The Experts below are selected from a list of 3252 Experts worldwide ranked by ideXlab platform
David Shveiky - One of the best experts on this subject based on the ideXlab platform.
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age associated impairments in tissue strength and immune response in a rat Vaginal Injury model
International Urogynecology Journal, 2020Co-Authors: David Shveiky, Cheryl B Iglesia, Srilekha Sarkar Das, Ofra Ben Menachemzidon, Henry H Chill, Kathryn SandbergAbstract:Surgical repair of pelvic organ prolapse often includes native tissue repair during which the patient’s own Vaginal connective tissue is used to achieve pelvic support. This method, based on plication and suspension often yields suboptimal anatomical outcomes, possibly due to inadequate healing of the Vaginal connective tissue. We hypothesized that age might have a negative effect on the time course and tissue biomechanics of Vaginal wound healing in a rat model. Fifty young (12 weeks) and old (12 months) female 344BN Fischer rats were subjected to a posterior midline Vaginal incision. The time course of repair was determined by measuring the size of the wound on days 1, 3, 7, and 14 post-Injury. These findings correlated with the immune response to Injury using a marker of impaired wound healing, the inflammatory cytokine macrophage migration inhibitory factor in the Vaginal muscularis. Biomechanical properties of the healed Vaginal tissue were tested 30 days post-Injury. Wound healing was assessed on days 1, 3, 7, and 14 post-Injury. On day 3 post-Injury, the wounds in the young animals had all closed whereas the wounds in the old animals remained open. Furthermore, on day 7, the wound gap was still filled with granulation tissue in the old rats, whereas for the young rats, the wound area was almost indistinguishable from the non-injured area. Macrophage migration inhibitory factor was highly expressed in the Vaginal epithelium and in the Vaginal muscularis after Injury. When compared with young animals, macrophage migration inhibitory factor levels of old rats began to rise more than 2 days later and the increased tissue expression persisted for 7 days longer. The breakpoint force of the healed vagina of old rats was almost 4-fold weaker than in young rats. At 30 days post-Injury, the healed vagina in old rats regained less of the original (healthy) force at breakpoint than the young rats. In this rat model, age impaired Vaginal wound healing, which was reflected in the altered inflammatory response to Injury and reduced tissue strength.
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systemically transplanted mesenchymal stem cells induce vascular like structure formation in a rat model of Vaginal Injury
PLOS ONE, 2019Co-Authors: Ben Menachemzidon O, Michal Gropp, Ben Shushan E, Benjamin Reubinoff, David ShveikyAbstract:The beneficial effect of mesenchymal stem cells (MSCs) on wound healing is mostly attributed to a trophic effect that promotes angiogenesis. Whether MSCs can contribute to the formation of new blood vessels by direct differentiation is still controversial. Pelvic floor dysfunction (PFD) is a group of disorders that negatively affect the quality of women’s lives. Traditional Vaginal surgical repair provides disappointing anatomical outcome. Stem cell transplantation may be used to supplement surgery and improve its outcome. Here we aimed to examine the engraftment, survival, differentiation and angiogenic effect of transplanted MSCs in a Vaginal Injury rat model. MSCs were obtained from the bone marrow of Sprague Drawley (SD) rats, expanded and characterized in vitro. The MSCs expressed CD90 and CD29, did not express CD45, CD34, CD11b and CD31 and could differentiate into osteogenic, chondrogenic and adipogenic lineages. Cells were labeled with either PKH-26 or GFP and transplanted systemically or locally to female SD rats, just after a standardized Vaginal incision was made. Engraftment after local transplantation was less efficient at all-time points compared to systemic administration. In the systemically transplanted animal group, MSCs migrated to the Injury site and were present in the healed vagina for at least 30 days. Both systemic and local MSCs transplantation promoted host angiogenesis. Systemically transplanted MSCs created new vascular-like structures by direct differentiation into endothelium. These findings pave the way to further studies of the potential role of MSCs transplantation in improving surgical outcome in women with PFD.
Gilleran Jason - One of the best experts on this subject based on the ideXlab platform.
