The Experts below are selected from a list of 312 Experts worldwide ranked by ideXlab platform
Caihuan Ke - One of the best experts on this subject based on the ideXlab platform.
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Proteomic analysis of trochophore and Veliger larvae development in the small abalone Haliotis diversicolor
BMC Genomics, 2017Co-Authors: Guilan Di, Xiulian Miao, Miaoqin Huang, Weiwei You, Yifang Zhang, Yuting Gu, Xianghui Kong, Jianxin Zhang, Caihuan KeAbstract:BackgroundHaliotis diversicolor is commercially important species. The trochophore and Veliger are distinct larval stages in gastropod development. Their development involves complex morphological and physiological changes. We studied protein changes during the embryonic development of H. diversicolor using two dimensional electrophoresis (2-DE) and label-free methods, tandem mass spectrometry (MS/ MS), and Mascot for protein identification.ResultsA total of 150 2-DE gel spots were identified. Protein spots showed upregulation of 15 proteins and downregulation of 28 proteins as H. diversicolor developed from trochophore to Veliger larvae. Trochophore and Veliger larvae were compared using a label-free quantitative proteomic approach. A total of 526 proteins were identified from both samples, and 104 proteins were differentially expressed (> 1.5 fold). Compared with trochophore larvae, Veliger larvae had 55 proteins upregulated and 49 proteins downregulated. These differentially expressed proteins were involved in shell formation, energy metabolism, cellular and stress response processes, protein synthesis and folding, cell cycle, and cell fate determination. Compared with the 5 protein (fructose-bisphosphate aldolase, 14–3-3ε, profilin, actin-depolymerizing factor (ADF)/cofilin) and calreticulin) expression patterns, the mRNA expression exhibited similar patterns except gene of fructose-bisphosphate aldolase.ConclusionOur results provide insight into novel aspects of protein function in shell formation, torsion, and nervous system development, and muscle system differentiation in H. diversicolor larvae. “Quality control” proteins were identified to be involved in abalone larval development.
Catherine Kilduff - One of the best experts on this subject based on the ideXlab platform.
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Rapana venosa as an indicator species for TBT exposure over decadal and seasonal scales
Marine Biology, 2013Co-Authors: Juliana M. Harding, Michael A. Unger, Roger Mann, E. Alex Jestel, Catherine KilduffAbstract:Imposex and decline in reproductive output in marine gastropods have been linked to tributyltin (TBT) exposure. This study describes Chesapeake Bay, USA, veined rapa whelk Rapana venosa imposex incidence and sex ratios from 1998 to 2009. Tissue TBT concentrations (ng g^−1) were examined with respect to whelk sex, size, and water temperature at the time of collection, and also to egg case size, hatching success, and Veliger diameter. Imposex incidence declined and population sex ratios moved closer to parity from 1998 to 2009. Observed TBT concentrations (ng g^−1) were higher in James River than in Ocean View whelks with higher TBT concentrations observed in males than in females. Exponential declines in TBT concentrations from female-specific first to last clutches within a reproductive season were observed, indicating that whelks depurate TBT through egg case deposition. Egg capsule hatching success and Veliger size were similar for female and imposex whelks. The R. venosa imposex levels observed in Chesapeake Bay apparently do not affect the production, release, or viability of larvae.
Guilan Di - One of the best experts on this subject based on the ideXlab platform.
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Proteomic analysis of trochophore and Veliger larvae development in the small abalone Haliotis diversicolor
BMC Genomics, 2017Co-Authors: Guilan Di, Xiulian Miao, Miaoqin Huang, Weiwei You, Yifang Zhang, Yuting Gu, Xianghui Kong, Jianxin Zhang, Caihuan KeAbstract:BackgroundHaliotis diversicolor is commercially important species. The trochophore and Veliger are distinct larval stages in gastropod development. Their development involves complex morphological and physiological changes. We studied protein changes during the embryonic development of H. diversicolor using two dimensional electrophoresis (2-DE) and label-free methods, tandem mass spectrometry (MS/ MS), and Mascot for protein identification.ResultsA total of 150 2-DE gel spots were identified. Protein spots showed upregulation of 15 proteins and downregulation of 28 proteins as H. diversicolor developed from trochophore to Veliger larvae. Trochophore and Veliger larvae were compared using a label-free quantitative proteomic approach. A total of 526 proteins were identified from both samples, and 104 proteins were differentially expressed (> 1.5 fold). Compared with trochophore larvae, Veliger larvae had 55 proteins upregulated and 49 proteins downregulated. These differentially expressed proteins were involved in shell formation, energy metabolism, cellular and stress response processes, protein synthesis and folding, cell cycle, and cell fate determination. Compared with the 5 protein (fructose-bisphosphate aldolase, 14–3-3ε, profilin, actin-depolymerizing factor (ADF)/cofilin) and calreticulin) expression patterns, the mRNA expression exhibited similar patterns except gene of fructose-bisphosphate aldolase.ConclusionOur results provide insight into novel aspects of protein function in shell formation, torsion, and nervous system development, and muscle system differentiation in H. diversicolor larvae. “Quality control” proteins were identified to be involved in abalone larval development.
