The Experts below are selected from a list of 12720 Experts worldwide ranked by ideXlab platform
Mats Hellstrom - One of the best experts on this subject based on the ideXlab platform.
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towards Uterus Tissue engineering a comparative study of sheep Uterus decellularisation
Molecular Human Reproduction, 2020Co-Authors: Tom Tristan Tiemann, Arvind Manikantan Padma, Edina Sehic, Henrik Backdahl, Mihai Oltean, Min Jong Song, Mats Brannstrom, Mats HellstromAbstract:Uterus Tissue engineering may dismantle limitations in current Uterus transplantation protocols. A uterine biomaterial populated with patient-derived cells could potentially serve as a graft to circumvent complicated surgery of live donors, immunosuppressive medication and rejection episodes. Repeated uterine bioengineering studies on rodents have shown promising results using decellularised scaffolds to restore fertility in a partially impaired Uterus and now mandate experiments on larger and more human-like animal models. The aim of the presented studies was therefore to establish adequate protocols for scaffold generation and prepare for future in vivo sheep Uterus bioengineering experiments. Three decellularisation protocols were developed using vascular perfusion through the uterine artery of whole sheep uteri obtained from slaughterhouse material. Decellularisation solutions used were based on 0.5% sodium dodecyl sulphate (Protocol 1) or 2% sodium deoxycholate (Protocol 2) or with a sequential perfusion of 2% sodium deoxycholate and 1% Triton X-100 (Protocol 3). The scaffolds were examined by histology, extracellular matrix quantification, evaluation of mechanical properties and the ability to support foetal sheep stem cells after recellularisation. We showed that a sheep Uterus can successfully be decellularised while maintaining a high integrity of the extracellular components. Uteri perfused with sodium deoxycholate (Protocol 2) were the most favourable treatment in our study based on quantifications. However, all scaffolds supported stem cells for 2 weeks in vitro and showed no cytotoxicity signs. Cells continued to express markers for proliferation and maintained their undifferentiated phenotype. Hence, this study reports three valuable decellularisation protocols for future in vivo sheep Uterus bioengineering experiments.
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protocols for rat Uterus isolation and decellularization applications for Uterus Tissue engineering and 3d cell culturing
Methods of Molecular Biology, 2017Co-Authors: Arvind Manikantan Padma, Tom Tristan Tiemann, Min Jong Song, Ahmed Baker Alshaikh, Randa Akouri, Mats HellstromAbstract:Sophisticated culturing conditions are required to grow cells in a three-dimensional (3D) environment. Cells then require a type of scaffold rich in proteins, growth factors, and signaling molecules that simulates their natural environment. Tissues from all species of animals have an organ-specific extracellular matrix (ECM) structure that plays a key role in cell proliferation and migration. Hence, the scaffold composition plays a significant role for any successful 3D cell culturing system. We developed a whole rat Uterus ECM scaffold by the perfusion of detergents and ionic solutions through the vascular system of an isolated normal rat Uterus in a process termed "decellularization." The generated rat Uterus scaffolds consist of a cell-free ECM structure similar to that of the normal rat Uterus, and are thus excellent platforms on to which new cells can be added. Rat Uterus 3D cell culturing systems based on these scaffolds could become valuable to decidual differentiation- and embryo implantation studies, or for investigating invasion mechanisms of endometrial cancer cells. They could also be used for the creation of Tissue engineered uterine Tissue, for partial or whole organogenesis developed for transplantation applications to treat absolute uterine infertility. This is a condition affecting about 1 in 500 women, and is only treatable by a Uterus transplantation. This article provides valuable troubleshooting notes and describes in detail how to generate rat Uterus scaffolds, including the delicate surgery required to isolate the Uterus with an intact vascular tree which facilitates vascular perfusion and re-transplantation.
