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Yongping Yang - One of the best experts on this subject based on the ideXlab platform.
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brrice1 1 is associated with putrescine synthesis through regulation of the arginine decarboxylase gene in freezing tolerance of Turnip brassica rapa var rapa
BMC Plant Biology, 2020Co-Authors: Xin Yin, Yongping Yang, Yunqiang Yang, Danni Yang, Yanling YueAbstract:In the agricultural areas of Qinghai-Tibet Plateau, temperature varies widely from day to night during the growing season, which makes the extreme temperature become one of the limiting factors of crop yield. Turnip (Brassica rapa var. rapa) is a traditional crop of Tibet grown in the Tibet Plateau, but its molecular and metabolic mechanisms of freezing tolerance are unclear. Here, based on the changes in transcriptional and metabolic levels of Tibetan Turnip under freezing treatment, the expression of the arginine decarboxylase gene BrrADC2.2 exhibited an accumulative pattern in accordance with putrescine content. Moreover, we demonstrated that BrrICE1.1 (Inducer of CBF Expression 1) could directly bind to the BrrADC2.2 promoter, activating BrrADC2.2 to promote the accumulation of putrescine, which was verified by RNAi and overexpression analyses for both BrrADC2.2 and BrrICE1.1 using transgenic hair root. The function of putrescine in Turnip was further analyzed by exogenous application putrescine and its inhibitor DL-α-(Difluoromethyl) arginine (DFMA) under freezing tolerance. In addition, the BrrICE1.1 was found to be involved in the ICE1-CBF pathway to increase the freezing stress of Turnip. BrrICE1.1 could bind the promoter of BrrADC2.2 or CBFs to participate in freezing tolerance of Turnip by transcriptomics and targeted metabolomics analyses. This study revealed the regulatory network of the freezing tolerance process in Turnip and increased our understanding of the plateau crops response to extreme environments in Tibet.
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brrice1 1 is associated with putrescine synthesis through regulation of the arginine decarboxylase gene in freezing tolerance of Turnip brassica rapa var rapa
BMC Plant Biology, 2020Co-Authors: Xin Yin, Yongping Yang, Yunqiang Yang, Danni Yang, Yanling YueAbstract:Background In the agricultural areas of Qinghai-Tibet Plateau, temperature varies widely from day to night during the growing season, which makes the extreme temperature become one of the limiting factors of crop yield. Turnip (Brassica rapa var. rapa) is a traditional crop of Tibet grown in the Tibet Plateau, but its molecular and metabolic mechanisms of freezing tolerance are unclear. Results Here, based on the changes in transcriptional and metabolic levels of Tibetan Turnip under freezing treatment, the expression of the arginine decarboxylase gene BrrADC2.2 exhibited an accumulative pattern in accordance with putrescine content. Moreover, we demonstrated that BrrICE1.1 (Inducer of CBF Expression 1) could directly bind to the BrrADC2.2 promoter, activating BrrADC2.2 to promote the accumulation of putrescine, which was verified by RNAi and overexpression analyses for both BrrADC2.2 and BrrICE1.1 using transgenic hair root. The function of putrescine in Turnip was further analyzed by exogenous application putrescine and its inhibitor DL-α-(Difluoromethyl) arginine (DFMA) under freezing tolerance. In addition, the BrrICE1.1 was found to be involved in the ICE1-CBF pathway to increase the freezing stress of Turnip. Conclusions BrrICE1.1 could bind the promoter of BrrADC2.2 or CBFs to participate in freezing tolerance of Turnip by transcriptomics and targeted metabolomics analyses. This study revealed the regulatory network of the freezing tolerance process in Turnip and increased our understanding of the plateau crops response to extreme environments in Tibet.
