The Experts below are selected from a list of 81 Experts worldwide ranked by ideXlab platform
Shioko Kimura - One of the best experts on this subject based on the ideXlab platform.
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development of thyroid gland and Ultimobranchial Body cyst is independent of p63
Laboratory Investigation, 2011Co-Authors: Takashi Ozaki, Kunio Nagashima, Takashi Kusakabe, Kennichi Kakudo, Shioko KimuraAbstract:The Ultimobranchial Body (UBB) and thyroid primordium are the origins of the thyroid gland that fuse around embryonic day 14.5 of mouse gestation, ultimately giving rise to calcitonin-producing C cells and thyroglobulin-producing follicular cells, respectively. A homeodomain transcription factor NKX2-1 is expressed both in the UBB and the thyroid primordium, and is critical for development of the thyroid gland. In this study, the role of p63 in development of UBB and the thyroid gland was analyzed by histological, immunohistochemical, and electron microscopic analyses using mice with various combinations of Nkx2-1 and p63 wild-type, heterozygous, and null alleles. In the absence of p63, a normal thyroid gland develops, as revealed by expression of thyroglobulin and calcitonin, thus showing that p63 is not required for thyroid development. However, in mice carrying the Nkx2-1-null allele, the UBB remains as a cystic vesicular structure and/or in nested patterns consisting of p63-positive cells surrounding the vesicle and undifferentiated immature cells with occasional cilia lying inside. The cystic UBB was present even in the Nkx2-1;p63 double-null mice. The structure and p63 expression pattern of the UBB cyst strikingly resemble the solid cell nest. These results show that in the absence of NKX2-1, UBB becomes cystic independent of p63, which is likely the origin of SCN.
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origin of the Ultimobranchial Body cyst t ebp nkx2 1 expression is required for development and fusion of the Ultimobranchial Body to the thyroid
Developmental Dynamics, 2006Co-Authors: Takashi Kusakabe, Nobuo Hoshi, Shioko KimuraAbstract:The Ultimobranchial Body (UBB) is an outpocketing of the fourth pharyngeal pouch that fuses with the thyroid diverticulum, giving rise to calcitonin-producing C-cells. In this study, we demonstrate that the UBB is composed of two types of cells: one expressing T/ebp/Nkx2.1 and the other expressing p63. The former cell type, accounting for a majority of the UBB, requires T/ebp/Nkx2.1 for their survival. In contrast, the p63-positive cells, even in the absence of T/ebp/Nkx2.1 expression, can proliferate and give rise to a vesicular structure that is lined by a monolayer of p63-negative cells, surrounded by a cluster and/or single layer of p63-positive cells, displaying the basal/stem cell phenotype. T/ebp/Nkx2.1 haploinsufficiency causes abnormal fusion of the UBB with the thyroid diverticulum, which stays as a cluster of C-cells around the vesicular structure, similar to the one observed in mice null for T/ebp/Nkx2.1 expression. These results demonstrate that T/ebp/Nkx2.1 plays a role in the survival of UBB cells, their dissemination into the thyroid diverticulum, and the formation of UBB-derived vesicular structure.
Moreno-gomez Freddy - One of the best experts on this subject based on the ideXlab platform.
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¿Cresta Neural o Endodermo? Conceptos divergentes sobre el origen embrionario de las células C de la tiroides
Pontificia Universidad Javeriana Cali, 2017Co-Authors: Medina Sebastián, Moreno-gomez FreddyAbstract:Las células C (o parafoliculares), son una población pequeña de células ubicadas en el parénquima de la glándula tiroides y están encargadas de sintetizar calcitonina. Debido a que recientemente se ha planteado la posibilidad que el origen de las células C de los mamíferos sea a partir de las células endodérmicas del cuerpo ultimobranquial y no de una oleada migratoria de las células de la cresta neural (CCN), se ha generado controversia que se remonta desde 1930 hasta la evidencia generada por estudios con técnicas mas sofisticados de inmunohistoquímica y rastreo genético llevados a cabo desde el 2010. El objetivo de esta revisión de tema es presentar la información disponible en la literatura especializada y contrastarla con la información que se encuentra en los libros académicos de biología del desarrollo e histología, referente al origen embrionario de las células C de la tiroides en los mamíferos, incluídos los seres humanos. De acuerdo a los estudios de rastreo genético en modelos murinos se ha identificado que las células C derivan de las células endodérmicas del cuerpo últimobranquial de la cuarta bolsa faríngea, y no de las CCN como concluyó en los primeros estudios hace poco mas de 70 años. A pesar de esto, las CCN son fundamentales para el proceso de formación de dichas células. Abstract:C-cells (or parafollicular cells) are a small population of cells located in the parenchyma of the thyroid gland and are responsible for synthesizing calcitonin. Because it has recently been suggested that the origin of mammalian C-cells is from the endodermal cells of the Ultimobranchial Body and not from a migratory wave of neural crest cells (NCC), controversy has been generated that goes back to 1930 from the evidence generated by studies with more sophisticated techniques of immunohistochemistry and genetic tracking carried out since 2010. The aim of this review is to present the available information and to compare it with the information that is found in the academic books of biology of the development and histology, referring to the embryonic origin of the C cells of the thyroid in mammals, including humans. According to genetic screening studies in murine models, it has been identified that C cells derive from the endodermal cells of the latebranchial Body of the fourth pharyngeal pouch, and not from NCC as concluded in the earlier studies a little more of 70 years. Despite this, the NCC are fundamental for the process of formation of these cells.Key words: Developmental biology, pharyngeal apparatus, pharyngeal pouches, Ultimobranchial Body, neural crest cells, C-cells
Takashi Kusakabe - One of the best experts on this subject based on the ideXlab platform.
