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Michał J. Markuszewski - One of the best experts on this subject based on the ideXlab platform.

  • Metabolomic Heterogeneity of Urogenital Tract Cancers Analyzed by Complementary Chromatographic Techniques Coupled with Mass Spectrometry.
    Current medicinal chemistry, 2019
    Co-Authors: Arlette Yumba-mpanga, Roman Kaliszan, Wiktoria Struck-lewicka, Marcin Markuszewski, Renata Wawrzyniak, Marek Roslan, Michał J. Markuszewski
    Abstract:

    BACKGROUND In regard to Urogenital Tract Cancer studies, an estimated 340,650 new cases and 58,360 deaths from genital system Cancer and about 141,140 new cases and 29330 deaths from urinary system were projected to occur in the United States in 2012. The main drawbacks of currently available diagnostic tests constitute the low specificity, costliness and quite high invasiveness. OBJECTIVE The main goal of this pilot study was to determine and compare urine metabolic fingerprints in Urogenital Tract Cancer patients and healthy controls. METHOD A comparative analysis of the metabolic profile of urine from 30 patients with Cancer of the genitourinary system (bladder (n=10), kidney (n=10) and prostate (n=10)) and 30 healthy volunteers as a control group was provided by LC-TOF/MS and GCQqQ/ MS. The data analysis was performed by the use of U-Mann Whitney test or Student's t-test, principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA). RESULTS As a result, 33, 43, and 22 compounds were identified as statistically significant in bladder, prostate and kidney Cancer, respectively, compared to healthy groups. CONCLUSION Diverse compounds such as purine, sugars, amino acids, nucleosides, organic acids which play a role in purine metabolism, in tricarboxylic acid cycle, in amino acid metabolism or in gut microbiota metabolism were identified. Only two metabolites namely glucocaffeic acid and lactic acid were found to be in common in studied three types of Cancer.

  • Statistical-based approach in potential diagnostic application of urinary nucleosides in Urogenital Tract Cancer
    Biomarkers in medicine, 2015
    Co-Authors: Emilia Daghir-wojtkowiak, Roman Kaliszan, Wiktoria Struck-lewicka, Małgorzata Waszczuk-jankowska, Marcin Markuszewski, Michał J. Markuszewski
    Abstract:

    We aimed at evaluation the potential diagnostic role of urinary nucleosides in Urogenital Tract Cancer. Concentrations of 12 nucleosides determined by LC-MS/MS were subjected to correlation, association and interaction analyses. We identified six pairs of nucleosides differently correlated in the group of patients and controls (p < 0.05). N-2-methylguanosine (odds ratio: 4.82; 95% CI: 1.78-12.93; p = 0.002) and N,N-dimethylguanosine (odds ratio: 5.45; 95% CI: 1.78-16.44; p = 0.003), were significantly associated with the disease risk (p-corrected = 0.004). Interaction between N-2-methylguanosine and adenosine (p-interaction = 0.019) suggested their multiplicative effect on the outcome. Urinary nucleosides, namely N,N-dimethylguanosine and N-2-methylguanosine may have the potential to serve as prognostic biomarkers. Gender-specific differences in Urogenital Tract Cancer are likely to occur.

  • advanced assessment of the endogenous hormone level as a potential biomarker of the Urogenital Tract Cancer
    Combinatorial Chemistry & High Throughput Screening, 2013
    Co-Authors: Lucyna Konieczna, Mariusz Belka, Tomasz Baczek, Mateusz Ruszkowski, Wiktoria Struck, Michał J. Markuszewski, Roman Kaliszan
    Abstract:

