The Experts below are selected from a list of 1056 Experts worldwide ranked by ideXlab platform
Mongan, Nigel P. - One of the best experts on this subject based on the ideXlab platform.
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Histological and immunohistochemical investigation of canine prostate carcinoma with identification of common intraductal carcinoma component
'Wiley', 2021Co-Authors: Brot Simone, Lothion‐roy Jennifer, Grau‐roma Llorenç, White Emily, Guscetti Franco, Rubin, Mark A., Mongan, Nigel P.Abstract:A limited number of species, including men and dogs, spontaneously develop prostate cancer (PC). The histological and molecular relevance of canine PC as a model for the disease in men remains controversial. To address this challenge, this study aimed to assess the histomorphology and expression of basal cell, urothelial and neuroendocrine markers [p63, high molecular weight cytokeratin (HMWCK), Uroplakin 3 (UPIII), neuron-specific enolase (NSE)] in canine PC (n = 41). Based on histomorphology, 10/41 (24%), 21/41 (51%) and 9/41 (22%) were classified as adenocarcinoma (AC), urothelial carcinoma (UC), and mixed carcinoma, respectively. Tumour inflammation was common, frequently severe [20/41 (49%)], and associated with neutering (p
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Histological and immunohistochemical investigation of canine prostate carcinoma with identification of common intraductal carcinoma component
'Wiley', 2021Co-Authors: De Brot Simone, Lothion‐roy Jennifer, Grau‐roma Llorenç, White Emily, Guscetti Franco, Rubin, Mark A., Mongan, Nigel P.Abstract:A limited number of species, including men and dogs, spontaneously develop prostate cancer (PC). The histological and molecular relevance of canine PC as a model for the disease in men remains controversial. To address this challenge, this study aimed to assess the histomorphology and expression of basal cell, urothelial and neuroendocrine markers [p63, high molecular weight cytokeratin (HMWCK), Uroplakin 3 (UPIII), neuron-specific enolase (NSE)] in canine PC (n = 41). Based on histomorphology, 10/41 (24%), 21/41 (51%) and 9/41 (22%) were classified as adenocarcinoma (AC), urothelial carcinoma (UC), and mixed carcinoma, respectively. Tumour inflammation was common, frequently severe [20/41 (49%)], and associated with neutering (p < .02) and urothelial differentiation (p < .02). Most (36/40, 90%) cancers contained only rare cells with basal cell marker expression or were negative. The expression of UPIII was absent or weak in the majority (33/38, 87%) of tumours, with moderate to strong staining in the remaining cases. NSE expression in PC was rare and limited to 2/14 (14%) cases. Tumour extension into benign ducts and glands was a common finding with presence in 17/39 (44%) of carcinomas with and without urothelial differentiation. In conclusion, we confirm that canine PC is characterized by absent or weak expression of basal cell and urothelial markers. Although rare, NSE expression, potentially indicating neuroendocrine differentiation, is reported for the first time in canine PCa. Intraductal carcinoma of the prostate with concurrent invasive PCa (IDCP-inv) is a frequent, not previously described, finding in dogs with PC
Peter Frey - One of the best experts on this subject based on the ideXlab platform.
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Porcine clonal urothelial cells arising from a single urethral cell.
2014Co-Authors: Hans M. Larsson, Francois Gorostidi, Jeffrey A. Hubbell, Yann Barrandon, Peter FreyAbstract:(A, B and C) Porcine urethelial holoclone, meroclone and paraclone cultures arising from a single urethral cell. (D and E) Growth curves of a porcine urothelial holoclone. (F, G) In vivo urothelial differentiation of porcine urethral urothelial holoclonal cell pellets implanted into the subcapsular space of the Swiss nu/nu mice, expressing cytokeratin 7, Uroplakin-2 and Uroplakin 3 (scale bars, 10 µm). Note the “micro-bladder” like structure.
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Human clonal urothelial cells arising from single ureteral cell.
2014Co-Authors: Hans M. Larsson, Francois Gorostidi, Jeffrey A. Hubbell, Yann Barrandon, Peter FreyAbstract:(A) Schematics showing the passage from the single selected cell to the in vivo implantated clonal cell pellet. (B,C and D) Human urothelial holoclone, meroclone and paraclone cultures arising from one single ureteral cell. (E and F) Growth curve of a human urothelial holoclone (G) In vivo urothelial differentiation of human ureteral urothelial holoclone pellets implanted into the subcapsular space of the Swiss nu/nu mice, expressing cytokeratin 7, Uroplakin-2 and Uroplakin 3 (scale bars, 10 µm). Note the “micro-bladder” like structure.
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Urothelial cell differentiation and “micro-bladder” formation in mice.
