The Experts below are selected from a list of 261 Experts worldwide ranked by ideXlab platform
Bart Nicolai - One of the best experts on this subject based on the ideXlab platform.
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the effect of sugar starvation on the metabolic response of isolated lamb s lettuce Valerianella locusta l cells
Acta Horticulturae, 2018Co-Authors: Baiye Mfortaw V Mbong, Maarten Hertog, Annemie Geeraerd, Bart NicolaiAbstract:Leafy vegetables are highly perishable as they are exposed to carbohydrate starvation during their postharvest storage life, leading to premature quality loss. An in-depth knowledge of the fundamental metabolic regulation and control mechanisms of these leafy vegetables to sugar starvation is beneficial in understanding how they modify their metabolism to survive in the absence of sugar. To this purpose, isolated lamb's lettuce cells were incubated in the dark to trigger sugar starvation at different temperatures (25, 18 and 1°C) to monitor the dynamic changes in the metabolome over time. To understand which pathways were up- or down-regulated, the isolated cells were preloaded with uniformly labelled 13C glucose and the distribution of the 13C label throughout the different metabolic pathways was monitored. The higher the temperature, moving from 1 to 25°C, the faster the effect of sugar starvation was induced.
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The effect of sugar starvation on the metabolic response of isolated lamb’s lettuce (Valerianella locusta L.) cells
Acta Horticulturae, 2018Co-Authors: Victor Baiye Mfortaw Mbong, Maarten Hertog, Annemie Geeraerd, Bart NicolaiAbstract:Leafy vegetables are highly perishable as they are exposed to carbohydrate starvation during their postharvest storage life, leading to premature quality loss. An in-depth knowledge of the fundamental metabolic regulation and control mechanisms of these leafy vegetables to sugar starvation is beneficial in understanding how they modify their metabolism to survive in the absence of sugar. To this purpose, isolated lamb's lettuce cells were incubated in the dark to trigger sugar starvation at different temperatures (25, 18 and 1°C) to monitor the dynamic changes in the metabolome over time. To understand which pathways were up- or down-regulated, the isolated cells were preloaded with uniformly labelled 13C glucose and the distribution of the 13C label throughout the different metabolic pathways was monitored. The higher the temperature, moving from 1 to 25°C, the faster the effect of sugar starvation was induced.
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the effect of temperature on the metabolic response of lamb s lettuce Valerianella locusta l laterr cells to sugar starvation
Postharvest Biology and Technology, 2017Co-Authors: Victor Baiye Mfortaw Mbong, Maarten Hertog, Annemie Geeraerd, Jerry Ampofoasiama, Bart NicolaiAbstract:Abstract Fresh leafy vegetables are highly perishable and may suffer from sugar starvation during postharvest storage. To fully understand their metabolic response to sugar starvation, isolated lamb’s lettuce ( Valerianella locusta, (L) Laterr.) cells were used as a model system to study biochemical and metabolic stress response to sugar starvation at 1 °C, 18 °C and 25 °C. The effect of sugar starvation was minimal at 1 °C. While the higher temperature showed clear impact of sugar starvation on the overall metabolic profile no significant differences were observed between the starvation at either 18 °C or 25 °C for the main sugars (glucose, glucose-6-phosphate, fructose, fructose-6-phosphate and sucrose). Biochemical and metabolic changes of the isolated cells upon sugar starvation involved a decrease in the levels of sugars, except for trehalose and ribose, as well as an increase in the levels of sugar alcohols. Sugar starvation altered the central metabolism by decreasing the levels of the intermediates of the glycolytic pathway, except for 3-phosphoglycerate and pyruvate. Increased levels of the intermediates of the tricarboxylic acid cycle were also observed. 13 C labelling data showed a decreased label accumulation in almost all metabolites, except for mannitol, myo-inositol, and trehalose. The increase in the levels of free soluble amino and fatty acids with a corresponding decrease in their 13 C label suggested a breakdown of protein and triacylglycerides.
