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Hermann Ehrlich - One of the best experts on this subject based on the ideXlab platform.
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Enigmatic Structural Protein Spongin
Marine Biological Materials of Invertebrate Origin, 2019Co-Authors: Hermann EhrlichAbstract:In the orders Dendroceratida, Verongida and Dictyoceratida, the characteristic for most Demospongiae siliceous skeleton is replaced by proteinaceous, fibrous like spongin skeleton. These spongin fibers can be anastomosed in order to create a network which provides support for skeleton of the sponge’s cell tissues. This network represents sets of diverse unconnected, mostly dendritic three dimensional structures. From chemical point of view, spongin remains to be an enigmatic proteinaceous biomaterial than contains halogenated residues and cannot be sequences till now. Consequently it was defined previously as pseudoceratin, euceratin, horny or sclerotized protein, iodospongin, silk-, or gelatin-like protein, etc. State-of the art concerning diversity, biological functions, and material features of spongin are described and discussed in this chapter.
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isolation and identification of chitin from heavy mineralized skeleton of suberea clavata Verongida demospongiae porifera marine demosponge
International Journal of Biological Macromolecules, 2017Co-Authors: Hermann Ehrlich, Vasilii V Bazhenov, Cecile Debitus, Nicole J De Voogd, Roberta Galli, Mikhail V Tsurkan, Marcin Wysokowski, Heike Meissner, Esra BulutAbstract:Since the discovery of chitin in skeletal structures of sponges (Porifera) in 2007, studies on search of novel species which possess this structural aminopolysaccharide continue up today. The most potential source of chitin is suggested to be localized in the four families of sponges related to the order Verongida (Demospongiae) which nevertheless require further clarification. Here, we report for the first time the isolation and identification of α-chitin from the Suberea clavata demosponge (Aplysinidae: Verongida). Raman spectroscopy, Calcofluor White staining, chitinase test and ESI-MS techniques were used to identify chitin. We suggest that the presence of chitin within fibrous skeletons of diverse species of Verongida order, and, especially in all species of the Aplysinidae family, may be useful for the identification of novel, previously unidentified marine demosponges.
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Brominated Skeletal Components of the Marine Demosponges, Aplysina cavernicola and Ianthella basta: Analytical and Biochemical Investigations
Marine Drugs, 2013Co-Authors: Kurt Kunze, Hermann Ehrlich, Susanne Ueberlein, Eike Brunner, Hendrik Niemann, Renate Schulze, Peter ProkschAbstract:Demosponges possess a skeleton made of a composite material with various organic constituents and/or siliceous spicules. Chitin is an integral part of the skeleton of different sponges of the order Verongida. Moreover, sponges of the order Verongida, such as Aplysina cavernicola or Ianthella basta, are well-known for the biosynthesis of brominated tyrosine derivates, characteristic bioactive natural products. It has been unknown so far whether these compounds are exclusively present in the cellular matrix or whether they may also be incorporated into the chitin-based skeletons. In the present study, we therefore examined the skeletons of A. cavernicola and I. basta with respect to the presence of bromotyrosine metabolites. The chitin-based-skeletons isolated from these sponges indeed contain significant amounts of brominated compounds, which are not easily extractable from the skeletons by common solvents, such as MeOH, as shown by HPLC analyses in combination with NMR and IR spectroscopic measurements. Quantitative potentiometric analyses confirm that the skeleton-associated bromine mainly withstands the MeOH-based extraction. This observation suggests that the respective, but yet unidentified, brominated compounds are strongly bound to the sponge skeletons, possibly by covalent bonding. Moreover, gene fragments of halogenases suggested to be responsible for the incorporation of bromine into organic molecules could be amplified from DNA isolated from sponge samples enriched for sponge-associated bacteria
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Chitin-based renewable materials from marine sponges for uranium adsorption
Carbohydrate Polymers, 2012Co-Authors: Dorothea Schleuter, Hermann Ehrlich, Alix Günther, Silvia Paasch, Zoran Kljajić, Thomas Hanke, Gert Bernhard, Eike BrunnerAbstract:Abstract Marine sponges of the order Verongida form three-dimensional networks of fibrous chitin, which can easily be extracted. In the hydrated state, these networks are flexible, mechanically stable and can be cut or pressed into any desired form. Here, we show for the first time that chitin-based networks of sponge origin are useful for effective uranium adsorption. They adsorb uranium from solution with a higher adsorption capacity than many other chitinous sorbents. Up to 288 mg/g could be achieved. Solid-state NMR, infrared, and Raman spectroscopy indicated that the uranyl is bound to the chitin by weak interactions. 90 % of the uranyl could be desorbed using diluted hydrochloric acid. Uranium adsorption and desorption did not result in any destruction of the chitin-based material.
