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Jianmin Zhao - One of the best experts on this subject based on the ideXlab platform.
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A macin identified from Venerupis philippinarum: Investigation on antibacterial activities and action mode.
Fish & Shellfish Immunology, 2019Co-Authors: Dinglong Yang, Qing Wang, Yijing Han, Lizhu Chen, Ruiwen Cao, Zhijun Dong, Hui Liu, Xiaoli Zhang, Qianqian Zhang, Jianmin ZhaoAbstract:In the present study, a macin was cloned and characterized from clam Venerupis philippinarum (designed as VpMacin). The full-length cDNA of VpMacin was of 579 bp, encoding a peptide of 87 amino acids with the predicted molecular weight of 9.7 kDa. Analysis of the conserved domain suggested that VpMacin was a new member of the macin family. In non-stimulated clams, VpMacin transcripts exhibited different tissue expression pattern, and highly expressed in the tissues of gills and hepatopancreas. Generally, the temporal expression of VpMacin transcripts was significantly induced in hemocytes of clams post Vibrio anguillarum challenge. Moreover, the recombinant VpMacin protein (rVpMacin) showed obvious antimicrobial activities against Gram-positive and Gram-negative bacteria. After incubated with 40 μM rVpMacin, all detected Escherichia coli could be killed within 60 min. Membrane integrity analysis revealed that rVpMacin could increase the membrane permeability of bacteria and then resulted in cell death. Overall, our results suggested that VpMacin had an important function in host defense against invasive pathogens.
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Molecular characterization of a Se-containing glutathione peroxidases gene and its expressions to heavy metals compared with non-Se-containing glutathione peroxidases in Venerupis philippinarum
Agri Gene, 2016Co-Authors: Ming Cong, Jianmin Zhao, Zhang Linbao, Lei Zhang, Haiqiang Chen, Junli KongAbstract:Abstract Heavy metal pollution is an increasing environmental problem around the coastline. As a kind of sedentary mollusk, Venerupis philippinarum is an important sentinel to survey the environmental quality of coastal flat. Glutathione peroxidases are important anti-oxidant enzymes to alleviate oxidative stress caused by heavy metals. In the present study, a new cDNA sequence encoding a Se-containing glutathione peroxidase (VpSeGPx2) was isolated from V. philippinarum. The full-length cDNA of VpSeGPx2 was 963 bp with a conserved selenocysteine insertion sequence (SECIS) in its 3′-UTR, encoding a polypeptide of 242 amino acids with a signal peptide of 19-amino acids. Phylogenetic analysis revealed that VpSeGPx2 was clustered with Se-GPx proteins from marine mollusks. VpSeGPx2 was found to be significantly down-regulated by 10 μg/L of copper and up-regulated by 40 μg/L of copper. However, cadmium exposure seemed to have no significant effect on the expression of VpSeGPx2 transcripts. As for a previously cloned non-Se-containing VpGPx, both concentrations of copper exposure (10 and 40 μg/L) significantly increased its mRNA expression, and a higher concentration (40 μg/L) of cadmium significantly inhibited the expression of VpGPx transcript. These results suggested that VpSeGPx2 and VpGPx were both involved in the detoxification of copper pollution but seemed to play a different role in cadmium pollution.
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Glutathione S-transferase (GST) gene expression profiles in two marine bivalves exposed to BDE-47 and their potential molecular mechanisms
Chinese Journal of Oceanology and Limnology, 2015Co-Authors: Qing Wang, Jianmin ZhaoAbstract:Glutathione S-transferases (GSTs) are phase II enzymes that facilitate the detoxification of xenobiotics and play important roles in antioxidant defense. We investigated the expression patterns of seven Venerupis philippinarum GSTs (VpGSTs) and four Mytilus galloprovincialis GSTs (MgGSTs) following exposure to BDE-47. Differential expressions of the seven VpGSTs and four Mg GSTs transcripts were observed, with differences between the hepatopancreas and gills. Among these GSTs, the sigma classes (VpGSTS1, VpGSTS2, VpGSTS3, MgGST1, and MgGST3) were highly expressed in response to BDE-47 exposure, demonstrating their potential as molecular biomarkers for environmental biomonitoring studies. We obtained the three-dimensional crystal structures of VpGSTs and MgGSTs by homologous modeling. A model to elucidate the binding interactions between the ligands and receptors was defined by molecular docking. Hydrophobic and π were the most often observed interactions between BDE-47 and the GSTs.
