The Experts below are selected from a list of 327 Experts worldwide ranked by ideXlab platform
Peter J.f. Snijders - One of the best experts on this subject based on the ideXlab platform.
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methylation marker analysis of self sampled cervico Vaginal Lavage specimens to triage high risk hpv positive women for colposcopy
International Journal of Cancer, 2014Co-Authors: Albertus T. Hesselink, Daniëlle A.m. Heideman, Renske D.m. Steenbergen, Murat Gök, F. J. Van Kemenade, Saskia M. Wilting, Johannes Berkhof, C.j.l.m. Meijer, Peter J.f. SnijdersAbstract:Methylation markers were studied for their suitability to triage human papillomavirus (HPV)-positive women by testing self-collected cervico-Vaginal Lavage specimens. For this purpose, we analyzed 355 hrHPV-positive self-collected specimens with three methylation markers, that is, CADM1-m18, MAL-m1 and miR-124-2 by quantitative methylation-specific PCR. The areas under the receiver-operating characteristic (ROC) curve for end-point cervical intraepithelial neoplasia grade 3 or worse (CIN3+) were 0.637 for CADM1-m18, 0.767 for MAL-m1 and 0.762 for miR-124-2. This indicates that CADM1-m18 is not suitable as single marker. By varying the thresholds of both markers in the bi-marker panels CADM1-m18/MAL-m1, CADM1-m18/miR-124-2 and MAL-m1/miR-124-2 upper and lower ROC curves were obtained, depicting the maximum and minimum CIN3+ sensitivity, respectively, at given specificity. For all these bi-marker combinations, the upper curves were similar. However, for the MAL-m1/miR-124-2 panel, the distance between upper and lower ROC curves was closest and this panel displayed the highest assay thresholds, indicating that this combination was most robust. At clinical specificities of 50 and 70%, the MAL-m1/miR-124-2 sensitivity for detection of CIN3+ ranged from 77.0 to 87.8% and from 64.9 to 71.6%, respectively. At 70% specificity thresholds no carcinomas were missed. By comparison, the CIN3+ sensitivity of HPV16/18 genotyping on the self-sampled Lavage specimens was 58.1% (95%CI: 46.6-68.8) at a specificity of 87.7% (95%CI: 83.2-91.2). In conclusion, methylation analysis is a promising triage tool that in combination with HPV-DNA testing offers feasible, full molecular screening on self-collected cervico-Vaginal Lavage specimens.
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Methylation marker analysis of self‐sampled cervico‐Vaginal Lavage specimens to triage high‐risk HPV‐positive women for colposcopy
International journal of cancer, 2014Co-Authors: Albertus T. Hesselink, Daniëlle A.m. Heideman, Renske D.m. Steenbergen, Murat Gök, F. J. Van Kemenade, Saskia M. Wilting, Johannes Berkhof, C.j.l.m. Meijer, Peter J.f. SnijdersAbstract:Methylation markers were studied for their suitability to triage human papillomavirus (HPV)-positive women by testing self-collected cervico-Vaginal Lavage specimens. For this purpose, we analyzed 355 hrHPV-positive self-collected specimens with three methylation markers, that is, CADM1-m18, MAL-m1 and miR-124-2 by quantitative methylation-specific PCR. The areas under the receiver-operating characteristic (ROC) curve for end-point cervical intraepithelial neoplasia grade 3 or worse (CIN3+) were 0.637 for CADM1-m18, 0.767 for MAL-m1 and 0.762 for miR-124-2. This indicates that CADM1-m18 is not suitable as single marker. By varying the thresholds of both markers in the bi-marker panels CADM1-m18/MAL-m1, CADM1-m18/miR-124-2 and MAL-m1/miR-124-2 upper and lower ROC curves were obtained, depicting the maximum and minimum CIN3+ sensitivity, respectively, at given specificity. For all these bi-marker combinations, the upper curves were similar. However, for the MAL-m1/miR-124-2 panel, the distance between upper and lower ROC curves was closest and this panel displayed the highest assay thresholds, indicating that this combination was most robust. At clinical specificities of 50 and 70%, the MAL-m1/miR-124-2 sensitivity for detection of CIN3+ ranged from 77.0 to 87.8% and from 64.9 to 71.6%, respectively. At 70% specificity thresholds no carcinomas were missed. By comparison, the CIN3+ sensitivity of HPV16/18 genotyping on the self-sampled Lavage specimens was 58.1% (95%CI: 46.6-68.8) at a specificity of 87.7% (95%CI: 83.2-91.2). In conclusion, methylation analysis is a promising triage tool that in combination with HPV-DNA testing offers feasible, full molecular screening on self-collected cervico-Vaginal Lavage specimens.