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comparative study of the glucosinolate profiles in Turnip from four agroclimatic zones of china and neighboring countries
Journal of Food Measurement and Characterization, 2019Co-Authors: Swastika Paul, Changa Geng, Tonghua Yang, Yongping Yang, Jiju CheAbstract:Turnip (Brassica rapa) is an important crop cultivated all over the world. Glucosinolates as the characteristic constituents showed diverse bioactivities, especially for their anti-cancer and hepatoprotective effects. Plenty of investigation has revealed the glucosinolate contents and profiles in various Brassica vegetables (mainly on aerial parts), whereas their detailed comparison between different agroclimatic zones is rarely reported. This study aimed to make a comparison on the total glucosinolate contents (TGCs) and chemical profiles in 59 Turnip landraces. The Turnip tubers were obtained by collecting the seeds from four agroclimatic zones, Yunnan, Himalayas, Qinghai-Tibetan Plateau (QTP) and Xinjiang, around southwest China and sown in Jianshui County. The TGCs in Turnip were high (TGCYunnan = 0.463 ± 0.020 mg g−1 d.w., TGCHimalaya = 0.498 ± 0.019 mg g−1 d.w.) in the middle elevation environments, and decreased in both lower elevation (TGCXinjiang = 0.273 ± 0.014 mg g−1 d.w.) and higher elevation (TGCQTP = 0.346 ± 0.021 mg g−1 d.w.) environments, which followed a hump-shaped pattern along the altitudinal gradient. According to the principal component analysis, the 59 investigated landraces were dissected into two clusters, Yunnan-Himalaya environment and Xinjiang-Qinghai Tibetan Plateau environment. Significant correlation was observed between the aboveground biomass and underground biomass (r = 0.653, p < 0.01), leaf length and tuber diameter (r = 0.448, p < 0.01), whereas the TGCs were neither correlated with the aboveground nor the underground biomass. A total of 18 constituents involving ten glucosinolates, three phenylpropanoids, one lignan and four sulfur compounds were characterized based on their MS/MS fragmentation and UV absorption. Gluconasturtiin (7) and glucobrassicanapin (4) were revealed as the main glucosinolates in Turnip. The TGCs in Turnip varied significantly (one sample t (297) = 34.859, P = 0.00) with different climatic zones, of which warm and humid climate [e.g. Yunnan and Himalaya areas (TGCwarm and humid = 0.467 ± 0.014 mg g−1 d.w.)] is profitable for the accumulation of glucosinolates, as compared to the arid and stressful environment [e.g. QTP and Xinjiang areas (TGCarid and stressful = 0.329 ± 0.014 mg g−1 d.w.)]. This investigation suggested that the glucosinolate profiles were closely related to the genes involved in regulating glucosinolate biosynthesis, herbivore pressure and vegetative growth.
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selenium accumulation characteristics and biofortification potentiality in Turnip brassica rapa var rapa supplied with selenite or selenate
Frontiers in Plant Science, 2018Co-Authors: Yonghong Yang, Yongping YangAbstract:Selenium (Se) is an essential trace element for humans. About 70% of the regions in China, including most of the Tibetan Plateau, are faced with Se deficiency problems. Turnip is mainly distributed around the Tibetan Plateau and is one of the few local crops. In the present study, we compared the absorption and translocation differences of Se (Ⅳ) selenite and Se (Ⅵ) selenate in Turnip. The results showed that Se treatment, either by soil addition (0.2–2 mg Se kg-1 dry soil) or by foliar spraying (50–200 mg L-1 Se), could significantly increase the Se concentrations in Turnips, and 0.5 mg Se (Ⅳ) or Se (Ⅵ) kg-1 dry matter in soils could improve the biomasses of Turnips. Moreover, Turnip absorbed significantly more Se (Ⅵ) than Se (Ⅳ) at the same concentration and also transferred much more Se (Ⅵ) from roots to leaves. Based on the Se concentrations, as well as the bioconcentration factors and translocation coefficients, we considered that Turnip might be a potential Se indicator plants. Subsequently, we estimated the daily Se intake for adults based on the Se concentrations in Turnip roots. The results indicated that Se (Ⅳ) should be more suitable as artificial Se fertilizer for Turnips, although the levels found in most samples in this study could cause selenosis to humans. In addition, we also estimated the optimum and maximum Se concentrations for treating Turnips based on the linear relations between Se concentrations in Turnip roots and Se treatment concentrations. The results provided preliminary and useful information about Se biofortification in Turnips.
