The Experts below are selected from a list of 183 Experts worldwide ranked by ideXlab platform
Hongxin Gao - One of the best experts on this subject based on the ideXlab platform.
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water induced gel formation of an oleanlic acid adenine conjugate and the effects of Uracil Derivative on the gel stability
Soft Matter, 2012Co-Authors: Chulong Liu, Hongxin GaoAbstract:The conjugate of oleanlic acid with adenine was synthesized and it could be gelled in mixed solvents of THF and water, but no gel was formed in the single organic solvent. The self-aggregation behaviour and physical properties of the organogel were characterized by 1H NMR, FT-IR, and scanning electron microscopy. The morphology and the stability of the gel were remarkably affected by the Uracil Derivative through destroying hydrogen-bonding.
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Water-induced gel formation of an oleanlic acid–adenine conjugate and the effects of Uracil Derivative on the gel stability
Soft Matter, 2012Co-Authors: Chulong Liu, Hongxin GaoAbstract:The conjugate of oleanlic acid with adenine was synthesized and it could be gelled in mixed solvents of THF and water, but no gel was formed in the single organic solvent. The self-aggregation behaviour and physical properties of the organogel were characterized by 1H NMR, FT-IR, and scanning electron microscopy. The morphology and the stability of the gel were remarkably affected by the Uracil Derivative through destroying hydrogen-bonding.
Silvana Raić-malić - One of the best experts on this subject based on the ideXlab platform.
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Discovery of New Acid Ceramidase-Targeted Acyclic 5-Alkynyl and 5-Heteroaryl Uracil Nucleosides
ACS medicinal chemistry letters, 2015Co-Authors: Andrijana Meščić, Anja Harej, Marko Klobučar, Danijel Glavač, Mario Cetina, Sandra Kraljević Pavelić, Silvana Raić-malićAbstract:A series of novel N-acyclic Uracil analogs with linear, branched, aromatic, and cyclopropyl-alkynyl as well as heteroaryl moieties at C-5 were prepared using palladium catalyzed Sonogashira and Stille cross-coupling and evaluated against malignant tumor cell lines. C-5-Furan-2-yl Uracil Derivative 6 was shown to be more potent against MCF-7 than the reference drug 5-fluoroUracil (5-FU), while C-5-alkynyl Uracil Derivatives 9c and 9e exhibited antibreast cancer activities comparable to 5-FU. Selected compounds induced cell death, partially due to apoptosis, of MCF-7 breast cancer cells. Abrogation of acid ceramidase (ASAH1) expression of 9c and 9e indicated that these compounds could perturb ASAH1-mediated sphingolipid signaling. The selective activity of 9c and 9e against breast cancer cells via the ASAH1-mediated signaling, as a molecular target, might have a great advantage for potential future therapeutic use.
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One-pot click synthesis of 1,2,3-triazole-embedded unsaturated Uracil Derivatives and hybrids of 1,5- and 2,5-disubstituted tetrazoles and pyrimidines
Tetrahedron Letters, 2015Co-Authors: Svjetlana Krištafor, Sandra Kraljević Pavelić, Andrea Bistrović, Janez Plavec, Damjan Makuc, Tamara Martinović, Silvana Raić-malićAbstract:Novel conformationally restricted pyrimidine Derivatives with a 1,2,3-triazolyl scaffold bound via Z- and E-2-butenyl spacers were prepared by Cu(I)-catalyzed click chemistry via a one-pot, multi-step reaction under microwave irradiation, while 1,5- and 2,5-disubstituted tetrazoles were synthesized by convenient, environmentally friendly click synthesis and subsequently by N-alkylation of 5-substituted 1H-tetrazoles. Among all the tested compounds, the N-1,N-3-disubstituted olefinic Uracil Derivative showed the highest antiproliferative effects.
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The novel C-5 aryl, alkenyl, and alkynyl substituted Uracil Derivatives of l-ascorbic acid: Synthesis, cytostatic, and antiviral activity evaluations
Bioorganic & medicinal chemistry, 2006Co-Authors: Tatjana Gazivoda, Silvana Raić-malić, Marko Marjanović, Marijeta Kralj, Krešimir Pavelić, Jan Balzarini, Erik De Clercq, Mladen MintasAbstract:The novel C-5 substituted Uracil Derivatives of l-ascorbic acid were synthesized by coupling of 5-iodoUracil-4,5-didehydro-5,6-dideoxy-l-ascorbic acid with unsaturated stannanes under Stille reaction conditions. The new compounds were evaluated for their antitumoral and antiviral activities. Among all compounds evaluated the 5-propynyl substituted Uracil Derivative of l-ascorbic acid (7) exhibited the most pronounced cytostatic activities against all examined tumor cell lines (IC(50): 0.2-0.78 microM). However, this compound was also cytotoxic to human normal fibroblasts WI 38. The 5-(phenylethynyl)Uracil-2,3-di-O-benzylated l-ascorbic acid Derivative (4) exhibited an albeit slight (IC(50): 55-108 microM), but selective inhibitory effect toward all tumor cell lines except for cervical carcinoma (HeLa), pancreatic carcinoma (MiaPaCa-2), laryngeal carcinoma (Hep-2), and colon carcinoma (SW 620), and no cytotoxicity to normal human fibroblast (WI 38). Compound 7 showed some, not highly specific, inhibitory potential against vesicular stomatitis virus, Coxsackie B4 virus, and Sindbis viruses (EC(50): 1.6 microM).