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AMS-800 Artificial urinary sphincter in female patients with stress urinary incontinence: a systematic review
'Wiley', 2018Co-Authors: Peyronnet Benoit, O'connor Eabhann, Khavari Rose, Capon Grégoire, Manunta Andrea, Allue Marta, Hascoet Juliette, Nitti, Victor W., Gamé Xavier, Gilleran JasonAbstract:Aims: To perform a systematic review of studies reporting the outcomes of AMS-800 artificial urinary sphincter (AUS) implantation in female patients with stress urinary incontinence (SUI) resulting from intrinsic sphincter deficiency (ISD). Methods: A systematic literature search of the Medline and Embase databases was performed in June 2018 in accordance with the PRISMA statement. No time limit was used. The protocol was registered in PROSPERO (CRD42018099612). Study selection and data extraction were performed by two independent reviewers. Results: Of 886 records screened, 17 were included. All were retrospective or prospective non-comparative case series. One study reported on Vaginal AUS implantation, 11 on open AUS implantation, two on laparoscopic AUS implantation, two on robot-assisted AUS implantation and one compared open and robot-assisted implantations. The vast majority of patients had undergone at least one anti-incontinence surgical procedure prior to AUS implantation (69.1-100%). The intraoperative bladder neck Injury rates ranged from 0% to 43.8% and the intraoperative Vaginal Injury rates ranged from 0 to 25%. After mean follow-up periods ranging from 5 to 204 months, the complete continence rates ranged from 61.1% to 100%. The rates of explantation, erosion and mechanical failure varied from 0% to 45.3%, 0% to 22.2% and 0% to 44.1%, respectively. Conclusions: AMS-800 AUS can provide excellent functional outcomes in female patients with SUI resulting from ISD but at the cost of a relatively high morbidity. High level of evidence studies are needed to help better define the role of AUS in the female SUI armamentarium
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AMS-800 Artificial urinary sphincter in female patients with stress urinary incontinence: A systematic review.
'Wiley', 2018Co-Authors: Peyronnet Benoit, O'connor Eabhann, Khavari Rose, Capon Grégoire, Manunta Andrea, Allue Marta, Hascoet Juliette, Nitti, Victor W., Gamé Xavier, Gilleran JasonAbstract:peer reviewedAIMS: To perform a systematic review of studies reporting the outcomes of AMS-800 artificial urinary sphincter (AUS) implantation in female patients with stress urinary incontinence (SUI) resulting from intrinsic sphincter deficiency (ISD). METHODS: A systematic literature search of the Medline and Embase databases was performed in June 2018 in accordance with the PRISMA statement. No time limit was used. The protocol was registered in PROSPERO (CRD42018099612). Study selection and data extraction were performed by two independent reviewers. RESULTS: Of 886 records screened, 17 were included. All were retrospective or prospective non-comparative case series. One study reported on Vaginal AUS implantation, 11 on open AUS implantation, two on laparoscopic AUS implantation, two on robot-assisted AUS implantation and one compared open and robot-assisted implantations. The vast majority of patients had undergone at least one anti-incontinence surgical procedure prior to AUS implantation (69.1-100%). The intraoperative bladder neck Injury rates ranged from 0% to 43.8% and the intraoperative Vaginal Injury rates ranged from 0 to 25%. After mean follow-up periods ranging from 5 to 204 months, the complete continence rates ranged from 61.1% to 100%. The rates of explantation, erosion and mechanical failure varied from 0% to 45.3%, 0% to 22.2% and 0% to 44.1%, respectively. CONCLUSIONS: AMS-800 AUS can provide excellent functional outcomes in female patients with SUI resulting from ISD but at the cost of a relatively high morbidity. High level of evidence studies are needed to help better define the role of AUS in the female SUI armamentarium
Peyronnet Benoit - One of the best experts on this subject based on the ideXlab platform.
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AMS-800 Artificial urinary sphincter in female patients with stress urinary incontinence: a systematic review
'Wiley', 2018Co-Authors: Peyronnet Benoit, O'connor Eabhann, Khavari Rose, Capon Grégoire, Manunta Andrea, Allue Marta, Hascoet Juliette, Nitti, Victor W., Gamé Xavier, Gilleran JasonAbstract:Aims: To perform a systematic review of studies reporting the outcomes of AMS-800 artificial urinary sphincter (AUS) implantation in female patients with stress urinary incontinence (SUI) resulting from intrinsic sphincter deficiency (ISD). Methods: A systematic literature search of the Medline and Embase databases was performed in June 2018 in accordance with the PRISMA statement. No time limit was used. The protocol was registered in PROSPERO (CRD42018099612). Study selection and data extraction were performed by two independent reviewers. Results: Of 886 records screened, 17 were included. All were retrospective or prospective non-comparative case series. One study reported on Vaginal AUS implantation, 11 on open AUS implantation, two on laparoscopic AUS implantation, two on robot-assisted AUS implantation and one compared open and robot-assisted implantations. The vast majority of patients had undergone at least one anti-incontinence surgical procedure prior to AUS implantation (69.1-100%). The intraoperative bladder neck Injury rates ranged from 0% to 43.8% and the intraoperative Vaginal Injury rates ranged from 0 to 25%. After mean follow-up periods ranging from 5 to 204 months, the complete continence rates ranged from 61.1% to 100%. The rates of explantation, erosion and mechanical failure varied from 0% to 45.3%, 0% to 22.2% and 0% to 44.1%, respectively. Conclusions: AMS-800 AUS can provide excellent functional outcomes in female patients with SUI resulting from ISD but at the cost of a relatively high morbidity. High level of evidence studies are needed to help better define the role of AUS in the female SUI armamentarium
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AMS-800 Artificial urinary sphincter in female patients with stress urinary incontinence: A systematic review.
