The Experts below are selected from a list of 273 Experts worldwide ranked by ideXlab platform
Andrea Buettner - One of the best experts on this subject based on the ideXlab platform.
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sperm activating odorous substances in human follicular fluid and Vaginal Secretion identification by gas chromatography olfactometry and ca2 imaging
ChemPlusChem, 2013Co-Authors: Constanze Hartmann, Annika Triller, Marc Spehr, Ralf Dittrich, Hanns Hatt, Andrea BuettnerAbstract:Human sperm chemotaxis to follicular fluid has been well established, but the molecular mechanism(s) for this phenomenon are still largely unclear. Studies indicate that odorant receptors expressed on spermatozoa could play a role in this scenario. It has recently been shown that several synthetic floral scents activate the receptors OR1D2, OR4D1, and OR7A5 in vitro and evoke distinct sperm motility patterns in vivo. However, all agonists found so far are of synthetic origin and, thus, the presence of endogenous structural analogues in female bodily fluids is subject to speculation. Therefore, the aim of the study reported herein was to investigate the occurrence of odor compounds in Vaginal Secretions and follicular fluid by using gas chromatography-olfactometry. Chemically identified constituents of either bodily fluid were then analyzed for activation of recombinant chemoreceptors and candidate ligands were further tested for induction of sperm Ca2+ signals. Through using this approach, two novel odorant receptor-ligand pairs are reported and human sperm Ca2+ elevations in response to both odorous substances, namely 5α-androst-16-en-3-one and 4-hydroxy-2,5-dimethyl-3(2H)-furanone, are shown.
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Sperm‐Activating Odorous Substances in Human Follicular Fluid and Vaginal Secretion: Identification by Gas Chromatography–Olfactometry and Ca2+ Imaging
ChemPlusChem, 2013Co-Authors: Constanze Hartmann, Annika Triller, Marc Spehr, Ralf Dittrich, Hanns Hatt, Andrea BuettnerAbstract:Human sperm chemotaxis to follicular fluid has been well established, but the molecular mechanism(s) for this phenomenon are still largely unclear. Studies indicate that odorant receptors expressed on spermatozoa could play a role in this scenario. It has recently been shown that several synthetic floral scents activate the receptors OR1D2, OR4D1, and OR7A5 in vitro and evoke distinct sperm motility patterns in vivo. However, all agonists found so far are of synthetic origin and, thus, the presence of endogenous structural analogues in female bodily fluids is subject to speculation. Therefore, the aim of the study reported herein was to investigate the occurrence of odor compounds in Vaginal Secretions and follicular fluid by using gas chromatography-olfactometry. Chemically identified constituents of either bodily fluid were then analyzed for activation of recombinant chemoreceptors and candidate ligands were further tested for induction of sperm Ca2+ signals. Through using this approach, two novel odorant receptor-ligand pairs are reported and human sperm Ca2+ elevations in response to both odorous substances, namely 5α-androst-16-en-3-one and 4-hydroxy-2,5-dimethyl-3(2H)-furanone, are shown.
Urban Forsum - One of the best experts on this subject based on the ideXlab platform.
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trimethylamine and trimethylamine oxide levels in normal women and women with bacterial vaginosis reflect a local metabolism in Vaginal Secretion as compared to urine
Apmis, 2005Co-Authors: Helen Wolrath, Anders Hallen, Bengt Stahlbom, Urban ForsumAbstract:The smell of rotten fish is one of the characteristics of bacterial vaginosis (BV), and is due to trimethylamine (TMA). Trimethylamine can be found in human urine, although most of it occurs as the nonvolatile oxide (TMAO) form. The fraction TMA/TMAO can be expected to be the same in different body fluids if no local production of TMA occurs. In women with BV, TMAO in the Vaginal fluid is expected to be chemically reduced by the local bacterial flora to the much more odorous TMA. We have therefore studied the local Vaginal production of TMA in Vaginal Secretion compared to the general TMA-TMAO metabolism that was measured in urine using gas chromatography. Both Vaginal fluid and random urine samples were collected from women, with and without BV, attending a Swedish clinic for sexually transmitted diseases, and these samples were analyzed for TMA and TMAO. The results show that a local production of TMA occurs in the vagina that is not part of the general metabolism of TMA-TMAO.
