The Experts below are selected from a list of 33 Experts worldwide ranked by ideXlab platform

Laurence Bodineau - One of the best experts on this subject based on the ideXlab platform.

  • Key Brainstem Structures Activated during Hypoxic Exposure in One-day-old Mice Highlight Characteristics for Modeling Breathing Network in Premature Infants
    Frontiers in Physiology, 2016
    Co-Authors: Fanny Joubert, Camille Loiseau, Anne-sophie Perrin-terrin, Alain Frugière, Nicolas Voituron, Florence Cayetanot, Laurence Bodineau
    Abstract:

    We mapped and characterized changes in the activity of brainstem cell groups under hypoxia in one-day-old newborn mice, an animal model in which the central nervous system at birth is particularly immature. The classical biphasic respiratory response characterized by transient hyperventilation, followed by severe ventilation decline, was associated with increased c-FOS immunoreactivity in brainstem cell groups: the Nucleus of the solitary tract, Ventral Reticular Nucleus of the medulla, retrotrapezoid/parafacial region, parapyramidal group, raphe magnus Nucleus, lateral and medial parabrachial Nucleus, and dorsal subcoeruleus Nucleus. In contrast, the hypoglossal Nucleus displayed decreased c-FOS immunoreactivity. There were fewer or no activated catecholaminergic cells activated in the medulla oblongata, whereas approximately 45% of the c-FOS-positive cells in the dorsal subcoeruleus were co-labelled. Approximately 30% of the c-FOS-positive cells in the parapyramidal group were serotoninergic, whereas only a small portion were labelled for serotonin in the raphe magnus Nucleus. None of the c-FOS-positive cells in the retrotrapezoid/parafacial region were co-labelled for PHOX2B. Thus, the hypoxia-activated brainstem neuronal network of one-day-old mice is characterized by i) the activation of catecholaminergic cells of the dorsal subcoeruleus Nucleus, a structure implicated in the strong depressive pontine influence previously reported in the fetus but not in newborns, ii) the weak activation of catecholaminergic cells of the Ventral Reticular Nucleus of the medulla, an area involved in hypoxic hyperventilation, and iii) the absence of PHOX2B-positive cells activated in the retrotrapezoid/parafacial region. Based on these results, one-day-old mice could highlight characteristics for modelling the breathing network of premature infants.

  • Key Brainstem Structures Activated during Hypoxic Exposure in One-day-old Mice Highlight Characteristics for Modeling Breathing Network in Premature Infants
    Frontiers in Physiology, 2016
    Co-Authors: Fanny Joubert, Camille Loiseau, Anne-sophie Perrin-terrin, Alain Frugière, Nicolas Voituron, Florence Cayetanot, Laurence Bodineau
    Abstract:

    We mapped and characterized changes in the activity of brainstem cell groups under hypoxia in one-day-old newborn mice, an animal model in which the central nervous system at birth is particularly immature. The classical biphasic respiratory response characterized by transient hyperventilation, followed by severe ventilation decline, was associated with increased c-FOS immunoreactivity in brainstem cell groups: the Nucleus of the solitary tract, Ventral Reticular Nucleus of the medulla, retrotrapezoid/parafacial region, parapyramidal group, raphe magnus Nucleus, lateral, and medial parabrachial Nucleus, and dorsal subcoeruleus Nucleus. In contrast, the hypoglossal Nucleus displayed decreased c-FOS immunoreactivity. There were fewer or no activated catecholaminergic cells activated in the medulla oblongata, whereas ∼45% of the c-FOS-positive cells in the dorsal subcoeruleus were co-labeled. Approximately 30% of the c-FOS-positive cells in the parapyramidal group were serotoninergic, whereas only a small portion were labeled for serotonin in the raphe magnus Nucleus. None of the c-FOS-positive cells in the retrotrapezoid/parafacial region were co-labeled for PHOX2B. Thus, the hypoxia-activated brainstem neuronal network of one-day-old mice is characterized by (i) the activation of catecholaminergic cells of the dorsal subcoeruleus Nucleus, a structure implicated in the strong depressive pontine influence previously reported in the fetus but not in newborns, (ii) the weak activation of catecholaminergic cells of the Ventral Reticular Nucleus of the medulla, an area involved in hypoxic hyperventilation, and (iii) the absence of PHOX2B-positive cells activated in the retrotrapezoid/parafacial region. Based on these results, one-day-old mice could highlight characteristics for modeling the breathing network of premature infants.