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The effect of temperature on the metabolic response of lamb’s lettuce (Valerianella locusta, (L), Laterr.) cells to sugar starvation
Postharvest Biology and Technology, 2017Co-Authors: Victor Baiye Mfortaw Mbong, Jerry Ampofo-asiama, Maarten Hertog, Annemie Geeraerd, Bart NicolaiAbstract:Abstract Fresh leafy vegetables are highly perishable and may suffer from sugar starvation during postharvest storage. To fully understand their metabolic response to sugar starvation, isolated lamb’s lettuce ( Valerianella locusta, (L) Laterr.) cells were used as a model system to study biochemical and metabolic stress response to sugar starvation at 1 °C, 18 °C and 25 °C. The effect of sugar starvation was minimal at 1 °C. While the higher temperature showed clear impact of sugar starvation on the overall metabolic profile no significant differences were observed between the starvation at either 18 °C or 25 °C for the main sugars (glucose, glucose-6-phosphate, fructose, fructose-6-phosphate and sucrose). Biochemical and metabolic changes of the isolated cells upon sugar starvation involved a decrease in the levels of sugars, except for trehalose and ribose, as well as an increase in the levels of sugar alcohols. Sugar starvation altered the central metabolism by decreasing the levels of the intermediates of the glycolytic pathway, except for 3-phosphoglycerate and pyruvate. Increased levels of the intermediates of the tricarboxylic acid cycle were also observed. 13 C labelling data showed a decreased label accumulation in almost all metabolites, except for mannitol, myo-inositol, and trehalose. The increase in the levels of free soluble amino and fatty acids with a corresponding decrease in their 13 C label suggested a breakdown of protein and triacylglycerides.
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Metabolic profiling reveals a coordinated response of isolated lamb's (Valerianella locusta, L.) lettuce cells to sugar starvation and low oxygen stress
Postharvest Biology and Technology, 2017Co-Authors: Baiye Mfortaw Mbong Victor, Jerry Ampofo-asiama, Maarten Hertog, Annemie Geeraerd, Bart NicolaiAbstract:Sugar starvation is a common phenomenon occurring in most leafy vegetables after harvest and storage. Additionally, leafy vegetables are subjected to low O2 stress when stored in modified atmosphere conditions. In this study, the metabolism of isolated lamb’s lettuce cells was studied upon sugar starvation under O2 stress conditions, using 13C labelled glucose. Fast depletions of the soluble sugars were observed, being more pronounced under aerobic conditions than under low O2 stress conditions. Sugar starvation under aerobic conditions resulted in increased levels and decreased 13C label incorporation of TCA cycle intermediates and amino and fatty acids originating from glycolytic and TCA cycle pathways, compared to starving cells incubated under low O2 stress. On incubation under low O2 stress a switch in metabolism from aerobic to fermentation metabolism was observed. Under low O2 stress conditions, increased levels and 13C label incorporated in hexose phosphates, pyruvate, lactate, GABA, alanine, together with increased levels of acetaldehyde, ethanol and ethyl acetate was observed indicating fermentative metabolism was triggered.
M Gillis - One of the best experts on this subject based on the ideXlab platform.
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acidovorax Valerianellae sp nov a novel pathogen of lamb s lettuce Valerianella locusta l laterr
International Journal of Systematic and Evolutionary Microbiology, 2003Co-Authors: Louis Gardan, D E Stead, Catherine Dauga, M GillisAbstract:Bacterial spot disease of lamb’s lettuce [Valerianella locusta (L.) Laterr.] was first observed in fields in 1991. This new bacterial disease is localized in western France in high-technology field production of lamb’s lettuce for the preparation of ready-to-use salad. Nineteen strains isolated in 1992 and 1993 from typical black leaf spots of naturally infected lamb’s lettuce were characterized and compared with reference strains of Acidovorax and Delftia. The pathogenicity of the 19 strains was confirmed by artificial inoculation. Biochemical and physiological tests, fatty acid profiles, DNA–DNA hybridization and other nucleic acid-based tests were performed. A numerical taxonomic analysis of the 19 lamb’s lettuce strains showed a single homogeneous phenon closely related to previously described phytopathogenic taxa of the genus Acidovorax. DNA–DNA hybridization studies showed that the lamb’s lettuce strains were 91–100 % related to a representative strain, strain CFBP 4730 T , and constituted a discrete DNA hybridization group, indicating that they belong to the same novel species. Results from DNA–rRNA hybridization, 16S rRNA sequence analysis and fatty acid analysis studies confirmed that this novel species belongs to the b-subclass of the Proteobacteria and, more specifically, to the family Comamonadaceae and the genus Acidovorax. The name Acidovorax Valerianellae sp. nov. is proposed for this novel taxon of phytopathogenic bacteria. The type strain is strain CFBP 4730 T (=NCPPB 4283 T ). Lamb’s lettuce, also known as corn salad [Valerianella locusta (L.) Laterr.], was traditionally cultivated in France for use in salads in autumn and winter. Since 1985, advanced technology has been used for continuous, yearround cultivation; 90 % of French production (representing 75 % of world production) is concentrated in western France, near the Atlantic coast. The lettuce produced is used for sale traditionally, in trays, and for the preparation
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Acidovorax Valerianellae sp. nov., a novel pathogen of lamb's lettuce (Valerianella locusta (L.) Latter.)
