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Francis A. Drummond - One of the best experts on this subject based on the ideXlab platform.
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Analysis of Pollination Services Provided by Wild and Managed Bees (Apoidea) in Wild Blueberry (Vaccinium angustifolium Aiton) Production in Maine, USA, with a Literature Review
Agronomy, 2020Co-Authors: Sara L. Bushmann, Francis A. DrummondAbstract:Maine is the largest producer of wild blueberry (Vaccinium angustifolium Aiton) in the United States. Pollination comes from combinations of honey bees (Apis mellifera (L.)), commercial bumble bees (Bombus impatiens Cresson), and wild bees. This study addresses (1) previous research addressing wild-blueberry pollination, (2) effects of wild-bee and honey-bee activity densities on fruit set, yield, and crop value, (3) the economic value of wild-bee communities, and (4) economic consequences of pollinator loss. Bee communities were sampled in 40 fields over three years (2010–2012) and bee activity densities were estimated for bumble bees, honey bees, and other wild bees. These data were applied to an economic model to estimate the value of bee taxa. Bumble bees and honey bees predicted fruit set and reduced its spatial heterogeneity. Other wild bees were not significant predictors of fruit set. Yield was predicted by fruit set and field size, but not pest management tactics. Our analysis showed that disruption in supply of honey bees would result in nearly a 30% decrease in crop yield, buffered in part by wild bees that provide “background” levels of pollination. Honey-bee stocking density and, thus, the activity density of honey bees was greater in larger fields, but not for wild bees. Therefore, a decrease in crop yield would be greater than 30% for large fields due to the proportionally greater investment in honey bees in large fields and a relatively lower contribution by wild bees.
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The Ecology of Autogamy in Wild Blueberry (Vaccinium angustifolium Aiton): Does the Early Clone Get the Bee?
Agronomy, 2020Co-Authors: Francis A. Drummond, Lisa J RowlandAbstract:Wild blueberry, Vaccinium angustifolium Aiton, for the most part requires cross-pollination. However, there is a continuum across a gradient from zero to 100% in self-compatibility. We previously found by sampling many fields that 20–25% of clones during bloom have high levels of self-compatibility (≥50%). In 2009–2011, and 2015 we studied the ecology of self-pollination in wild blueberry, specifically its phenology and bee recruitment and subsequent bee density on bloom. We found that highly self-compatible clones were predominantly early blooming genotypes in the wild blueberry population. On average, fruit set and berry weight were highest in self-compatible genotypes. The bumble bee community (queens only early in the spring) was characterized by bees that spent large amounts of time foraging in self-compatible plant patches that comprised only a small proportion of the blueberry field, the highest density in the beginning of bloom when most genotypes in bloom were self-compatible. As bloom proceeded in the spring, more plants were in bloom and thus more land area was occupied by blooming plants. The absolute density of bumble bee queens per m2 declined, as a dilution effect, and this probably resulted in lower fruit set throughout the field.
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Spotted-Wing Drosophila (Diptera: Drosophilidae) Adult Movement, Activity, and Oviposition Behavior in Maine Wild Blueberry (Vaccinium angustifolium; Ericales: Ericaceae)
Journal of economic entomology, 2019Co-Authors: Francis A. Drummond, Elissa Ballman, Judith A CollinsAbstract:Over a period of 5 yr (2012-2016), we conducted laboratory and field studies on activity, movement, and response to trap placement of adult Drosophila suzukii (Matsumura) in wild blueberry, Vaccinium angustifolium Aiton, fields in Maine. When measuring temporal patterns in fruit infestation, we found that D. suzukii females are most active in the morning and that they are 10 times more likely to lay eggs in blueberries at the top of the plant canopy compared with berries located in the lower part of the bush. Flies were found to be more abundant in fruit-bearing (crop) fields compared with pruned (vegetative) fields based on trap capture of adults. They are also most abundant along edges of fields compared with interiors. Trap efficiency is significantly better in traps 1.2 m above the ground and above the crop canopy of this low-growing crop plant than within the crop canopy. Three experiments involving the marking of laboratory-reared flies with fluorescent marker, their release, and capture with traps along a grid in fields suggest that: 1) fluorescent markers do not affect the distance moved of marked flies, 2) dispersal rates are not different between sexes, 3) there is little difference in the dispersal pattern through pruned fields and fruit-bearing fields, and 4) flies disperse at a low rate of 0.1-30 m per day, with an average of 5 m per day, but that long-distance dispersal over 1-2 km is feasible based on statistical model extrapolation.
