The Experts below are selected from a list of 327 Experts worldwide ranked by ideXlab platform
Weichuan Shih - One of the best experts on this subject based on the ideXlab platform.
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reagent and separation free measurements of Urine creatinine concentration using stamping surface enhanced raman scattering s sers
Biomedical Optics Express, 2015Co-Authors: Fusheng Zhao, Jianbo Zeng, Chandra Mohan, Weichuan ShihAbstract:We report a novel reagent- and separation-free method for Urine creatinine concentration measurement using stamping surface enhanced Raman scattering (S-SERS) technique with nanoporous gold disk (NPGD) plasmonic substrates, a label-free, multiplexed molecular sensing and imaging technique recently developed by us. The performance of this new technology is evaluated by the detection and quantification of creatinine spiked in three different liquids: creatinine in water, mixture of creatinine and urea in water, and creatinine in artificial Urine within physiologically relevant concentration ranges. Moreover, the potential application of our method is demonstrated by creatinine concentration measurements in Urine samples collected from a mouse model of nephritis. The limit of detection of creatinine was 13.2 nM (0.15 µg/dl) and 0.68 mg/dl in water and Urine, respectively. Our method would provide an alternative tool for rapid, cost-effective, and reliable Urine Analysis for non-invasive diagnosis and monitoring of renal function.
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reagent and separation free measurements of Urine creatinine concentration using stamping surface enhanced raman scattering s sers
Biomedical Optics Express, 2015Co-Authors: Ming Li, Fusheng Zhao, Jianbo Zeng, Chandra Mohan, Yong Du, Weichuan ShihAbstract:We report a novel reagent- and separation-free method for Urine creatinine concentration measurement using stamping surface enhanced Raman scattering (S-SERS) technique with nanoporous gold disk (NPGD) plasmonic substrates, a label-free, multiplexed molecular sensing and imaging technique recently developed by us. The performance of this new technology is evaluated by the detection and quantification of creatinine spiked in three different liquids: creatinine in water, mixture of creatinine and urea in water, and creatinine in artificial Urine within physiologically relevant concentration ranges. Moreover, the potential application of our method is demonstrated by creatinine concentration measurements in Urine samples collected from a mouse model of nephritis. The limit of detection of creatinine was 13.2 nM (0.15 µg/dl) and 0.68 mg/dl in water and Urine, respectively. Our method would provide an alternative tool for rapid, cost-effective, and reliable Urine Analysis for non-invasive diagnosis and monitoring of renal function.
Mark David Kilby - One of the best experts on this subject based on the ideXlab platform.
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fetal lower urinary tract obstruction
Seminars in Fetal & Neonatal Medicine, 2007Co-Authors: David Lissauer, R K Morris, Mark David KilbyAbstract:Summary Fetal lower urinary tract obstruction affects 2.2 per 10 000 births. It is a consequence of a range of pathological processes, most commonly posterior urethral valves (64%) or urethral atresia (39%). It is a condition of high mortality and morbidity associated with progressive renal dysfunction and oligohydramnios, and hence fetal pulmonary hypoplasia. Accurate detection is possible via ultrasound, but the underlying pathology is often unknown. In future, magnetic resonance imaging (MRI) may be increasingly used alongside ultrasound in the diagnosis and assessment of fetuses with lower urinary tract obstruction. Fetal Urine Analysis may provide improvements in prenatal determination of renal prognosis, but the optimum criteria to be used remain unclear. It is now possible to decompress the obstruction in utero via percutaneous vesico-amniotic shunting or cystoscopic techniques. In appropriately selected fetuses intervention may improve perinatal survival, but long-term renal morbidity amongst survivors remains problematic.
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systematic review of accuracy of fetal Urine Analysis to predict poor postnatal renal function in cases of congenital urinary tract obstruction
Prenatal Diagnosis, 2007Co-Authors: R K Morris, Mark David Kilby, E Quinlanjones, Khalid S KhanAbstract:Objective To evaluate the clinical usefulness of Analysis of fetal Urine in the prediction of poor postnatal renal function in cases of congenital urinary tract obstruction. Methods A systematic review was performed. We conducted extensive electronic searches (database inception–2006). The reference lists of articles obtained were searched for any further articles. Two reviewers independently selected the articles in which the accuracy of fetal urinalysis was evaluated to predict poor postnatal renal function. There were no language restrictions. Data were extracted on study characteristics, quality and results, to construct 2 × 2 tables. Likelihood ratios for positive (LR+) and negative (LR−) test results were generated for the different fetal urinary analytes at various thresholds. Results There were 23 articles that met the selection criteria, including a total of 572 women and 63 2 × 2 tables. The two most accurate tests were calcium > 95th centile for gestation (LR + 6.65, 0.23–190.96; LR − 0.19, 0.05–0.74) and sodium > 95th centile for gestation (LR + 4.46, 1.71–11.6; LR − 0.39, 0.17–0.88). β2-microglobulin was found to be less accurate (LR + 2.92, 1.28–6.69; LR − 0.53, 0.24–1.17). Conclusion The current evidence demonstrates that none of the analytes of fetal Urine investigated so far can be shown to yield clinically significant accuracy to predict poor postnatal renal function. Copyright © 2007 John Wiley & Sons, Ltd.
E Kirowaeisner - One of the best experts on this subject based on the ideXlab platform.