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towards the development of a bioengineered Uterus comparison of different protocols for rat Uterus decellularization
Acta Biomaterialia, 2014Co-Authors: Mats Hellstrom, Henrik Backdahl, R R Elakouri, Carina Sihlbom, Brittmarie Olsson, J Lengqvist, Bengt R Johansson, Michael Olausson, Suchitra Sumitranholgersson, Mats BrannstromAbstract:Uterus transplantation (UTx) may be the only possible curative treatment for absolute uterine factor infertility, which affects 1 in every 500 females of fertile age. We recently presented the 6-month results from the first clinical UTx trial, describing nine live-donor procedures. This routine involves complicated surgery and requires potentially harmful immune suppression to prevent rejection. However, Tissue engineering applications using biomaterials and stem cells may replace the need for a live donor, and could prevent the required immunosuppressive treatment. To investigate the basic aspects of this, we developed a novel whole-Uterus scaffold design for Uterus Tissue engineering experiments in the rat. Decellularization was achieved by perfusion of detergents and ionic solutions. The remaining matrix and its biochemical and mechanical properties were quantitatively compared from using three different protocols. The constructs were further compared with native Uterus Tissue composition. Perfusion with Triton X-100/dimethyl sulfoxide/H2O led to a compact, weaker scaffold that showed evidence of a compromised matrix organization. Sodium deoxycholate/dH2O perfusion gave rise to a porous scaffold that structurally and mechanically resembled native Uterus better. An innovative combination of two proteomic analyses revealed higher fibronectin and versican content in these porous scaffolds, which may explain the improved scaffold organization. Together with other important protocol-dependent differences, our results can contribute to the development of improved decellularization protocols for assorted organs. Furthermore, our study shows the first available data on decellularized whole Uterus, and creates new opportunities for numerous in vitro and in vivo whole-Uterus Tissue engineering applications.
Mats Brannstrom - One of the best experts on this subject based on the ideXlab platform.
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towards Uterus Tissue engineering a comparative study of sheep Uterus decellularisation
Molecular Human Reproduction, 2020Co-Authors: Tom Tristan Tiemann, Arvind Manikantan Padma, Edina Sehic, Henrik Backdahl, Mihai Oltean, Min Jong Song, Mats Brannstrom, Mats HellstromAbstract:Uterus Tissue engineering may dismantle limitations in current Uterus transplantation protocols. A uterine biomaterial populated with patient-derived cells could potentially serve as a graft to circumvent complicated surgery of live donors, immunosuppressive medication and rejection episodes. Repeated uterine bioengineering studies on rodents have shown promising results using decellularised scaffolds to restore fertility in a partially impaired Uterus and now mandate experiments on larger and more human-like animal models. The aim of the presented studies was therefore to establish adequate protocols for scaffold generation and prepare for future in vivo sheep Uterus bioengineering experiments. Three decellularisation protocols were developed using vascular perfusion through the uterine artery of whole sheep uteri obtained from slaughterhouse material. Decellularisation solutions used were based on 0.5% sodium dodecyl sulphate (Protocol 1) or 2% sodium deoxycholate (Protocol 2) or with a sequential perfusion of 2% sodium deoxycholate and 1% Triton X-100 (Protocol 3). The scaffolds were examined by histology, extracellular matrix quantification, evaluation of mechanical properties and the ability to support foetal sheep stem cells after recellularisation. We showed that a sheep Uterus can successfully be decellularised while maintaining a high integrity of the extracellular components. Uteri perfused with sodium deoxycholate (Protocol 2) were the most favourable treatment in our study based on quantifications. However, all scaffolds supported stem cells for 2 weeks in vitro and showed no cytotoxicity signs. Cells continued to express markers for proliferation and maintained their undifferentiated phenotype. Hence, this study reports three valuable decellularisation protocols for future in vivo sheep Uterus bioengineering experiments.