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trimethylamine content in Vaginal Secretion and its relation to bacterial vaginosis
Apmis, 2002Co-Authors: Helen Wolrath, Hans Boren, Anders Hallen, Urban ForsumAbstract:The presence of a fishy odor emanating from women who present with a malodorous Vaginal discharge is well known. The odor is due to bacterial reduction of trimethylamine oxide to trimethylamine (TMA) in Vaginal Secretion. The release of TMA from specimens of Vaginal fluid following the addition of alkali is often used in making a clinical diagnosis of bacterial vaginosis (BV). We now report a sensitive gas chromatographic method for analysis and quantification of TMA in Vaginal fluid in which weighed samples were used. In addition, a proper diagnosis of BV was obtained using Gram-stained smears of the Vaginal fluid according to the method of Nugent et al. (R. P. Nugent et al., J Clin Microbiol 1991;29:297–301). We also diagnosed BV according to Hallen et al. (A. Hallen et al. Genitourin Med 1987;63:386–9). TMA was present in all women with a Nugent score between 7 and 10 and in almost all women diagnosed with BV according to the method of Hallen et al. TMA was not found or was only found in very low concentrations in Vaginal fluid from women with Nugent scores of 0 to 3. TMA was also found in four women with a negative sniff test. It seems that high levels of TMA in samples of Vaginal fluid are typical for BV regardless of the scoring method used for diagnosis. However, low levels of TMA, <5 μg/g Vaginal fluid, do not always correlate with BV.
Constanze Hartmann - One of the best experts on this subject based on the ideXlab platform.
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sperm activating odorous substances in human follicular fluid and Vaginal Secretion identification by gas chromatography olfactometry and ca2 imaging
ChemPlusChem, 2013Co-Authors: Constanze Hartmann, Annika Triller, Marc Spehr, Ralf Dittrich, Hanns Hatt, Andrea BuettnerAbstract:Human sperm chemotaxis to follicular fluid has been well established, but the molecular mechanism(s) for this phenomenon are still largely unclear. Studies indicate that odorant receptors expressed on spermatozoa could play a role in this scenario. It has recently been shown that several synthetic floral scents activate the receptors OR1D2, OR4D1, and OR7A5 in vitro and evoke distinct sperm motility patterns in vivo. However, all agonists found so far are of synthetic origin and, thus, the presence of endogenous structural analogues in female bodily fluids is subject to speculation. Therefore, the aim of the study reported herein was to investigate the occurrence of odor compounds in Vaginal Secretions and follicular fluid by using gas chromatography-olfactometry. Chemically identified constituents of either bodily fluid were then analyzed for activation of recombinant chemoreceptors and candidate ligands were further tested for induction of sperm Ca2+ signals. Through using this approach, two novel odorant receptor-ligand pairs are reported and human sperm Ca2+ elevations in response to both odorous substances, namely 5α-androst-16-en-3-one and 4-hydroxy-2,5-dimethyl-3(2H)-furanone, are shown.
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Sperm‐Activating Odorous Substances in Human Follicular Fluid and Vaginal Secretion: Identification by Gas Chromatography–Olfactometry and Ca2+ Imaging
ChemPlusChem, 2013Co-Authors: Constanze Hartmann, Annika Triller, Marc Spehr, Ralf Dittrich, Hanns Hatt, Andrea BuettnerAbstract:Human sperm chemotaxis to follicular fluid has been well established, but the molecular mechanism(s) for this phenomenon are still largely unclear. Studies indicate that odorant receptors expressed on spermatozoa could play a role in this scenario. It has recently been shown that several synthetic floral scents activate the receptors OR1D2, OR4D1, and OR7A5 in vitro and evoke distinct sperm motility patterns in vivo. However, all agonists found so far are of synthetic origin and, thus, the presence of endogenous structural analogues in female bodily fluids is subject to speculation. Therefore, the aim of the study reported herein was to investigate the occurrence of odor compounds in Vaginal Secretions and follicular fluid by using gas chromatography-olfactometry. Chemically identified constituents of either bodily fluid were then analyzed for activation of recombinant chemoreceptors and candidate ligands were further tested for induction of sperm Ca2+ signals. Through using this approach, two novel odorant receptor-ligand pairs are reported and human sperm Ca2+ elevations in response to both odorous substances, namely 5α-androst-16-en-3-one and 4-hydroxy-2,5-dimethyl-3(2H)-furanone, are shown.