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trace determination of mercury by anodic stripping voltammetry at the rotating gold electrode
Analytica Chimica Acta, 2000Co-Authors: Y Bonfil, M Brand, E KirowaeisnerAbstract:Abstract A simple and highly reliable method for the determination of mercury on a rotating gold disk electrode is reported. The signal is linear with concentration over a wide concentration range (0.2–400 nM). The stability of the electrode is excellent. No mechanical polishing between runs is required and a simple electrochemical pretreatment is applied about once in 100 runs. The detection limit in synthetic solutions, applying the subtractive mode of anodic stripping voltammetry (SASV) is 50 pM for a 120 s deposition time at 5000 rpm and 4 nM in Urine sample for 180 s deposition time. The reproducibility of the analytical signal is better than 2% in solutions containing 1 nM Hg(II). The applicability of the method in Urine Analysis was demonstrated with the use of certified samples. No interference by lead, copper, cadmium, chromium or selenium was found at concentrations corresponding to their toxic occurrence in Urine.
R K Morris - One of the best experts on this subject based on the ideXlab platform.
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fetal lower urinary tract obstruction
Seminars in Fetal & Neonatal Medicine, 2007Co-Authors: David Lissauer, R K Morris, Mark David KilbyAbstract:Summary Fetal lower urinary tract obstruction affects 2.2 per 10 000 births. It is a consequence of a range of pathological processes, most commonly posterior urethral valves (64%) or urethral atresia (39%). It is a condition of high mortality and morbidity associated with progressive renal dysfunction and oligohydramnios, and hence fetal pulmonary hypoplasia. Accurate detection is possible via ultrasound, but the underlying pathology is often unknown. In future, magnetic resonance imaging (MRI) may be increasingly used alongside ultrasound in the diagnosis and assessment of fetuses with lower urinary tract obstruction. Fetal Urine Analysis may provide improvements in prenatal determination of renal prognosis, but the optimum criteria to be used remain unclear. It is now possible to decompress the obstruction in utero via percutaneous vesico-amniotic shunting or cystoscopic techniques. In appropriately selected fetuses intervention may improve perinatal survival, but long-term renal morbidity amongst survivors remains problematic.
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systematic review of accuracy of fetal Urine Analysis to predict poor postnatal renal function in cases of congenital urinary tract obstruction
Prenatal Diagnosis, 2007Co-Authors: R K Morris, Mark David Kilby, E Quinlanjones, Khalid S KhanAbstract:Objective To evaluate the clinical usefulness of Analysis of fetal Urine in the prediction of poor postnatal renal function in cases of congenital urinary tract obstruction. Methods A systematic review was performed. We conducted extensive electronic searches (database inception–2006). The reference lists of articles obtained were searched for any further articles. Two reviewers independently selected the articles in which the accuracy of fetal urinalysis was evaluated to predict poor postnatal renal function. There were no language restrictions. Data were extracted on study characteristics, quality and results, to construct 2 × 2 tables. Likelihood ratios for positive (LR+) and negative (LR−) test results were generated for the different fetal urinary analytes at various thresholds. Results There were 23 articles that met the selection criteria, including a total of 572 women and 63 2 × 2 tables. The two most accurate tests were calcium > 95th centile for gestation (LR + 6.65, 0.23–190.96; LR − 0.19, 0.05–0.74) and sodium > 95th centile for gestation (LR + 4.46, 1.71–11.6; LR − 0.39, 0.17–0.88). β2-microglobulin was found to be less accurate (LR + 2.92, 1.28–6.69; LR − 0.53, 0.24–1.17). Conclusion The current evidence demonstrates that none of the analytes of fetal Urine investigated so far can be shown to yield clinically significant accuracy to predict poor postnatal renal function. Copyright © 2007 John Wiley & Sons, Ltd.
Fusheng Zhao - One of the best experts on this subject based on the ideXlab platform.
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reagent and separation free measurements of Urine creatinine concentration using stamping surface enhanced raman scattering s sers
Biomedical Optics Express, 2015Co-Authors: Fusheng Zhao, Jianbo Zeng, Chandra Mohan, Weichuan ShihAbstract:We report a novel reagent- and separation-free method for Urine creatinine concentration measurement using stamping surface enhanced Raman scattering (S-SERS) technique with nanoporous gold disk (NPGD) plasmonic substrates, a label-free, multiplexed molecular sensing and imaging technique recently developed by us. The performance of this new technology is evaluated by the detection and quantification of creatinine spiked in three different liquids: creatinine in water, mixture of creatinine and urea in water, and creatinine in artificial Urine within physiologically relevant concentration ranges. Moreover, the potential application of our method is demonstrated by creatinine concentration measurements in Urine samples collected from a mouse model of nephritis. The limit of detection of creatinine was 13.2 nM (0.15 µg/dl) and 0.68 mg/dl in water and Urine, respectively. Our method would provide an alternative tool for rapid, cost-effective, and reliable Urine Analysis for non-invasive diagnosis and monitoring of renal function.
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reagent and separation free measurements of Urine creatinine concentration using stamping surface enhanced raman scattering s sers
Biomedical Optics Express, 2015Co-Authors: Ming Li, Fusheng Zhao, Jianbo Zeng, Chandra Mohan, Yong Du, Weichuan ShihAbstract:We report a novel reagent- and separation-free method for Urine creatinine concentration measurement using stamping surface enhanced Raman scattering (S-SERS) technique with nanoporous gold disk (NPGD) plasmonic substrates, a label-free, multiplexed molecular sensing and imaging technique recently developed by us. The performance of this new technology is evaluated by the detection and quantification of creatinine spiked in three different liquids: creatinine in water, mixture of creatinine and urea in water, and creatinine in artificial Urine within physiologically relevant concentration ranges. Moreover, the potential application of our method is demonstrated by creatinine concentration measurements in Urine samples collected from a mouse model of nephritis. The limit of detection of creatinine was 13.2 nM (0.15 µg/dl) and 0.68 mg/dl in water and Urine, respectively. Our method would provide an alternative tool for rapid, cost-effective, and reliable Urine Analysis for non-invasive diagnosis and monitoring of renal function.