International Journal of Systematic and Evolutionary Microbiology, 2003Co-Authors: L. Gardan, D E Stead, Catherine Dauga, M GillisAbstract:Bacterial spot disease of lamb's lettuce [Valerianella locusta (L.) Laterr.] was first observed in fields in 1991. This new bacterial disease is localized in western France in high-technology field production of lamb's lettuce for the preparation of ready-to-use salad. Nineteen strains isolated in 1992 and 1993 from typical black leaf spots of naturally infected lamb's lettuce were characterized and compared with reference strains of Acidovorax and Delftia. The pathogenicity of the 19 strains was confirmed by artificial inoculation. Biochemical and physiological tests, fatty acid profiles, DNA–DNA hybridization and other nucleic acid-based tests were performed. A numerical taxonomic analysis of the 19 lamb's lettuce strains showed a single homogeneous phenon closely related to previously described phytopathogenic taxa of the genus Acidovorax. DNA–DNA hybridization studies showed that the lamb's lettuce strains were 91–100 % related to a representative strain, strain CFBP 4730T, and constituted a discrete DNA hybridization group, indicating that they belong to the same novel species. Results from DNA–rRNA hybridization, 16S rRNA sequence analysis and fatty acid analysis studies confirmed that this novel species belongs to the β-subclass of the Proteobacteria and, more specifically, to the family Comamonadaceae and the genus Acidovorax. The name Acidovorax Valerianellae sp. nov. is proposed for this novel taxon of phytopathogenic bacteria. The type strain is strain CFBP 4730T (=NCPPB 4283T)
Annemie Geeraerd - One of the best experts on this subject based on the ideXlab platform.
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the effect of sugar starvation on the metabolic response of isolated lamb s lettuce Valerianella locusta l cells
Acta Horticulturae, 2018Co-Authors: Baiye Mfortaw V Mbong, Maarten Hertog, Annemie Geeraerd, Bart NicolaiAbstract:Leafy vegetables are highly perishable as they are exposed to carbohydrate starvation during their postharvest storage life, leading to premature quality loss. An in-depth knowledge of the fundamental metabolic regulation and control mechanisms of these leafy vegetables to sugar starvation is beneficial in understanding how they modify their metabolism to survive in the absence of sugar. To this purpose, isolated lamb's lettuce cells were incubated in the dark to trigger sugar starvation at different temperatures (25, 18 and 1°C) to monitor the dynamic changes in the metabolome over time. To understand which pathways were up- or down-regulated, the isolated cells were preloaded with uniformly labelled 13C glucose and the distribution of the 13C label throughout the different metabolic pathways was monitored. The higher the temperature, moving from 1 to 25°C, the faster the effect of sugar starvation was induced.