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The Blueberry Gall Midge (Diptera: Cecidomyiidae): A Recent Pest of Wild Blueberry (Vaccinium angustifolium; Ericales: Ericaceae) and Its Impact on Potential Yield.
Journal of economic entomology, 2019Co-Authors: Judith A Collins, Francis A. DrummondAbstract:Blueberry gall midge, Dasineura oxycoccana (Johnson) (Diptera: Cecidomyiidae), is an emerging pest on wild blueberry, Vaccinium angustifolium Aiton (Ericales: Ericaceae). The purpose of this study was to document the population increase of blueberry gall midge on this crop in Maine since its discovery in 2003 until 2018. Dasineura oxycoccana appears to have three generations during the prune cycle in Maine wild blueberry, although this may vary among years. Prune fields have higher infestation rates than crop fields, most likely due to the greater abundance of susceptible leaf tissue. Production system does affect infestation rates. Fields managed under a high input system exhibit lower gall midge infestation than low or medium input fields. Field infestation rates in organic fields were intermediate to high input and low and medium input fields. In seven trials conducted between 2010 and 2017, D. oxycoccana infestation of stems resulted in significantly fewer flower-bud clusters developed at the end of the prune year in four of seven trials and significantly fewer viable flowers during bloom in the crop year in four of seven trials. Two of the seven trials resulted in significantly more flowers on infested stems than noninfested stems, evidence that in some years D. oxycoccana infestation may stimulate flower-bud production, resulting in an increase in potential yield. We provide an optimal sampling plan for D. oxycoccana infestation sampling and economic thresholds for three levels of production (yield levels) and three expected prices that growers might receive.
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grid set match an agent based simulation model predicts fruit set for the lowbush blueberry Vaccinium angustifolium agroecosystem
Ecological Modelling, 2017Co-Authors: Alex W Bajcz, David E Hiebeler, Francis A. DrummondAbstract:Abstract Fruit set, the proportion of flowers whose ovaries successfully bear fruit, is the product of dozens of processes, many of which are difficult to study and manipulate in situ . To establish which of these processes are most important in the lowbush blueberry ( Vaccinium angustifolium Aiton; Ericaceae) agroecosystem, an agent-based simulation model, Grid-Set-Match , with temporal and spatial elements was constructed using the R software package. Pollination ecology data from this system were used to parameterize the model. Then, results from 1,000 iterations of the model were compared to field fruit set data from a survey of 162 lowbush blueberry fields from 1993 to 2015 as well as from a field study conducted from 2013 to 2015. Predicted fruit set for an average lowbush blueberry field was 0.366 according to Grid-Set-Match . Based on currently available field data, this estimate appears realistic, although it may be a slight underestimate in part because variability in pollinator densities between real fields is higher than the model accounted for. A multiple regression model indicated that, across a sample of clones created by Grid-Set-Match under default parameter settings, fruit set declined significantly as the average genetic load of a clone’s neighbors increased and as self-pollination rate increased, with the latter effect being ∼20% stronger than the former. As such, future research should be directed towards understanding and better managing factors that may influence rates of self- vs. cross-pollination in this system, such as pollen carryover, intra-hive pollen transfer, and pollinator flight distances between consecutively visited flowers.
Shannon A. Bainbridge - One of the best experts on this subject based on the ideXlab platform.