K E Caihuan - One of the best experts on this subject based on the ideXlab platform.
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acute toxicity test of formaldehyde and tributyltin to babylonia areolata Veliger larvae
Journal of Jimei University, 2007Co-Authors: K E CaihuanAbstract:Acute toxicity test of formaldehyde and tributyltin to Babylonia areolata Veliger larvae was studied.The experimental results showed that the 24,48,72,96 h LC50 values were 25.716,8.973,3.089,2.830 mg·L-1 for formaldehyde and 25.142,16.986,7.130,5.468 μg·L-1 for tributyltin.The safe concentration of formaldehyde and tributyltin to Babylonia areolata Veliger larvae was also estimated,0.283 mg·L-1 and 0.547 μg·L-1 respectively.
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acute toxicity tests of the lead and cadmium to babylonia areolate Veliger larvae
Journal of Zhangzhou Normal University, 2007Co-Authors: K E CaihuanAbstract:Acute toxicity test of lead(Pb2+) and cadmium(Cd2+) to Babylonia areolata Veliger larvae were studied in this research.The experimental results showed that after treatment of 48h、72h and 96h,LC50 values of lead were 19.547 mg.L-1,4.571 mg·L-1 and 1.071 mg·L-1respectively,and LC50 values of cadmium were 2.960 mg·L-1,0.660 mg·L-1 and 0.202 mg·L-1 respectively.The safe concentration of lead and cadmium to B.areolata Veliger larvae were also estimated to be 0.107 mg·L-1,0.020 mg·L-1 respectively.
Juliana M. Harding - One of the best experts on this subject based on the ideXlab platform.
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GROWTH AND DEVELOPMENT OF VEINED RAPA WHELK RAPANA VENOSA VeligerS
Journal of Shellfish Research, 2020Co-Authors: Juliana M. HardingAbstract:Abstract Planktonic larvae of benthic fauna that can grow quickly in the plankton and reduce their larval period duration lessen their exposure to pelagic predators and reduce the potential for advection away from suitable habitats. Veined rapa whelks (Rapana venosa, Muricidae) lay egg masses that release planktonic Veliger larvae from May through August in Chesapeake Bay, USA. Two groups of Veliger larvae hatched from egg masses during June and August 2000 were cultured in the laboratory. Egg mass incubation time (time from deposition to hatch) ranged from 18–26 d at water temperatures between 22°C and 27°C. Four stages of rapa whelk Veliger development along a time series from hatch to settlement were described using external morphological features. Rapa whelk Veligers were measured at three-day intervals from hatching through the onset of spontaneous settlement. A four-parameter Gompertz growth model was used with length at age data from cultures of rapa whelk Veligers to estimate maximum growth rates ...