    The evaluation of the relationships between the hormones involved in the Urogenital Tract Cancer, including bladder, kidney, prostate, and testis, could prove important from diagnostic point of view. The determination of the steroid hormone profiles may likely provide a biomarker for discrimination of hormone-related diseases, as well as for differentiation of healthy volunteers from patients with Cancer. The aim of the study was to demonstrate the changes in the steroid hormone profile (comprising corticosteroids, androgens and progesterone) in the urine of patients with the Urogenital Tract Cancer versus urine from healthy subjects. A reliable analytical method based on liquid chromatography coupled with mass spectrometry was successfully applied to determine the urinary profiles of 6 endogenous steroids: cortisol, cortisone, corticosterone, testosterone, epitestosterone and progesterone for 92 Urogenital Tract Cancer patients and 100 healthy controls. The obtained data was further evaluated by in-depth chemometric analysis, including the applied standardized Kennard-Stone's algorithm to pre-process the data. Mann-Whitney U test revealed statistically significant (p <0.05) differences in concentration of androgens and progesterone in the case of bladder Cancer for male and female population, for male also cortisol and cortisone levels were significantly increased. PCA analysis proved a reasonable trend for differentiating healthy and Cancer patients, and finally, applying PLS-DA model we were able to correctly classify 80.56%of Cancer patients. Our results indicate that steroid hormone profile determination could be a promising approach for early diagnosis of Urogenital Tract Cancer. However our preliminary results require an extension both in patient number and steroid profile.

  • Advanced assessment of the endogenous hormone level as a potential biomarker of the Urogenital Tract Cancer.
    Combinatorial chemistry & high throughput screening, 2013
    Co-Authors: Lucyna Konieczna, Mariusz Belka, Tomasz Baczek, Mateusz Ruszkowski, Wiktoria Struck, Michał J. Markuszewski, Roman Kaliszan
    Abstract:

    The evaluation of the relationships between the hormones involved in the Urogenital Tract Cancer, including bladder, kidney, prostate, and testis, could prove important from diagnostic point of view. The determination of the steroid hormone profiles may likely provide a biomarker for discrimination of hormone-related diseases, as well as for differentiation of healthy volunteers from patients with Cancer. The aim of the study was to demonstrate the changes in the steroid hormone profile (comprising corticosteroids, androgens and progesterone) in the urine of patients with the Urogenital Tract Cancer versus urine from healthy subjects. A reliable analytical method based on liquid chromatography coupled with mass spectrometry was successfully applied to determine the urinary profiles of 6 endogenous steroids: cortisol, cortisone, corticosterone, testosterone, epitestosterone and progesterone for 92 Urogenital Tract Cancer patients and 100 healthy controls. The obtained data was further evaluated by in-depth chemometric analysis, including the applied standardized Kennard-Stone's algorithm to pre-process the data. Mann-Whitney U test revealed statistically significant (p

  • Steroid profiles as potential biomarkers in patients with Urogenital Tract Cancer for diagnostic investigations analyzed by liquid chromatography coupled to mass spectrometry
    Journal of pharmaceutical and biomedical analysis, 2012
    Co-Authors: Lucyna Konieczna, Mariusz Belka, Wiktoria Struck, Michał J. Markuszewski, Marcin Markuszewski, Tomasz Bączek, Anna Fel, Roman Kaliszan
    Abstract:

    Large discrepancy remains for the hormone-responsible Cancers with regards to the conditions generating the optimal opportunity for Cancerogenesis. In the research, altered steroid profiles were observed in patients with Urogenital Tract Cancer diseases, namely bladder, kidney, prostate and testis ones. The presented steroid profiles from 154 subjects, including 77 Urogenital Tract Cancer patient and 77 healthy controls were determined by liquid chromatography coupled to mass spectrometry method. Because the original experimental data obtained as a result of analytical experiment in order to interpret them in better way required the appropriate pre-treatment, the data were standardized by scaling and centering. In order to determine which samples form a collection for a high-capacity predictive model, Kennard-Stone's algorithm was used. A principal component analysis of preprocessed data provided better consistency of the steroid profiles with health status of subjects than PCA profiles without data preprocessing and showed a tendency to separate clusters of Cancer patients from healthy subjects. The discriminant analysis was also performed and the percent of correct classification of Cancer patients and control group was calculated. Finally, detailed studies examined the role of steroid profiles measured in urine, and considered as potential biomarkers related to Urogenital Cancer and associated renal dysfunctions.

Roman Kaliszan - One of the best experts on this subject based on the ideXlab platform.