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towards the development of a bioengineered Uterus comparison of different protocols for rat Uterus decellularization
Acta Biomaterialia, 2014Co-Authors: Mats Hellstrom, Henrik Backdahl, R R Elakouri, Carina Sihlbom, Brittmarie Olsson, J Lengqvist, Bengt R Johansson, Michael Olausson, Suchitra Sumitranholgersson, Mats BrannstromAbstract:Uterus transplantation (UTx) may be the only possible curative treatment for absolute uterine factor infertility, which affects 1 in every 500 females of fertile age. We recently presented the 6-month results from the first clinical UTx trial, describing nine live-donor procedures. This routine involves complicated surgery and requires potentially harmful immune suppression to prevent rejection. However, Tissue engineering applications using biomaterials and stem cells may replace the need for a live donor, and could prevent the required immunosuppressive treatment. To investigate the basic aspects of this, we developed a novel whole-Uterus scaffold design for Uterus Tissue engineering experiments in the rat. Decellularization was achieved by perfusion of detergents and ionic solutions. The remaining matrix and its biochemical and mechanical properties were quantitatively compared from using three different protocols. The constructs were further compared with native Uterus Tissue composition. Perfusion with Triton X-100/dimethyl sulfoxide/H2O led to a compact, weaker scaffold that showed evidence of a compromised matrix organization. Sodium deoxycholate/dH2O perfusion gave rise to a porous scaffold that structurally and mechanically resembled native Uterus better. An innovative combination of two proteomic analyses revealed higher fibronectin and versican content in these porous scaffolds, which may explain the improved scaffold organization. Together with other important protocol-dependent differences, our results can contribute to the development of improved decellularization protocols for assorted organs. Furthermore, our study shows the first available data on decellularized whole Uterus, and creates new opportunities for numerous in vitro and in vivo whole-Uterus Tissue engineering applications.
Henrik Backdahl - One of the best experts on this subject based on the ideXlab platform.
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towards Uterus Tissue engineering a comparative study of sheep Uterus decellularisation
Molecular Human Reproduction, 2020Co-Authors: Tom Tristan Tiemann, Arvind Manikantan Padma, Edina Sehic, Henrik Backdahl, Mihai Oltean, Min Jong Song, Mats Brannstrom, Mats HellstromAbstract:Uterus Tissue engineering may dismantle limitations in current Uterus transplantation protocols. A uterine biomaterial populated with patient-derived cells could potentially serve as a graft to circumvent complicated surgery of live donors, immunosuppressive medication and rejection episodes. Repeated uterine bioengineering studies on rodents have shown promising results using decellularised scaffolds to restore fertility in a partially impaired Uterus and now mandate experiments on larger and more human-like animal models. The aim of the presented studies was therefore to establish adequate protocols for scaffold generation and prepare for future in vivo sheep Uterus bioengineering experiments. Three decellularisation protocols were developed using vascular perfusion through the uterine artery of whole sheep uteri obtained from slaughterhouse material. Decellularisation solutions used were based on 0.5% sodium dodecyl sulphate (Protocol 1) or 2% sodium deoxycholate (Protocol 2) or with a sequential perfusion of 2% sodium deoxycholate and 1% Triton X-100 (Protocol 3). The scaffolds were examined by histology, extracellular matrix quantification, evaluation of mechanical properties and the ability to support foetal sheep stem cells after recellularisation. We showed that a sheep Uterus can successfully be decellularised while maintaining a high integrity of the extracellular components. Uteri perfused with sodium deoxycholate (Protocol 2) were the most favourable treatment in our study based on quantifications. However, all scaffolds supported stem cells for 2 weeks in vitro and showed no cytotoxicity signs. Cells continued to express markers for proliferation and maintained their undifferentiated phenotype. Hence, this study reports three valuable decellularisation protocols for future in vivo sheep Uterus bioengineering experiments.
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towards the development of a bioengineered Uterus comparison of different protocols for rat Uterus decellularization
Acta Biomaterialia, 2014Co-Authors: Mats Hellstrom, Henrik Backdahl, R R Elakouri, Carina Sihlbom, Brittmarie Olsson, J Lengqvist, Bengt R Johansson, Michael Olausson, Suchitra Sumitranholgersson, Mats BrannstromAbstract:Uterus transplantation (UTx) may be the only possible curative treatment for absolute uterine factor infertility, which affects 1 in every 500 females of fertile age. We recently presented the 6-month results from the first clinical UTx trial, describing nine live-donor procedures. This routine involves complicated surgery and requires potentially harmful immune suppression to prevent rejection. However, Tissue engineering applications using biomaterials and stem cells may replace the need for a live donor, and could prevent the required immunosuppressive treatment. To investigate the basic aspects of this, we developed a novel whole-Uterus scaffold design for Uterus Tissue engineering experiments in the rat. Decellularization was achieved by perfusion of detergents and ionic solutions. The remaining matrix and its biochemical and mechanical properties were quantitatively compared from using three different protocols. The constructs were further compared with native Uterus Tissue composition. Perfusion with Triton X-100/dimethyl sulfoxide/H2O led to a compact, weaker scaffold that showed evidence of a compromised matrix organization. Sodium deoxycholate/dH2O perfusion gave rise to a porous scaffold that structurally and mechanically resembled native Uterus better. An innovative combination of two proteomic analyses revealed higher fibronectin and versican content in these porous scaffolds, which may explain the improved scaffold organization. Together with other important protocol-dependent differences, our results can contribute to the development of improved decellularization protocols for assorted organs. Furthermore, our study shows the first available data on decellularized whole Uterus, and creates new opportunities for numerous in vitro and in vivo whole-Uterus Tissue engineering applications.