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identification and characterization of an intracellular cu zn superoxide dismutase iccu zn sod gene from clam Venerupis philippinarum
Fish & Shellfish Immunology, 2010Co-Authors: Huili Sun, Aiqin Chen, Xuanxuan Ning, Song Qin, Qinzhao Xue, Jianmin ZhaoAbstract:Superoxide dismutase (SOD, EC 115.11) represents one kind of enzyme involved in scavenging the high level of reactive oxygen species (ROS) into molecular oxygen and hydrogen peroxide In the present study, the intracellular Cu/Zn-SOD gene (icCu/Zn-SOD) of Venerupis philippinarum (denoted as VpSOD) was identified from haemocytes by homology cloning and RACR approaches The full-length cDNA of VpSOD consisted of 910 nucleotides with a canonical polyadenylation signal sequence AATAAA, a polyA tail, and an open-reading frame of 465 bp encoding 154 amino acids The deduced amino acid of VpSOD shared high similarity with the icCu/Zn-SODs from other species, indicating that VpSOD should be a new member of icCu/Zn-SOD family Several highly conserved motifs including Cu, Zn binding sites (H-46, H-48, H-63, H-120 for Cu binding, and H-63, H-71, H-80, D-83 for Zn binding). intracellular disulfide bond and two Cu, Zn SOD signatures were also identified in VpSOD The temporal expression of VpSOD in haemocytes after Vibrio anguillarum challenge was recorded by quantitative real-time RT-PCR. The relative expression level of VpSOD mRNA Was Lip-regulated rapidly at 6 h post-infection and reached 18-fold of the control group. After a drastic decrease at 12 h. the expression level increased again and reached 22-fold to that in the control group at 96 h post-infection. All these results indicated that VpSOD was an acute-phase protein involved in the immune responses of V philippinarum. (C) 2009 Elsevier Ltd. All rights reserved
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Cloning and characterization of an invertebrate type lysozyme from Venerupis philippinarum.
Comparative Biochemistry and Physiology B, 2010Co-Authors: Jianmin Zhao, Xuanxuan Ning, Lihua Qiu, Aiqin ChenAbstract:Lysozymes are key proteins to invertebrates in the innate immune responses against bacterial infections and providing nutrition as digestion enzymes. In the present study, an invertebrate type lysozyme (denoted as VpLYZ) was identified from Venerupis philippinarum haemocytes by cDNA library and RACE approaches. The full-length cDNA of VpLYZ consisted of 805 nucleotides with a canonical polyadenylation signal sequence AATAAA and a polyA tail, and an open-reading frame of 558 bp encoding a polypeptide of 185 amino acids with a calculated molecular mass of 20.87 kD and theoretical pl of 8.44. The high similarity of VpLYZ with other i-type lysozymes from mollusk indicated that VpLYZ should be a new member of i-type lysozyme family. Similar to most i-type lysozymes, VpLYZ possessed all conserved features critical for the fundamental structure and function of i-type lysozymes, such as three catalytic residues (Glu19, Asn72 and Ser75) and i-type specific motif CL(E/L/R/H)C(I/M)C. By semi-quantitative RT-PCR analysis, mRNA transcript of VpLYZ was found to be most abundantly expressed in the tissues of gills, hepatopancreas and haemocytes, weakly expressed in the tissues of muscle, foot and mantle. After clams were challenged by Vibrio anguillarum, the mRNA level of VpLYZ in overall haemocyte population was recorded by quantitative real-time RT-PCR. VpLYZ mRNA was down-regulated sharply from 6 h to 12 h post-infection. Then, the expression level increased to the peak at 72 h and recovered to the original level at 96 h. All these results indicated that VpLYZ was involved in the immune response against microbe infection and contributed to the clearance of bacterial pathogens. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.
Huili Sun - One of the best experts on this subject based on the ideXlab platform.