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integrative taxonomy and molecular phylogeny of genus aplysina demospongiae Verongida from mexican pacific
PLOS ONE, 2012Co-Authors: Jose Antonio Cruzbarraza, Axayacatl Rochaolivares, Jose Luis Carballo, Hermann EhrlichAbstract:Integrative taxonomy provides a major approximation to species delimitation based on integration of different perspectives (e.g. morphology, biochemistry and DNA sequences). The aim of this study was to assess the relationships and boundaries among Eastern Pacific Aplysina species using morphological, biochemical and molecular data. For this, a collection of sponges of the genus Aplysina from the Mexican Pacific was studied on the basis of their morphological, chemical (chitin composition), and molecular markers (mitochondrial COI and nuclear ribosomal rDNA: ITS1-5.8-ITS2). Three morphological species were identified, two of which are new to science. A. clathrata sp. nov. is a yellow to yellow-reddish or -brownish sponge, characterized by external clathrate-like morphology; A. revillagigedi sp. nov. is a lemon yellow to green, cushion-shaped sometimes lobate sponge, characterized by conspicuous oscules, which are slightly elevated and usually linearly distributed on rims; and A. gerardogreeni a known species distributed along the Mexican Pacific coast. Chitin was identified as the main structural component within skeletons of the three species using FTIR, confirming that it is shared among Verongida sponges. Morphological differences were confirmed by DNA sequences from nuclear ITS1-5.8-ITS2. Mitochondrial COI sequences showed extremely low but diagnostic variability for Aplysina revillagigedi sp. nov., thus our results corroborate that COI has limited power for DNA-barcoding of sponges and should be complemented with other markers (e.g. rDNA). Phylogenetic analyses of Aplysina sequences from the Eastern Pacific and Caribbean, resolved two allopatric and reciprocally monophyletic groups for each region. Eastern Pacific species were grouped in general accordance with the taxonomic hypothesis based on morphological characters. An identification key of Eastern Pacific Aplysina species is presented. Our results constitute one of the first approximations to integrative taxonomy, phylogeny and evolutionary biogeography of Eastern Pacific marine sponges; an approach that will significantly contribute to our better understanding of their diversity and evolutionary history.
Maurizio Pansini - One of the best experts on this subject based on the ideXlab platform.
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chemistry of Verongida sponges 10 secondary metabolite composition of the caribbean sponge verongula gigantea
Journal of Natural Products, 2000Co-Authors: Patrizia Ciminiello, Carmela Dellaversano, Ernesto Fattorusso, Silvana Magno, Maurizio PansiniAbstract:A detailed analysis of the secondary metabolites of the Caribbean sponge Verongula gigantea has been performed. A number of bromotyrosine derivatives, 1, 2, and 6−17, were identified, one of which (17) is a novel compound. Its structure was determined on the basis of spectroscopic evidence. Additionally, aureol (18) and 5,6-dibromo-N,N-dimethyltryptamine (19) were isolated from one of the five analyzed specimens.
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chemistry of Verongida sponges 9 1 secondary metabolite composition of the caribbean sponge aplysina cauliformis
Journal of Natural Products, 1999Co-Authors: Patrizia Ciminiello, Carmela Dellaversano, Ernesto Fattorusso, Silvana Magno, Maurizio PansiniAbstract:A detailed analysis of the secondary metabolites of the sponge Aplysina cauliformis has been performed. Eight compounds were identified, two of which (13 and 14) are new bromotyrosine derivatives whose structures were determinated from spectroscopic evidence, including 2D NMR. The new compounds were analyzed for cytotoxic activity, and compound 14 was shown to inhibit mammalian protein synthesis and cell proliferation.