Isaura Tavares - One of the best experts on this subject based on the ideXlab platform.

  • Participation of μ-opioid, GABAB, and NK1 receptors of major pain control medullary areas in pathways targeting the rat spinal cord: Implications for descending modulation of nociceptive transmission
    The Journal of comparative neurology, 2008
    Co-Authors: Marta Pinto, Marta Filipa Sousa, Deolinda Lima, Isaura Tavares
    Abstract:

    Several brain areas modulate pain transmission through direct projections to the spinal cord. The descending modulation is exerted by neurotransmitters acting both at spinally projecting neurons and at interneurons that target the projection neurons. We analyzed the expression of μ-opioid (MOR), γ-aminobutyric acid GABAB, and NK1 receptors in spinally projecting neurons of major medullary pain control areas of the rat: rostroventromedial medulla (RVM), dorsal Reticular Nucleus (DRt), Nucleus of the solitary tract, Ventral Reticular Nucleus, and lateralmost part of the caudal ventrolateral medulla. The retrograde tracer cholera toxin subunit B (CTb) was injected into the spinal dorsal horn, and medullary sections were processed by double immunocytochemistry for CTb and each receptor. The RVM contained the majority of double-labeled neurons followed by the DRt. In general, high percentages of MOR- and NK1-expressing neurons were retrogradely labeled, whereas GABAB receptors were mainly expressed in neurons that were not labeled from the cord. The results suggest that MOR and NK1 receptors play an important role in direct and indirect control of descending modulation. The co-localization of MOR and GABAB in DRt neurons also demonstrated by the present study suggests that the pronociceptive effects of this Nucleus may be controlled by local opoidergic and GABAergic inhibition of the pronociception increased during chronic pain. J. Comp. Neurol. 510:175–187, 2008. © 2008 Wiley-Liss, Inc.

  • Neuronal activation at the spinal cord and medullary pain control centers after joint stimulation: A c-fos study in acute and chronic articular inflammation
    Neuroscience, 2007
    Co-Authors: Marta Pinto, Deolinda Lima, Isaura Tavares
    Abstract:

    Chronic inflammatory pain induces short- and long-term central changes, which have been mainly studied at the spinal cord level. Supraspinal pain control centers intrinsically connected with the dorsal horn are also prone to be affected by chronic inflammatory pain. C-fos expression was used as a neuronal activation marker at spinal and supraspinal levels to i) compare acute and chronic articular inflammation, and ii) analyze the effects of brief innocuous or noxious stimulation of a chronically inflamed joint. Acute articular inflammation was induced by an inflammatory soup with prostaglandin E(2) and bradykinin, both at 10(-5) M. Chronic articular inflammation consisted of 14 days of monoarthritis. Early c-fos expression was studied 4 min after inflammatory soup injection or stimulation of the arthritic joint whereas late c-fos expression was evaluated 2 h after those stimuli. At the spinal cord, the analysis was focused on the dorsal horn (laminae I-V) and supraspinally, five major regions of the endogenous pain control system were considered: the caudal ventrolateral medulla (VLM), the dorsal Reticular Nucleus (DRt), the Ventral Reticular Nucleus (VRt), the Nucleus of the solitary tract (Sol) and the rostroventromedial medulla (RVM). Acute articular inflammation induced early and late increases in c-fos expression at the spinal level and late increases supraspinally whereas the effects of monoarthritis were more moderate and restricted to the spinal cord. When monoarthritic animals were subjected to gentle touch or bending of the joint, early increases in c-fos expression were detected supraspinally, but not at the spinal level. In this region, noxious mechanical stimulation induced late increases in non-inflamed animals and both early and late increases in monoarthritic rats. Supraspinally, noxious stimulation induced only late increases in c-fos expression. The present results show complex differences in the patterns of c-fos expression between the spinal cord and medullary areas of the pain control system during articular inflammation, which indicate that the somatosensory system is differentially affected by the installation of chronic pain.