Min Jong Song - One of the best experts on this subject based on the ideXlab platform.
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towards Uterus Tissue engineering a comparative study of sheep Uterus decellularisation
Molecular Human Reproduction, 2020Co-Authors: Tom Tristan Tiemann, Arvind Manikantan Padma, Edina Sehic, Henrik Backdahl, Mihai Oltean, Min Jong Song, Mats Brannstrom, Mats HellstromAbstract:Uterus Tissue engineering may dismantle limitations in current Uterus transplantation protocols. A uterine biomaterial populated with patient-derived cells could potentially serve as a graft to circumvent complicated surgery of live donors, immunosuppressive medication and rejection episodes. Repeated uterine bioengineering studies on rodents have shown promising results using decellularised scaffolds to restore fertility in a partially impaired Uterus and now mandate experiments on larger and more human-like animal models. The aim of the presented studies was therefore to establish adequate protocols for scaffold generation and prepare for future in vivo sheep Uterus bioengineering experiments. Three decellularisation protocols were developed using vascular perfusion through the uterine artery of whole sheep uteri obtained from slaughterhouse material. Decellularisation solutions used were based on 0.5% sodium dodecyl sulphate (Protocol 1) or 2% sodium deoxycholate (Protocol 2) or with a sequential perfusion of 2% sodium deoxycholate and 1% Triton X-100 (Protocol 3). The scaffolds were examined by histology, extracellular matrix quantification, evaluation of mechanical properties and the ability to support foetal sheep stem cells after recellularisation. We showed that a sheep Uterus can successfully be decellularised while maintaining a high integrity of the extracellular components. Uteri perfused with sodium deoxycholate (Protocol 2) were the most favourable treatment in our study based on quantifications. However, all scaffolds supported stem cells for 2 weeks in vitro and showed no cytotoxicity signs. Cells continued to express markers for proliferation and maintained their undifferentiated phenotype. Hence, this study reports three valuable decellularisation protocols for future in vivo sheep Uterus bioengineering experiments.
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protocols for rat Uterus isolation and decellularization applications for Uterus Tissue engineering and 3d cell culturing
Methods of Molecular Biology, 2017Co-Authors: Arvind Manikantan Padma, Tom Tristan Tiemann, Min Jong Song, Ahmed Baker Alshaikh, Randa Akouri, Mats HellstromAbstract:Sophisticated culturing conditions are required to grow cells in a three-dimensional (3D) environment. Cells then require a type of scaffold rich in proteins, growth factors, and signaling molecules that simulates their natural environment. Tissues from all species of animals have an organ-specific extracellular matrix (ECM) structure that plays a key role in cell proliferation and migration. Hence, the scaffold composition plays a significant role for any successful 3D cell culturing system. We developed a whole rat Uterus ECM scaffold by the perfusion of detergents and ionic solutions through the vascular system of an isolated normal rat Uterus in a process termed "decellularization." The generated rat Uterus scaffolds consist of a cell-free ECM structure similar to that of the normal rat Uterus, and are thus excellent platforms on to which new cells can be added. Rat Uterus 3D cell culturing systems based on these scaffolds could become valuable to decidual differentiation- and embryo implantation studies, or for investigating invasion mechanisms of endometrial cancer cells. They could also be used for the creation of Tissue engineered uterine Tissue, for partial or whole organogenesis developed for transplantation applications to treat absolute uterine infertility. This is a condition affecting about 1 in 500 women, and is only treatable by a Uterus transplantation. This article provides valuable troubleshooting notes and describes in detail how to generate rat Uterus scaffolds, including the delicate surgery required to isolate the Uterus with an intact vascular tree which facilitates vascular perfusion and re-transplantation.
