The Experts below are selected from a list of 237 Experts worldwide ranked by ideXlab platform
Weijie Zhao - One of the best experts on this subject based on the ideXlab platform.
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total steroidal alkaloids from Veratrum patulum l inhibit platelet aggregation thrombi formation and decrease bleeding time in rats
Journal of Ethnopharmacology, 2012Co-Authors: Qiling Song, Shisheng Wang, Weijie ZhaoAbstract:Abstract Ethnopharmacological relevance Veratrum patulum L. (Liliaceae) is used as one source of the Chinese traditional drug “Lilu” which has been used in the treatment of aphasia arising from apoplexy, wind type dysentery, headache, etc . for thousands of years. Aim of the study To evaluate the antithrombotic effect of Veratrum patulum L. (Liliaceae) total steroidal alkaloids (VpA) and explore the potential mechanisms. Materials and methods The antithrombotic effect of VpA was evaluated in two experimental thrombosis models in rat. The potential antithrombotic mechanisms of VpA were explored by determining the coagulation parameters and platelet aggregation induced by various agonists. Cutting-tail rat model was used to evaluate the influence of VpA on bleeding time. Results VpA significantly inhibit the formation of arterial and venous thrombosis and showed different inhibitory effect on the platelet aggregation induced by various agonists with the following potency order: collagen > ADP > thrombin. VpA showed no influence on the coagulation parameters in rat. But VpA decreased the cutting-tail bleeding time in rat. Conclusion VpA possess antithrombotic effect on venous and arterial thrombosis. The antithrombotic effect of VpA is due to its inhibition to platelet aggregation, especially induced by collagen.
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two new steroidal alkaloids from Veratrum nigrum var ussuriense
Chinese Chemical Letters, 2010Co-Authors: Xue Feng Zhou, Weijie Zhao, Zhi Gang Gao, Xu Ran Han, Shisheng WangAbstract:Abstract Two new steroidal alkaloids, veraussines A ( 1 ) and B ( 2 ) were isolated from the roots and rhizomes of Veratrum nigrum var. ussuriense . Their structures were determined as N-(ethoxycarbonyl)-1 α ,2 β ,3 α ,15 α -tetrahydroxy-5 β -jervanin-12-en-11-one ( 1 ) and N -(methoxycarbonyl)-1 α ,2 β ,3 α ,15 α -tetrahydroxy-5 β -jervanin-12-en-11-one ( 2 ) by spectroscopic analysis.
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antithrombotic effects in rats of germidine an alkaloid from Veratrum nigram l var ussuriense nakai
2008Co-Authors: Weijie Zhao, Shisheng Wang, Weifeng Deng, Ping PanAbstract:Germidine is one of ester type steroidal alkaloids extracted from Veratrum Nigrum L. var. ussuriense Nakai ("Wusuli Lilu" in Chinese), a herbal medicine that has long been used as an anti-hypertensive remedy in Chinese traditional medicine. Although the antithrombotic effects of the Veratrum nigrum alkaloids (VnA) have been reported , the effective chemical constituent of VnA is still unclear. In view of this fact, we investigated the inhibitory activity of germidine on thrombosis in rats. In rat carotid artery thrombosis model, germidine (iv 1.25-20.0 µg·kg -1 ) significantly and dose-dependently prolonged the occlusion time (OT) of carotid artery injured by electrical stimulation. In rat inferior vena thrombosis model, germidine (iv 2.5-20.0 µg·kg -1 ) decreased the thrombus dry weight in a significant and dose-dependent manner. LD50(iv) in mice of germidine was found to be 6.6619 mg·kg -1 , a value much greater than that of VnA. We conclude that germidine has powerful inhibitory effects against both arterial and venous thrombosis in rats and plays an important role in the antithrombotic effects of VnA.
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protection of Veratrum nigrum l var ussuriense nakai alkaloids against ischemia reperfusion injury of the rat liver
World Journal of Gastroenterology, 2007Co-Authors: Zhenzhen Wang, Weijie Zhao, Xuesong Zhang, Xiaofeng Tian, Yuzhu Wang, Feng Zhang, Jinchan Yuan, Guozhu Han, Kexin Liu, Jihong YaoAbstract:Protection of Veratrum nigrum L. var. ussuriense Nakai alkaloids against ischemia-reperfusion injury of the rat liver
Jin-hui Wang - One of the best experts on this subject based on the ideXlab platform.
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alkaloid profiling of crude and processed Veratrum nigrum l through simultaneous determination of ten steroidal alkaloids by hplc elsd
Journal of Pharmaceutical and Biomedical Analysis, 2008Co-Authors: Yue Cong, Yu-bo Zhou, Jing Chen, Jin-hui Wang, Yimei ZengAbstract:Veratrum nigrum L., a traditional Chinese herb, has been used for treatment of hypertension, blood-stroke, excessive phlegm, epilepsy, etc. Steroidal alkaloids were well-known as both bioactive and toxic constituents of Veratrum species, the toxicity of which the traditional processing procedure can reduce. To reveal the mechanism of processing V. nigrum L., a high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) method was developed for the simultaneous determination of ten steroidal alkaloids in crude and processed V. nigrum L., comparison with ultrasound extract of crude V. nigrum L. With a Venusil XBP-C(18) analytical column, the analytes were separated efficiently using the mobile phase consisted of (A) 0.03% aqueous triethylamine (TEA) and (B) acetonitrile in a gradient program. The parameters for ELSD were set: S.C. (Spray Chamber)=35 degrees C, D.T. (Drift Tube)=70 degrees C, GAS=50 psi. All calibration curves showed good linear regression (gamma>or=0.9990) within the tested range. Additionally, reproducibility for the quantification of ten alkaloids in V. nigrum L. with intra- and inter-day variations of less than 5.0% was observed. The obtained alkaloid profiles performed by this newly established method, provided valuable information for the differentiation of crude and processed V. nigrum L. and for the explanation of the different toxicity.