  • Noxious‐evoked c‐fos expression in brainstem neurons immunoreactive for GABAB, µ‐opioid and NK‐1 receptors
    The European journal of neuroscience, 2003
    Co-Authors: Marta Pinto, Deolinda Lima, José Manuel Castro-lopes, Isaura Tavares
    Abstract:

    Modulation of nociceptive transmission at the brainstem involves several neurochemical systems. The precise location and specific characteristics of nociceptive neurons activated in each system was never reported. In thisstudy, the presence of GABA B , μ-opioid, and neurokinin-1 (NK-1) receptors in brainstem nociceptive neurons was investigated by double-immunocytochemical detection of each receptor and noxious-evoked induction of the c-fos proto-oncogene. Noxious cutaneous mechanical stimulation significantly increased the proportions of neurons double-labelled for Fos and GABA B receptors in several brainstem regions, namely, the Reticular formation of the caudal ventrolateral medulla (VLMlat and VLMrf), lateral Reticular Nucleus, spinal trigeminal Nucleus, pars caudalis (Sp 5 C), Nucleus of the solitary tract, dorsal Reticular Nucleus, Ventral Reticular Nucleus, raphe obscurus Nucleus and dorsal parabrachial Nucleus (DPB). For μ-opioid receptors, the proportions of double-labelled neurons in noxious-stimulated animals were higher than in controls only in the VLMlat, VLMrf, Sp 5 C, DPB and A 5 noradrenergic cell group. As for the NK-1 receptor, no significant differences were found between control and stimulated animals. According to these results, neurons expressing GABA B , μ-opioid and NK-1 receptors at several pain control centres of the brainstem are differentially involved in processing nociceptive mechanical input. The data provide the definition of new supraspinal targets for selective modulation of nociceptive neurons in order to define better strategies of pain control.

Fanny Joubert - One of the best experts on this subject based on the ideXlab platform.

  • Key Brainstem Structures Activated during Hypoxic Exposure in One-day-old Mice Highlight Characteristics for Modeling Breathing Network in Premature Infants
    Frontiers in Physiology, 2016
    Co-Authors: Fanny Joubert, Camille Loiseau, Anne-sophie Perrin-terrin, Alain Frugière, Nicolas Voituron, Florence Cayetanot, Laurence Bodineau
    Abstract:

    We mapped and characterized changes in the activity of brainstem cell groups under hypoxia in one-day-old newborn mice, an animal model in which the central nervous system at birth is particularly immature. The classical biphasic respiratory response characterized by transient hyperventilation, followed by severe ventilation decline, was associated with increased c-FOS immunoreactivity in brainstem cell groups: the Nucleus of the solitary tract, Ventral Reticular Nucleus of the medulla, retrotrapezoid/parafacial region, parapyramidal group, raphe magnus Nucleus, lateral and medial parabrachial Nucleus, and dorsal subcoeruleus Nucleus. In contrast, the hypoglossal Nucleus displayed decreased c-FOS immunoreactivity. There were fewer or no activated catecholaminergic cells activated in the medulla oblongata, whereas approximately 45% of the c-FOS-positive cells in the dorsal subcoeruleus were co-labelled. Approximately 30% of the c-FOS-positive cells in the parapyramidal group were serotoninergic, whereas only a small portion were labelled for serotonin in the raphe magnus Nucleus. None of the c-FOS-positive cells in the retrotrapezoid/parafacial region were co-labelled for PHOX2B. Thus, the hypoxia-activated brainstem neuronal network of one-day-old mice is characterized by i) the activation of catecholaminergic cells of the dorsal subcoeruleus Nucleus, a structure implicated in the strong depressive pontine influence previously reported in the fetus but not in newborns, ii) the weak activation of catecholaminergic cells of the Ventral Reticular Nucleus of the medulla, an area involved in hypoxic hyperventilation, and iii) the absence of PHOX2B-positive cells activated in the retrotrapezoid/parafacial region. Based on these results, one-day-old mice could highlight characteristics for modelling the breathing network of premature infants.