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Ontogenetic changes in predator–prey interactions between two species of larval fishes and oyster Veligers
Journal of Experimental Marine Biology and Ecology, 2015Co-Authors: Juliana M. Harding, Dennis M. Allen, Sarah Dingley, Rachel M. Tremont, Stephanie M. Krug, C. Taylor ArmstrongAbstract:Abstract The naked goby ( Gobiosoma bosc ), striped blenny ( Chasmodes bosquianus ), and eastern oyster ( Crassostrea virginica ) are common resident species in temperate Atlantic tidal waters. All three species produce planktonic larvae, and interactions between their early life stages likely influence recruitment success. Fish predation on oyster Veligers requires co-occurrence as well as a match between prey behavior, size, and morphology and the ability of the predator to capture and engulf the Veliger (prey). Wild plankton, oyster Veligers, and wild plankton enriched with oyster Veligers (mixed) were fed to cultured larval fishes during laboratory experiments. Three hypotheses were tested: (1) no fish species-specific differences in oyster Veliger consumption exist within similar predator–prey development stage combinations, (2) postflexion blennies and gobies consume more Veligers than preflexion fishes, (3) Veligers reach a size refuge from fish predation by the umbo developmental stage. The resulting data provide a quantitative description of larval fish feeding behavior with respect to fish (preflexion, postflexion) and Veliger (straight hinge, umbo) developmental stage. Selectivity for bivalve Veligers as well as ontogenetic effects on the predator–prey interaction were examined. Fish nests were cultured to provide larval fish predators of known age. Overall, 70% of striped blennies and 59% of naked gobies consumed at least one prey item during the 3 h experiments. There were fish species-specific differences in oyster Veliger consumption between striped blennies and naked gobies. Striped blennies consumed more prey items including a wider range of Veliger ages than larval naked gobies of the same developmental stages, regardless of the prey treatment. Fish selectivity was high for copepod nauplii and bivalve Veligers and usually low for polychaete larvae, barnacle nauplii, and diatoms. There was a significant positive relationship between standardized consumption and predator development stage in the wild treatment for both fishes and in mixed and Veliger treatments for blennies. All fishes consumed straight hinge Veligers more often than umbo stage Veligers. The largest Veligers appear to reach a predation refuge from both fishes. Larval gobies and blennies are generalist predators that use seasonal pulses of zooplankton prey within temperate estuaries. Potential mismatches between predator–prey spatial and temporal overlap that might reduce fish recruitment are likely offset by adult spawning strategies that result in multiple nests within a long spawning season.
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Rapana venosa as an indicator species for TBT exposure over decadal and seasonal scales
Marine Biology, 2013Co-Authors: Juliana M. Harding, Michael A. Unger, Roger Mann, E. Alex Jestel, Catherine KilduffAbstract:Imposex and decline in reproductive output in marine gastropods have been linked to tributyltin (TBT) exposure. This study describes Chesapeake Bay, USA, veined rapa whelk Rapana venosa imposex incidence and sex ratios from 1998 to 2009. Tissue TBT concentrations (ng g^−1) were examined with respect to whelk sex, size, and water temperature at the time of collection, and also to egg case size, hatching success, and Veliger diameter. Imposex incidence declined and population sex ratios moved closer to parity from 1998 to 2009. Observed TBT concentrations (ng g^−1) were higher in James River than in Ocean View whelks with higher TBT concentrations observed in males than in females. Exponential declines in TBT concentrations from female-specific first to last clutches within a reproductive season were observed, indicating that whelks depurate TBT through egg case deposition. Egg capsule hatching success and Veliger size were similar for female and imposex whelks. The R. venosa imposex levels observed in Chesapeake Bay apparently do not affect the production, release, or viability of larvae.
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Selective feeding behavior of larval naked gobies Gobiosoma bosc and blennies Chasmodes bosquianus and Hypsoblennius hentzi : preferences for bivalve Veligers
Marine Ecology Progress Series, 1999Co-Authors: Juliana M. HardingAbstract:Naked gobies Gobiosoma bosc, striped blennies Chasmodes bosquianus, and feather blennies Hypsoblennius hentzi provide important intermediate links within the trophic structure of estuarine oyster reef communities. Predator-prey interactions between planktonic larvae of these fishes and larval eastern oysters Crassostrea virginica may influence recruitment success within oyster reef communities. These 3 species of oyster reef fish larvae were cultured from wild nests and used in multi-factorial laboratory feeding experiments with larval oysters or hard clams Mercenaria mercenaria as well as wild plankton as prey items to determine the effects of predator age, predator concentration, and prey type on feeding selectivity of these fishes. Predator age significantly influenced feeding behavior of naked gobies and feather blennies. Predator concentration did not significantly effect feeding behavior for any of the 3 fish species. Prey type significantly affected feeding behavior of feather blennies and naked gobies. Naked gobies consumed bivalve Veligers preferentially at all Veliger concentrations. Feather blennies consumed Veligers preferentially at concentrations as low as 12% of the available prey field. Striped blennies were less specialized in their feeding patterns but still consumed bivalve Veligers preferentially at prey field concentrations as low as 11% Veligers.