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Effect of vernalization on tuberization and flowering in the Tibetan Turnip is associated with changes in the expression of FLC homologues
KeAi, 2018Co-Authors: Yan Zheng, Chuntao Wang, Landi Luo, Yuanyuan Liu, Yunqiang Yang, Xiangxiang Kong, Yongping YangAbstract:The Turnip (Brassica rapa var. rapa) is a biennial crop that is planted in late summer/early fall and forms fleshy tubers for food in temperate regions. The harvested tubers then overwinter and are planted again the next spring for flowering and seeds. FLOWERING LOCUS C (FLC) is a MADS-box transcription factor that acts as a major repressor of floral transition by suppressing the flowering promoters FT and SOC1. Here we show that vernalization effectively represses tuber formation and promotes flowering in Tibetan Turnip. We functionally characterized four FLC homologues (BrrFLC1, FLC2, FLC3, and FLC5), and found that BrrFLC2 and BrrFLC1 play a major role in repressing flowering in Turnip and in transgenic Arabidopsis. In contrast, tuber formation was correlated with BrrFLC1 expression in the hypocotyl and was repressed under cold treatment following the quantitative downregulation of BrrFLC1. Grafting experiments of non-vernalized and vernalized Turnips revealed that vernalization independently suppressed tuberization in the tuber or hypocotyl of the rootstock or scion, which occurred in parallel with the reduction in BrrFLC1 activity. Together, our results demonstrate that the Tibetan Turnip is highly responsive to cold exposure, which is associated with the expression levels of BrrFLC genes. Keywords: Tibetan Turnip, Tuberization, Flowering, BrrFLC genes, Vernalizatio
Sarah E Hale - One of the best experts on this subject based on the ideXlab platform.
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can polyethylene passive samplers predict polychlorinated biphenyls pcbs uptake by earthworms and Turnips in a biochar amended soil
Science of The Total Environment, 2019Co-Authors: Ludovica Silvani, Gerard Cornelissen, Sigurbjorg Hjartardottir, Lucie Bielska, Lucia Skulcova, Luca Nizzetto, Sarah E HaleAbstract:A pot experiment was carried out in which aged polychlorinated biphenyls (PCBs) contaminated soil was amended with biochar, and three phases: earthworms, Turnips and polyethylene (PE) passive samplers, were added simultaneously in order to investigate changes in bioavailability of PCB following biochar amendment. Two biochars were used: one made from rice husk in Indonesia using local techniques and the other made from mixed wood shavings using more advanced technology. The biochars were amended at 1 and 4% doses. The overall accumulation of PCBs to the phases followed the order: earthworm lipid > PE > Turnip. The rice husk biochar reduced PCB accumulation to a greater degree than the mixed wood biochar for all phases, however there was no effect of dose for either biochar. Earthworm uptake was reduced between 52% and 91% for rice husk biochar and by 19% to 63% formix wood biochar. Turnip uptake was not significantly reduced by biochar amendment. Phase to soil accumulation factors (PSAF) were around 0.5 for Turnips, approximately 5 for PE and exceeded 100 for earthworms. This study demonstrates that both biochars canbe a sustainable alternative for in situ soil remediation and that PE can be used as tool to simulate the uptake in earthworms and thus remediation effectiveness. (c) 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license.
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Can polyethylene passive samplers predict polychlorinated biphenyls (PCBs) uptake by earthworms and Turnips in a biochar amended soil?