Albertus T. Hesselink - One of the best experts on this subject based on the ideXlab platform.
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1Department of Pathology, VU University Medical Center, (VUmc) DeConclusions: Replacing the first generation self-samplingHPV DNA results
2016Co-Authors: Viola Mj Verhoef, Albertus T. Hesselink, Johannes Berkhof, Maaike G Dijkstra, Remko P Bosgraaf, Willem Jg Melchers, Ruud Lm Bekkers, Folkert J Van KemenadeAbstract:A second generation cervico-Vaginal Lavage device shows similar performance as its spect to DNA yield and device resulted in equal DNA yields, comparable hrHPV positivity rates and similar response rates. Therefore, it can Verhoef et al. BMC Women's Health 2013, 13:2
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methylation marker analysis of self sampled cervico Vaginal Lavage specimens to triage high risk hpv positive women for colposcopy
International Journal of Cancer, 2014Co-Authors: Albertus T. Hesselink, Daniëlle A.m. Heideman, Renske D.m. Steenbergen, Murat Gök, F. J. Van Kemenade, Saskia M. Wilting, Johannes Berkhof, C.j.l.m. Meijer, Peter J.f. SnijdersAbstract:Methylation markers were studied for their suitability to triage human papillomavirus (HPV)-positive women by testing self-collected cervico-Vaginal Lavage specimens. For this purpose, we analyzed 355 hrHPV-positive self-collected specimens with three methylation markers, that is, CADM1-m18, MAL-m1 and miR-124-2 by quantitative methylation-specific PCR. The areas under the receiver-operating characteristic (ROC) curve for end-point cervical intraepithelial neoplasia grade 3 or worse (CIN3+) were 0.637 for CADM1-m18, 0.767 for MAL-m1 and 0.762 for miR-124-2. This indicates that CADM1-m18 is not suitable as single marker. By varying the thresholds of both markers in the bi-marker panels CADM1-m18/MAL-m1, CADM1-m18/miR-124-2 and MAL-m1/miR-124-2 upper and lower ROC curves were obtained, depicting the maximum and minimum CIN3+ sensitivity, respectively, at given specificity. For all these bi-marker combinations, the upper curves were similar. However, for the MAL-m1/miR-124-2 panel, the distance between upper and lower ROC curves was closest and this panel displayed the highest assay thresholds, indicating that this combination was most robust. At clinical specificities of 50 and 70%, the MAL-m1/miR-124-2 sensitivity for detection of CIN3+ ranged from 77.0 to 87.8% and from 64.9 to 71.6%, respectively. At 70% specificity thresholds no carcinomas were missed. By comparison, the CIN3+ sensitivity of HPV16/18 genotyping on the self-sampled Lavage specimens was 58.1% (95%CI: 46.6-68.8) at a specificity of 87.7% (95%CI: 83.2-91.2). In conclusion, methylation analysis is a promising triage tool that in combination with HPV-DNA testing offers feasible, full molecular screening on self-collected cervico-Vaginal Lavage specimens.