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identification and characterization of an intracellular cu zn superoxide dismutase iccu zn sod gene from clam Venerupis philippinarum
Fish & Shellfish Immunology, 2010Co-Authors: Huili Sun, Aiqin Chen, Xuanxuan Ning, Song Qin, Qinzhao Xue, Jianmin ZhaoAbstract:Superoxide dismutase (SOD, EC 115.11) represents one kind of enzyme involved in scavenging the high level of reactive oxygen species (ROS) into molecular oxygen and hydrogen peroxide In the present study, the intracellular Cu/Zn-SOD gene (icCu/Zn-SOD) of Venerupis philippinarum (denoted as VpSOD) was identified from haemocytes by homology cloning and RACR approaches The full-length cDNA of VpSOD consisted of 910 nucleotides with a canonical polyadenylation signal sequence AATAAA, a polyA tail, and an open-reading frame of 465 bp encoding 154 amino acids The deduced amino acid of VpSOD shared high similarity with the icCu/Zn-SODs from other species, indicating that VpSOD should be a new member of icCu/Zn-SOD family Several highly conserved motifs including Cu, Zn binding sites (H-46, H-48, H-63, H-120 for Cu binding, and H-63, H-71, H-80, D-83 for Zn binding). intracellular disulfide bond and two Cu, Zn SOD signatures were also identified in VpSOD The temporal expression of VpSOD in haemocytes after Vibrio anguillarum challenge was recorded by quantitative real-time RT-PCR. The relative expression level of VpSOD mRNA Was Lip-regulated rapidly at 6 h post-infection and reached 18-fold of the control group. After a drastic decrease at 12 h. the expression level increased again and reached 22-fold to that in the control group at 96 h post-infection. All these results indicated that VpSOD was an acute-phase protein involved in the immune responses of V philippinarum. (C) 2009 Elsevier Ltd. All rights reserved
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Identification and characterization of an intracellular Cu, Zn-superoxide dismutase (icCu/Zn-SOD) gene from clam Venerupis philippinarum
Fish & Shellfish Immunology, 2009Co-Authors: Huili Sun, Aiqin Chen, Xuanxuan Ning, Song Qin, Qinzhao Xue, Jianmin ZhaoAbstract:Superoxide dismutase (SOD, EC 115.11) represents one kind of enzyme involved in scavenging the high level of reactive oxygen species (ROS) into molecular oxygen and hydrogen peroxide In the present study, the intracellular Cu/Zn-SOD gene (icCu/Zn-SOD) of Venerupis philippinarum (denoted as VpSOD) was identified from haemocytes by homology cloning and RACR approaches The full-length cDNA of VpSOD consisted of 910 nucleotides with a canonical polyadenylation signal sequence AATAAA, a polyA tail, and an open-reading frame of 465 bp encoding 154 amino acids The deduced amino acid of VpSOD shared high similarity with the icCu/Zn-SODs from other species, indicating that VpSOD should be a new member of icCu/Zn-SOD family Several highly conserved motifs including Cu, Zn binding sites (H-46, H-48, H-63, H-120 for Cu binding, and H-63, H-71, H-80, D-83 for Zn binding). intracellular disulfide bond and two Cu, Zn SOD signatures were also identified in VpSOD The temporal expression of VpSOD in haemocytes after Vibrio anguillarum challenge was recorded by quantitative real-time RT-PCR. The relative expression level of VpSOD mRNA Was Lip-regulated rapidly at 6 h post-infection and reached 18-fold of the control group. After a drastic decrease at 12 h. the expression level increased again and reached 22-fold to that in the control group at 96 h post-infection. All these results indicated that VpSOD was an acute-phase protein involved in the immune responses of V philippinarum. (C) 2009 Elsevier Ltd. All rights reserved
Zhao Jianmin - One of the best experts on this subject based on the ideXlab platform.