'Wiley', 2018Co-Authors: Peyronnet Benoit, O'connor Eabhann, Khavari Rose, Capon Grégoire, Manunta Andrea, Allue Marta, Hascoet Juliette, Nitti, Victor W., Gamé Xavier, Gilleran JasonAbstract:peer reviewedAIMS: To perform a systematic review of studies reporting the outcomes of AMS-800 artificial urinary sphincter (AUS) implantation in female patients with stress urinary incontinence (SUI) resulting from intrinsic sphincter deficiency (ISD). METHODS: A systematic literature search of the Medline and Embase databases was performed in June 2018 in accordance with the PRISMA statement. No time limit was used. The protocol was registered in PROSPERO (CRD42018099612). Study selection and data extraction were performed by two independent reviewers. RESULTS: Of 886 records screened, 17 were included. All were retrospective or prospective non-comparative case series. One study reported on Vaginal AUS implantation, 11 on open AUS implantation, two on laparoscopic AUS implantation, two on robot-assisted AUS implantation and one compared open and robot-assisted implantations. The vast majority of patients had undergone at least one anti-incontinence surgical procedure prior to AUS implantation (69.1-100%). The intraoperative bladder neck Injury rates ranged from 0% to 43.8% and the intraoperative Vaginal Injury rates ranged from 0 to 25%. After mean follow-up periods ranging from 5 to 204 months, the complete continence rates ranged from 61.1% to 100%. The rates of explantation, erosion and mechanical failure varied from 0% to 45.3%, 0% to 22.2% and 0% to 44.1%, respectively. CONCLUSIONS: AMS-800 AUS can provide excellent functional outcomes in female patients with SUI resulting from ISD but at the cost of a relatively high morbidity. High level of evidence studies are needed to help better define the role of AUS in the female SUI armamentarium
Ben Menachemzidon O - One of the best experts on this subject based on the ideXlab platform.
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systemically transplanted mesenchymal stem cells induce vascular like structure formation in a rat model of Vaginal Injury
PLOS ONE, 2019Co-Authors: Ben Menachemzidon O, Michal Gropp, Ben Shushan E, Benjamin Reubinoff, David ShveikyAbstract:The beneficial effect of mesenchymal stem cells (MSCs) on wound healing is mostly attributed to a trophic effect that promotes angiogenesis. Whether MSCs can contribute to the formation of new blood vessels by direct differentiation is still controversial. Pelvic floor dysfunction (PFD) is a group of disorders that negatively affect the quality of women’s lives. Traditional Vaginal surgical repair provides disappointing anatomical outcome. Stem cell transplantation may be used to supplement surgery and improve its outcome. Here we aimed to examine the engraftment, survival, differentiation and angiogenic effect of transplanted MSCs in a Vaginal Injury rat model. MSCs were obtained from the bone marrow of Sprague Drawley (SD) rats, expanded and characterized in vitro. The MSCs expressed CD90 and CD29, did not express CD45, CD34, CD11b and CD31 and could differentiate into osteogenic, chondrogenic and adipogenic lineages. Cells were labeled with either PKH-26 or GFP and transplanted systemically or locally to female SD rats, just after a standardized Vaginal incision was made. Engraftment after local transplantation was less efficient at all-time points compared to systemic administration. In the systemically transplanted animal group, MSCs migrated to the Injury site and were present in the healed vagina for at least 30 days. Both systemic and local MSCs transplantation promoted host angiogenesis. Systemically transplanted MSCs created new vascular-like structures by direct differentiation into endothelium. These findings pave the way to further studies of the potential role of MSCs transplantation in improving surgical outcome in women with PFD.
Reuben M Peyser - One of the best experts on this subject based on the ideXlab platform.
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Vaginal Injury resulting from sliding down a water chute
American Journal of Obstetrics and Gynecology, 1992Co-Authors: Jacob Niv, Joseph B Lessing, Joseph Hartuv, Reuben M PeyserAbstract:A 27-year-old woman was admitted to the hospital because of profuse Vaginal bleeding from a Vaginal laceration caused by the impact of water to the perineum after sliding down a water chute. At the time of the accident the patient was using a Vaginal tampon. The potential mechanism for this trauma is discussed.