Elsevier, 2019Co-Authors: Ludovica Silvani, Gerard Cornelissen, Sigurbjorg Hjartardottir, Lucie Bielska, Lucia Skulcova, Luca Nizzetto, Sarah E HaleAbstract:A pot experiment was carried out in which aged polychlorinated biphenyls (PCBs) contaminated soil was amended with biochar, and three phases: earthworms, Turnips and polyethylene (PE) passive samplers, were added simultaneously in order to investigate changes in bioavailability of PCB following biochar amendment. Two biochars were used: one made from rice husk in Indonesia using local techniques and the other made from mixed wood shavings using more advanced technology. The biochars were amended at 1 and 4% doses. The overall accumulation of PCBs to the phases followed the order: earthworm lipid > PE > Turnip. The rice husk biochar reduced PCB accumulation to a greater degree than the mixed wood biochar for all phases, however there was no effect of dose for either biochar. Earthworm uptake was reduced between 52% and 91% for rice husk biochar and by 19% to 63% for mix wood biochar. Turnip uptake was not significantly reduced by biochar amendment. Phase to soil accumulation factors (PSAF) were around 0.5 for Turnips, approximately 5 for PE and exceeded 100 for earthworms. This study demonstrates that both biochars can be a sustainable alternative for in situ soil remediation and that PE can be used as tool to simulate the uptake in earthworms and thus remediation effectiveness
Gerard Cornelissen - One of the best experts on this subject based on the ideXlab platform.
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can polyethylene passive samplers predict polychlorinated biphenyls pcbs uptake by earthworms and Turnips in a biochar amended soil
Science of The Total Environment, 2019Co-Authors: Ludovica Silvani, Gerard Cornelissen, Sigurbjorg Hjartardottir, Lucie Bielska, Lucia Skulcova, Luca Nizzetto, Sarah E HaleAbstract:A pot experiment was carried out in which aged polychlorinated biphenyls (PCBs) contaminated soil was amended with biochar, and three phases: earthworms, Turnips and polyethylene (PE) passive samplers, were added simultaneously in order to investigate changes in bioavailability of PCB following biochar amendment. Two biochars were used: one made from rice husk in Indonesia using local techniques and the other made from mixed wood shavings using more advanced technology. The biochars were amended at 1 and 4% doses. The overall accumulation of PCBs to the phases followed the order: earthworm lipid > PE > Turnip. The rice husk biochar reduced PCB accumulation to a greater degree than the mixed wood biochar for all phases, however there was no effect of dose for either biochar. Earthworm uptake was reduced between 52% and 91% for rice husk biochar and by 19% to 63% formix wood biochar. Turnip uptake was not significantly reduced by biochar amendment. Phase to soil accumulation factors (PSAF) were around 0.5 for Turnips, approximately 5 for PE and exceeded 100 for earthworms. This study demonstrates that both biochars canbe a sustainable alternative for in situ soil remediation and that PE can be used as tool to simulate the uptake in earthworms and thus remediation effectiveness. (c) 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license.
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Can polyethylene passive samplers predict polychlorinated biphenyls (PCBs) uptake by earthworms and Turnips in a biochar amended soil?
Elsevier, 2019Co-Authors: Ludovica Silvani, Gerard Cornelissen, Sigurbjorg Hjartardottir, Lucie Bielska, Lucia Skulcova, Luca Nizzetto, Sarah E HaleAbstract:A pot experiment was carried out in which aged polychlorinated biphenyls (PCBs) contaminated soil was amended with biochar, and three phases: earthworms, Turnips and polyethylene (PE) passive samplers, were added simultaneously in order to investigate changes in bioavailability of PCB following biochar amendment. Two biochars were used: one made from rice husk in Indonesia using local techniques and the other made from mixed wood shavings using more advanced technology. The biochars were amended at 1 and 4% doses. The overall accumulation of PCBs to the phases followed the order: earthworm lipid > PE > Turnip. The rice husk biochar reduced PCB accumulation to a greater degree than the mixed wood biochar for all phases, however there was no effect of dose for either biochar. Earthworm uptake was reduced between 52% and 91% for rice husk biochar and by 19% to 63% for mix wood biochar. Turnip uptake was not significantly reduced by biochar amendment. Phase to soil accumulation factors (PSAF) were around 0.5 for Turnips, approximately 5 for PE and exceeded 100 for earthworms. This study demonstrates that both biochars can be a sustainable alternative for in situ soil remediation and that PE can be used as tool to simulate the uptake in earthworms and thus remediation effectiveness
Ludovica Silvani - One of the best experts on this subject based on the ideXlab platform.
