Tymovirus

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R. Koenig - One of the best experts on this subject based on the ideXlab platform.

  • the complete genome sequences of a peruvian and a colombian isolate of andean potato latent virus and partial sequences of further isolates suggest the existence of two distinct potato infecting Tymovirus species
    Virus Research, 2013
    Co-Authors: Jan Kreuze, R. Koenig, Joao De Souza, Heinrich Josef Vetten, Giovanna Muller, Betty Flores, Heiko Ziebell, Wilmer J Cuellar
    Abstract:

    The complete genomic RNA sequences of the Tymovirus isolates Hu and Col from potato which originally had been considered to be strains of the same virus species, i.e. Andean potato latent virus (APLV), were determined by siRNA sequencing and assembly, and found to share only c. 65% nt sequence identity. This result together with those of serological tests and comparisons of the coat protein gene sequences of additional Tymovirus isolates from potato suggest that the species Andean potato latent virus should be subdivided into two species, i.e. APLV and Andean potato mild mosaic virus (APMMV). Primers were designed for the broad specificity detection of both viruses.

  • Molecular characterization of isolates of anagyris vein yellowing virus, plantago mottle virus and scrophularia mottle virus – comparison of various approaches for Tymovirus classification
    Archives of Virology, 2005
    Co-Authors: R. Koenig, D.-e. Lesemann, C. W. A. Pleij, S. Loss, H. J. Vetten
    Abstract:

    The complete nucleotide sequences were determined for the genomic RNAs of three Tymoviruses, i.e. isolates of anagyris vein yellowing virus (AVYV), plantago mottle virus (PlMoV) and scrophularia mottle virus (SrMV) which are all serologically closely related to ononis yellow mosaic virus (ibid) and to Nemesia ring necrosis virus (NeRNV), a recently described recombinant virus which is widely spread in commercially grown ornamental plant species belonging to the Scrophulariaceae . Total nucleotide and coat protein amino acid sequence identities revealed similar groupings in the genus Tymovirus as serological studies did. The latter, however, tended to suggest much closer relationships than the molecular data and may fail to recognise the distinctiveness of new Tymovirus species. The usefulness of various species demarcation criteria for the classification of Tymoviruses is discussed.

  • petunia vein banding virus characterization of a new Tymovirus from petunia hybrida
    Plant Disease, 2000
    Co-Authors: M. A. V. Alexandre, L. M. L. Duarte, E. B. Rivas, C. M. Chagas, Maria Mércia Barradas, R. Koenig
    Abstract:

    Petunia plants from a nursery in the State of Rio Grande do Sul, Brazil, showed pronounced vein banding and contained isometric particles with diameters of approximately 45 and 30 nm. The larger ones apparently represent a caulimovirus, while the smaller ones, which included both empty shells and full particles, were identified as those of a new Tymovirus for which we propose the name Petunia vein banding virus (PetVBV). Originally, PetVBV was transmitted only with difficulty to healthy petunia plants. However, from an experimentally infected petu-nia, it was later readily transmitted also to Nicotiana benthamiana and Nicandra physalodes, but not to other species in the Solanaceae or other plant families. It produces cytopathic effects typical for Tymovirus infections. Its coat protein shows approximately 65% amino acid sequence identity with those of Eggplant mosaic and Andean potato latent viruses, to which it is also serologically more closely related than to any other Tymoviruses.

  • Petunia vein banding virus: Characterization of a New Tymovirus from Petunia × hybrida
    Plant disease, 2000
    Co-Authors: M. A. V. Alexandre, L. M. L. Duarte, E. B. Rivas, C. M. Chagas, Maria Mércia Barradas, R. Koenig
    Abstract:

    Petunia plants from a nursery in the State of Rio Grande do Sul, Brazil, showed pronounced vein banding and contained isometric particles with diameters of approximately 45 and 30 nm. The larger ones apparently represent a caulimovirus, while the smaller ones, which included both empty shells and full particles, were identified as those of a new Tymovirus for which we propose the name Petunia vein banding virus (PetVBV). Originally, PetVBV was transmitted only with difficulty to healthy petunia plants. However, from an experimentally infected petu-nia, it was later readily transmitted also to Nicotiana benthamiana and Nicandra physalodes, but not to other species in the Solanaceae or other plant families. It produces cytopathic effects typical for Tymovirus infections. Its coat protein shows approximately 65% amino acid sequence identity with those of Eggplant mosaic and Andean potato latent viruses, to which it is also serologically more closely related than to any other Tymoviruses.

H. J. Vetten - One of the best experts on this subject based on the ideXlab platform.

A. J. Gibbs - One of the best experts on this subject based on the ideXlab platform.

  • A Tymovirus from Calopogonium mucunoides in Malaysia is not clitoria yellow vein Tymovirus
    Archives of Virology, 1997
    Co-Authors: A. J. Gibbs, A. M. Mackenzie, N. Abdul-samad
    Abstract:

    A Tymovirus isolated from Malaysian crops of Calopogonium mucunoides has been shown to have virions that are serologically indistinguishable from those of clitoria yellow vein Tymovirus. We have sequenced the virion protein (VP) gene of the virus and have found that although it is a member of the cluster that includes CYVV, it is the most distinct member of that cluster (

  • Genetic variation in populations of kennedya yellow mosaic Tymovirus
    Archives of Virology, 1996
    Co-Authors: M. L. Skotnicki, A. M. Mackenzie, A. J. Gibbs
    Abstract:

    Kennedya yellow mosaic Tymovirus (KYMV) occurs along the eastern Australian seaboard in the perennial legumes Desmodium triflorum and D. scorpiurus in the north, and Kennedya rubicunda in the south. The genetic variation of more than 100 isolates of KYMV, most of them from the north, has been studied using an RNA hybrid mismatch polymorphism (RHMP) method. The method clearly separated the isolates into two groups; all the northern Desmodium isolates formed one group and all the Kennedya isolates from the south another. These sub-populations were themselves variable and the Desmodium population alone was more variable than that of the related turnip yellow mosaic Tymovirus in the Kosciusko alpine area.