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Vaccinium angustifolium lowbush blueberry leaf extract increases extravillous trophoblast cell migration and invasion in vitro
Phytotherapy Research, 2018Co-Authors: Christina Ly, Jonathan Ferrier, Jeremiah Gaudet, John T. Arnason, Andrée Gruslin, Shannon A. Bainbridge, Julien YockelllelievreAbstract:: Perturbations to extravillous trophoblast (EVT) cell migration and invasion are associated with the development of placenta-mediated diseases. Phytochemicals found in the lowbush blueberry plant (Vaccinium angustifolium) have been shown to influence cell migration and invasion in models of tumorigenesis and noncancerous, healthy cells, however never in EVT cells. We hypothesized that the phenolic compounds present in V. angustifolium leaf extract promote trophoblast migration and invasion. Using the HTR-8/SVneo human EVT cell line and Boyden chamber assays, the influence of V. angustifolium leaf extract (0 to 2 × 104 ng/ml) on trophoblast cell migration (n = 4) and invasion (n = 4) was determined. Cellular proliferation and viability were assessed using immunoreactivity to Ki67 (n = 3) and trypan blue exclusion assays (n = 3), respectively. At 20 ng/ml, V. angustifolium leaf extract increased HTR-8/SVneo cell migration and invasion (p < .01) and did not affect cell proliferation or viability. Chlorogenic acid was identified as a major phenolic compound of the leaf extract and the most active compound. Evidence from Western blot analysis (n = 3) suggests that the effects of the leaf extract and chlorogenic acid on trophoblast migration and invasion are mediated through an adenosine monophosphate-activated protein (AMP) kinase-dependent mechanism. Further investigations examining the potential therapeutic applications of this natural health product extract and its major chemical compounds in the context of placenta-mediated diseases are warranted.
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Vaccinium angustifolium (lowbush blueberry) leaf extract increases extravillous trophoblast cell migration and invasion in vitro.
Phytotherapy research : PTR, 2018Co-Authors: Jonathan Ferrier, Jeremiah Gaudet, Julien Yockell-lelièvre, John T. Arnason, Andrée Gruslin, Shannon A. BainbridgeAbstract:Perturbations to extravillous trophoblast (EVT) cell migration and invasion are associated with the development of placenta-mediated diseases. Phytochemicals found in the lowbush blueberry plant (Vaccinium angustifolium) have been shown to influence cell migration and invasion in models of tumorigenesis and noncancerous, healthy cells, however never in EVT cells. We hypothesized that the phenolic compounds present in V. angustifolium leaf extract promote trophoblast migration and invasion. Using the HTR-8/SVneo human EVT cell line and Boyden chamber assays, the influence of V. angustifolium leaf extract (0 to 2 × 104 ng/ml) on trophoblast cell migration (n = 4) and invasion (n = 4) was determined. Cellular proliferation and viability were assessed using immunoreactivity to Ki67 (n = 3) and trypan blue exclusion assays (n = 3), respectively. At 20 ng/ml, V. angustifolium leaf extract increased HTR-8/SVneo cell migration and invasion (p
John T. Arnason - One of the best experts on this subject based on the ideXlab platform.
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Vaccinium angustifolium lowbush blueberry leaf extract increases extravillous trophoblast cell migration and invasion in vitro
Phytotherapy Research, 2018Co-Authors: Christina Ly, Jonathan Ferrier, Jeremiah Gaudet, John T. Arnason, Andrée Gruslin, Shannon A. Bainbridge, Julien YockelllelievreAbstract:: Perturbations to extravillous trophoblast (EVT) cell migration and invasion are associated with the development of placenta-mediated diseases. Phytochemicals found in the lowbush blueberry plant (Vaccinium angustifolium) have been shown to influence cell migration and invasion in models of tumorigenesis and noncancerous, healthy cells, however never in EVT cells. We hypothesized that the phenolic compounds present in V. angustifolium leaf extract promote trophoblast migration and invasion. Using the HTR-8/SVneo human EVT cell line and Boyden chamber assays, the influence of V. angustifolium leaf extract (0 to 2 × 104 ng/ml) on trophoblast cell migration (n = 4) and invasion (n = 4) was determined. Cellular proliferation and viability were assessed using immunoreactivity to Ki67 (n = 3) and trypan blue exclusion assays (n = 3), respectively. At 20 ng/ml, V. angustifolium leaf extract increased HTR-8/SVneo cell migration and invasion (p < .01) and did not affect cell proliferation or viability. Chlorogenic acid was identified as a major phenolic compound of the leaf extract and the most active compound. Evidence from Western blot analysis (n = 3) suggests that the effects of the leaf extract and chlorogenic acid on trophoblast migration and invasion are mediated through an adenosine monophosphate-activated protein (AMP) kinase-dependent mechanism. Further investigations examining the potential therapeutic applications of this natural health product extract and its major chemical compounds in the context of placenta-mediated diseases are warranted.