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development of thyroid gland and Ultimobranchial Body cyst is independent of p63
Laboratory Investigation, 2011Co-Authors: Takashi Ozaki, Kunio Nagashima, Takashi Kusakabe, Kennichi Kakudo, Shioko KimuraAbstract:The Ultimobranchial Body (UBB) and thyroid primordium are the origins of the thyroid gland that fuse around embryonic day 14.5 of mouse gestation, ultimately giving rise to calcitonin-producing C cells and thyroglobulin-producing follicular cells, respectively. A homeodomain transcription factor NKX2-1 is expressed both in the UBB and the thyroid primordium, and is critical for development of the thyroid gland. In this study, the role of p63 in development of UBB and the thyroid gland was analyzed by histological, immunohistochemical, and electron microscopic analyses using mice with various combinations of Nkx2-1 and p63 wild-type, heterozygous, and null alleles. In the absence of p63, a normal thyroid gland develops, as revealed by expression of thyroglobulin and calcitonin, thus showing that p63 is not required for thyroid development. However, in mice carrying the Nkx2-1-null allele, the UBB remains as a cystic vesicular structure and/or in nested patterns consisting of p63-positive cells surrounding the vesicle and undifferentiated immature cells with occasional cilia lying inside. The cystic UBB was present even in the Nkx2-1;p63 double-null mice. The structure and p63 expression pattern of the UBB cyst strikingly resemble the solid cell nest. These results show that in the absence of NKX2-1, UBB becomes cystic independent of p63, which is likely the origin of SCN.
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origin of the Ultimobranchial Body cyst t ebp nkx2 1 expression is required for development and fusion of the Ultimobranchial Body to the thyroid
Developmental Dynamics, 2006Co-Authors: Takashi Kusakabe, Nobuo Hoshi, Shioko KimuraAbstract:The Ultimobranchial Body (UBB) is an outpocketing of the fourth pharyngeal pouch that fuses with the thyroid diverticulum, giving rise to calcitonin-producing C-cells. In this study, we demonstrate that the UBB is composed of two types of cells: one expressing T/ebp/Nkx2.1 and the other expressing p63. The former cell type, accounting for a majority of the UBB, requires T/ebp/Nkx2.1 for their survival. In contrast, the p63-positive cells, even in the absence of T/ebp/Nkx2.1 expression, can proliferate and give rise to a vesicular structure that is lined by a monolayer of p63-negative cells, surrounded by a cluster and/or single layer of p63-positive cells, displaying the basal/stem cell phenotype. T/ebp/Nkx2.1 haploinsufficiency causes abnormal fusion of the UBB with the thyroid diverticulum, which stays as a cluster of C-cells around the vesicular structure, similar to the one observed in mice null for T/ebp/Nkx2.1 expression. These results demonstrate that T/ebp/Nkx2.1 plays a role in the survival of UBB cells, their dissemination into the thyroid diverticulum, and the formation of UBB-derived vesicular structure.
Ozgur Mete - One of the best experts on this subject based on the ideXlab platform.