  • Key Brainstem Structures Activated during Hypoxic Exposure in One-day-old Mice Highlight Characteristics for Modeling Breathing Network in Premature Infants
    Frontiers in Physiology, 2016
    Co-Authors: Fanny Joubert, Camille Loiseau, Anne-sophie Perrin-terrin, Alain Frugière, Nicolas Voituron, Florence Cayetanot, Laurence Bodineau
    Abstract:

    We mapped and characterized changes in the activity of brainstem cell groups under hypoxia in one-day-old newborn mice, an animal model in which the central nervous system at birth is particularly immature. The classical biphasic respiratory response characterized by transient hyperventilation, followed by severe ventilation decline, was associated with increased c-FOS immunoreactivity in brainstem cell groups: the Nucleus of the solitary tract, Ventral Reticular Nucleus of the medulla, retrotrapezoid/parafacial region, parapyramidal group, raphe magnus Nucleus, lateral, and medial parabrachial Nucleus, and dorsal subcoeruleus Nucleus. In contrast, the hypoglossal Nucleus displayed decreased c-FOS immunoreactivity. There were fewer or no activated catecholaminergic cells activated in the medulla oblongata, whereas ∼45% of the c-FOS-positive cells in the dorsal subcoeruleus were co-labeled. Approximately 30% of the c-FOS-positive cells in the parapyramidal group were serotoninergic, whereas only a small portion were labeled for serotonin in the raphe magnus Nucleus. None of the c-FOS-positive cells in the retrotrapezoid/parafacial region were co-labeled for PHOX2B. Thus, the hypoxia-activated brainstem neuronal network of one-day-old mice is characterized by (i) the activation of catecholaminergic cells of the dorsal subcoeruleus Nucleus, a structure implicated in the strong depressive pontine influence previously reported in the fetus but not in newborns, (ii) the weak activation of catecholaminergic cells of the Ventral Reticular Nucleus of the medulla, an area involved in hypoxic hyperventilation, and (iii) the absence of PHOX2B-positive cells activated in the retrotrapezoid/parafacial region. Based on these results, one-day-old mice could highlight characteristics for modeling the breathing network of premature infants.

Marta Pinto - One of the best experts on this subject based on the ideXlab platform.

  • Participation of μ-opioid, GABAB, and NK1 receptors of major pain control medullary areas in pathways targeting the rat spinal cord: Implications for descending modulation of nociceptive transmission
    The Journal of comparative neurology, 2008
    Co-Authors: Marta Pinto, Marta Filipa Sousa, Deolinda Lima, Isaura Tavares
    Abstract:

    Several brain areas modulate pain transmission through direct projections to the spinal cord. The descending modulation is exerted by neurotransmitters acting both at spinally projecting neurons and at interneurons that target the projection neurons. We analyzed the expression of μ-opioid (MOR), γ-aminobutyric acid GABAB, and NK1 receptors in spinally projecting neurons of major medullary pain control areas of the rat: rostroventromedial medulla (RVM), dorsal Reticular Nucleus (DRt), Nucleus of the solitary tract, Ventral Reticular Nucleus, and lateralmost part of the caudal ventrolateral medulla. The retrograde tracer cholera toxin subunit B (CTb) was injected into the spinal dorsal horn, and medullary sections were processed by double immunocytochemistry for CTb and each receptor. The RVM contained the majority of double-labeled neurons followed by the DRt. In general, high percentages of MOR- and NK1-expressing neurons were retrogradely labeled, whereas GABAB receptors were mainly expressed in neurons that were not labeled from the cord. The results suggest that MOR and NK1 receptors play an important role in direct and indirect control of descending modulation. The co-localization of MOR and GABAB in DRt neurons also demonstrated by the present study suggests that the pronociceptive effects of this Nucleus may be controlled by local opoidergic and GABAergic inhibition of the pronociception increased during chronic pain. J. Comp. Neurol. 510:175–187, 2008. © 2008 Wiley-Liss, Inc.

