, Chizu Mukasa, Taijiro Okabe, Kiminobu Goto, Ryoichi TakayanagiAbstract:We established a steroidogenic human ovarian granulosa-like Tumor Cell Line, designated KGN, from a patient with invasive ovarian granulosa Cell carcinoma. KGN had a relatively long population doubling time of about 46.4 h and had an abnormal karyotype of 45,XX, 7q-, -22. A steroid analysis of the cultured medium by RIA performed 5 yr after the initiation of culture showed that KGN was able to secrete pregnenolone and progesterone, and both dramatically increased after stimulation with (Bu)2cAMP. However, little or no secretion of 17α-hydroxylated steroids, dehydroepiandrosterone, androstenedione, or estradiol was observed. The aromatase activity of KGN was relatively high and was further stimulated by (Bu)2cAMP or FSH. These findings showed a pattern similar to that of steroidogenesis in human granulosa Cells, thus allowing analysis of naturally occurring steroidogenesis in human granulosa Cells. Fas-mediated apoptosis of KGN was also observed, which mimicked the physiological regulation of apoptosis in ...
Masatoshi Nomura - One of the best experts on this subject based on the ideXlab platform.
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a benzimidazole fungicide benomyl and its metabolite carbendazim induce aromatase activity in a human ovarian granulose like Tumor Cell Line kgn
Endocrinology, 2004Co-Authors: Hidetaka Morinaga, Toshihiko Yanase, Masatoshi Nomura, Taijiro Okabe, Kiminobu Goto, Nobuhiro Harada, Hajime NawataAbstract:Endocrine disruptor chemicals are known to cause a range of abnormalities in sexual differentiation and reproduction. One mechanism underlying such effects may be via alteration of aromatase activity, which is responsible for estrogen production. A good screening system for identifying endocrine disruptors has long been desired. We have recently established a human ovarian granulosa-like Tumor Cell Line, KGN, which possesses a relatively high level of aromatase expression and is considered a useful mammalian model for investigating the in vitro effects of various chemicals on aromatase activity. In this study we screened 55 different candidate chemicals for endocrine disruptors by assaying aromatase activity. Only benomyl, known as both a benzimidazole fungicide and a microtubule-interfering agent, was found to induce aromatase activity in association with increased levels of aromatase mRNA in KGN Cells. The effect of benomyl was presumed to be mediated by its metabolite carbendazim, because it produced a...
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establishment and characterization of a steroidogenic human granulosa like Tumor Cell Line kgn that expresses functional follicle stimulating hormone receptor
Endocrinology, 2001Co-Authors: Yoshihiro Nishi, Toshihiko Yanase, Yiming Mu, Isao Ichino, Masayuki Saito, Masatoshi Nomura, Chizu Mukasa, Taijiro Okabe, Kiminobu Goto, Ryoichi TakayanagiAbstract:We established a steroidogenic human ovarian granulosa-like Tumor Cell Line, designated KGN, from a patient with invasive ovarian granulosa Cell carcinoma. KGN had a relatively long population doubling time of about 46.4 h and had an abnormal karyotype of 45,XX, 7q-, -22. A steroid analysis of the cultured medium by RIA performed 5 yr after the initiation of culture showed that KGN was able to secrete pregnenolone and progesterone, and both dramatically increased after stimulation with (Bu)2cAMP. However, little or no secretion of 17α-hydroxylated steroids, dehydroepiandrosterone, androstenedione, or estradiol was observed. The aromatase activity of KGN was relatively high and was further stimulated by (Bu)2cAMP or FSH. These findings showed a pattern similar to that of steroidogenesis in human granulosa Cells, thus allowing analysis of naturally occurring steroidogenesis in human granulosa Cells. Fas-mediated apoptosis of KGN was also observed, which mimicked the physiological regulation of apoptosis in ...
Taijiro Okabe - One of the best experts on this subject based on the ideXlab platform.
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a benzimidazole fungicide benomyl and its metabolite carbendazim induce aromatase activity in a human ovarian granulose like Tumor Cell Line kgn
Endocrinology, 2004Co-Authors: Hidetaka Morinaga, Toshihiko Yanase, Masatoshi Nomura, Taijiro Okabe, Kiminobu Goto, Nobuhiro Harada, Hajime NawataAbstract:Endocrine disruptor chemicals are known to cause a range of abnormalities in sexual differentiation and reproduction. One mechanism underlying such effects may be via alteration of aromatase activity, which is responsible for estrogen production. A good screening system for identifying endocrine disruptors has long been desired. We have recently established a human ovarian granulosa-like Tumor Cell Line, KGN, which possesses a relatively high level of aromatase expression and is considered a useful mammalian model for investigating the in vitro effects of various chemicals on aromatase activity. In this study we screened 55 different candidate chemicals for endocrine disruptors by assaying aromatase activity. Only benomyl, known as both a benzimidazole fungicide and a microtubule-interfering agent, was found to induce aromatase activity in association with increased levels of aromatase mRNA in KGN Cells. The effect of benomyl was presumed to be mediated by its metabolite carbendazim, because it produced a...
