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Paul N Durrington - One of the best experts on this subject based on the ideXlab platform.
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use of the tlx Ultracentrifuge for the isolation of different density lipoproteins and effects of freeze thawing of human plasma before ultracentrifugation
Clinical Chemistry and Laboratory Medicine, 2008Co-Authors: Valentine Charltonmenys, Jelena Chobotova, Paul N DurringtonAbstract:BACKGROUND: Isolation of different density lipoproteins by ultracentrifugation can require lengthy centrifugation times and freeze/thawing of plasma may influence recovery. METHODS: We isolated a range of lipoproteins using a preparative Ultracentrifuge and the TLX micro-Ultracentrifuge and determined the effect of freeze/thawing of plasma beforehand. RESULTS: In fresh plasma, there was no significant difference in results for small-dense low-density lipoprotein apolipoprotein B (LDL apoB) (density >1.044 g/mL) or cholesterol at density >1.006 g/mL. Freeze/thawing had no effect on closely correlated results for small-dense LDL apoB (r=0.85; p<0.0001) or high-density lipoprotein (r=0.93; p<0.0001). CONCLUSIONS: The TLX micro-Ultracentrifuge is a reliable alternative to the preparative Ultracentrifuge and freeze/thawing has only a small effect on small-dense LDL apoB or high-density lipoprotein cholesterol.
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Use of the TLX Ultracentrifuge for the isolation of different density lipoproteins and effects of freeze/thawing of human plasma before ultracentrifugation.
Clinical chemistry and laboratory medicine, 2008Co-Authors: Valentine Charlton-menys, Jelena Chobotova, Paul N DurringtonAbstract:BACKGROUND: Isolation of different density lipoproteins by ultracentrifugation can require lengthy centrifugation times and freeze/thawing of plasma may influence recovery. METHODS: We isolated a range of lipoproteins using a preparative Ultracentrifuge and the TLX micro-Ultracentrifuge and determined the effect of freeze/thawing of plasma beforehand. RESULTS: In fresh plasma, there was no significant difference in results for small-dense low-density lipoprotein apolipoprotein B (LDL apoB) (density >1.044 g/mL) or cholesterol at density >1.006 g/mL. Freeze/thawing had no effect on closely correlated results for small-dense LDL apoB (r=0.85; p
Stephen E Harding - One of the best experts on this subject based on the ideXlab platform.
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recent advances in the analysis of macromolecular interactions using the matrix free method of sedimentation in the analytical Ultracentrifuge
Biology, 2015Co-Authors: Stephen E Harding, Richard B Gillis, Fahad M Almutairi, Tayyibe Erten, M S Kok, Gary G AdamsAbstract:Sedimentation in the analytical Ultracentrifuge is a matrix free solution technique with no immobilisation, columns, or membranes required and can be used to study self-association and complex or “hetero”-interactions, stoichiometry, reversibility and interaction strength of a wide variety of macromolecular types and across a very large dynamic range (dissociation constants from 10−12 M to 10−1 M). We extend an earlier review specifically highlighting advances in sedimentation velocity and sedimentation equilibrium in the analytical Ultracentrifuge applied to protein interactions and mucoadhesion and to review recent applications in protein self-association (tetanus toxoid, agrin), protein-like carbohydrate association (aminocelluloses), carbohydrate-protein interactions (polysaccharide-gliadin), nucleic-acid protein (G-duplexes), nucleic acid-carbohydrate (DNA-chitosan) and finally carbohydrate-carbohydrate (xanthan-chitosan and a ternary polysaccharide complex) interactions.
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The analytical Ultracentrifuge spins again
TrAC Trends in Analytical Chemistry, 1994Co-Authors: Stephen E HardingAbstract:Abstract Since its inception by T. Svedberg and co-workers in the 1920's the technique of analytical ultracentrifugation has undergone an extraordinary evolution, peaking in popularity in the 1950's and 1960's followed by a steady decline until the last few years with a renewal of interest culminating in the launch of a new commercially available instrument. The technique is worthy of consideration by any analytical chemist interested in the characterisation of the physical behaviour of macromolecules in solution. This article attempts to indicate the breadth of the range of different types of measurements on both bio- and synthetic macromolecular systems that are now possible with the analytical Ultracentrifuge
Allen P Minton - One of the best experts on this subject based on the ideXlab platform.