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The effect of sugar starvation on the metabolic response of isolated lamb’s lettuce (Valerianella locusta L.) cells
Acta Horticulturae, 2018Co-Authors: Victor Baiye Mfortaw Mbong, Maarten Hertog, Annemie Geeraerd, Bart NicolaiAbstract:Leafy vegetables are highly perishable as they are exposed to carbohydrate starvation during their postharvest storage life, leading to premature quality loss. An in-depth knowledge of the fundamental metabolic regulation and control mechanisms of these leafy vegetables to sugar starvation is beneficial in understanding how they modify their metabolism to survive in the absence of sugar. To this purpose, isolated lamb's lettuce cells were incubated in the dark to trigger sugar starvation at different temperatures (25, 18 and 1°C) to monitor the dynamic changes in the metabolome over time. To understand which pathways were up- or down-regulated, the isolated cells were preloaded with uniformly labelled 13C glucose and the distribution of the 13C label throughout the different metabolic pathways was monitored. The higher the temperature, moving from 1 to 25°C, the faster the effect of sugar starvation was induced.
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the effect of temperature on the metabolic response of lamb s lettuce Valerianella locusta l laterr cells to sugar starvation
Postharvest Biology and Technology, 2017Co-Authors: Victor Baiye Mfortaw Mbong, Maarten Hertog, Annemie Geeraerd, Jerry Ampofoasiama, Bart NicolaiAbstract:Abstract Fresh leafy vegetables are highly perishable and may suffer from sugar starvation during postharvest storage. To fully understand their metabolic response to sugar starvation, isolated lamb’s lettuce ( Valerianella locusta, (L) Laterr.) cells were used as a model system to study biochemical and metabolic stress response to sugar starvation at 1 °C, 18 °C and 25 °C. The effect of sugar starvation was minimal at 1 °C. While the higher temperature showed clear impact of sugar starvation on the overall metabolic profile no significant differences were observed between the starvation at either 18 °C or 25 °C for the main sugars (glucose, glucose-6-phosphate, fructose, fructose-6-phosphate and sucrose). Biochemical and metabolic changes of the isolated cells upon sugar starvation involved a decrease in the levels of sugars, except for trehalose and ribose, as well as an increase in the levels of sugar alcohols. Sugar starvation altered the central metabolism by decreasing the levels of the intermediates of the glycolytic pathway, except for 3-phosphoglycerate and pyruvate. Increased levels of the intermediates of the tricarboxylic acid cycle were also observed. 13 C labelling data showed a decreased label accumulation in almost all metabolites, except for mannitol, myo-inositol, and trehalose. The increase in the levels of free soluble amino and fatty acids with a corresponding decrease in their 13 C label suggested a breakdown of protein and triacylglycerides.
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The effect of temperature on the metabolic response of lamb’s lettuce (Valerianella locusta, (L), Laterr.) cells to sugar starvation
Postharvest Biology and Technology, 2017Co-Authors: Victor Baiye Mfortaw Mbong, Jerry Ampofo-asiama, Maarten Hertog, Annemie Geeraerd, Bart NicolaiAbstract:Abstract Fresh leafy vegetables are highly perishable and may suffer from sugar starvation during postharvest storage. To fully understand their metabolic response to sugar starvation, isolated lamb’s lettuce ( Valerianella locusta, (L) Laterr.) cells were used as a model system to study biochemical and metabolic stress response to sugar starvation at 1 °C, 18 °C and 25 °C. The effect of sugar starvation was minimal at 1 °C. While the higher temperature showed clear impact of sugar starvation on the overall metabolic profile no significant differences were observed between the starvation at either 18 °C or 25 °C for the main sugars (glucose, glucose-6-phosphate, fructose, fructose-6-phosphate and sucrose). Biochemical and metabolic changes of the isolated cells upon sugar starvation involved a decrease in the levels of sugars, except for trehalose and ribose, as well as an increase in the levels of sugar alcohols. Sugar starvation altered the central metabolism by decreasing the levels of the intermediates of the glycolytic pathway, except for 3-phosphoglycerate and pyruvate. Increased levels of the intermediates of the tricarboxylic acid cycle were also observed. 13 C labelling data showed a decreased label accumulation in almost all metabolites, except for mannitol, myo-inositol, and trehalose. The increase in the levels of free soluble amino and fatty acids with a corresponding decrease in their 13 C label suggested a breakdown of protein and triacylglycerides.