  • Metabolomic Heterogeneity of Urogenital Tract Cancers Analyzed by Complementary Chromatographic Techniques Coupled with Mass Spectrometry.
    Current medicinal chemistry, 2019
    Co-Authors: Arlette Yumba-mpanga, Roman Kaliszan, Wiktoria Struck-lewicka, Marcin Markuszewski, Renata Wawrzyniak, Marek Roslan, Michał J. Markuszewski
    Abstract:

    BACKGROUND In regard to Urogenital Tract Cancer studies, an estimated 340,650 new cases and 58,360 deaths from genital system Cancer and about 141,140 new cases and 29330 deaths from urinary system were projected to occur in the United States in 2012. The main drawbacks of currently available diagnostic tests constitute the low specificity, costliness and quite high invasiveness. OBJECTIVE The main goal of this pilot study was to determine and compare urine metabolic fingerprints in Urogenital Tract Cancer patients and healthy controls. METHOD A comparative analysis of the metabolic profile of urine from 30 patients with Cancer of the genitourinary system (bladder (n=10), kidney (n=10) and prostate (n=10)) and 30 healthy volunteers as a control group was provided by LC-TOF/MS and GCQqQ/ MS. The data analysis was performed by the use of U-Mann Whitney test or Student's t-test, principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA). RESULTS As a result, 33, 43, and 22 compounds were identified as statistically significant in bladder, prostate and kidney Cancer, respectively, compared to healthy groups. CONCLUSION Diverse compounds such as purine, sugars, amino acids, nucleosides, organic acids which play a role in purine metabolism, in tricarboxylic acid cycle, in amino acid metabolism or in gut microbiota metabolism were identified. Only two metabolites namely glucocaffeic acid and lactic acid were found to be in common in studied three types of Cancer.

  • statistical based approach in potential diagnostic application of urinary nucleosides in Urogenital Tract Cancer
    Biomarkers in Medicine, 2015
    Co-Authors: Emilia Daghirwojtkowiak, Roman Kaliszan, Marcin Markuszewski, Wiktoria Strucklewicka, Malgorzata Waszczukjankowska
    Abstract:

    Aim: We aimed at evaluation the potential diagnostic role of urinary nucleosides in Urogenital Tract Cancer. Materials & methods: Concentrations of 12 nucleosides determined by LC-MS/MS were subjected to correlation, association and interaction analyses. Results: We identified six pairs of nucleosides differently correlated in the group of patients and controls (p < 0.05). N-2-methylguanosine (odds ratio: 4.82; 95% CI: 1.78–12.93; p = 0.002) and N,N-dimethylguanosine (odds ratio: 5.45; 95% CI: 1.78–16.44; p = 0.003), were significantly associated with the disease risk (p-corrected = 0.004). Interaction between N-2-methylguanosine and adenosine (p-interaction = 0.019) suggested their multiplicative effect on the outcome. Conclusion: Urinary nucleosides, namely N,N-dimethylguanosine and N-2-methylguanosine may have the potential to serve as prognostic biomarkers. Gender-specific differences in Urogenital Tract Cancer are likely to occur.

  • Statistical-based approach in potential diagnostic application of urinary nucleosides in Urogenital Tract Cancer
    Biomarkers in medicine, 2015
    Co-Authors: Emilia Daghir-wojtkowiak, Roman Kaliszan, Wiktoria Struck-lewicka, Małgorzata Waszczuk-jankowska, Marcin Markuszewski, Michał J. Markuszewski
    Abstract:

    We aimed at evaluation the potential diagnostic role of urinary nucleosides in Urogenital Tract Cancer. Concentrations of 12 nucleosides determined by LC-MS/MS were subjected to correlation, association and interaction analyses. We identified six pairs of nucleosides differently correlated in the group of patients and controls (p < 0.05). N-2-methylguanosine (odds ratio: 4.82; 95% CI: 1.78-12.93; p = 0.002) and N,N-dimethylguanosine (odds ratio: 5.45; 95% CI: 1.78-16.44; p = 0.003), were significantly associated with the disease risk (p-corrected = 0.004). Interaction between N-2-methylguanosine and adenosine (p-interaction = 0.019) suggested their multiplicative effect on the outcome. Urinary nucleosides, namely N,N-dimethylguanosine and N-2-methylguanosine may have the potential to serve as prognostic biomarkers. Gender-specific differences in Urogenital Tract Cancer are likely to occur.