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Steroidal alkaloids from Veratrum japonicum with genotoxicity on brain cell DNA of the cerebellum and cerebral cortex in mice.
Planta medica, 2007Co-Authors: Yue Cong, Lei Guo, Jingyu Yang, Yu-bo Zhou, Jing Chen, Jin-hui WangAbstract:A new steroidal alkaloid ( 1), together with three known steroidal alkaloids ( 2 - 4), has been isolated from the roots and rhizomes of Veratrum japonicum (Baker) Loes. f.., their structures were established as neogermine ( 1), germine ( 2), germerine ( 3), and neogermbudine ( 4) by means of physicochemical properties and spectroscopic analysis. Compounds 1 - 4 exhibited genotoxicity on brain cells in mice by using single-cell gel electrophoresis (Comet assay).
Jihong Yao - One of the best experts on this subject based on the ideXlab platform.
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protection of Veratrum nigrum l var ussuriense nakai alkaloids against ischemia reperfusion injury of the rat liver
World Journal of Gastroenterology, 2007Co-Authors: Zhenzhen Wang, Weijie Zhao, Xuesong Zhang, Xiaofeng Tian, Yuzhu Wang, Feng Zhang, Jinchan Yuan, Guozhu Han, Kexin Liu, Jihong YaoAbstract:Protection of Veratrum nigrum L. var. ussuriense Nakai alkaloids against ischemia-reperfusion injury of the rat liver
Atta-ur-rahman - One of the best experts on this subject based on the ideXlab platform.
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Steroidal alkaloids from Veratrum album
Phytochemistry, 1996Co-Authors: Atta-ur-rahman, Rahat Azhar Ali, M. Iqbal Choudhary, Bilge Sener, M. Ashraf, Songul TurkozAbstract:Two new steroidal alkaloids, veramanine and neojerminalanine, have been isolated from the rhizomes of Veratrum album, along with the known glycoalkaloid, pseudojervine, whose NMR spectral data are reported for the first time. The structures of 1 and 2 were elucidated on the basis of extensive spectroscopic investigations.
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Alkaloids from Veratrum album
Phytochemistry, 1991Co-Authors: Atta-ur-rahman, Rahat Azhar Ali, Tahira Parveen, M. Iqbal Choudhary, Bilge Sener, Sungul TurkozAbstract:Abstract Leaves of Veratrum album of Turkish origin have yielded two new steroidal alkaloids, O -acetyljervine and methyljervine- N -3′-propanoate along with the known alkaloid jervine. The structures of these new bases were established on the basis of 2D NMR and other spectroscopic techniques.
Veselin Christov - One of the best experts on this subject based on the ideXlab platform.
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alkaloids derived from genus Veratrum and peganum of mongolian origin as multidrug resistance inhibitors of cancer cells
Fitoterapia, 2011Co-Authors: Antoaneta Ivanova, Veselin Christov, Julianna Serly, Bistra A Stamboliyska, Joseph MolnarAbstract:Alkaloids comprise one of the largest groups of plant secondary metabolites including vinca alkaloids. The ability of six alkaloids from Veratrum lobelianum, one from Veratrum nigrum and three from Peganum nigellastrum to modify transport activity of MDR1 was studied. Flow-cytometry in a multidrug-resistant human MDR1-gene-transfected mouse lymphoma cells (L5178Y) was applied. The inhibition of multidrug resistance was investigated by measuring the accumulation of rhodamine-123 in cancer cells. Veralosinine and veranigrine were the most effective resistance modifiers. In a checkerboard method veralosinine and veranigrine enhanced the antiproliferative effects of doxorubicin on MDR cells in combination. The structure-activity relationships were discussed.
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steroidal alkaloids of Veratrum lobelianum bernh and Veratrum nigrum l
Zeitschrift für Naturforschung C, 2010Co-Authors: Veselin Christov, Bozhanka Mikhova, Antoaneta Ivanova, Julianna Serly, Joseph Molnar, D Selenge, Amgalan Solongo, Nadezhda Kostova, Yadamsuren Gereltod, Dimitar DimitrovAbstract:Twelve steroidal alkaloids were isolated from four populations of Veratrum lobelianum Bernh. and Veratrum nigrum L. Full NMR data for veralosinine (1), and extensive 1H NMR data for veralosine (3) and teinemine (5) are presented here for the first time. (+/-)-15-O-(2-Methylbutyroyl)germine (10) is undescribed up to now. The antiproliferative activities of veranigrine, veralosinine, and neogermitrine have shown that they are a perspective for further studies.
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(-)-Veranigrine, a new steroidal alkaloid from Veratrum nigrum L.
Fitoterapia, 2008Co-Authors: Veselin Christov, Bozhanka Mikhova, D SelengeAbstract:A new steroidal alkaloid, (-)-veranigrine (1) was isolated from the roots and rhizomes of Veratrum nigrum L. By means of spectroscopic methods its structure was established as (20S, 25S)-iminocholesta-5,22(N)-diene-1beta,3beta-diol.