Jacques Pillot - One of the best experts on this subject based on the ideXlab platform.
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Immunoglobulin G antibodies in human Vaginal Secretions after parenteral vaccination.
Infection and immunity, 1994Co-Authors: Jean-pierre Bouvet, Laurent Bélec, René Pires, Jacques PillotAbstract:The induction of antibodies in Vaginal Secretions by systemic (intramuscular) immunization in humans was investigated by using the tetanus toxoid vaccine. Five women, 30 to 40 years old, were injected with a currently used dose of toxoid (40 IU), and serum, saliva, and Vaginal Secretion samples were collected on day 0 and on day 6 or day 10. All of these subjects had been previously vaccinated at least 5 years before; four were in good health, whereas one suffered from AIDS in clinical category B3. In most cases, analysis of specific antibodies in the Vaginal wash showed a dramatic rise after boosting. These antibodies were primarily of the immunoglobulin G (IgG) isotype. The specific activity (ratio of antibody titer to IgG concentration) was shown to increase after the booster injection, irrespective of variations in the IgG level during the menstrual cycle. Comparison between serum and genital antibodies showed no difference in terms of both specific activity and level of avidity. These results demonstrate that parenteral injections can induce a systemic-derived antibody release in the Vaginal fluid. Hence, systemic vaccinations can be efficient at the genital level and thus could reinforce or even replace a local vaccine.
Helen Wolrath - One of the best experts on this subject based on the ideXlab platform.
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trimethylamine and trimethylamine oxide levels in normal women and women with bacterial vaginosis reflect a local metabolism in Vaginal Secretion as compared to urine
Apmis, 2005Co-Authors: Helen Wolrath, Anders Hallen, Bengt Stahlbom, Urban ForsumAbstract:The smell of rotten fish is one of the characteristics of bacterial vaginosis (BV), and is due to trimethylamine (TMA). Trimethylamine can be found in human urine, although most of it occurs as the nonvolatile oxide (TMAO) form. The fraction TMA/TMAO can be expected to be the same in different body fluids if no local production of TMA occurs. In women with BV, TMAO in the Vaginal fluid is expected to be chemically reduced by the local bacterial flora to the much more odorous TMA. We have therefore studied the local Vaginal production of TMA in Vaginal Secretion compared to the general TMA-TMAO metabolism that was measured in urine using gas chromatography. Both Vaginal fluid and random urine samples were collected from women, with and without BV, attending a Swedish clinic for sexually transmitted diseases, and these samples were analyzed for TMA and TMAO. The results show that a local production of TMA occurs in the vagina that is not part of the general metabolism of TMA-TMAO.
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trimethylamine content in Vaginal Secretion and its relation to bacterial vaginosis
Apmis, 2002Co-Authors: Helen Wolrath, Hans Boren, Anders Hallen, Urban ForsumAbstract:The presence of a fishy odor emanating from women who present with a malodorous Vaginal discharge is well known. The odor is due to bacterial reduction of trimethylamine oxide to trimethylamine (TMA) in Vaginal Secretion. The release of TMA from specimens of Vaginal fluid following the addition of alkali is often used in making a clinical diagnosis of bacterial vaginosis (BV). We now report a sensitive gas chromatographic method for analysis and quantification of TMA in Vaginal fluid in which weighed samples were used. In addition, a proper diagnosis of BV was obtained using Gram-stained smears of the Vaginal fluid according to the method of Nugent et al. (R. P. Nugent et al., J Clin Microbiol 1991;29:297–301). We also diagnosed BV according to Hallen et al. (A. Hallen et al. Genitourin Med 1987;63:386–9). TMA was present in all women with a Nugent score between 7 and 10 and in almost all women diagnosed with BV according to the method of Hallen et al. TMA was not found or was only found in very low concentrations in Vaginal fluid from women with Nugent scores of 0 to 3. TMA was also found in four women with a negative sniff test. It seems that high levels of TMA in samples of Vaginal fluid are typical for BV regardless of the scoring method used for diagnosis. However, low levels of TMA, <5 μg/g Vaginal fluid, do not always correlate with BV.