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can polyethylene passive samplers predict polychlorinated biphenyls pcbs uptake by earthworms and Turnips in a biochar amended soil
Science of The Total Environment, 2019Co-Authors: Ludovica Silvani, Gerard Cornelissen, Sigurbjorg Hjartardottir, Lucie Bielska, Lucia Skulcova, Luca Nizzetto, Sarah E HaleAbstract:A pot experiment was carried out in which aged polychlorinated biphenyls (PCBs) contaminated soil was amended with biochar, and three phases: earthworms, Turnips and polyethylene (PE) passive samplers, were added simultaneously in order to investigate changes in bioavailability of PCB following biochar amendment. Two biochars were used: one made from rice husk in Indonesia using local techniques and the other made from mixed wood shavings using more advanced technology. The biochars were amended at 1 and 4% doses. The overall accumulation of PCBs to the phases followed the order: earthworm lipid > PE > Turnip. The rice husk biochar reduced PCB accumulation to a greater degree than the mixed wood biochar for all phases, however there was no effect of dose for either biochar. Earthworm uptake was reduced between 52% and 91% for rice husk biochar and by 19% to 63% formix wood biochar. Turnip uptake was not significantly reduced by biochar amendment. Phase to soil accumulation factors (PSAF) were around 0.5 for Turnips, approximately 5 for PE and exceeded 100 for earthworms. This study demonstrates that both biochars canbe a sustainable alternative for in situ soil remediation and that PE can be used as tool to simulate the uptake in earthworms and thus remediation effectiveness. (c) 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license.
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Can polyethylene passive samplers predict polychlorinated biphenyls (PCBs) uptake by earthworms and Turnips in a biochar amended soil?
Elsevier, 2019Co-Authors: Ludovica Silvani, Gerard Cornelissen, Sigurbjorg Hjartardottir, Lucie Bielska, Lucia Skulcova, Luca Nizzetto, Sarah E HaleAbstract:A pot experiment was carried out in which aged polychlorinated biphenyls (PCBs) contaminated soil was amended with biochar, and three phases: earthworms, Turnips and polyethylene (PE) passive samplers, were added simultaneously in order to investigate changes in bioavailability of PCB following biochar amendment. Two biochars were used: one made from rice husk in Indonesia using local techniques and the other made from mixed wood shavings using more advanced technology. The biochars were amended at 1 and 4% doses. The overall accumulation of PCBs to the phases followed the order: earthworm lipid > PE > Turnip. The rice husk biochar reduced PCB accumulation to a greater degree than the mixed wood biochar for all phases, however there was no effect of dose for either biochar. Earthworm uptake was reduced between 52% and 91% for rice husk biochar and by 19% to 63% for mix wood biochar. Turnip uptake was not significantly reduced by biochar amendment. Phase to soil accumulation factors (PSAF) were around 0.5 for Turnips, approximately 5 for PE and exceeded 100 for earthworms. This study demonstrates that both biochars can be a sustainable alternative for in situ soil remediation and that PE can be used as tool to simulate the uptake in earthworms and thus remediation effectiveness
Yunqiang Yang - One of the best experts on this subject based on the ideXlab platform.
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brrice1 1 is associated with putrescine synthesis through regulation of the arginine decarboxylase gene in freezing tolerance of Turnip brassica rapa var rapa
BMC Plant Biology, 2020Co-Authors: Xin Yin, Yongping Yang, Yunqiang Yang, Danni Yang, Yanling YueAbstract:Background In the agricultural areas of Qinghai-Tibet Plateau, temperature varies widely from day to night during the growing season, which makes the extreme temperature become one of the limiting factors of crop yield. Turnip (Brassica rapa var. rapa) is a traditional crop of Tibet grown in the Tibet Plateau, but its molecular and metabolic mechanisms of freezing tolerance are unclear. Results Here, based on the changes in transcriptional and metabolic levels of Tibetan Turnip under freezing treatment, the expression of the arginine decarboxylase gene BrrADC2.2 exhibited an accumulative pattern in accordance with putrescine content. Moreover, we demonstrated that BrrICE1.1 (Inducer of CBF Expression 1) could directly bind to the BrrADC2.2 promoter, activating BrrADC2.2 to promote the accumulation of putrescine, which was verified by RNAi and overexpression analyses for both BrrADC2.2 and BrrICE1.1 using transgenic hair root. The function of putrescine in Turnip was further analyzed by exogenous application putrescine and its inhibitor DL-α-(Difluoromethyl) arginine (DFMA) under freezing tolerance. In addition, the BrrICE1.1 was found to be involved in the ICE1-CBF pathway to increase the freezing stress of Turnip. Conclusions BrrICE1.1 could bind the promoter of BrrADC2.2 or CBFs to participate in freezing tolerance of Turnip by transcriptomics and targeted metabolomics analyses. This study revealed the regulatory network of the freezing tolerance process in Turnip and increased our understanding of the plateau crops response to extreme environments in Tibet.