2014Co-Authors: Hans M. Larsson, Francois Gorostidi, Jeffrey A. Hubbell, Yann Barrandon, Peter FreyAbstract:(A and B) Hematoxylin & eosin (H&E) staining of an implanted mass-cultured, native porcine bladder urothelial cell pellet into the subcapsular space of a Swiss nu/nu mice kidney (A: scale bar 500 µm, B: scale bar 50 µm, urothelial bundle-like structure indicated with black star and epithelial “micro-bladder” like structure indicated with red star). (C–H) Immunohistochemistry of the implanted urothelial pellet forming urothelial bundle-like structures (C, E and G) and “micro-bladder” like structures (D, F and H) using antibodies against Uroplakin-2 (C and D), Uroplakin-3 (E and F) and Ki-67 (G and H) (scale bars, 10 µm).
Rubin, Mark A. - One of the best experts on this subject based on the ideXlab platform.
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Histological and immunohistochemical investigation of canine prostate carcinoma with identification of common intraductal carcinoma component
'Wiley', 2021Co-Authors: Brot Simone, Lothion‐roy Jennifer, Grau‐roma Llorenç, White Emily, Guscetti Franco, Rubin, Mark A., Mongan, Nigel P.Abstract:A limited number of species, including men and dogs, spontaneously develop prostate cancer (PC). The histological and molecular relevance of canine PC as a model for the disease in men remains controversial. To address this challenge, this study aimed to assess the histomorphology and expression of basal cell, urothelial and neuroendocrine markers [p63, high molecular weight cytokeratin (HMWCK), Uroplakin 3 (UPIII), neuron-specific enolase (NSE)] in canine PC (n = 41). Based on histomorphology, 10/41 (24%), 21/41 (51%) and 9/41 (22%) were classified as adenocarcinoma (AC), urothelial carcinoma (UC), and mixed carcinoma, respectively. Tumour inflammation was common, frequently severe [20/41 (49%)], and associated with neutering (p
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Histological and immunohistochemical investigation of canine prostate carcinoma with identification of common intraductal carcinoma component
'Wiley', 2021Co-Authors: De Brot Simone, Lothion‐roy Jennifer, Grau‐roma Llorenç, White Emily, Guscetti Franco, Rubin, Mark A., Mongan, Nigel P.Abstract:A limited number of species, including men and dogs, spontaneously develop prostate cancer (PC). The histological and molecular relevance of canine PC as a model for the disease in men remains controversial. To address this challenge, this study aimed to assess the histomorphology and expression of basal cell, urothelial and neuroendocrine markers [p63, high molecular weight cytokeratin (HMWCK), Uroplakin 3 (UPIII), neuron-specific enolase (NSE)] in canine PC (n = 41). Based on histomorphology, 10/41 (24%), 21/41 (51%) and 9/41 (22%) were classified as adenocarcinoma (AC), urothelial carcinoma (UC), and mixed carcinoma, respectively. Tumour inflammation was common, frequently severe [20/41 (49%)], and associated with neutering (p < .02) and urothelial differentiation (p < .02). Most (36/40, 90%) cancers contained only rare cells with basal cell marker expression or were negative. The expression of UPIII was absent or weak in the majority (33/38, 87%) of tumours, with moderate to strong staining in the remaining cases. NSE expression in PC was rare and limited to 2/14 (14%) cases. Tumour extension into benign ducts and glands was a common finding with presence in 17/39 (44%) of carcinomas with and without urothelial differentiation. In conclusion, we confirm that canine PC is characterized by absent or weak expression of basal cell and urothelial markers. Although rare, NSE expression, potentially indicating neuroendocrine differentiation, is reported for the first time in canine PCa. Intraductal carcinoma of the prostate with concurrent invasive PCa (IDCP-inv) is a frequent, not previously described, finding in dogs with PC
Guscetti Franco - One of the best experts on this subject based on the ideXlab platform.