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Metabolic profiling reveals a coordinated response of isolated lamb's (Valerianella locusta, L.) lettuce cells to sugar starvation and low oxygen stress
Postharvest Biology and Technology, 2017Co-Authors: Baiye Mfortaw Mbong Victor, Jerry Ampofo-asiama, Maarten Hertog, Annemie Geeraerd, Bart NicolaiAbstract:Sugar starvation is a common phenomenon occurring in most leafy vegetables after harvest and storage. Additionally, leafy vegetables are subjected to low O2 stress when stored in modified atmosphere conditions. In this study, the metabolism of isolated lamb’s lettuce cells was studied upon sugar starvation under O2 stress conditions, using 13C labelled glucose. Fast depletions of the soluble sugars were observed, being more pronounced under aerobic conditions than under low O2 stress conditions. Sugar starvation under aerobic conditions resulted in increased levels and decreased 13C label incorporation of TCA cycle intermediates and amino and fatty acids originating from glycolytic and TCA cycle pathways, compared to starving cells incubated under low O2 stress. On incubation under low O2 stress a switch in metabolism from aerobic to fermentation metabolism was observed. Under low O2 stress conditions, increased levels and 13C label incorporated in hexose phosphates, pyruvate, lactate, GABA, alanine, together with increased levels of acetaldehyde, ethanol and ethyl acetate was observed indicating fermentative metabolism was triggered.
Maarten Hertog - One of the best experts on this subject based on the ideXlab platform.
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the effect of sugar starvation on the metabolic response of isolated lamb s lettuce Valerianella locusta l cells
Acta Horticulturae, 2018Co-Authors: Baiye Mfortaw V Mbong, Maarten Hertog, Annemie Geeraerd, Bart NicolaiAbstract:Leafy vegetables are highly perishable as they are exposed to carbohydrate starvation during their postharvest storage life, leading to premature quality loss. An in-depth knowledge of the fundamental metabolic regulation and control mechanisms of these leafy vegetables to sugar starvation is beneficial in understanding how they modify their metabolism to survive in the absence of sugar. To this purpose, isolated lamb's lettuce cells were incubated in the dark to trigger sugar starvation at different temperatures (25, 18 and 1°C) to monitor the dynamic changes in the metabolome over time. To understand which pathways were up- or down-regulated, the isolated cells were preloaded with uniformly labelled 13C glucose and the distribution of the 13C label throughout the different metabolic pathways was monitored. The higher the temperature, moving from 1 to 25°C, the faster the effect of sugar starvation was induced.
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The effect of sugar starvation on the metabolic response of isolated lamb’s lettuce (Valerianella locusta L.) cells
Acta Horticulturae, 2018Co-Authors: Victor Baiye Mfortaw Mbong, Maarten Hertog, Annemie Geeraerd, Bart NicolaiAbstract:Leafy vegetables are highly perishable as they are exposed to carbohydrate starvation during their postharvest storage life, leading to premature quality loss. An in-depth knowledge of the fundamental metabolic regulation and control mechanisms of these leafy vegetables to sugar starvation is beneficial in understanding how they modify their metabolism to survive in the absence of sugar. To this purpose, isolated lamb's lettuce cells were incubated in the dark to trigger sugar starvation at different temperatures (25, 18 and 1°C) to monitor the dynamic changes in the metabolome over time. To understand which pathways were up- or down-regulated, the isolated cells were preloaded with uniformly labelled 13C glucose and the distribution of the 13C label throughout the different metabolic pathways was monitored. The higher the temperature, moving from 1 to 25°C, the faster the effect of sugar starvation was induced.
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the effect of temperature on the metabolic response of lamb s lettuce Valerianella locusta l laterr cells to sugar starvation
Postharvest Biology and Technology, 2017Co-Authors: Victor Baiye Mfortaw Mbong, Maarten Hertog, Annemie Geeraerd, Jerry Ampofoasiama, Bart NicolaiAbstract:Abstract Fresh leafy vegetables are highly perishable and may suffer from sugar starvation during postharvest storage. To fully understand their metabolic response to sugar starvation, isolated lamb’s lettuce ( Valerianella locusta, (L) Laterr.) cells were used as a model system to study biochemical and metabolic stress response to sugar starvation at 1 °C, 18 °C and 25 °C. The effect of sugar starvation was minimal at 1 °C. While the higher temperature showed clear impact of sugar starvation on the overall metabolic profile no significant differences were observed between the starvation at either 18 °C or 25 °C for the main sugars (glucose, glucose-6-phosphate, fructose, fructose-6-phosphate and sucrose). Biochemical and metabolic changes of the isolated cells upon sugar starvation involved a decrease in the levels of sugars, except for trehalose and ribose, as well as an increase in the levels of sugar alcohols. Sugar starvation altered the central metabolism by decreasing the levels of the intermediates of the glycolytic pathway, except for 3-phosphoglycerate and pyruvate. Increased levels of the intermediates of the tricarboxylic acid cycle were also observed. 13 C labelling data showed a decreased label accumulation in almost all metabolites, except for mannitol, myo-inositol, and trehalose. The increase in the levels of free soluble amino and fatty acids with a corresponding decrease in their 13 C label suggested a breakdown of protein and triacylglycerides.