  • advanced assessment of the endogenous hormone level as a potential biomarker of the Urogenital Tract Cancer
    Combinatorial Chemistry & High Throughput Screening, 2013
    Co-Authors: Lucyna Konieczna, Mariusz Belka, Tomasz Baczek, Mateusz Ruszkowski, Wiktoria Struck, Michał J. Markuszewski, Roman Kaliszan
    Abstract:

    The evaluation of the relationships between the hormones involved in the Urogenital Tract Cancer, including bladder, kidney, prostate, and testis, could prove important from diagnostic point of view. The determination of the steroid hormone profiles may likely provide a biomarker for discrimination of hormone-related diseases, as well as for differentiation of healthy volunteers from patients with Cancer. The aim of the study was to demonstrate the changes in the steroid hormone profile (comprising corticosteroids, androgens and progesterone) in the urine of patients with the Urogenital Tract Cancer versus urine from healthy subjects. A reliable analytical method based on liquid chromatography coupled with mass spectrometry was successfully applied to determine the urinary profiles of 6 endogenous steroids: cortisol, cortisone, corticosterone, testosterone, epitestosterone and progesterone for 92 Urogenital Tract Cancer patients and 100 healthy controls. The obtained data was further evaluated by in-depth chemometric analysis, including the applied standardized Kennard-Stone's algorithm to pre-process the data. Mann-Whitney U test revealed statistically significant (p <0.05) differences in concentration of androgens and progesterone in the case of bladder Cancer for male and female population, for male also cortisol and cortisone levels were significantly increased. PCA analysis proved a reasonable trend for differentiating healthy and Cancer patients, and finally, applying PLS-DA model we were able to correctly classify 80.56%of Cancer patients. Our results indicate that steroid hormone profile determination could be a promising approach for early diagnosis of Urogenital Tract Cancer. However our preliminary results require an extension both in patient number and steroid profile.

  • Advanced assessment of the endogenous hormone level as a potential biomarker of the Urogenital Tract Cancer.
    Combinatorial chemistry & high throughput screening, 2013
    Co-Authors: Lucyna Konieczna, Mariusz Belka, Tomasz Baczek, Mateusz Ruszkowski, Wiktoria Struck, Michał J. Markuszewski, Roman Kaliszan
    Abstract:

    The evaluation of the relationships between the hormones involved in the Urogenital Tract Cancer, including bladder, kidney, prostate, and testis, could prove important from diagnostic point of view. The determination of the steroid hormone profiles may likely provide a biomarker for discrimination of hormone-related diseases, as well as for differentiation of healthy volunteers from patients with Cancer. The aim of the study was to demonstrate the changes in the steroid hormone profile (comprising corticosteroids, androgens and progesterone) in the urine of patients with the Urogenital Tract Cancer versus urine from healthy subjects. A reliable analytical method based on liquid chromatography coupled with mass spectrometry was successfully applied to determine the urinary profiles of 6 endogenous steroids: cortisol, cortisone, corticosterone, testosterone, epitestosterone and progesterone for 92 Urogenital Tract Cancer patients and 100 healthy controls. The obtained data was further evaluated by in-depth chemometric analysis, including the applied standardized Kennard-Stone's algorithm to pre-process the data. Mann-Whitney U test revealed statistically significant (p

Marcin Markuszewski - One of the best experts on this subject based on the ideXlab platform.