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Histological and immunohistochemical investigation of canine prostate carcinoma with identification of common intraductal carcinoma component
'Wiley', 2021Co-Authors: Brot Simone, Lothion‐roy Jennifer, Grau‐roma Llorenç, White Emily, Guscetti Franco, Rubin, Mark A., Mongan, Nigel P.Abstract:A limited number of species, including men and dogs, spontaneously develop prostate cancer (PC). The histological and molecular relevance of canine PC as a model for the disease in men remains controversial. To address this challenge, this study aimed to assess the histomorphology and expression of basal cell, urothelial and neuroendocrine markers [p63, high molecular weight cytokeratin (HMWCK), Uroplakin 3 (UPIII), neuron-specific enolase (NSE)] in canine PC (n = 41). Based on histomorphology, 10/41 (24%), 21/41 (51%) and 9/41 (22%) were classified as adenocarcinoma (AC), urothelial carcinoma (UC), and mixed carcinoma, respectively. Tumour inflammation was common, frequently severe [20/41 (49%)], and associated with neutering (p
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Histological and immunohistochemical investigation of canine prostate carcinoma with identification of common intraductal carcinoma component
'Wiley', 2021Co-Authors: De Brot Simone, Lothion‐roy Jennifer, Grau‐roma Llorenç, White Emily, Guscetti Franco, Rubin, Mark A., Mongan, Nigel P.Abstract:A limited number of species, including men and dogs, spontaneously develop prostate cancer (PC). The histological and molecular relevance of canine PC as a model for the disease in men remains controversial. To address this challenge, this study aimed to assess the histomorphology and expression of basal cell, urothelial and neuroendocrine markers [p63, high molecular weight cytokeratin (HMWCK), Uroplakin 3 (UPIII), neuron-specific enolase (NSE)] in canine PC (n = 41). Based on histomorphology, 10/41 (24%), 21/41 (51%) and 9/41 (22%) were classified as adenocarcinoma (AC), urothelial carcinoma (UC), and mixed carcinoma, respectively. Tumour inflammation was common, frequently severe [20/41 (49%)], and associated with neutering (p < .02) and urothelial differentiation (p < .02). Most (36/40, 90%) cancers contained only rare cells with basal cell marker expression or were negative. The expression of UPIII was absent or weak in the majority (33/38, 87%) of tumours, with moderate to strong staining in the remaining cases. NSE expression in PC was rare and limited to 2/14 (14%) cases. Tumour extension into benign ducts and glands was a common finding with presence in 17/39 (44%) of carcinomas with and without urothelial differentiation. In conclusion, we confirm that canine PC is characterized by absent or weak expression of basal cell and urothelial markers. Although rare, NSE expression, potentially indicating neuroendocrine differentiation, is reported for the first time in canine PCa. Intraductal carcinoma of the prostate with concurrent invasive PCa (IDCP-inv) is a frequent, not previously described, finding in dogs with PC
White Emily - One of the best experts on this subject based on the ideXlab platform.
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Histological and immunohistochemical investigation of canine prostate carcinoma with identification of common intraductal carcinoma component
'Wiley', 2021Co-Authors: Brot Simone, Lothion‐roy Jennifer, Grau‐roma Llorenç, White Emily, Guscetti Franco, Rubin, Mark A., Mongan, Nigel P.Abstract:A limited number of species, including men and dogs, spontaneously develop prostate cancer (PC). The histological and molecular relevance of canine PC as a model for the disease in men remains controversial. To address this challenge, this study aimed to assess the histomorphology and expression of basal cell, urothelial and neuroendocrine markers [p63, high molecular weight cytokeratin (HMWCK), Uroplakin 3 (UPIII), neuron-specific enolase (NSE)] in canine PC (n = 41). Based on histomorphology, 10/41 (24%), 21/41 (51%) and 9/41 (22%) were classified as adenocarcinoma (AC), urothelial carcinoma (UC), and mixed carcinoma, respectively. Tumour inflammation was common, frequently severe [20/41 (49%)], and associated with neutering (p
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Histological and immunohistochemical investigation of canine prostate carcinoma with identification of common intraductal carcinoma component
'Wiley', 2021Co-Authors: De Brot Simone, Lothion‐roy Jennifer, Grau‐roma Llorenç, White Emily, Guscetti Franco, Rubin, Mark A., Mongan, Nigel P.Abstract:A limited number of species, including men and dogs, spontaneously develop prostate cancer (PC). The histological and molecular relevance of canine PC as a model for the disease in men remains controversial. To address this challenge, this study aimed to assess the histomorphology and expression of basal cell, urothelial and neuroendocrine markers [p63, high molecular weight cytokeratin (HMWCK), Uroplakin 3 (UPIII), neuron-specific enolase (NSE)] in canine PC (n = 41). Based on histomorphology, 10/41 (24%), 21/41 (51%) and 9/41 (22%) were classified as adenocarcinoma (AC), urothelial carcinoma (UC), and mixed carcinoma, respectively. Tumour inflammation was common, frequently severe [20/41 (49%)], and associated with neutering (p < .02) and urothelial differentiation (p < .02). Most (36/40, 90%) cancers contained only rare cells with basal cell marker expression or were negative. The expression of UPIII was absent or weak in the majority (33/38, 87%) of tumours, with moderate to strong staining in the remaining cases. NSE expression in PC was rare and limited to 2/14 (14%) cases. Tumour extension into benign ducts and glands was a common finding with presence in 17/39 (44%) of carcinomas with and without urothelial differentiation. In conclusion, we confirm that canine PC is characterized by absent or weak expression of basal cell and urothelial markers. Although rare, NSE expression, potentially indicating neuroendocrine differentiation, is reported for the first time in canine PCa. Intraductal carcinoma of the prostate with concurrent invasive PCa (IDCP-inv) is a frequent, not previously described, finding in dogs with PC