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Identification, antibacterial activities and action mode of two macins from manila clam Venerupis philippinarum
'Elsevier BV', 2021Co-Authors: Xue Rui, Yang Dinglong, Chen Lizhu, Han Yijing, Li Fan, Zhao JianminAbstract:In this study, two macins were identified from clam Venerupis philippinarum (designated as VpMacin-1 and VpMacin-2). They showed 64.71% similarity with each other. The highest mRNA expression of VpMacin-1 and VpMacin-2 was detected in gills and hepatopancreas, respectively, in non-stimulated clams, and their expression could be induced significantly in hemocytes after Vibrio anguillarum infection. Silencing of VpMacin-1 and VpMacin-2 led to 22% and 49% mortality 6 days post infection. Escherichia coli cells were killed by recombinant protein rVpMacin-1 and rVpMacin-2 within 1000 and 400 min, respectively, at a concentration of 1.0 x MIC. Compared with rVpMacin-1, rVpMacin-2 not only showed higher broad-spectrum antimicrobial activities towards Vibrio strains, but possessed stronger abilities to inhibit the formation of bacterial biofilm. Both membrane integrity and electrochemical assay indicated that rVpMacins were capable of causing bacterial membrane permeabilization, especially for rVpMacin-2. Besides, rVpMacin-1 significantly induced both phagocytic (0.1 and 1.0 x MIC, p < 0.05) and chemotactic effects (0.1 x MIC, p < 0.01) of hemocytes, while there was no significant increase for rVpMacin-2. Overall, our results suggested that VpMacin-1 and VpMacin-2 play important roles in host defense against invasive pathogens
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Molecular characterization, expression and functional analysis of two Kazal-type serine protease inhibitors from Venerupis philippinarum
'Elsevier BV', 2017Co-Authors: Yu Qian, Yang Dinglong, Wang Qing, Wu Huifeng, Cong Ming, Li Fei, Ji Chenglong, Zhang Yingying, Zhao JianminAbstract:Kazal-type serine protease inhibitors (KSPIs) act as negative regulators in immune signaling pathway by controlling the extent of serine protease (SP) activities. In this study, the full-length cDNA of two KSPIs (designed as VpKSPI-1 and VpKSPI-2) were identified from Venerupis philippinarum by rapid amplification of cDNA ends (RACE) approaches. The open reading frame (ORF) of VpKSPI-1 and VpKSPI-2 was of 552 bp and 402 bp, encoding a polypeptide of 183 and 133 amino acids, respectively. The transcripts of VpKSPI-1 and VpKSPI-2 were ubiquitously expressed in all tissues tested with the highest expression level in hepatopancreas. After Vibrio anguillarum challenge, the relative mRNA expression of VpKSPI-1 and VpKSPI-2 in hepatopancreas was both up-regulated within 96 h. The recombinant VpKSPI-1 (rVpKSPI-1) displayed weak activities towards chymotrypsin, moderate inhibitory activity to trypsin, while rVpKSPI-2 showed significant inhibitory activities against chymotrypsin and trypsin. When the molar ratio of rVpKSPI-2 to chymotrypsin and trypsin reached 1:4 and 1:2, the protease activities could be almost entirely inhibited. All these results suggested that both VpKSPI-1 and VpKSPI-2 perhaps play a vital role in the innate immunity of V. philippinarum. (C) 2017 Elsevier Ltd. All rights reserved
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Molecular characterization, expression and antimicrobial activities of two c-type lysozymes from manila clam Venerupis philippinarum
'Elsevier BV', 2017Co-Authors: Yang Dinglong, Wang Qing, Wu Huifeng, Cong Ming, Li Fei, Ji Chenglong, Cao Ruiwen, Chen Lizhu, Liu Yongliang, Zhao JianminAbstract:Lysozymes play an important role in the innate immune responses with which mollusks respond to bacterial invasion through its lytic activity. In the present study, two c-type lysozymes (designed as VpCLYZ-1 and VpCLYZ-2, respectively) were identified and characterized from the manila clam Venerupis philippinarum. The full-length cDNA of VpCLYZ-1 and VpCLYZ-2 was of 629 and 736 bp, encoding a polypeptide of 156 and153 amino acid residues, respectively. The deduced amino acid sequences of VpCLYZs showed high similarity to other known invertebrate c-type lysozymes. Multiple alignments and phylogenetic relationship strongly suggested that VpCLYZ-1 and VpCLYZ-2 belonged to the c-type lysozyme family. Both VpCLYZ-1 and VpCLYZ-2 transcripts were constitutively expressed in a wide range of tissues with different levels. The VpCLYZ-1 transcript was dominantly expressed in hepatopancreas and hemocytes, while VpCLYZ-2 transcript was mainly expressed in the tissues of hepatopancreas and gills. Both the mRNA expression of VpCLYZ-1 and VpCLYZ-2 was significantly up-regulated at 12 h post Vibrio anguillarum challenge. The recombinant VpCLYZ-1 and VpCLYZ-2 (designed as rVpCLYZ-1 and rVpCLYZ-2) exhibited lytic activity against all tested bacteria, and rVpCLYZ-1 showed higher activities than rVpCLYZ-2 in killing Micrococcus luteus and V anguillarum. Overall, our results suggested that VpCLYZ-1 and VpCLYZ-2 belonged to the c-type lysozyme family, and played important roles in the immune responses of manila clam, especially in the elimination of pathogens. (C) 2017 Elsevier Ltd. All rights reserved