  • statistical based approach in potential diagnostic application of urinary nucleosides in Urogenital Tract Cancer
    Biomarkers in Medicine, 2015
    Co-Authors: Emilia Daghirwojtkowiak, Roman Kaliszan, Marcin Markuszewski, Wiktoria Strucklewicka, Malgorzata Waszczukjankowska
    Abstract:

    Aim: We aimed at evaluation the potential diagnostic role of urinary nucleosides in Urogenital Tract Cancer. Materials & methods: Concentrations of 12 nucleosides determined by LC-MS/MS were subjected to correlation, association and interaction analyses. Results: We identified six pairs of nucleosides differently correlated in the group of patients and controls (p < 0.05). N-2-methylguanosine (odds ratio: 4.82; 95% CI: 1.78–12.93; p = 0.002) and N,N-dimethylguanosine (odds ratio: 5.45; 95% CI: 1.78–16.44; p = 0.003), were significantly associated with the disease risk (p-corrected = 0.004). Interaction between N-2-methylguanosine and adenosine (p-interaction = 0.019) suggested their multiplicative effect on the outcome. Conclusion: Urinary nucleosides, namely N,N-dimethylguanosine and N-2-methylguanosine may have the potential to serve as prognostic biomarkers. Gender-specific differences in Urogenital Tract Cancer are likely to occur.

  • Statistical-based approach in potential diagnostic application of urinary nucleosides in Urogenital Tract Cancer
    Biomarkers in medicine, 2015
    Co-Authors: Emilia Daghir-wojtkowiak, Roman Kaliszan, Wiktoria Struck-lewicka, Małgorzata Waszczuk-jankowska, Marcin Markuszewski, Michał J. Markuszewski
    Abstract:

    We aimed at evaluation the potential diagnostic role of urinary nucleosides in Urogenital Tract Cancer. Concentrations of 12 nucleosides determined by LC-MS/MS were subjected to correlation, association and interaction analyses. We identified six pairs of nucleosides differently correlated in the group of patients and controls (p < 0.05). N-2-methylguanosine (odds ratio: 4.82; 95% CI: 1.78-12.93; p = 0.002) and N,N-dimethylguanosine (odds ratio: 5.45; 95% CI: 1.78-16.44; p = 0.003), were significantly associated with the disease risk (p-corrected = 0.004). Interaction between N-2-methylguanosine and adenosine (p-interaction = 0.019) suggested their multiplicative effect on the outcome. Urinary nucleosides, namely N,N-dimethylguanosine and N-2-methylguanosine may have the potential to serve as prognostic biomarkers. Gender-specific differences in Urogenital Tract Cancer are likely to occur.

Lucyna Konieczna - One of the best experts on this subject based on the ideXlab platform.

  • advanced assessment of the endogenous hormone level as a potential biomarker of the Urogenital Tract Cancer
    Combinatorial Chemistry & High Throughput Screening, 2013
    Co-Authors: Lucyna Konieczna, Mariusz Belka, Tomasz Baczek, Mateusz Ruszkowski, Wiktoria Struck, Michał J. Markuszewski, Roman Kaliszan
    Abstract:

    The evaluation of the relationships between the hormones involved in the Urogenital Tract Cancer, including bladder, kidney, prostate, and testis, could prove important from diagnostic point of view. The determination of the steroid hormone profiles may likely provide a biomarker for discrimination of hormone-related diseases, as well as for differentiation of healthy volunteers from patients with Cancer. The aim of the study was to demonstrate the changes in the steroid hormone profile (comprising corticosteroids, androgens and progesterone) in the urine of patients with the Urogenital Tract Cancer versus urine from healthy subjects. A reliable analytical method based on liquid chromatography coupled with mass spectrometry was successfully applied to determine the urinary profiles of 6 endogenous steroids: cortisol, cortisone, corticosterone, testosterone, epitestosterone and progesterone for 92 Urogenital Tract Cancer patients and 100 healthy controls. The obtained data was further evaluated by in-depth chemometric analysis, including the applied standardized Kennard-Stone's algorithm to pre-process the data. Mann-Whitney U test revealed statistically significant (p <0.05) differences in concentration of androgens and progesterone in the case of bladder Cancer for male and female population, for male also cortisol and cortisone levels were significantly increased. PCA analysis proved a reasonable trend for differentiating healthy and Cancer patients, and finally, applying PLS-DA model we were able to correctly classify 80.56%of Cancer patients. Our results indicate that steroid hormone profile determination could be a promising approach for early diagnosis of Urogenital Tract Cancer. However our preliminary results require an extension both in patient number and steroid profile.