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Methylation marker analysis of self‐sampled cervico‐Vaginal Lavage specimens to triage high‐risk HPV‐positive women for colposcopy
International journal of cancer, 2014Co-Authors: Albertus T. Hesselink, Daniëlle A.m. Heideman, Renske D.m. Steenbergen, Murat Gök, F. J. Van Kemenade, Saskia M. Wilting, Johannes Berkhof, C.j.l.m. Meijer, Peter J.f. SnijdersAbstract:Methylation markers were studied for their suitability to triage human papillomavirus (HPV)-positive women by testing self-collected cervico-Vaginal Lavage specimens. For this purpose, we analyzed 355 hrHPV-positive self-collected specimens with three methylation markers, that is, CADM1-m18, MAL-m1 and miR-124-2 by quantitative methylation-specific PCR. The areas under the receiver-operating characteristic (ROC) curve for end-point cervical intraepithelial neoplasia grade 3 or worse (CIN3+) were 0.637 for CADM1-m18, 0.767 for MAL-m1 and 0.762 for miR-124-2. This indicates that CADM1-m18 is not suitable as single marker. By varying the thresholds of both markers in the bi-marker panels CADM1-m18/MAL-m1, CADM1-m18/miR-124-2 and MAL-m1/miR-124-2 upper and lower ROC curves were obtained, depicting the maximum and minimum CIN3+ sensitivity, respectively, at given specificity. For all these bi-marker combinations, the upper curves were similar. However, for the MAL-m1/miR-124-2 panel, the distance between upper and lower ROC curves was closest and this panel displayed the highest assay thresholds, indicating that this combination was most robust. At clinical specificities of 50 and 70%, the MAL-m1/miR-124-2 sensitivity for detection of CIN3+ ranged from 77.0 to 87.8% and from 64.9 to 71.6%, respectively. At 70% specificity thresholds no carcinomas were missed. By comparison, the CIN3+ sensitivity of HPV16/18 genotyping on the self-sampled Lavage specimens was 58.1% (95%CI: 46.6-68.8) at a specificity of 87.7% (95%CI: 83.2-91.2). In conclusion, methylation analysis is a promising triage tool that in combination with HPV-DNA testing offers feasible, full molecular screening on self-collected cervico-Vaginal Lavage specimens.
Mimi Ghosh - One of the best experts on this subject based on the ideXlab platform.
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Reduced levels of genital tract immune biomarkers in postmenopausal women: implications for HIV acquisition.
American journal of obstetrics and gynecology, 2016Co-Authors: Mariel Jais, Naji Younes, Stacey Chapman, Susan Cu-uvin, Mimi GhoshAbstract:Rates of HIV infections are increasing in older adults. Although it is known that the HIV/AIDS epidemics affects women disproportionately, little is known regarding immune functions in the genital tract of postmenopausal women, as relevant to HIV susceptibility. The objective of the study was to compare levels of female reproductive tract immune mediators that are important for HIV-associated immune responses as well as intrinsic anti-HIV activity in the cervical Vaginal Lavages collected from HIV-negative pre- and postmenopausal women. Cervical Vaginal Lavage from 20 premenopausal and 20 postmenopausal women were assayed for interleukin-6, interleukin-8, tumor necrosis factor-α, secretory leukocyte protease inhibitor, elafin, human β-defensin-2, and macrophage inflammatory protein-3α using standard enzyme-linked immunosorbent assays. Anti-HIV activity of cervical-Vaginal Lavage was measured using TZM-bl indicator cells against HIV-1 IIIB and BaL. Whereas each postmenopausal woman provided only 1 sample, each premenopausal woman provided 3 samples, during proliferative, ovulatory, and secretory stages, based on menstrual dates. We observed significantly lower levels of tumor necrosis factor-α, MIP-3α, secretory leukocyte protease inhibitor, elafin, and human β-defensin-2 in cervical Vaginal Lavage from postmenopausal women compared with premenopausal women. Inhibition of HIV-1 infection was observed for both pre- and postmenopausal women, but cervical Vaginal Lavage from postmenopausal women showed significantly higher inhibition against HIV-1 BaL after adjusting for total protein concentration, genital pH, and reproductive tract infections. No change in mediators or HIV inhibition was observed through the stages of menstrual cycle. In addition, we observed that postmenopausal women with reproductive tract infections had significantly higher levels of tumor necrosis factor-α and significantly lower levels of interleukin-8, which were not observed in premenopausal women. Our findings suggest that female reproductive tract immune microenvironment is distinct in HIV-negative postmenopausal women. Further studies are needed to assess the risk of HIV acquisition/transmission in this population. Copyright © 2016 Elsevier Inc. All rights reserved.