  • Neuronal activation at the spinal cord and medullary pain control centers after joint stimulation: A c-fos study in acute and chronic articular inflammation
    Neuroscience, 2007
    Co-Authors: Marta Pinto, Deolinda Lima, Isaura Tavares
    Abstract:

    Chronic inflammatory pain induces short- and long-term central changes, which have been mainly studied at the spinal cord level. Supraspinal pain control centers intrinsically connected with the dorsal horn are also prone to be affected by chronic inflammatory pain. C-fos expression was used as a neuronal activation marker at spinal and supraspinal levels to i) compare acute and chronic articular inflammation, and ii) analyze the effects of brief innocuous or noxious stimulation of a chronically inflamed joint. Acute articular inflammation was induced by an inflammatory soup with prostaglandin E(2) and bradykinin, both at 10(-5) M. Chronic articular inflammation consisted of 14 days of monoarthritis. Early c-fos expression was studied 4 min after inflammatory soup injection or stimulation of the arthritic joint whereas late c-fos expression was evaluated 2 h after those stimuli. At the spinal cord, the analysis was focused on the dorsal horn (laminae I-V) and supraspinally, five major regions of the endogenous pain control system were considered: the caudal ventrolateral medulla (VLM), the dorsal Reticular Nucleus (DRt), the Ventral Reticular Nucleus (VRt), the Nucleus of the solitary tract (Sol) and the rostroventromedial medulla (RVM). Acute articular inflammation induced early and late increases in c-fos expression at the spinal level and late increases supraspinally whereas the effects of monoarthritis were more moderate and restricted to the spinal cord. When monoarthritic animals were subjected to gentle touch or bending of the joint, early increases in c-fos expression were detected supraspinally, but not at the spinal level. In this region, noxious mechanical stimulation induced late increases in non-inflamed animals and both early and late increases in monoarthritic rats. Supraspinally, noxious stimulation induced only late increases in c-fos expression. The present results show complex differences in the patterns of c-fos expression between the spinal cord and medullary areas of the pain control system during articular inflammation, which indicate that the somatosensory system is differentially affected by the installation of chronic pain.

  • Noxious‐evoked c‐fos expression in brainstem neurons immunoreactive for GABAB, µ‐opioid and NK‐1 receptors
    The European journal of neuroscience, 2003
    Co-Authors: Marta Pinto, Deolinda Lima, José Manuel Castro-lopes, Isaura Tavares
    Abstract:

    Modulation of nociceptive transmission at the brainstem involves several neurochemical systems. The precise location and specific characteristics of nociceptive neurons activated in each system was never reported. In thisstudy, the presence of GABA B , μ-opioid, and neurokinin-1 (NK-1) receptors in brainstem nociceptive neurons was investigated by double-immunocytochemical detection of each receptor and noxious-evoked induction of the c-fos proto-oncogene. Noxious cutaneous mechanical stimulation significantly increased the proportions of neurons double-labelled for Fos and GABA B receptors in several brainstem regions, namely, the Reticular formation of the caudal ventrolateral medulla (VLMlat and VLMrf), lateral Reticular Nucleus, spinal trigeminal Nucleus, pars caudalis (Sp 5 C), Nucleus of the solitary tract, dorsal Reticular Nucleus, Ventral Reticular Nucleus, raphe obscurus Nucleus and dorsal parabrachial Nucleus (DPB). For μ-opioid receptors, the proportions of double-labelled neurons in noxious-stimulated animals were higher than in controls only in the VLMlat, VLMrf, Sp 5 C, DPB and A 5 noradrenergic cell group. As for the NK-1 receptor, no significant differences were found between control and stimulated animals. According to these results, neurons expressing GABA B , μ-opioid and NK-1 receptors at several pain control centres of the brainstem are differentially involved in processing nociceptive mechanical input. The data provide the definition of new supraspinal targets for selective modulation of nociceptive neurons in order to define better strategies of pain control.

Camille Loiseau - One of the best experts on this subject based on the ideXlab platform.