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positivity for gata3 and ttf 1 spt24 and negativity for monoclonal pax8 expand the biomarker profile of the solid cell nests of the thyroid gland
Endocrine Pathology, 2018Co-Authors: Hasan Gucer, Ozgur MeteAbstract:Solid cell nests (SCNs) are usually distinguished on conventional HE however, the morphological heterogeneity in SCNs and hyperplasia of these Ultimobranchial Body remnants can mimic other diagnostic entities including but not limited to papillary microcarcinoma. In order to confirm the thyroid follicular epithelial origin and exclude the possibility of SCNs, most diagnosticians use immunohistochemical biomarkers of thyroid follicular epithelial cells and/or those of SCNs. While the expression profile of monoclonal PAX8 has not been reported previously in SCNs, the status of TTF-1 expression using the 8G7G3/1 clone has been inconsistent among several studies. Given the potential diagnostic pitfalls, this series investigated the expression profile of GATA3, monoclonal PAX8, and TTF-1 (SPT24), along with p63, p40, monoclonal calcitonin, monoclonal CEA, and HBME-1 in a tissue microarray (TMA) of 56 SCNs. SCNs were all diffusely and strongly positive for TTF-1 (SPT24), p63, and p40, and were negative for monoclonal PAX8 and calcitonin. Positivity for GATA3 and monoclonal CEA was identified in 41 (73.2%) and 36 (64.3%) of SCNs. In addition, 18 (32.1%) SCNs displayed HBME-1 reactivity. These findings expand the immunohistochemical correlates of SCNs by demonstrating positivity for GATA3 and TTF-1 (SPT24), and negativity for monoclonal PAX8. The identification of monoclonal CEA expression and HBME-1 in SCNs also underscores the limitations of these select biomarkers in the distinction of C cell proliferations and papillary microcarcinoma, respectively. The findings of this series also suggest that positivity for TTF-1 (SPT24) alone should not be used to confirm the thyroid follicular epithelial origin. Therefore, the combined use of TTF-1 (SPT24) and monoclonal PAX8 in association with p63 or p40 provides an accurate distinction of SCNs.
Medina Sebastián - One of the best experts on this subject based on the ideXlab platform.
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¿Cresta Neural o Endodermo? Conceptos divergentes sobre el origen embrionario de las células C de la tiroides
Pontificia Universidad Javeriana Cali, 2017Co-Authors: Medina Sebastián, Moreno-gomez FreddyAbstract:Las células C (o parafoliculares), son una población pequeña de células ubicadas en el parénquima de la glándula tiroides y están encargadas de sintetizar calcitonina. Debido a que recientemente se ha planteado la posibilidad que el origen de las células C de los mamíferos sea a partir de las células endodérmicas del cuerpo ultimobranquial y no de una oleada migratoria de las células de la cresta neural (CCN), se ha generado controversia que se remonta desde 1930 hasta la evidencia generada por estudios con técnicas mas sofisticados de inmunohistoquímica y rastreo genético llevados a cabo desde el 2010. El objetivo de esta revisión de tema es presentar la información disponible en la literatura especializada y contrastarla con la información que se encuentra en los libros académicos de biología del desarrollo e histología, referente al origen embrionario de las células C de la tiroides en los mamíferos, incluídos los seres humanos. De acuerdo a los estudios de rastreo genético en modelos murinos se ha identificado que las células C derivan de las células endodérmicas del cuerpo últimobranquial de la cuarta bolsa faríngea, y no de las CCN como concluyó en los primeros estudios hace poco mas de 70 años. A pesar de esto, las CCN son fundamentales para el proceso de formación de dichas células. Abstract:C-cells (or parafollicular cells) are a small population of cells located in the parenchyma of the thyroid gland and are responsible for synthesizing calcitonin. Because it has recently been suggested that the origin of mammalian C-cells is from the endodermal cells of the Ultimobranchial Body and not from a migratory wave of neural crest cells (NCC), controversy has been generated that goes back to 1930 from the evidence generated by studies with more sophisticated techniques of immunohistochemistry and genetic tracking carried out since 2010. The aim of this review is to present the available information and to compare it with the information that is found in the academic books of biology of the development and histology, referring to the embryonic origin of the C cells of the thyroid in mammals, including humans. According to genetic screening studies in murine models, it has been identified that C cells derive from the endodermal cells of the latebranchial Body of the fourth pharyngeal pouch, and not from NCC as concluded in the earlier studies a little more of 70 years. Despite this, the NCC are fundamental for the process of formation of these cells.Key words: Developmental biology, pharyngeal apparatus, pharyngeal pouches, Ultimobranchial Body, neural crest cells, C-cells