  • Infectious eggplant mosaic Tymovirus and ononis yellow mosaic Tymovirus from cloned cDNA
    Archives of Virology, 1993
    Co-Authors: M. L. Skotnicki, A. M. Mackenzie, S. -w. Ding, A. J. Gibbs
    Abstract:

    Eggplant mosaic virus (EMV) and ononis yellow mosaic virus (OYMV) are two Tymoviruses that have ssRNA genomes of about 6.2 kb and 6.3 kb, and which infect solanaceous and leguminous hosts, respectively. Full-length cDNA clones of these viruses were constructed with a T 7 promoter adjacent to the 5′ terminus of the DNA copy of the viral genome, and with unique restriction endonuclease sites at the 3′ terminus. This allowed RNA to be transcribed from the DNA encoding the genome. The transcript RNA was infectious when inoculated to Nicotiana glutinosa (for EMV) and Pisum sativum (for OYMV). These clones, together with clones of turnip yellow mosaic Tymovirus, which infects brassicas, have been used to construct hybrids in which the virion protein gene was exchanged between EMV or OYMV and turnip yellow mosaic virus. These and other hybrids are being used to investigate the molecular basis for host range differences in Tymoviruses.

A. M. Mackenzie - One of the best experts on this subject based on the ideXlab platform.

  • A Tymovirus from Calopogonium mucunoides in Malaysia is not clitoria yellow vein Tymovirus
    Archives of Virology, 1997
    Co-Authors: A. J. Gibbs, A. M. Mackenzie, N. Abdul-samad
    Abstract:

    A Tymovirus isolated from Malaysian crops of Calopogonium mucunoides has been shown to have virions that are serologically indistinguishable from those of clitoria yellow vein Tymovirus. We have sequenced the virion protein (VP) gene of the virus and have found that although it is a member of the cluster that includes CYVV, it is the most distinct member of that cluster (

  • A Tymovirus from Calopogonium mucunoides in Malaysia is not clitoria yellow vein Tymovirus
    Archives of virology, 1997
    Co-Authors: Adrian Gibbs, A. M. Mackenzie, N. Abdul-samad
    Abstract:

    A Tymovirus isolated from Malaysian crops of Calopogonium mucunoides has been shown to have virions that are serologically indistinguishable from those of clitoria yellow vein Tymovirus. We have sequenced the virion protein (VP) gene of the virus and have found that although it is a member of the cluster that includes CYVV, it is the most distinct member of that cluster (< 62% sequence identity with all the others), and is clearly a separate species, which we propose should be named calopogonium yellow vein virus. Most of the serological specificity of the virions of Tymoviruses seems to reside in the C-terminal hexapeptide of the virion protein

  • Genetic variation in populations of kennedya yellow mosaic Tymovirus
    Archives of Virology, 1996
    Co-Authors: M. L. Skotnicki, A. M. Mackenzie, A. J. Gibbs
    Abstract:

    Kennedya yellow mosaic Tymovirus (KYMV) occurs along the eastern Australian seaboard in the perennial legumes Desmodium triflorum and D. scorpiurus in the north, and Kennedya rubicunda in the south. The genetic variation of more than 100 isolates of KYMV, most of them from the north, has been studied using an RNA hybrid mismatch polymorphism (RHMP) method. The method clearly separated the isolates into two groups; all the northern Desmodium isolates formed one group and all the Kennedya isolates from the south another. These sub-populations were themselves variable and the Desmodium population alone was more variable than that of the related turnip yellow mosaic Tymovirus in the Kosciusko alpine area.

  • Infectious eggplant mosaic Tymovirus and ononis yellow mosaic Tymovirus from cloned cDNA
    Archives of Virology, 1993
    Co-Authors: M. L. Skotnicki, A. M. Mackenzie, S. -w. Ding, A. J. Gibbs
    Abstract:

    Eggplant mosaic virus (EMV) and ononis yellow mosaic virus (OYMV) are two Tymoviruses that have ssRNA genomes of about 6.2 kb and 6.3 kb, and which infect solanaceous and leguminous hosts, respectively. Full-length cDNA clones of these viruses were constructed with a T 7 promoter adjacent to the 5′ terminus of the DNA copy of the viral genome, and with unique restriction endonuclease sites at the 3′ terminus. This allowed RNA to be transcribed from the DNA encoding the genome. The transcript RNA was infectious when inoculated to Nicotiana glutinosa (for EMV) and Pisum sativum (for OYMV). These clones, together with clones of turnip yellow mosaic Tymovirus, which infects brassicas, have been used to construct hybrids in which the virion protein gene was exchanged between EMV or OYMV and turnip yellow mosaic virus. These and other hybrids are being used to investigate the molecular basis for host range differences in Tymoviruses.

N. Abdul-samad - One of the best experts on this subject based on the ideXlab platform.

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