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Cloning and expression of a transcription factor activator protein-1 (AP-1) member identified from manila clam Venerupis philippinarum
'Elsevier BV', 2015Co-Authors: Wu Luning, Mu Changkao, Zhao Jianmin, Wang Chunlin, Zhang Lei, Ning Xuanxuan, Zhao, Jm Author), Chinese Acad Sci, Yantai Coastal Zone Res Inst, 17 Chunhui Rd, Laishan Dist 264003 Jmzhao@yic.ac.cnAbstract:The transcription factor activator protein-1 (AP-1) proteins are implicated to play a major role in the regulation of numerous genes involved in the function and development of the immune system, cell differentiation, proliferation, apoptosis, etc. It can bind to promoter of its target genes in a sequence-specific manner to transactivate or repress them. In this study, the full-length cDNA of an AP-1 was identified from Venerupis philippinarum (denoted as VpAP-1) by EST analysis and RACE approaches. Phylogenetic analysis indicated that VpAP-1 had higher evolutional conservation to invertebrate than vertebrate counterparts and should be a new member of the AP-1 protein family. Spatial expression analysis found that VpAP-1 transcript was most abundantly expressed in the hemocytes and hepatopancreas, weakly expressed in the tissues of the gills, mantle and muscle. After Vibrio anguillarum challenge, the expression of VpAP-1 transcript in overall hemocyte population was up-regulated in the first 6 h, and then decreased to 1.5-fold of the control group at 12 h. As time progressed, a second peak of VpAP-1 expression was detected at 24 h post-infection, which was 5-fold compared with that of the control group (P < 0.01). After that, the expression level was sharply decreased and dropped to 0.5-fold of the control at 96 h. The above results indicated that VpAP-1 was perhaps involved in the immune responses against microbe infection and might be contributed to the clearance of bacterial pathogens. (C) 2014 Published by Elsevier B.V.The transcription factor activator protein-1 (AP-1) proteins are implicated to play a major role in the regulation of numerous genes involved in the function and development of the immune system, cell differentiation, proliferation, apoptosis, etc. It can bind to promoter of its target genes in a sequence-specific manner to transactivate or repress them. In this study, the full-length cDNA of an AP-1 was identified from Venerupis philippinarum (denoted as VpAP-1) by EST analysis and RACE approaches. Phylogenetic analysis indicated that VpAP-1 had higher evolutional conservation to invertebrate than vertebrate counterparts and should be a new member of the AP-1 protein family. Spatial expression analysis found that VpAP-1 transcript was most abundantly expressed in the hemocytes and hepatopancreas, weakly expressed in the tissues of the gills, mantle and muscle. After Vibrio anguillarum challenge, the expression of VpAP-1 transcript in overall hemocyte population was up-regulated in the first 6 h, and then decreased to 1.5-fold of the control group at 12 h. As time progressed, a second peak of VpAP-1 expression was detected at 24 h post-infection, which was 5-fold compared with that of the control group (P < 0.01). After that, the expression level was sharply decreased and dropped to 0.5-fold of the control at 96 h. The above results indicated that VpAP-1 was perhaps involved in the immune responses against microbe infection and might be contributed to the clearance of bacterial pathogens. (C) 2014 Published by Elsevier B.V
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A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action
2015Co-Authors: Zhang Linbao, Yang Dinglong, Wang Qing, Yuan Zeyi, Wu Huifeng, Pei Dong, Cong Ming, Li Fei, Ji Chenglong, Zhao JianminAbstract:Antimicrobial peptides (AMPs) are important mediators of the primary host defense system against microbial invasion. In the present study, we cloned and characterized a member of the invertebrate defensin from the clam Venerupis philippinarum, designated VpDef. Amino acid sequence analysis showed that VpDef was similar to defensins from marine mollusks and ticks. In non-stimulated clams, RT-PCR and immunohistochemical analysis revealed that both VpDef mRNA and the encoding peptide were constitutively expressed in hemocytes and mantles, as well as in other major tissues. VpDef transcripts were significantly induced in hemocytes at different time intervals post Vibrio anguillarum infection. The recombinant VpDef (rVpDef) showed the highest activity against Gram-positive bacteria Micrococcus luteus and less effective to Gram-negative bacteria. In addition, incubation of rVpDef with M. luteus at 1 x and 3 x MIC could induce an obvious decrease of the membrane potential and notable changes of membrane permeability in a dose-dependent manner. Membrane integrity and bacterial viability analysis also revealed that rVpDef increased the membrane permeability of M. luteus and then resulted in cell death at 2 x and 10 x MIC. Overall, these results suggest that VpDef has an important function in host defense against invasive pathogens, probably killing microbes by inducing membrane lesions. (C) 2015 Elsevier Ltd. All rights reserved
Song Qin - One of the best experts on this subject based on the ideXlab platform.