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establishment and characterization of a steroidogenic human granulosa like Tumor Cell Line kgn that expresses functional follicle stimulating hormone receptor
Endocrinology, 2001Co-Authors: Yoshihiro Nishi, Toshihiko Yanase, Yiming Mu, Isao Ichino, Masayuki Saito, Masatoshi Nomura, Chizu Mukasa, Taijiro Okabe, Kiminobu Goto, Ryoichi TakayanagiAbstract:We established a steroidogenic human ovarian granulosa-like Tumor Cell Line, designated KGN, from a patient with invasive ovarian granulosa Cell carcinoma. KGN had a relatively long population doubling time of about 46.4 h and had an abnormal karyotype of 45,XX, 7q-, -22. A steroid analysis of the cultured medium by RIA performed 5 yr after the initiation of culture showed that KGN was able to secrete pregnenolone and progesterone, and both dramatically increased after stimulation with (Bu)2cAMP. However, little or no secretion of 17α-hydroxylated steroids, dehydroepiandrosterone, androstenedione, or estradiol was observed. The aromatase activity of KGN was relatively high and was further stimulated by (Bu)2cAMP or FSH. These findings showed a pattern similar to that of steroidogenesis in human granulosa Cells, thus allowing analysis of naturally occurring steroidogenesis in human granulosa Cells. Fas-mediated apoptosis of KGN was also observed, which mimicked the physiological regulation of apoptosis in ...
Kiminobu Goto - One of the best experts on this subject based on the ideXlab platform.
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a benzimidazole fungicide benomyl and its metabolite carbendazim induce aromatase activity in a human ovarian granulose like Tumor Cell Line kgn
Endocrinology, 2004Co-Authors: Hidetaka Morinaga, Toshihiko Yanase, Masatoshi Nomura, Taijiro Okabe, Kiminobu Goto, Nobuhiro Harada, Hajime NawataAbstract:Endocrine disruptor chemicals are known to cause a range of abnormalities in sexual differentiation and reproduction. One mechanism underlying such effects may be via alteration of aromatase activity, which is responsible for estrogen production. A good screening system for identifying endocrine disruptors has long been desired. We have recently established a human ovarian granulosa-like Tumor Cell Line, KGN, which possesses a relatively high level of aromatase expression and is considered a useful mammalian model for investigating the in vitro effects of various chemicals on aromatase activity. In this study we screened 55 different candidate chemicals for endocrine disruptors by assaying aromatase activity. Only benomyl, known as both a benzimidazole fungicide and a microtubule-interfering agent, was found to induce aromatase activity in association with increased levels of aromatase mRNA in KGN Cells. The effect of benomyl was presumed to be mediated by its metabolite carbendazim, because it produced a...
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establishment and characterization of a steroidogenic human granulosa like Tumor Cell Line kgn that expresses functional follicle stimulating hormone receptor
Endocrinology, 2001Co-Authors: Yoshihiro Nishi, Toshihiko Yanase, Yiming Mu, Isao Ichino, Masayuki Saito, Masatoshi Nomura, Chizu Mukasa, Taijiro Okabe, Kiminobu Goto, Ryoichi TakayanagiAbstract:We established a steroidogenic human ovarian granulosa-like Tumor Cell Line, designated KGN, from a patient with invasive ovarian granulosa Cell carcinoma. KGN had a relatively long population doubling time of about 46.4 h and had an abnormal karyotype of 45,XX, 7q-, -22. A steroid analysis of the cultured medium by RIA performed 5 yr after the initiation of culture showed that KGN was able to secrete pregnenolone and progesterone, and both dramatically increased after stimulation with (Bu)2cAMP. However, little or no secretion of 17α-hydroxylated steroids, dehydroepiandrosterone, androstenedione, or estradiol was observed. The aromatase activity of KGN was relatively high and was further stimulated by (Bu)2cAMP or FSH. These findings showed a pattern similar to that of steroidogenesis in human granulosa Cells, thus allowing analysis of naturally occurring steroidogenesis in human granulosa Cells. Fas-mediated apoptosis of KGN was also observed, which mimicked the physiological regulation of apoptosis in ...