The Experts below are selected from a list of 186 Experts worldwide ranked by ideXlab platform
Geoffrey Burnstock - One of the best experts on this subject based on the ideXlab platform.
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Reinnervation of transplanted Vas Deferens by cholinergic nerves normally supplying skeletal muscle
Experimental Neurology, 2008Co-Authors: Gerta Vrbová, Geoffrey BurnstockAbstract:The rat Vas Deferens was removed and either transplanted alongside the soleus muscle or into the bed of the soleus muscle that had previously been removed, and in this case the soleus nerve was connected to the transplant. The Vas Deferens reinnervated by the somatomotor nerve recovered the best. Contractions to transmural electrical stimulation could not be elicited from the denervated Vas Deferens, although noradrenaline and acetylcholine elicited contractions. The reinnervated Vas Deferens produced good contractile responses to transmural stimulation, and these were substantially reduced by a cholinergic muscarinic blocking agent, hyoscine, as compared to only a small reduction in the control Vas Deferens. Neostigmine potentiated the contraction of the transplanted Vas Deferens to a greater extent than that of the control. This indicated that a substantial component of the contractile response was produced by cholinergic fibres. Consistent with this was the finding that, while guanethidine blocked a greater proportion of the contraction in the control Vas Deferens, the contraction of the reinnervated transplant was less affected. Acetylcholine elicited a strong contraction in control Vas Deferens, but only a small response was obtained in the reinnervated transplant. However, the response to noradrenaline was greater in the transplant than in the control Vas Deferens. These results indicate that cholinergic nerves normally supplying skeletal muscle can reinnervate smooth muscle and that the alien somatomotor innervation altered the responsiveness of the smooth muscle of the Vas Deferens. Morphological studies confirm the shift from adrenergic to cholinergic fibres in the reinnervated Vas Deferens.
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the purinergic component of human Vas Deferens contraction
Fertility and Sterility, 2006Co-Authors: Frederick C L Banks, Gillian E Knight, Robert C Calvert, C S Thompson, R J Morgan, Geoffrey BurnstockAbstract:Objective To examine purinergic signaling in human Vas Deferens. Design To study contractile responses of the scrotal Vas Deferens. Setting Research department of a university teaching hospital. Patient(s) Undergoing Vasectomy or orchidectomy (aged 27–88 years, n=14). Intervention(s) Vasectomy or orchidectomy. Main Outcome Measure(s) Strips of Vas Deferens were suspended in an organ bath and subjected to electrical stimulation to establish frequency–response curves. These stimulations were repeated in the presence of pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid (PPADS, P2 receptor antagonist), prazosin (adrenergic α 1 antagonist), and tetrodotoxin. Concentration–response curves were constructed to noradrenaline and the P2X agonists ATP and α,β-methylene ATP (α,β-meATP). The P2X receptor subtype distribution was assessed by immunohistochemistry using specific antibodies. Result(s) The response at 32 Hz in the presence of PPADS was reduced by 40% and in the presence of prazosin by 80%. Noradrenaline caused concentration-dependent contractions (EC 50 = 11.8 μM). Contractions to ATP and α,β-meATP (EC 50 = 6.27 μM) suggested that the functional receptor was P2X 1 and/or P2X 3 . However, immunohistochemistry demonstrated P2X 1 , but not P2X 3 , receptor immunoreactivity on the smooth muscle cells. Conclusion(s) This study demonstrated that ATP is a co-transmitter with noradrenaline in the contraction of the human Vas Deferens predominantly acting through the P2X 1 receptor.
Frederick C L Banks - One of the best experts on this subject based on the ideXlab platform.
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the purinergic component of human Vas Deferens contraction
Fertility and Sterility, 2006Co-Authors: Frederick C L Banks, Gillian E Knight, Robert C Calvert, C S Thompson, R J Morgan, Geoffrey BurnstockAbstract:Objective To examine purinergic signaling in human Vas Deferens. Design To study contractile responses of the scrotal Vas Deferens. Setting Research department of a university teaching hospital. Patient(s) Undergoing Vasectomy or orchidectomy (aged 27–88 years, n=14). Intervention(s) Vasectomy or orchidectomy. Main Outcome Measure(s) Strips of Vas Deferens were suspended in an organ bath and subjected to electrical stimulation to establish frequency–response curves. These stimulations were repeated in the presence of pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid (PPADS, P2 receptor antagonist), prazosin (adrenergic α 1 antagonist), and tetrodotoxin. Concentration–response curves were constructed to noradrenaline and the P2X agonists ATP and α,β-methylene ATP (α,β-meATP). The P2X receptor subtype distribution was assessed by immunohistochemistry using specific antibodies. Result(s) The response at 32 Hz in the presence of PPADS was reduced by 40% and in the presence of prazosin by 80%. Noradrenaline caused concentration-dependent contractions (EC 50 = 11.8 μM). Contractions to ATP and α,β-meATP (EC 50 = 6.27 μM) suggested that the functional receptor was P2X 1 and/or P2X 3 . However, immunohistochemistry demonstrated P2X 1 , but not P2X 3 , receptor immunoreactivity on the smooth muscle cells. Conclusion(s) This study demonstrated that ATP is a co-transmitter with noradrenaline in the contraction of the human Vas Deferens predominantly acting through the P2X 1 receptor.
Maria Fatima M Lazari - One of the best experts on this subject based on the ideXlab platform.