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identification and characterization of an intracellular cu zn superoxide dismutase iccu zn sod gene from clam Venerupis philippinarum
Fish & Shellfish Immunology, 2010Co-Authors: Huili Sun, Aiqin Chen, Xuanxuan Ning, Song Qin, Qinzhao Xue, Jianmin ZhaoAbstract:Superoxide dismutase (SOD, EC 115.11) represents one kind of enzyme involved in scavenging the high level of reactive oxygen species (ROS) into molecular oxygen and hydrogen peroxide In the present study, the intracellular Cu/Zn-SOD gene (icCu/Zn-SOD) of Venerupis philippinarum (denoted as VpSOD) was identified from haemocytes by homology cloning and RACR approaches The full-length cDNA of VpSOD consisted of 910 nucleotides with a canonical polyadenylation signal sequence AATAAA, a polyA tail, and an open-reading frame of 465 bp encoding 154 amino acids The deduced amino acid of VpSOD shared high similarity with the icCu/Zn-SODs from other species, indicating that VpSOD should be a new member of icCu/Zn-SOD family Several highly conserved motifs including Cu, Zn binding sites (H-46, H-48, H-63, H-120 for Cu binding, and H-63, H-71, H-80, D-83 for Zn binding). intracellular disulfide bond and two Cu, Zn SOD signatures were also identified in VpSOD The temporal expression of VpSOD in haemocytes after Vibrio anguillarum challenge was recorded by quantitative real-time RT-PCR. The relative expression level of VpSOD mRNA Was Lip-regulated rapidly at 6 h post-infection and reached 18-fold of the control group. After a drastic decrease at 12 h. the expression level increased again and reached 22-fold to that in the control group at 96 h post-infection. All these results indicated that VpSOD was an acute-phase protein involved in the immune responses of V philippinarum. (C) 2009 Elsevier Ltd. All rights reserved
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Identification and characterization of an intracellular Cu, Zn-superoxide dismutase (icCu/Zn-SOD) gene from clam Venerupis philippinarum
Fish & Shellfish Immunology, 2009Co-Authors: Huili Sun, Aiqin Chen, Xuanxuan Ning, Song Qin, Qinzhao Xue, Jianmin ZhaoAbstract:Superoxide dismutase (SOD, EC 115.11) represents one kind of enzyme involved in scavenging the high level of reactive oxygen species (ROS) into molecular oxygen and hydrogen peroxide In the present study, the intracellular Cu/Zn-SOD gene (icCu/Zn-SOD) of Venerupis philippinarum (denoted as VpSOD) was identified from haemocytes by homology cloning and RACR approaches The full-length cDNA of VpSOD consisted of 910 nucleotides with a canonical polyadenylation signal sequence AATAAA, a polyA tail, and an open-reading frame of 465 bp encoding 154 amino acids The deduced amino acid of VpSOD shared high similarity with the icCu/Zn-SODs from other species, indicating that VpSOD should be a new member of icCu/Zn-SOD family Several highly conserved motifs including Cu, Zn binding sites (H-46, H-48, H-63, H-120 for Cu binding, and H-63, H-71, H-80, D-83 for Zn binding). intracellular disulfide bond and two Cu, Zn SOD signatures were also identified in VpSOD The temporal expression of VpSOD in haemocytes after Vibrio anguillarum challenge was recorded by quantitative real-time RT-PCR. The relative expression level of VpSOD mRNA Was Lip-regulated rapidly at 6 h post-infection and reached 18-fold of the control group. After a drastic decrease at 12 h. the expression level increased again and reached 22-fold to that in the control group at 96 h post-infection. All these results indicated that VpSOD was an acute-phase protein involved in the immune responses of V philippinarum. (C) 2009 Elsevier Ltd. All rights reserved
Qinzhao Xue - One of the best experts on this subject based on the ideXlab platform.