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simulation of the time course of macromolecular separations in an Ultracentrifuge i formation of a cesium chloride density gradient at 25 c
Biophysical Chemistry, 1992Co-Authors: Allen P MintonAbstract:A method is described for rapid numerical simulation of the time course of the formation of a density gradient of CsCl in an Ultracentrifuge at 25 degrees C. Results of simulations compare well with those of experiments carried out in analytical and preparative Ultracentrifuges.
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Simulation of the time course of macromolecular separations in an Ultracentrifuge. I. Formation of a cesium chloride density gradient at 25°C
Biophysical chemistry, 1992Co-Authors: Allen P MintonAbstract:A method is described for rapid numerical simulation of the time course of the formation of a density gradient of CsCl in an Ultracentrifuge at 25 degrees C. Results of simulations compare well with those of experiments carried out in analytical and preparative Ultracentrifuges.
Jelena Chobotova - One of the best experts on this subject based on the ideXlab platform.
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use of the tlx Ultracentrifuge for the isolation of different density lipoproteins and effects of freeze thawing of human plasma before ultracentrifugation
Clinical Chemistry and Laboratory Medicine, 2008Co-Authors: Valentine Charltonmenys, Jelena Chobotova, Paul N DurringtonAbstract:BACKGROUND: Isolation of different density lipoproteins by ultracentrifugation can require lengthy centrifugation times and freeze/thawing of plasma may influence recovery. METHODS: We isolated a range of lipoproteins using a preparative Ultracentrifuge and the TLX micro-Ultracentrifuge and determined the effect of freeze/thawing of plasma beforehand. RESULTS: In fresh plasma, there was no significant difference in results for small-dense low-density lipoprotein apolipoprotein B (LDL apoB) (density >1.044 g/mL) or cholesterol at density >1.006 g/mL. Freeze/thawing had no effect on closely correlated results for small-dense LDL apoB (r=0.85; p<0.0001) or high-density lipoprotein (r=0.93; p<0.0001). CONCLUSIONS: The TLX micro-Ultracentrifuge is a reliable alternative to the preparative Ultracentrifuge and freeze/thawing has only a small effect on small-dense LDL apoB or high-density lipoprotein cholesterol.
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Use of the TLX Ultracentrifuge for the isolation of different density lipoproteins and effects of freeze/thawing of human plasma before ultracentrifugation.
Clinical chemistry and laboratory medicine, 2008Co-Authors: Valentine Charlton-menys, Jelena Chobotova, Paul N DurringtonAbstract:BACKGROUND: Isolation of different density lipoproteins by ultracentrifugation can require lengthy centrifugation times and freeze/thawing of plasma may influence recovery. METHODS: We isolated a range of lipoproteins using a preparative Ultracentrifuge and the TLX micro-Ultracentrifuge and determined the effect of freeze/thawing of plasma beforehand. RESULTS: In fresh plasma, there was no significant difference in results for small-dense low-density lipoprotein apolipoprotein B (LDL apoB) (density >1.044 g/mL) or cholesterol at density >1.006 g/mL. Freeze/thawing had no effect on closely correlated results for small-dense LDL apoB (r=0.85; p
Gary G Adams - One of the best experts on this subject based on the ideXlab platform.
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recent advances in the analysis of macromolecular interactions using the matrix free method of sedimentation in the analytical Ultracentrifuge
Biology, 2015Co-Authors: Stephen E Harding, Richard B Gillis, Fahad M Almutairi, Tayyibe Erten, M S Kok, Gary G AdamsAbstract:Sedimentation in the analytical Ultracentrifuge is a matrix free solution technique with no immobilisation, columns, or membranes required and can be used to study self-association and complex or “hetero”-interactions, stoichiometry, reversibility and interaction strength of a wide variety of macromolecular types and across a very large dynamic range (dissociation constants from 10−12 M to 10−1 M). We extend an earlier review specifically highlighting advances in sedimentation velocity and sedimentation equilibrium in the analytical Ultracentrifuge applied to protein interactions and mucoadhesion and to review recent applications in protein self-association (tetanus toxoid, agrin), protein-like carbohydrate association (aminocelluloses), carbohydrate-protein interactions (polysaccharide-gliadin), nucleic-acid protein (G-duplexes), nucleic acid-carbohydrate (DNA-chitosan) and finally carbohydrate-carbohydrate (xanthan-chitosan and a ternary polysaccharide complex) interactions.