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The effect of temperature on the metabolic response of lamb’s lettuce (Valerianella locusta, (L), Laterr.) cells to sugar starvation
Postharvest Biology and Technology, 2017Co-Authors: Victor Baiye Mfortaw Mbong, Jerry Ampofo-asiama, Maarten Hertog, Annemie Geeraerd, Bart NicolaiAbstract:Abstract Fresh leafy vegetables are highly perishable and may suffer from sugar starvation during postharvest storage. To fully understand their metabolic response to sugar starvation, isolated lamb’s lettuce ( Valerianella locusta, (L) Laterr.) cells were used as a model system to study biochemical and metabolic stress response to sugar starvation at 1 °C, 18 °C and 25 °C. The effect of sugar starvation was minimal at 1 °C. While the higher temperature showed clear impact of sugar starvation on the overall metabolic profile no significant differences were observed between the starvation at either 18 °C or 25 °C for the main sugars (glucose, glucose-6-phosphate, fructose, fructose-6-phosphate and sucrose). Biochemical and metabolic changes of the isolated cells upon sugar starvation involved a decrease in the levels of sugars, except for trehalose and ribose, as well as an increase in the levels of sugar alcohols. Sugar starvation altered the central metabolism by decreasing the levels of the intermediates of the glycolytic pathway, except for 3-phosphoglycerate and pyruvate. Increased levels of the intermediates of the tricarboxylic acid cycle were also observed. 13 C labelling data showed a decreased label accumulation in almost all metabolites, except for mannitol, myo-inositol, and trehalose. The increase in the levels of free soluble amino and fatty acids with a corresponding decrease in their 13 C label suggested a breakdown of protein and triacylglycerides.
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Metabolic profiling reveals a coordinated response of isolated lamb's (Valerianella locusta, L.) lettuce cells to sugar starvation and low oxygen stress
Postharvest Biology and Technology, 2017Co-Authors: Baiye Mfortaw Mbong Victor, Jerry Ampofo-asiama, Maarten Hertog, Annemie Geeraerd, Bart NicolaiAbstract:Sugar starvation is a common phenomenon occurring in most leafy vegetables after harvest and storage. Additionally, leafy vegetables are subjected to low O2 stress when stored in modified atmosphere conditions. In this study, the metabolism of isolated lamb’s lettuce cells was studied upon sugar starvation under O2 stress conditions, using 13C labelled glucose. Fast depletions of the soluble sugars were observed, being more pronounced under aerobic conditions than under low O2 stress conditions. Sugar starvation under aerobic conditions resulted in increased levels and decreased 13C label incorporation of TCA cycle intermediates and amino and fatty acids originating from glycolytic and TCA cycle pathways, compared to starving cells incubated under low O2 stress. On incubation under low O2 stress a switch in metabolism from aerobic to fermentation metabolism was observed. Under low O2 stress conditions, increased levels and 13C label incorporated in hexose phosphates, pyruvate, lactate, GABA, alanine, together with increased levels of acetaldehyde, ethanol and ethyl acetate was observed indicating fermentative metabolism was triggered.
Victor Baiye Mfortaw Mbong - One of the best experts on this subject based on the ideXlab platform.