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Vaccinium angustifolium (lowbush blueberry) leaf extract increases extravillous trophoblast cell migration and invasion in vitro.
Phytotherapy research : PTR, 2018Co-Authors: Jonathan Ferrier, Jeremiah Gaudet, Julien Yockell-lelièvre, John T. Arnason, Andrée Gruslin, Shannon A. BainbridgeAbstract:Perturbations to extravillous trophoblast (EVT) cell migration and invasion are associated with the development of placenta-mediated diseases. Phytochemicals found in the lowbush blueberry plant (Vaccinium angustifolium) have been shown to influence cell migration and invasion in models of tumorigenesis and noncancerous, healthy cells, however never in EVT cells. We hypothesized that the phenolic compounds present in V. angustifolium leaf extract promote trophoblast migration and invasion. Using the HTR-8/SVneo human EVT cell line and Boyden chamber assays, the influence of V. angustifolium leaf extract (0 to 2 × 104 ng/ml) on trophoblast cell migration (n = 4) and invasion (n = 4) was determined. Cellular proliferation and viability were assessed using immunoreactivity to Ki67 (n = 3) and trypan blue exclusion assays (n = 3), respectively. At 20 ng/ml, V. angustifolium leaf extract increased HTR-8/SVneo cell migration and invasion (p
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quantification of chlorogenic acid and hyperoside directly from crude blueberry Vaccinium angustifolium leaf extract by nmr spectroscopy analysis single laboratory validation
Journal of AOAC International, 2012Co-Authors: Joshua M Hicks, Jonathan Ferrier, John T. Arnason, Ammar Saleem, Asim Muhammad, Alain Cuerrier, Kimberly L ColsonAbstract:A single-laboratory-validated NMR spectroscopy method was established for determining the quantity of chlorogenic acid and hyperoside from crude extract material of blueberry leaves of the species Vaccinium angustifolium var. laevifolium House. The calibration curve of chlorogenic acid showed a highly linear regression, R = 0.99998. NMR spectroscopy identification and quantification of the constituents directly from the mixture, within the error of HPLC-diode array detector analysis, were determined as 7.53 mM chlorogenic acid (64.0 mg chlorogenic acid/g powdered leaf) and 0.77 mM hyperoside (8.58 mg hyperoside/g powdered leaf). The LOD was calculated to be 0.01 mM and the LOQ 0.01 mM by the 9 min and 13 s NMR spectroscopy experiment utilized. The assay showed no significant interference from different field strengths, extraction mesh size, gravimetric scale precision, NMR spectroscopy tube type, pulse program, amount of starting dry material, or day-to-day operation. The robustness of NMR spectroscopy as a means of definitively monitoring chlorogenic acid and hyperoside content directly from crude extracts was demonstrated by Youden statistical analysis.
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seasonal phytochemical variation of anti glycation principles in lowbush blueberry Vaccinium angustifolium
Planta Medica, 2009Co-Authors: Kristina L Mcintyre, Cory S Harris, Ammar Saleem, Louisphilippe Beaulieu, Chieu Anh Ta, Pierre S Haddad, John T. ArnasonAbstract:: Diabetic hyperglycaemia promotes the production of advanced glycation end-products (AGEs), which play a significant role in the development of complications associated with type 2 diabetes mellitus. Vaccinium angustifolium, a medicinal plant used for the treatment of diabetes, produces a variety of phenolic metabolites with putative anti-diabetic activities. To assess optimal cultivation time, seasonal changes in the concentration of six phenolic compounds in leaves and twelve compounds in stems were examined using HPLC-DAD and examined in relation to seasonal changes in AGE inhibition activity, assessed with a fluorescence-based assay. A seasonal decline occurred in the concentration of chlorogenic acid, rutin, and quercetin 3-arabinoside in leaves and chlorogenic acid in stems. The concentration of (+)-catechin, and (-)-epicatechin in stems declined within two weeks before rising and fluctuating insignificantly. AGE inhibition activity of leaves was significantly greater at the final compared to the initial collection date whereas the activity of stems did not change significantly. Relative to the leaf extract, the stem was a more potent inhibitor of AGE formation, which could be a result of the unique phytochemistry of stems. Together, these results revealed significant seasonal variation in the phenolic profile and anti-glycation effects of V. angustifolium extracts and indicated late summer as the collection time yielding optimal activity.
