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Jean-lou Justine - One of the best experts on this subject based on the ideXlab platform.
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Étude Ultrastructurale de la gamétogenèse de Schistosoma bovis Sonsino, 1876 (Trematoda : Schistosomatidae) [Ultrastructural Study of gametogenesis in Schistosoma bovis Sonsino, 1876 (Trematoda: Schistosomatidae)]
2013Co-Authors: Jean-lou JustineAbstract:[Ultrastructural Study of gametogenesis in Schistosoma bovis Sonsino, 1876 (Trematoda: Schistosomatidae)] Text in French with many plates of electron micrographs of spermatogenesis and oogenesis in Schistosoma. PhD Thesis. Thèse de troisième cycle, Université des Sciences et Techniques du Languedoc(Montpellier II), France, 1980. High Resolution Version (exists also in lower resolution http://dx.doi.org/10.6084/m9.figshare.154986)
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Ultrastructural Study of spermiogenesis and the spermatozoon of cavisoma magnum southwell 1927 acanthocephala palaeacanthocephala cavisomidae from siganus lineatus pisces teleostei siganidae valenciennes 1835 in new caledonia
Micron, 2012Co-Authors: Josephine Foata, Yann Quilichini, Jean-lou Justine, Rodney A BrayAbstract:This paper presents an Ultrastructural Study of Cavisoma magnum (Acanthocephala, Cavisomatidae) with a Transmission Electron Microscopy tool. This parasite of the fish Siganus lineatus is here reported for the first time from off New Caledonia, South Pacific. It is the first Study describing the ultrastructure, spermiogenesis and spermatozoon of a species of the family Cavisomatidae. The young spermatid of C. magnum possesses a centriole constituted of doublets without a central element. After the elaboration of a flagellum of 9+2 pattern, the centriole migrates in a nuclear groove. Then the flagellum migration occurs and gives rise to a spermatozoon. The distribution and the size of the protein granules are reported and the posterior extremity appears like a chromatin lamina wave. Comparative Ultrastructural data are presented on sperm and spermiogenesis of the Acanthocephala and Rotifers examined to date and the phylogenetic implications are discussed.
Zhang Cuiping - One of the best experts on this subject based on the ideXlab platform.
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An Ultrastructural Study of Trichophyton rubrum induced onychomycosis
BMC INFECTIOUS DISEASES, 2015Co-Authors: Yue Xueping, Qi Zhang, Li Qing, Wang Hongwei, Sun Yilin, Wang Aiping, Zhang CuipingAbstract:Background: Trichophyton rubrum (T. rubrum) caused onychomycosis is the most common nail fungal disease. The common diagnostic methods are direct microscopic examination and fungal culture. In this Study we used scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to Study the subungual Ultrastructural changes in T. rubrum induced onychomycosis. Methods: Six outpatients with onychomycosis were recruited and T. rubrum infection was confirmed by fungal culture. Six toenail samples were collected and prepared for SEM characterization. The cultured fugal colonies were prepared for SEM and TEM characterization. Results: 1) SEM showed significant structural damages and the formation of a thin layer or a single layer of keratinocytes in all infected nail plates. Hyphae (piercing or penetrating keratinocytes layers), arthrospores and local bacterial aggregation were observed on the ventral surface of the nail plates. 2) SEM of the cultured fungal colony showed relatively straight, highly branched hyphae and microconidias; TEM showed branching hyphae that were composed of double-layer cell walls. Hyphae had nucleus, mitochondria, liposomes, lysosomes, scattered rough endoplasmic reticulum, myeloid bodies and aggregated ribosomes. There were high-density particles outside the hyphae. Conclusion: SEM showed a large number of hyphae penetrated the keratinocytes layer, suggesting that T. rubrum can cause severe damage to the stratum corneum. TEM showed the Ultrastructural features of T. rubrum-induced infection before treatment.SCI(E)PubMedARTICLEbjttpfk6650@hotmail.com15321
Cuiping Zhang - One of the best experts on this subject based on the ideXlab platform.
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an Ultrastructural Study of trichophyton rubrum induced onychomycosis
BMC Infectious Diseases, 2015Co-Authors: Xueping Yue, Hongwei Wang, Yilin Sun, Aiping Wang, Qi Zhang, Cuiping ZhangAbstract:Trichophyton rubrum (T.rubrum) caused onychomycosis is the most common nail fungal disease. The common diagnostic methods are direct microscopic examination and fungal culture. In this Study we used scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to Study the subungual Ultrastructural changes in T. rubrum induced onychomycosis. Six outpatients with onychomycosis were recruited and T.rubrum infection was confirmed by fungal culture. Six toenail samples were collected and prepared for SEM characterization. The cultured fugal colonies were prepared for SEM and TEM characterization. 1) SEM showed significant structural damages and the formation of a thin layer or a single layer of keratinocytes in all infected nail plates. Hyphae (piercing or penetrating keratinocytes layers), arthrospores and local bacterial aggregation were observed on the ventral surface of the nail plates. 2) SEM of the cultured fungal colony showed relatively straight, highly branched hyphae and microconidias; TEM showed branching hyphae that were composed of double-layer cell walls. Hyphae had nucleus, mitochondria, liposomes, lysosomes, scattered rough endoplasmic reticulum, myeloid bodies and aggregated ribosomes. There were high-density particles outside the hyphae. SEM showed a large number of hyphae penetrated the keratinocytes layer, suggesting that T. rubrum can cause severe damage to the stratum corneum. TEM showed the Ultrastructural features of T. rubrum-induced infection before treatment.