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The effect of sugar starvation on the metabolic response of isolated lamb’s lettuce (Valerianella locusta L.) cells
Acta Horticulturae, 2018Co-Authors: Victor Baiye Mfortaw Mbong, Maarten Hertog, Annemie Geeraerd, Bart NicolaiAbstract:Leafy vegetables are highly perishable as they are exposed to carbohydrate starvation during their postharvest storage life, leading to premature quality loss. An in-depth knowledge of the fundamental metabolic regulation and control mechanisms of these leafy vegetables to sugar starvation is beneficial in understanding how they modify their metabolism to survive in the absence of sugar. To this purpose, isolated lamb's lettuce cells were incubated in the dark to trigger sugar starvation at different temperatures (25, 18 and 1°C) to monitor the dynamic changes in the metabolome over time. To understand which pathways were up- or down-regulated, the isolated cells were preloaded with uniformly labelled 13C glucose and the distribution of the 13C label throughout the different metabolic pathways was monitored. The higher the temperature, moving from 1 to 25°C, the faster the effect of sugar starvation was induced.
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the effect of temperature on the metabolic response of lamb s lettuce Valerianella locusta l laterr cells to sugar starvation
Postharvest Biology and Technology, 2017Co-Authors: Victor Baiye Mfortaw Mbong, Maarten Hertog, Annemie Geeraerd, Jerry Ampofoasiama, Bart NicolaiAbstract:Abstract Fresh leafy vegetables are highly perishable and may suffer from sugar starvation during postharvest storage. To fully understand their metabolic response to sugar starvation, isolated lamb’s lettuce ( Valerianella locusta, (L) Laterr.) cells were used as a model system to study biochemical and metabolic stress response to sugar starvation at 1 °C, 18 °C and 25 °C. The effect of sugar starvation was minimal at 1 °C. While the higher temperature showed clear impact of sugar starvation on the overall metabolic profile no significant differences were observed between the starvation at either 18 °C or 25 °C for the main sugars (glucose, glucose-6-phosphate, fructose, fructose-6-phosphate and sucrose). Biochemical and metabolic changes of the isolated cells upon sugar starvation involved a decrease in the levels of sugars, except for trehalose and ribose, as well as an increase in the levels of sugar alcohols. Sugar starvation altered the central metabolism by decreasing the levels of the intermediates of the glycolytic pathway, except for 3-phosphoglycerate and pyruvate. Increased levels of the intermediates of the tricarboxylic acid cycle were also observed. 13 C labelling data showed a decreased label accumulation in almost all metabolites, except for mannitol, myo-inositol, and trehalose. The increase in the levels of free soluble amino and fatty acids with a corresponding decrease in their 13 C label suggested a breakdown of protein and triacylglycerides.
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The effect of temperature on the metabolic response of lamb’s lettuce (Valerianella locusta, (L), Laterr.) cells to sugar starvation
Postharvest Biology and Technology, 2017Co-Authors: Victor Baiye Mfortaw Mbong, Jerry Ampofo-asiama, Maarten Hertog, Annemie Geeraerd, Bart NicolaiAbstract:Abstract Fresh leafy vegetables are highly perishable and may suffer from sugar starvation during postharvest storage. To fully understand their metabolic response to sugar starvation, isolated lamb’s lettuce ( Valerianella locusta, (L) Laterr.) cells were used as a model system to study biochemical and metabolic stress response to sugar starvation at 1 °C, 18 °C and 25 °C. The effect of sugar starvation was minimal at 1 °C. While the higher temperature showed clear impact of sugar starvation on the overall metabolic profile no significant differences were observed between the starvation at either 18 °C or 25 °C for the main sugars (glucose, glucose-6-phosphate, fructose, fructose-6-phosphate and sucrose). Biochemical and metabolic changes of the isolated cells upon sugar starvation involved a decrease in the levels of sugars, except for trehalose and ribose, as well as an increase in the levels of sugar alcohols. Sugar starvation altered the central metabolism by decreasing the levels of the intermediates of the glycolytic pathway, except for 3-phosphoglycerate and pyruvate. Increased levels of the intermediates of the tricarboxylic acid cycle were also observed. 13 C labelling data showed a decreased label accumulation in almost all metabolites, except for mannitol, myo-inositol, and trehalose. The increase in the levels of free soluble amino and fatty acids with a corresponding decrease in their 13 C label suggested a breakdown of protein and triacylglycerides.