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brrice1 1 is associated with putrescine synthesis through regulation of the arginine decarboxylase gene in freezing tolerance of Turnip brassica rapa var rapa
BMC Plant Biology, 2020Co-Authors: Xin Yin, Yongping Yang, Yunqiang Yang, Danni Yang, Yanling YueAbstract:In the agricultural areas of Qinghai-Tibet Plateau, temperature varies widely from day to night during the growing season, which makes the extreme temperature become one of the limiting factors of crop yield. Turnip (Brassica rapa var. rapa) is a traditional crop of Tibet grown in the Tibet Plateau, but its molecular and metabolic mechanisms of freezing tolerance are unclear. Here, based on the changes in transcriptional and metabolic levels of Tibetan Turnip under freezing treatment, the expression of the arginine decarboxylase gene BrrADC2.2 exhibited an accumulative pattern in accordance with putrescine content. Moreover, we demonstrated that BrrICE1.1 (Inducer of CBF Expression 1) could directly bind to the BrrADC2.2 promoter, activating BrrADC2.2 to promote the accumulation of putrescine, which was verified by RNAi and overexpression analyses for both BrrADC2.2 and BrrICE1.1 using transgenic hair root. The function of putrescine in Turnip was further analyzed by exogenous application putrescine and its inhibitor DL-α-(Difluoromethyl) arginine (DFMA) under freezing tolerance. In addition, the BrrICE1.1 was found to be involved in the ICE1-CBF pathway to increase the freezing stress of Turnip. BrrICE1.1 could bind the promoter of BrrADC2.2 or CBFs to participate in freezing tolerance of Turnip by transcriptomics and targeted metabolomics analyses. This study revealed the regulatory network of the freezing tolerance process in Turnip and increased our understanding of the plateau crops response to extreme environments in Tibet.
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Effect of vernalization on tuberization and flowering in the Tibetan Turnip is associated with changes in the expression of FLC homologues
KeAi, 2018Co-Authors: Yan Zheng, Chuntao Wang, Landi Luo, Yuanyuan Liu, Yunqiang Yang, Xiangxiang Kong, Yongping YangAbstract:The Turnip (Brassica rapa var. rapa) is a biennial crop that is planted in late summer/early fall and forms fleshy tubers for food in temperate regions. The harvested tubers then overwinter and are planted again the next spring for flowering and seeds. FLOWERING LOCUS C (FLC) is a MADS-box transcription factor that acts as a major repressor of floral transition by suppressing the flowering promoters FT and SOC1. Here we show that vernalization effectively represses tuber formation and promotes flowering in Tibetan Turnip. We functionally characterized four FLC homologues (BrrFLC1, FLC2, FLC3, and FLC5), and found that BrrFLC2 and BrrFLC1 play a major role in repressing flowering in Turnip and in transgenic Arabidopsis. In contrast, tuber formation was correlated with BrrFLC1 expression in the hypocotyl and was repressed under cold treatment following the quantitative downregulation of BrrFLC1. Grafting experiments of non-vernalized and vernalized Turnips revealed that vernalization independently suppressed tuberization in the tuber or hypocotyl of the rootstock or scion, which occurred in parallel with the reduction in BrrFLC1 activity. Together, our results demonstrate that the Tibetan Turnip is highly responsive to cold exposure, which is associated with the expression levels of BrrFLC genes. Keywords: Tibetan Turnip, Tuberization, Flowering, BrrFLC genes, Vernalizatio