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a single hplc pad apci ms method for the quantitative comparison of phenolic compounds found in leaf stem root and fruit extracts of Vaccinium angustifolium
Phytochemical Analysis, 2007Co-Authors: Cory S Harris, Ammar Saleem, Pierre S Haddad, Louis C Martineau, Andrew J. Burt, Steffany A. L. Bennett, John T. ArnasonAbstract:A method was developed for the analysis of Vaccinium angustifolium Ait. (Lowbush blueberry), which is a widely used natural health product, particularly for the treatment of diabetic symptoms. While the anthocyanin content of the fruit has been well characterized, the chemistry of the vegetative parts used in supportive therapy for diabetes has been largely ignored. Using a metabolomics-based approach for compound identification with an emphasis on phenolic metabolites, a single HPLC-PAD-APCI/MS method was developed for the separation and quantitation of the major metabolites found in the 95% ethanol extracts of leaf, stem, root and fruit. The leaf extract contained high concentrations of chlorogenic acid (∼100 µg/mg extract) and a variety of quercetin glycosides that were also detected in the fruit and stem extracts. Flavan-3-ol monomers (+)-catechin and (−) -epicatechin were found in all plant parts but their procyanidin dimers were exclusively identified in the stem and root. The accuracy and precision of the presented method were corroborated by low intra- and inter-day variations in quantitative results in all plant part extracts. Further validation of the extraction and analytical protocols focused on identified compounds with reputed anti-diabetic activity, revealing recoveries greater than 80% and detection limits of 0.12–2.73 µg/mL. Copyright © 2007 John Wiley & Sons, Ltd.
Mohamed Hijri - One of the best experts on this subject based on the ideXlab platform.
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Into the wild blueberry ( Vaccinium angustifolium ) rhizosphere microbiota
Environmental Microbiology, 2020Co-Authors: Simon Morvan, Hacène Meglouli, Anissa Lounès‐hadj Sahraoui, Mohamed HijriAbstract:The ability of wild blueberries to adapt to their harsh environment is believed to be closely related to their symbiosis with ericoid mycorrhizal fungi, which produce enzymes capable of organic matter mineralization. Although some of these fungi have been identified and characterized, we still know little about the microbial ecology of wild blueberry. Our study aims to characterize the fungal and bacterial rhizosphere communities of Vaccinium angustifolium (the main species encountered in wild blueberry fields). Our results clearly show that the fungal order Helotiales was the most abundant taxon associated with V. angustifolium. Helotiales contains most of the known ericoid mycorrhizal fungi which are expected to dominate in such a biotope. Furthermore, we found the dominant bacterial order was the nitrogen-fixing Rhizobiales. The Bradyrhizobium genus, whose members are known to form nodules with legumes, was among the 10 most abundant genera in the bacterial communities. In addition, Bradyrhizobium and Roseiarcus sequences significantly correlated with higher leaf-nitrogen content. Overall, our data documented fungal and bacterial community structure differences in three wild blueberry production fields.
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Into the wild blueberry (Vaccinium angustifolium) rhizosphere microbiota.