Chulong Liu - One of the best experts on this subject based on the ideXlab platform.
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water induced gel formation of an oleanlic acid adenine conjugate and the effects of Uracil Derivative on the gel stability
Soft Matter, 2012Co-Authors: Chulong Liu, Hongxin GaoAbstract:The conjugate of oleanlic acid with adenine was synthesized and it could be gelled in mixed solvents of THF and water, but no gel was formed in the single organic solvent. The self-aggregation behaviour and physical properties of the organogel were characterized by 1H NMR, FT-IR, and scanning electron microscopy. The morphology and the stability of the gel were remarkably affected by the Uracil Derivative through destroying hydrogen-bonding.
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Water-induced gel formation of an oleanlic acid–adenine conjugate and the effects of Uracil Derivative on the gel stability
Soft Matter, 2012Co-Authors: Chulong Liu, Hongxin GaoAbstract:The conjugate of oleanlic acid with adenine was synthesized and it could be gelled in mixed solvents of THF and water, but no gel was formed in the single organic solvent. The self-aggregation behaviour and physical properties of the organogel were characterized by 1H NMR, FT-IR, and scanning electron microscopy. The morphology and the stability of the gel were remarkably affected by the Uracil Derivative through destroying hydrogen-bonding.
Emmanuel Mikros - One of the best experts on this subject based on the ideXlab platform.
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Tandem virtual screening targeting the SRA domain of UHRF1 identifies a novel chemical tool modulating DNA methylation
European Journal of Medicinal Chemistry, 2016Co-Authors: Vassilios Myrianthopoulos, Pierre-francois Cartron, Zita Liutkeviciute, Saulius Klimasauskas, Daumantas Matulis, Christian Bronner, Nadine Martinet, Emmanuel MikrosAbstract:Ubiquitin-like protein UHRF1 that contains PHD and RING finger domain 1 is a key epigenetic protein enabling maintenance of the DNA methylation status through replication. A tandem virtual screening approach was implemented for identifying small molecules able to bind the 5-methylcytosine pocket of UHRF1 and inhibit its functionality. The NCI/DTP small molecules Repository was screened in silico by a combined protocol implementing structure-based and ligand-based methodologies. Consensus ranking was utilized to select a set of 27 top-ranked compounds that were subsequently evaluated experimentally in a stepwise manner for their ability to demethylate DNA in cellulo using PCR-MS and HPLC-MS/MS. The most active molecules were further assessed in a cell-based setting by the Proximity Ligation In Situ Assay and the ApoTome technology. Both evaluations confirmed that the DNMT1/UHRF1 interactions were significantly reduced after 4 h of incubation of U251 glioma cells with the most potent compound NSC232003, showing a 50% interaction inhibition at 15 mM as well as induction of global DNA cytosine demethylation as measured by ELISA. This is the first report of a chemical tool that targets UHRF1 and modulates DNA methylation in a cell context by potentially disrupting DNMT1/UHRF1 interactions. Compound NSC232003, a Uracil Derivative freely available by the NCI/DTP Repository, provides a versatile lead for developing highly potent and cell-permeable UHRF1 inhibitors that will enable dissection of DNA methylation inheritance.
Carlos E Crespohernandez - One of the best experts on this subject based on the ideXlab platform.
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direct observation of triplet state population dynamics in the rna Uracil Derivative 1 cyclohexylUracil
Journal of Physical Chemistry Letters, 2015Co-Authors: Matthew M Brister, Carlos E CrespohernandezAbstract:Investigation of the excited-state dynamics in nucleic acid monomers is an area of active research due to the crucial role these early events play in DNA and RNA photodamage. The dynamics and rate at which the triplet state is populated are key mechanistic pathways yet to be fully elucidated. Direct spectroscopic evidence is presented in this contribution for intersystem crossing dynamics in a Uracil Derivative, 1-cyclohexylUracil. It is shown that intersystem crossing to the triplet manifold occurs in one picosecond or less in acetonitrile solution—at least an order of magnitude faster than previously estimated experimentally. Broadband transient absorption measurements also reveal the primary electronic relaxation pathways of the Uracil chromophore, including the absorption spectra of the 1ππ*, 1nπ*, and 3ππ* states and the rates of vibrational cooling in the ground and 3ππ* states. The experimental results are supported by density functional calculations.