  • Advanced assessment of the endogenous hormone level as a potential biomarker of the Urogenital Tract Cancer.
    Combinatorial chemistry & high throughput screening, 2013
    Co-Authors: Lucyna Konieczna, Mariusz Belka, Tomasz Baczek, Mateusz Ruszkowski, Wiktoria Struck, Michał J. Markuszewski, Roman Kaliszan
    Abstract:

    The evaluation of the relationships between the hormones involved in the Urogenital Tract Cancer, including bladder, kidney, prostate, and testis, could prove important from diagnostic point of view. The determination of the steroid hormone profiles may likely provide a biomarker for discrimination of hormone-related diseases, as well as for differentiation of healthy volunteers from patients with Cancer. The aim of the study was to demonstrate the changes in the steroid hormone profile (comprising corticosteroids, androgens and progesterone) in the urine of patients with the Urogenital Tract Cancer versus urine from healthy subjects. A reliable analytical method based on liquid chromatography coupled with mass spectrometry was successfully applied to determine the urinary profiles of 6 endogenous steroids: cortisol, cortisone, corticosterone, testosterone, epitestosterone and progesterone for 92 Urogenital Tract Cancer patients and 100 healthy controls. The obtained data was further evaluated by in-depth chemometric analysis, including the applied standardized Kennard-Stone's algorithm to pre-process the data. Mann-Whitney U test revealed statistically significant (p

  • Steroid profiles as potential biomarkers in patients with Urogenital Tract Cancer for diagnostic investigations analyzed by liquid chromatography coupled to mass spectrometry
    Journal of pharmaceutical and biomedical analysis, 2012
    Co-Authors: Lucyna Konieczna, Mariusz Belka, Wiktoria Struck, Michał J. Markuszewski, Marcin Markuszewski, Tomasz Bączek, Anna Fel, Roman Kaliszan
    Abstract:

    Large discrepancy remains for the hormone-responsible Cancers with regards to the conditions generating the optimal opportunity for Cancerogenesis. In the research, altered steroid profiles were observed in patients with Urogenital Tract Cancer diseases, namely bladder, kidney, prostate and testis ones. The presented steroid profiles from 154 subjects, including 77 Urogenital Tract Cancer patient and 77 healthy controls were determined by liquid chromatography coupled to mass spectrometry method. Because the original experimental data obtained as a result of analytical experiment in order to interpret them in better way required the appropriate pre-treatment, the data were standardized by scaling and centering. In order to determine which samples form a collection for a high-capacity predictive model, Kennard-Stone's algorithm was used. A principal component analysis of preprocessed data provided better consistency of the steroid profiles with health status of subjects than PCA profiles without data preprocessing and showed a tendency to separate clusters of Cancer patients from healthy subjects. The discriminant analysis was also performed and the percent of correct classification of Cancer patients and control group was calculated. Finally, detailed studies examined the role of steroid profiles measured in urine, and considered as potential biomarkers related to Urogenital Cancer and associated renal dysfunctions.

Marcin Markuszewski - One of the best experts on this subject based on the ideXlab platform.

  • Metabolomic Heterogeneity of Urogenital Tract Cancers Analyzed by Complementary Chromatographic Techniques Coupled with Mass Spectrometry.
    Current medicinal chemistry, 2019
    Co-Authors: Arlette Yumba-mpanga, Roman Kaliszan, Wiktoria Struck-lewicka, Marcin Markuszewski, Renata Wawrzyniak, Marek Roslan, Michał J. Markuszewski
    Abstract:

    BACKGROUND In regard to Urogenital Tract Cancer studies, an estimated 340,650 new cases and 58,360 deaths from genital system Cancer and about 141,140 new cases and 29330 deaths from urinary system were projected to occur in the United States in 2012. The main drawbacks of currently available diagnostic tests constitute the low specificity, costliness and quite high invasiveness. OBJECTIVE The main goal of this pilot study was to determine and compare urine metabolic fingerprints in Urogenital Tract Cancer patients and healthy controls. METHOD A comparative analysis of the metabolic profile of urine from 30 patients with Cancer of the genitourinary system (bladder (n=10), kidney (n=10) and prostate (n=10)) and 30 healthy volunteers as a control group was provided by LC-TOF/MS and GCQqQ/ MS. The data analysis was performed by the use of U-Mann Whitney test or Student's t-test, principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA). RESULTS As a result, 33, 43, and 22 compounds were identified as statistically significant in bladder, prostate and kidney Cancer, respectively, compared to healthy groups. CONCLUSION Diverse compounds such as purine, sugars, amino acids, nucleosides, organic acids which play a role in purine metabolism, in tricarboxylic acid cycle, in amino acid metabolism or in gut microbiota metabolism were identified. Only two metabolites namely glucocaffeic acid and lactic acid were found to be in common in studied three types of Cancer.

  • Steroid profiles as potential biomarkers in patients with Urogenital Tract Cancer for diagnostic investigations analyzed by liquid chromatography coupled to mass spectrometry
    Journal of pharmaceutical and biomedical analysis, 2012
    Co-Authors: Lucyna Konieczna, Mariusz Belka, Wiktoria Struck, Michał J. Markuszewski, Marcin Markuszewski, Tomasz Bączek, Anna Fel, Roman Kaliszan
    Abstract:

    Large discrepancy remains for the hormone-responsible Cancers with regards to the conditions generating the optimal opportunity for Cancerogenesis. In the research, altered steroid profiles were observed in patients with Urogenital Tract Cancer diseases, namely bladder, kidney, prostate and testis ones. The presented steroid profiles from 154 subjects, including 77 Urogenital Tract Cancer patient and 77 healthy controls were determined by liquid chromatography coupled to mass spectrometry method. Because the original experimental data obtained as a result of analytical experiment in order to interpret them in better way required the appropriate pre-treatment, the data were standardized by scaling and centering. In order to determine which samples form a collection for a high-capacity predictive model, Kennard-Stone's algorithm was used. A principal component analysis of preprocessed data provided better consistency of the steroid profiles with health status of subjects than PCA profiles without data preprocessing and showed a tendency to separate clusters of Cancer patients from healthy subjects. The discriminant analysis was also performed and the percent of correct classification of Cancer patients and control group was calculated. Finally, detailed studies examined the role of steroid profiles measured in urine, and considered as potential biomarkers related to Urogenital Cancer and associated renal dysfunctions.

  • Altered levels of nucleoside metabolite profiles in Urogenital Tract Cancer measured by capillary electrophoresis
    Journal of pharmaceutical and biomedical analysis, 2010
    Co-Authors: Ewa Szymańska, Michał J. Markuszewski, Marcin Markuszewski, Roman Kaliszan
    Abstract:

    Metabolic profiles of nucleosides studied on the level of urine are closely related to the pathophysiological status of the organism. Posttranscriptional modifications of RNA (mostly tRNA) in cell nucleus are responsible for change of nucleoside levels during malignant disease. In this paper, 256 metabolite profiles from 160 Urogenital Tract Cancer patients and 96 healthy controls, composed of 19 nucleosides were collected and studied with the application of such an approach. This approach comprised of the analysis of urine exTracts and the investigation of collected nucleoside and modified nucleoside profiles by advanced statistical data processing tools such as principal component analysis (PCA), hierarchical cluster analysis (HCA), K-Nearest Neighbor method (kNN) and partial least squares-discriminant analysis with probabilistic function (p-PLS-DA). It has been shown that alterations of metabolite profiles in Cancer diseases are mainly expressed by the fold change of the urine levels of most nucleosides. In addition, observed metabolite-to-metabolite ratios differ in Urogenital Cancer patients compared to healthy controls. The obtained relationships between urinary nucleoside profiles and the presence of Cancer diseases have been evaluated. Discrimination of the Cancer patients and the non-Cancer healthy subjects is with 76.5% sensitivity and 80.2% specificity. The presented results prove the usefulness of the metabolomic approach in studying urinary nucleoside profiles with high diagnostic potency in Urogenital Cancer diseases. Profiles of urinary nucleosides might be employed as a reliable and convenient tool in the diagnostics of Urogenital Tract Cancer diseases.