Tom Tristan Tiemann - One of the best experts on this subject based on the ideXlab platform.
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towards Uterus Tissue engineering a comparative study of sheep Uterus decellularisation
Molecular Human Reproduction, 2020Co-Authors: Tom Tristan Tiemann, Arvind Manikantan Padma, Edina Sehic, Henrik Backdahl, Mihai Oltean, Min Jong Song, Mats Brannstrom, Mats HellstromAbstract:Uterus Tissue engineering may dismantle limitations in current Uterus transplantation protocols. A uterine biomaterial populated with patient-derived cells could potentially serve as a graft to circumvent complicated surgery of live donors, immunosuppressive medication and rejection episodes. Repeated uterine bioengineering studies on rodents have shown promising results using decellularised scaffolds to restore fertility in a partially impaired Uterus and now mandate experiments on larger and more human-like animal models. The aim of the presented studies was therefore to establish adequate protocols for scaffold generation and prepare for future in vivo sheep Uterus bioengineering experiments. Three decellularisation protocols were developed using vascular perfusion through the uterine artery of whole sheep uteri obtained from slaughterhouse material. Decellularisation solutions used were based on 0.5% sodium dodecyl sulphate (Protocol 1) or 2% sodium deoxycholate (Protocol 2) or with a sequential perfusion of 2% sodium deoxycholate and 1% Triton X-100 (Protocol 3). The scaffolds were examined by histology, extracellular matrix quantification, evaluation of mechanical properties and the ability to support foetal sheep stem cells after recellularisation. We showed that a sheep Uterus can successfully be decellularised while maintaining a high integrity of the extracellular components. Uteri perfused with sodium deoxycholate (Protocol 2) were the most favourable treatment in our study based on quantifications. However, all scaffolds supported stem cells for 2 weeks in vitro and showed no cytotoxicity signs. Cells continued to express markers for proliferation and maintained their undifferentiated phenotype. Hence, this study reports three valuable decellularisation protocols for future in vivo sheep Uterus bioengineering experiments.
-
protocols for rat Uterus isolation and decellularization applications for Uterus Tissue engineering and 3d cell culturing
Methods of Molecular Biology, 2017Co-Authors: Arvind Manikantan Padma, Tom Tristan Tiemann, Min Jong Song, Ahmed Baker Alshaikh, Randa Akouri, Mats HellstromAbstract:Sophisticated culturing conditions are required to grow cells in a three-dimensional (3D) environment. Cells then require a type of scaffold rich in proteins, growth factors, and signaling molecules that simulates their natural environment. Tissues from all species of animals have an organ-specific extracellular matrix (ECM) structure that plays a key role in cell proliferation and migration. Hence, the scaffold composition plays a significant role for any successful 3D cell culturing system. We developed a whole rat Uterus ECM scaffold by the perfusion of detergents and ionic solutions through the vascular system of an isolated normal rat Uterus in a process termed "decellularization." The generated rat Uterus scaffolds consist of a cell-free ECM structure similar to that of the normal rat Uterus, and are thus excellent platforms on to which new cells can be added. Rat Uterus 3D cell culturing systems based on these scaffolds could become valuable to decidual differentiation- and embryo implantation studies, or for investigating invasion mechanisms of endometrial cancer cells. They could also be used for the creation of Tissue engineered uterine Tissue, for partial or whole organogenesis developed for transplantation applications to treat absolute uterine infertility. This is a condition affecting about 1 in 500 women, and is only treatable by a Uterus transplantation. This article provides valuable troubleshooting notes and describes in detail how to generate rat Uterus scaffolds, including the delicate surgery required to isolate the Uterus with an intact vascular tree which facilitates vascular perfusion and re-transplantation.