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locally produced relaxin may affect testis and Vas Deferens function in rats
Reproduction, 2010Co-Authors: Lais Cavalca Cardoso, Aline R Nascimento, Carine Royer, Catarina S Porto, Maria Fatima M LazariAbstract:: We have previously shown that the rat testis and Vas Deferens contain high levels of the relaxin receptor, RXFP1. The present study was undertaken to determine the expression of relaxin in these tissues, and the effect of exogenous relaxin on Sertoli cell proliferation and on the mRNA levels of some proteins that may contribute to epithelial secretion and tissue reorganization in the Vas Deferens. Relaxin mRNA levels in testis and Vas Deferens were much lower than in the prostate. Sertoli cells seem to be an important source of relaxin mRNA in testis. Relaxin immunoreactivity was detected in the seminiferous epithelium but not in the interstitial compartment. The relaxin precursor was expressed in the Vas Deferens, and relaxin immunoreactivity was detected in apical cells of the Vas Deferens. Castration, but not treatment with the anti-estrogen ICI 182,780, dramatically reduced relaxin mRNA levels in the prostate and Vas Deferens, and this effect was prevented by testosterone. Rxfp1 mRNA levels in the Vas Deferens and prostate were not affected by castration or treatment with ICI 182,780. Exogenous relaxin increased the incorporation of (3)H-thymidine in cultured Sertoli cells, and treatment of the Vas Deferens with 100 ng/ml relaxin increased the mRNA levels for the cystic fibrosis chloride channel (cystic fibrosis transmembrane regulator) about three times, and doubled mRNA levels for the inducible form of nitric oxide synthase and metalloproteinase 7. These results suggest that locally produced relaxin acts as an autocrine or paracrine agent in the testis and Vas Deferens to affect spermatogenesis and seminal fluid composition.
Robert C Calvert - One of the best experts on this subject based on the ideXlab platform.
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the purinergic component of human Vas Deferens contraction
Fertility and Sterility, 2006Co-Authors: Frederick C L Banks, Gillian E Knight, Robert C Calvert, C S Thompson, R J Morgan, Geoffrey BurnstockAbstract:Objective To examine purinergic signaling in human Vas Deferens. Design To study contractile responses of the scrotal Vas Deferens. Setting Research department of a university teaching hospital. Patient(s) Undergoing Vasectomy or orchidectomy (aged 27–88 years, n=14). Intervention(s) Vasectomy or orchidectomy. Main Outcome Measure(s) Strips of Vas Deferens were suspended in an organ bath and subjected to electrical stimulation to establish frequency–response curves. These stimulations were repeated in the presence of pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid (PPADS, P2 receptor antagonist), prazosin (adrenergic α 1 antagonist), and tetrodotoxin. Concentration–response curves were constructed to noradrenaline and the P2X agonists ATP and α,β-methylene ATP (α,β-meATP). The P2X receptor subtype distribution was assessed by immunohistochemistry using specific antibodies. Result(s) The response at 32 Hz in the presence of PPADS was reduced by 40% and in the presence of prazosin by 80%. Noradrenaline caused concentration-dependent contractions (EC 50 = 11.8 μM). Contractions to ATP and α,β-meATP (EC 50 = 6.27 μM) suggested that the functional receptor was P2X 1 and/or P2X 3 . However, immunohistochemistry demonstrated P2X 1 , but not P2X 3 , receptor immunoreactivity on the smooth muscle cells. Conclusion(s) This study demonstrated that ATP is a co-transmitter with noradrenaline in the contraction of the human Vas Deferens predominantly acting through the P2X 1 receptor.
C S Thompson - One of the best experts on this subject based on the ideXlab platform.
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the purinergic component of human Vas Deferens contraction
Fertility and Sterility, 2006Co-Authors: Frederick C L Banks, Gillian E Knight, Robert C Calvert, C S Thompson, R J Morgan, Geoffrey BurnstockAbstract:Objective To examine purinergic signaling in human Vas Deferens. Design To study contractile responses of the scrotal Vas Deferens. Setting Research department of a university teaching hospital. Patient(s) Undergoing Vasectomy or orchidectomy (aged 27–88 years, n=14). Intervention(s) Vasectomy or orchidectomy. Main Outcome Measure(s) Strips of Vas Deferens were suspended in an organ bath and subjected to electrical stimulation to establish frequency–response curves. These stimulations were repeated in the presence of pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid (PPADS, P2 receptor antagonist), prazosin (adrenergic α 1 antagonist), and tetrodotoxin. Concentration–response curves were constructed to noradrenaline and the P2X agonists ATP and α,β-methylene ATP (α,β-meATP). The P2X receptor subtype distribution was assessed by immunohistochemistry using specific antibodies. Result(s) The response at 32 Hz in the presence of PPADS was reduced by 40% and in the presence of prazosin by 80%. Noradrenaline caused concentration-dependent contractions (EC 50 = 11.8 μM). Contractions to ATP and α,β-meATP (EC 50 = 6.27 μM) suggested that the functional receptor was P2X 1 and/or P2X 3 . However, immunohistochemistry demonstrated P2X 1 , but not P2X 3 , receptor immunoreactivity on the smooth muscle cells. Conclusion(s) This study demonstrated that ATP is a co-transmitter with noradrenaline in the contraction of the human Vas Deferens predominantly acting through the P2X 1 receptor.