  • Key Brainstem Structures Activated during Hypoxic Exposure in One-day-old Mice Highlight Characteristics for Modeling Breathing Network in Premature Infants
    Frontiers in Physiology, 2016
    Co-Authors: Fanny Joubert, Camille Loiseau, Anne-sophie Perrin-terrin, Alain Frugière, Nicolas Voituron, Florence Cayetanot, Laurence Bodineau
    Abstract:

    We mapped and characterized changes in the activity of brainstem cell groups under hypoxia in one-day-old newborn mice, an animal model in which the central nervous system at birth is particularly immature. The classical biphasic respiratory response characterized by transient hyperventilation, followed by severe ventilation decline, was associated with increased c-FOS immunoreactivity in brainstem cell groups: the Nucleus of the solitary tract, Ventral Reticular Nucleus of the medulla, retrotrapezoid/parafacial region, parapyramidal group, raphe magnus Nucleus, lateral and medial parabrachial Nucleus, and dorsal subcoeruleus Nucleus. In contrast, the hypoglossal Nucleus displayed decreased c-FOS immunoreactivity. There were fewer or no activated catecholaminergic cells activated in the medulla oblongata, whereas approximately 45% of the c-FOS-positive cells in the dorsal subcoeruleus were co-labelled. Approximately 30% of the c-FOS-positive cells in the parapyramidal group were serotoninergic, whereas only a small portion were labelled for serotonin in the raphe magnus Nucleus. None of the c-FOS-positive cells in the retrotrapezoid/parafacial region were co-labelled for PHOX2B. Thus, the hypoxia-activated brainstem neuronal network of one-day-old mice is characterized by i) the activation of catecholaminergic cells of the dorsal subcoeruleus Nucleus, a structure implicated in the strong depressive pontine influence previously reported in the fetus but not in newborns, ii) the weak activation of catecholaminergic cells of the Ventral Reticular Nucleus of the medulla, an area involved in hypoxic hyperventilation, and iii) the absence of PHOX2B-positive cells activated in the retrotrapezoid/parafacial region. Based on these results, one-day-old mice could highlight characteristics for modelling the breathing network of premature infants.

  • Key Brainstem Structures Activated during Hypoxic Exposure in One-day-old Mice Highlight Characteristics for Modeling Breathing Network in Premature Infants
    Frontiers in Physiology, 2016
    Co-Authors: Fanny Joubert, Camille Loiseau, Anne-sophie Perrin-terrin, Alain Frugière, Nicolas Voituron, Florence Cayetanot, Laurence Bodineau
    Abstract:

    We mapped and characterized changes in the activity of brainstem cell groups under hypoxia in one-day-old newborn mice, an animal model in which the central nervous system at birth is particularly immature. The classical biphasic respiratory response characterized by transient hyperventilation, followed by severe ventilation decline, was associated with increased c-FOS immunoreactivity in brainstem cell groups: the Nucleus of the solitary tract, Ventral Reticular Nucleus of the medulla, retrotrapezoid/parafacial region, parapyramidal group, raphe magnus Nucleus, lateral, and medial parabrachial Nucleus, and dorsal subcoeruleus Nucleus. In contrast, the hypoglossal Nucleus displayed decreased c-FOS immunoreactivity. There were fewer or no activated catecholaminergic cells activated in the medulla oblongata, whereas ∼45% of the c-FOS-positive cells in the dorsal subcoeruleus were co-labeled. Approximately 30% of the c-FOS-positive cells in the parapyramidal group were serotoninergic, whereas only a small portion were labeled for serotonin in the raphe magnus Nucleus. None of the c-FOS-positive cells in the retrotrapezoid/parafacial region were co-labeled for PHOX2B. Thus, the hypoxia-activated brainstem neuronal network of one-day-old mice is characterized by (i) the activation of catecholaminergic cells of the dorsal subcoeruleus Nucleus, a structure implicated in the strong depressive pontine influence previously reported in the fetus but not in newborns, (ii) the weak activation of catecholaminergic cells of the Ventral Reticular Nucleus of the medulla, an area involved in hypoxic hyperventilation, and (iii) the absence of PHOX2B-positive cells activated in the retrotrapezoid/parafacial region. Based on these results, one-day-old mice could highlight characteristics for modeling the breathing network of premature infants.