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identification and characterization of an intracellular cu zn superoxide dismutase iccu zn sod gene from clam Venerupis philippinarum
Fish & Shellfish Immunology, 2010Co-Authors: Huili Sun, Aiqin Chen, Xuanxuan Ning, Song Qin, Qinzhao Xue, Jianmin ZhaoAbstract:Superoxide dismutase (SOD, EC 115.11) represents one kind of enzyme involved in scavenging the high level of reactive oxygen species (ROS) into molecular oxygen and hydrogen peroxide In the present study, the intracellular Cu/Zn-SOD gene (icCu/Zn-SOD) of Venerupis philippinarum (denoted as VpSOD) was identified from haemocytes by homology cloning and RACR approaches The full-length cDNA of VpSOD consisted of 910 nucleotides with a canonical polyadenylation signal sequence AATAAA, a polyA tail, and an open-reading frame of 465 bp encoding 154 amino acids The deduced amino acid of VpSOD shared high similarity with the icCu/Zn-SODs from other species, indicating that VpSOD should be a new member of icCu/Zn-SOD family Several highly conserved motifs including Cu, Zn binding sites (H-46, H-48, H-63, H-120 for Cu binding, and H-63, H-71, H-80, D-83 for Zn binding). intracellular disulfide bond and two Cu, Zn SOD signatures were also identified in VpSOD The temporal expression of VpSOD in haemocytes after Vibrio anguillarum challenge was recorded by quantitative real-time RT-PCR. The relative expression level of VpSOD mRNA Was Lip-regulated rapidly at 6 h post-infection and reached 18-fold of the control group. After a drastic decrease at 12 h. the expression level increased again and reached 22-fold to that in the control group at 96 h post-infection. All these results indicated that VpSOD was an acute-phase protein involved in the immune responses of V philippinarum. (C) 2009 Elsevier Ltd. All rights reserved
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Identification and characterization of an intracellular Cu, Zn-superoxide dismutase (icCu/Zn-SOD) gene from clam Venerupis philippinarum
Fish & Shellfish Immunology, 2009Co-Authors: Huili Sun, Aiqin Chen, Xuanxuan Ning, Song Qin, Qinzhao Xue, Jianmin ZhaoAbstract:Superoxide dismutase (SOD, EC 115.11) represents one kind of enzyme involved in scavenging the high level of reactive oxygen species (ROS) into molecular oxygen and hydrogen peroxide In the present study, the intracellular Cu/Zn-SOD gene (icCu/Zn-SOD) of Venerupis philippinarum (denoted as VpSOD) was identified from haemocytes by homology cloning and RACR approaches The full-length cDNA of VpSOD consisted of 910 nucleotides with a canonical polyadenylation signal sequence AATAAA, a polyA tail, and an open-reading frame of 465 bp encoding 154 amino acids The deduced amino acid of VpSOD shared high similarity with the icCu/Zn-SODs from other species, indicating that VpSOD should be a new member of icCu/Zn-SOD family Several highly conserved motifs including Cu, Zn binding sites (H-46, H-48, H-63, H-120 for Cu binding, and H-63, H-71, H-80, D-83 for Zn binding). intracellular disulfide bond and two Cu, Zn SOD signatures were also identified in VpSOD The temporal expression of VpSOD in haemocytes after Vibrio anguillarum challenge was recorded by quantitative real-time RT-PCR. The relative expression level of VpSOD mRNA Was Lip-regulated rapidly at 6 h post-infection and reached 18-fold of the control group. After a drastic decrease at 12 h. the expression level increased again and reached 22-fold to that in the control group at 96 h post-infection. All these results indicated that VpSOD was an acute-phase protein involved in the immune responses of V philippinarum. (C) 2009 Elsevier Ltd. All rights reserved