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Reduced levels of genital tract immune biomarkers in postmenopausal women: implications for HIV acquisition
American Journal of Obstetrics and Gynecology, 2016Co-Authors: Mariel Jais, Naji Younes, Stacey Chapman, Susan Cu-uvin, Mimi GhoshAbstract:Background Rates of HIV infections are increasing in older adults. Although it is known that the HIV/AIDS epidemics affects women disproportionately, little is known regarding immune functions in the genital tract of postmenopausal women, as relevant to HIV susceptibility. Objective The objective of the study was to compare levels of female reproductive tract immune mediators that are important for HIV-associated immune responses as well as intrinsic anti-HIV activity in the cervical Vaginal Lavages collected from HIV-negative pre- and postmenopausal women. Study Design Cervical Vaginal Lavage from 20 premenopausal and 20 postmenopausal women were assayed for interleukin-6, interleukin-8, tumor necrosis factor-α, secretory leukocyte protease inhibitor, elafin, human β-defensin-2, and macrophage inflammatory protein-3α using standard enzyme-linked immunosorbent assays. Anti-HIV activity of cervical-Vaginal Lavage was measured using TZM-bl indicator cells against HIV-1 IIIB and BaL. Whereas each postmenopausal woman provided only 1 sample, each premenopausal woman provided 3 samples, during proliferative, ovulatory, and secretory stages, based on menstrual dates. Results We observed significantly lower levels of tumor necrosis factor-α, MIP-3α, secretory leukocyte protease inhibitor, elafin, and human β-defensin-2 in cervical Vaginal Lavage from postmenopausal women compared with premenopausal women. Inhibition of HIV-1 infection was observed for both pre- and postmenopausal women, but cervical Vaginal Lavage from postmenopausal women showed significantly higher inhibition against HIV-1 BaL after adjusting for total protein concentration, genital pH, and reproductive tract infections. No change in mediators or HIV inhibition was observed through the stages of menstrual cycle. In addition, we observed that postmenopausal women with reproductive tract infections had significantly higher levels of tumor necrosis factor-α and significantly lower levels of interleukin-8, which were not observed in premenopausal women. Conclusion Our findings suggest that female reproductive tract immune microenvironment is distinct in HIV-negative postmenopausal women. Further studies are needed to assess the risk of HIV acquisition/transmission in this population.
Patricia D. Ndhlovu - One of the best experts on this subject based on the ideXlab platform.
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The effects of genital schistosoma haematobium on human papillomavirus and the development of cervical neoplasia after five years in a Zimbabwean population.
European journal of gynaecological oncology, 2010Co-Authors: Eyrun Floerecke Kjetland, E. Gomo, Nicholas Midzi, Lovemore Gwanzura, Patricia D. Ndhlovu, Takafira Mduluza, Vanessa Deschoolmeester, Peter R. Mason, Jan B. Vermorken, Henrik FriisAbstract:Background High-risk human papillomavirus (HPV) is responsible for cervical cancer and genital Schistosoma haematobium infection has been hypothesized to be an additional co-factor or even an independent risk factor for cervical neoplasia. The present study aimed to investigate the impact of schistosomiasis on HPV persistence and development of cell atypia in a group of rural Zimbabwean women with confirmed high-risk HPV. Methods A five-year follow-up was done among women previously included in a study on genital schistosomiasis. Women who had high-risk HPV at baseline were invited after 5 years for examination of cell atypia, genital schistosomiasis, and high-risk HPV. Both Vaginal Lavage samples (low-cost) and cervix brush samples (high-cost) were obtained for further analysis. Results Thirty-seven women were re-examined. Genital Schistosoma haematobium of a minimum of five years' duration was associated with the development high-grade squamous intraepithelial neoplasia, but not with persistent high-risk HPV. There was a high concordance between the brush and Vaginal Lavage (96.3% agreement, kappa 0.93); however, the number of beta-globin negative Vaginal Lavage samples was unacceptably high. Conclusions Findings warrant an exploration in a larger longitudinal study where a Vaginal swab should be explored.
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Schistosomiasis PCR in Vaginal Lavage as an indicator of genital Schistosoma haematobium infection in rural Zimbabwean women.