Qi Zhang - One of the best experts on this subject based on the ideXlab platform.
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an Ultrastructural Study of trichophyton rubrum induced onychomycosis
BMC Infectious Diseases, 2015Co-Authors: Xueping Yue, Hongwei Wang, Yilin Sun, Aiping Wang, Qi Zhang, Cuiping ZhangAbstract:Trichophyton rubrum (T.rubrum) caused onychomycosis is the most common nail fungal disease. The common diagnostic methods are direct microscopic examination and fungal culture. In this Study we used scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to Study the subungual Ultrastructural changes in T. rubrum induced onychomycosis. Six outpatients with onychomycosis were recruited and T.rubrum infection was confirmed by fungal culture. Six toenail samples were collected and prepared for SEM characterization. The cultured fugal colonies were prepared for SEM and TEM characterization. 1) SEM showed significant structural damages and the formation of a thin layer or a single layer of keratinocytes in all infected nail plates. Hyphae (piercing or penetrating keratinocytes layers), arthrospores and local bacterial aggregation were observed on the ventral surface of the nail plates. 2) SEM of the cultured fungal colony showed relatively straight, highly branched hyphae and microconidias; TEM showed branching hyphae that were composed of double-layer cell walls. Hyphae had nucleus, mitochondria, liposomes, lysosomes, scattered rough endoplasmic reticulum, myeloid bodies and aggregated ribosomes. There were high-density particles outside the hyphae. SEM showed a large number of hyphae penetrated the keratinocytes layer, suggesting that T. rubrum can cause severe damage to the stratum corneum. TEM showed the Ultrastructural features of T. rubrum-induced infection before treatment.
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An Ultrastructural Study of Trichophyton rubrum induced onychomycosis
BMC INFECTIOUS DISEASES, 2015Co-Authors: Yue Xueping, Qi Zhang, Li Qing, Wang Hongwei, Sun Yilin, Wang Aiping, Zhang CuipingAbstract:Background: Trichophyton rubrum (T. rubrum) caused onychomycosis is the most common nail fungal disease. The common diagnostic methods are direct microscopic examination and fungal culture. In this Study we used scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to Study the subungual Ultrastructural changes in T. rubrum induced onychomycosis. Methods: Six outpatients with onychomycosis were recruited and T. rubrum infection was confirmed by fungal culture. Six toenail samples were collected and prepared for SEM characterization. The cultured fugal colonies were prepared for SEM and TEM characterization. Results: 1) SEM showed significant structural damages and the formation of a thin layer or a single layer of keratinocytes in all infected nail plates. Hyphae (piercing or penetrating keratinocytes layers), arthrospores and local bacterial aggregation were observed on the ventral surface of the nail plates. 2) SEM of the cultured fungal colony showed relatively straight, highly branched hyphae and microconidias; TEM showed branching hyphae that were composed of double-layer cell walls. Hyphae had nucleus, mitochondria, liposomes, lysosomes, scattered rough endoplasmic reticulum, myeloid bodies and aggregated ribosomes. There were high-density particles outside the hyphae. Conclusion: SEM showed a large number of hyphae penetrated the keratinocytes layer, suggesting that T. rubrum can cause severe damage to the stratum corneum. TEM showed the Ultrastructural features of T. rubrum-induced infection before treatment.SCI(E)PubMedARTICLEbjttpfk6650@hotmail.com15321
Fabian Mohamed - One of the best experts on this subject based on the ideXlab platform.
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folliculostellate cells in pituitary pars distalis of male viscacha immunohistochemical morphometric and Ultrastructural Study
European Journal of Histochemistry, 2010Co-Authors: Mariano Acosta, Veronica Palmira Filippa, Fabian MohamedAbstract:Folliculostellate cells (FSC) have been reported in pituitary of several mammalian species. FSC morphology and secreted sub- stances have been instrumental to the under- standing of their function. The purpose of this work was to perform an immunohistochemi- cal, morphometric and Ultrastructural Study of the pituitary pars distalis FSC in adult male viscacha and to analyze their relation with hormone secreting cells. Immunohistoche- mistry and image analysis were carried out in different sectors of the gland, from the middle (sector 1) to the glandular periphery (sector 5). Transmission electron microscopy with lanthanum as electrodense tracer was used. FSC formed follicles with PAS-positive colloid inside. They expressed S-100 protein mainly in both nucleus and cytoplasm. FSC were stel- late-like in shape and exhibited short cyto- plasmic processes that contacted with blood vessels and endocrine cells. In addition, some follicular colloids were immunostained with anti-S-100 protein. A few FSC were immunos- tained with anti-glial fibrillary acidic protein (GFAP) and anti-vimentin. The morphometric parameters analyzed (percentages of S-100- positive total, cellular and colloidal areas) increased from sector 1 to sector 3 and then decreased to sector 5. Hormone secreting cells, mainly lactotrophs, gonadotrophs and corticotrophs were associated with FSC and follicles. The Ultrastructural Study demonstrat- ed that FSC developed junctional complexes and desmosomes between their lateral mem- branes. Lanthanum freely penetrated the spaces between granulated cells and FSC, but did not penetrate into the follicular lumen. In conclusion: 1) the differential expression of S- 100 protein, GFAP and vimentin may indicate different physiological stages of FSC; 2) the expression of these proteins suggests a neuroectodermic origin of these cells; 3) FSC spa- tial distribution, association with endocrine cells, and the generation of an intercellular communication network suggest that FSC are involved in the pituitary pars distalis paracrine regulation of the viscacha.