Environmental microbiology, 2020Co-Authors: Simon Morvan, Hacène Meglouli, Anissa Lounès-hadj Sahraoui, Mohamed HijriAbstract:The ability of wild blueberries to adapt to their harsh environment is believed to be closely related to their symbiosis with ericoid mycorrhizal fungi which produce enzymes capable of organic matter mineralization. Although some of these fungi have been identified and characterized, we still know little about the microbial ecology of wild blueberry. Our study aims to characterize the fungal and bacterial rhizosphere communities of Vaccinium angustifolium (the main species encountered in wild blueberry fields). Our results clearly show that the fungal order Helotiales was the most abundant taxon associated with V. angustifolium. Helotiales contains most of the known ericoid mycorrhizal fungi which are expected to dominate in such a biotope. Furthermore, we found the dominant bacterial order was the nitrogen-fixing Rhizobiales. The Bradyrhizobium genus whose members are known to form nodules with legumes, was among the ten most abundant genera in the bacterial communities. In addition, Bradyrhizobium and Roseiarcus sequences significantly correlated with higher leaf-nitrogen content. Overall our data documented fungal and bacterial community structure differences in three wild blueberry production fields. This article is protected by copyright. All rights reserved.
Andrée Gruslin - One of the best experts on this subject based on the ideXlab platform.
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Vaccinium angustifolium lowbush blueberry leaf extract increases extravillous trophoblast cell migration and invasion in vitro
Phytotherapy Research, 2018Co-Authors: Christina Ly, Jonathan Ferrier, Jeremiah Gaudet, John T. Arnason, Andrée Gruslin, Shannon A. Bainbridge, Julien YockelllelievreAbstract:: Perturbations to extravillous trophoblast (EVT) cell migration and invasion are associated with the development of placenta-mediated diseases. Phytochemicals found in the lowbush blueberry plant (Vaccinium angustifolium) have been shown to influence cell migration and invasion in models of tumorigenesis and noncancerous, healthy cells, however never in EVT cells. We hypothesized that the phenolic compounds present in V. angustifolium leaf extract promote trophoblast migration and invasion. Using the HTR-8/SVneo human EVT cell line and Boyden chamber assays, the influence of V. angustifolium leaf extract (0 to 2 × 104 ng/ml) on trophoblast cell migration (n = 4) and invasion (n = 4) was determined. Cellular proliferation and viability were assessed using immunoreactivity to Ki67 (n = 3) and trypan blue exclusion assays (n = 3), respectively. At 20 ng/ml, V. angustifolium leaf extract increased HTR-8/SVneo cell migration and invasion (p < .01) and did not affect cell proliferation or viability. Chlorogenic acid was identified as a major phenolic compound of the leaf extract and the most active compound. Evidence from Western blot analysis (n = 3) suggests that the effects of the leaf extract and chlorogenic acid on trophoblast migration and invasion are mediated through an adenosine monophosphate-activated protein (AMP) kinase-dependent mechanism. Further investigations examining the potential therapeutic applications of this natural health product extract and its major chemical compounds in the context of placenta-mediated diseases are warranted.
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Vaccinium angustifolium (lowbush blueberry) leaf extract increases extravillous trophoblast cell migration and invasion in vitro.
Phytotherapy research : PTR, 2018Co-Authors: Jonathan Ferrier, Jeremiah Gaudet, Julien Yockell-lelièvre, John T. Arnason, Andrée Gruslin, Shannon A. BainbridgeAbstract:Perturbations to extravillous trophoblast (EVT) cell migration and invasion are associated with the development of placenta-mediated diseases. Phytochemicals found in the lowbush blueberry plant (Vaccinium angustifolium) have been shown to influence cell migration and invasion in models of tumorigenesis and noncancerous, healthy cells, however never in EVT cells. We hypothesized that the phenolic compounds present in V. angustifolium leaf extract promote trophoblast migration and invasion. Using the HTR-8/SVneo human EVT cell line and Boyden chamber assays, the influence of V. angustifolium leaf extract (0 to 2 × 104 ng/ml) on trophoblast cell migration (n = 4) and invasion (n = 4) was determined. Cellular proliferation and viability were assessed using immunoreactivity to Ki67 (n = 3) and trypan blue exclusion assays (n = 3), respectively. At 20 ng/ml, V. angustifolium leaf extract increased HTR-8/SVneo cell migration and invasion (p