The American journal of tropical medicine and hygiene, 2009Co-Authors: Eyrun Floerecke Kjetland, Svein Gunnar Gundersen, Robert J. Ten Hove, E. Gomo, Nicholas Midzi, Lovemore Gwanzura, Peter W. Mason, Henrik Friis, Jaco J. Verweij, Patricia D. NdhlovuAbstract:Schistosoma real-time polymerase chain reaction (PCR) is sensitive and specific in urine and stool. We sought to explore the relationship between genital schistosomiasis and the Schistosoma PCR in women. PCR was run on 83 Vaginal Lavage samples from a rural Zimbabwean population. Women underwent clinical and colposcopic investigations, analyses for sexually transmitted infections, and genital schistosomiasis. Thirty samples were positive for Schistosoma PCR: 12 were strong and 18 were weak positive. Sensitivity (67%) and specificity (83%) were best in women below the age of 25 years. A positive schistosome PCR result was associated with S. haematobium ova in genital tissue, so-called sandy patches, and bleeding. Prevalence determined by PCR were lower and real-time PCR values were weaker in older women. The presence of Schistosoma DNA may be greater in the recent lesions (e.g., in younger women). For diagnosis in rural areas and in large studies, Schistosoma PCR could become a supplement to gynecologic examinations.
Susan Cu-uvin - One of the best experts on this subject based on the ideXlab platform.
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Reduced levels of genital tract immune biomarkers in postmenopausal women: implications for HIV acquisition.
American journal of obstetrics and gynecology, 2016Co-Authors: Mariel Jais, Naji Younes, Stacey Chapman, Susan Cu-uvin, Mimi GhoshAbstract:Rates of HIV infections are increasing in older adults. Although it is known that the HIV/AIDS epidemics affects women disproportionately, little is known regarding immune functions in the genital tract of postmenopausal women, as relevant to HIV susceptibility. The objective of the study was to compare levels of female reproductive tract immune mediators that are important for HIV-associated immune responses as well as intrinsic anti-HIV activity in the cervical Vaginal Lavages collected from HIV-negative pre- and postmenopausal women. Cervical Vaginal Lavage from 20 premenopausal and 20 postmenopausal women were assayed for interleukin-6, interleukin-8, tumor necrosis factor-α, secretory leukocyte protease inhibitor, elafin, human β-defensin-2, and macrophage inflammatory protein-3α using standard enzyme-linked immunosorbent assays. Anti-HIV activity of cervical-Vaginal Lavage was measured using TZM-bl indicator cells against HIV-1 IIIB and BaL. Whereas each postmenopausal woman provided only 1 sample, each premenopausal woman provided 3 samples, during proliferative, ovulatory, and secretory stages, based on menstrual dates. We observed significantly lower levels of tumor necrosis factor-α, MIP-3α, secretory leukocyte protease inhibitor, elafin, and human β-defensin-2 in cervical Vaginal Lavage from postmenopausal women compared with premenopausal women. Inhibition of HIV-1 infection was observed for both pre- and postmenopausal women, but cervical Vaginal Lavage from postmenopausal women showed significantly higher inhibition against HIV-1 BaL after adjusting for total protein concentration, genital pH, and reproductive tract infections. No change in mediators or HIV inhibition was observed through the stages of menstrual cycle. In addition, we observed that postmenopausal women with reproductive tract infections had significantly higher levels of tumor necrosis factor-α and significantly lower levels of interleukin-8, which were not observed in premenopausal women. Our findings suggest that female reproductive tract immune microenvironment is distinct in HIV-negative postmenopausal women. Further studies are needed to assess the risk of HIV acquisition/transmission in this population. Copyright © 2016 Elsevier Inc. All rights reserved.
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Reduced levels of genital tract immune biomarkers in postmenopausal women: implications for HIV acquisition
American Journal of Obstetrics and Gynecology, 2016Co-Authors: Mariel Jais, Naji Younes, Stacey Chapman, Susan Cu-uvin, Mimi GhoshAbstract:Background Rates of HIV infections are increasing in older adults. Although it is known that the HIV/AIDS epidemics affects women disproportionately, little is known regarding immune functions in the genital tract of postmenopausal women, as relevant to HIV susceptibility. Objective The objective of the study was to compare levels of female reproductive tract immune mediators that are important for HIV-associated immune responses as well as intrinsic anti-HIV activity in the cervical Vaginal Lavages collected from HIV-negative pre- and postmenopausal women. Study Design Cervical Vaginal Lavage from 20 premenopausal and 20 postmenopausal women were assayed for interleukin-6, interleukin-8, tumor necrosis factor-α, secretory leukocyte protease inhibitor, elafin, human β-defensin-2, and macrophage inflammatory protein-3α using standard enzyme-linked immunosorbent assays. Anti-HIV activity of cervical-Vaginal Lavage was measured using TZM-bl indicator cells against HIV-1 IIIB and BaL. Whereas each postmenopausal woman provided only 1 sample, each premenopausal woman provided 3 samples, during proliferative, ovulatory, and secretory stages, based on menstrual dates. Results We observed significantly lower levels of tumor necrosis factor-α, MIP-3α, secretory leukocyte protease inhibitor, elafin, and human β-defensin-2 in cervical Vaginal Lavage from postmenopausal women compared with premenopausal women. Inhibition of HIV-1 infection was observed for both pre- and postmenopausal women, but cervical Vaginal Lavage from postmenopausal women showed significantly higher inhibition against HIV-1 BaL after adjusting for total protein concentration, genital pH, and reproductive tract infections. No change in mediators or HIV inhibition was observed through the stages of menstrual cycle. In addition, we observed that postmenopausal women with reproductive tract infections had significantly higher levels of tumor necrosis factor-α and significantly lower levels of interleukin-8, which were not observed in premenopausal women. Conclusion Our findings suggest that female reproductive tract immune microenvironment is distinct in HIV-negative postmenopausal women. Further studies are needed to assess the risk of HIV acquisition/transmission in this population.
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The Menstrual Cycle Does Not Affect Human Immunodeficiency Virus Type 1 Levels in Vaginal Secretions
The Journal of infectious diseases, 2002Co-Authors: Patricia Reichelderfer, Alan L. Landay, Susan Cu-uvin, Andrea Kovacs, David J. Wright, David N. Burns, Jonathan A. Cohn, Robert W. CoombsAbstract:To determine whether the menstrual cycle affects human immunodeficiency virus (HIV) type 1 levels in Vaginal secretions, Vaginal Lavage samples were collected at 7, 14, and 21 days after initiation of menses, to compare virus levels during the follicular, ovulatory, and luteal phases. During 33 menstrual cycles in 25 women, HIV-1 RNA levels in Vaginal secretions ranged from .05). These results suggest that hormonal changes during the menstrual cycle do not have a significant effect on HIV-1 RNA levels in Vaginal secretions.
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Comparative analysis of methods for collection and measurement of immunoglobulins in cervical and Vaginal secretions of women.
Journal of immunological methods, 1997Co-Authors: Anne Quesnel, Susan Cu-uvin, Deirdre Murphy, Rhoda Ashley, Timothy P. Flanigan, Marian R. NeutraAbstract:The purpose of this study was to systematically compare 3 collection methods, Sno-strips, wicks and cervical-Vaginal Lavage, for analysis of immunoglobulin concentrations in female genital secretions. In each of 8 women, absorbent wicks and Sno-strips were applied at 4 locations: the lateral wall of the vagina; the posterior Vaginal fornix; the surface of the exocervix; and the endocervical canal. Cervical-Vaginal Lavage was then performed in 4 women with 5 ml PBS. Immunoglobulin and protein concentrations in Lavage samples were generally over 100 times lower than in the secretions captured directly from mucosal surfaces with either Sno-strips or wicks. Capture of undiluted secretions with either wicks or Sno-strips allowed calculation of actual immunoglobulin concentrations at specific mucosal sites: for example, median IgA levels were consistently highest in the endocervix and lowest in the vagina. Such information may be crucial in evaluating the correlates of protective immunity against micro-organisms that infect or invade discrete regions of the genital mucosa.