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chemistry of Verongida sponges viii1 bromocompounds from the mediterranean sponges aplysina aerophoba and aplysina cavernicola
Tetrahedron, 1997Co-Authors: Patrizia Ciminiello, Ernesto Fattorusso, Silvana Magno, Martino Forino, Maurizio PansiniAbstract:Abstract A detailed analysis of the secondary metabolites of Aplysina aerophoba and Aplysina cavernicola has been performed. A number of bromotyrosine derivatives have been identified of which two, 14 and 15, both isolated from A. cavernicola, are novel compounds. Their structures have been assigned on the basis of spectroscopic and chemical data. The obtained results indicate a remarkable difference in the secondary metabolism of the two sponges, which eventually confirms the separation of the two Mediterranean Aplysina species, in perfect agreement with Vacelet's opinion.
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chemistry of Verongida sponges vii bromocompounds from the caribbean sponge aplysina archeri
Tetrahedron, 1996Co-Authors: Patrizia Ciminiello, Carmela Dellaversano, Ernesto Fattorusso, Silvana Magno, Lucia Carrano, Maurizio PansiniAbstract:Abstract A detailed analysis of the secondary liposoluble metabolites of the Verongida sponge Aplysina archery has been performed. Ten bromotyrosine derivatives have been identified of which one, 1, is a novel compound. Structure of 1 has been assigned on the basis of spectroscopic evidence including 2D-NMR experiments. Absolute configuration has been suggested by helicity rule.
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chemistry of Verongida sponges vi comparison of the secondary metabolic composition of aplysina insularis and aplysina fulva
Biochemical Systematics and Ecology, 1996Co-Authors: Patrizia Ciminiello, Ernesto Fattorusso, Silvana Magno, Maurizio PansiniAbstract:Abstract Caribbean specimens of the sponge Aplysina insularis have been shown to contain a number of known brominated metabolites and two novel compounds ( 14 and 15 ), whose structures have been determined on the basis of spectral data. The results obtained indicate that the metabolism of A. insularis is rather different from that of A. fulva . This diversity indicates that the two sponges cannot be considered as two formae of the same species ( A. fistularis ), but, in accord with a recent suggestion by Pulitzer-Finali, should be classified as two distinct species.
Patrizia Ciminiello - One of the best experts on this subject based on the ideXlab platform.
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chemistry of Verongida sponges 10 secondary metabolite composition of the caribbean sponge verongula gigantea
Journal of Natural Products, 2000Co-Authors: Patrizia Ciminiello, Carmela Dellaversano, Ernesto Fattorusso, Silvana Magno, Maurizio PansiniAbstract:A detailed analysis of the secondary metabolites of the Caribbean sponge Verongula gigantea has been performed. A number of bromotyrosine derivatives, 1, 2, and 6−17, were identified, one of which (17) is a novel compound. Its structure was determined on the basis of spectroscopic evidence. Additionally, aureol (18) and 5,6-dibromo-N,N-dimethyltryptamine (19) were isolated from one of the five analyzed specimens.
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chemistry of Verongida sponges 9 1 secondary metabolite composition of the caribbean sponge aplysina cauliformis
Journal of Natural Products, 1999Co-Authors: Patrizia Ciminiello, Carmela Dellaversano, Ernesto Fattorusso, Silvana Magno, Maurizio PansiniAbstract:A detailed analysis of the secondary metabolites of the sponge Aplysina cauliformis has been performed. Eight compounds were identified, two of which (13 and 14) are new bromotyrosine derivatives whose structures were determinated from spectroscopic evidence, including 2D NMR. The new compounds were analyzed for cytotoxic activity, and compound 14 was shown to inhibit mammalian protein synthesis and cell proliferation.
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chemistry of Verongida sponges viii1 bromocompounds from the mediterranean sponges aplysina aerophoba and aplysina cavernicola
Tetrahedron, 1997Co-Authors: Patrizia Ciminiello, Ernesto Fattorusso, Silvana Magno, Martino Forino, Maurizio PansiniAbstract:Abstract A detailed analysis of the secondary metabolites of Aplysina aerophoba and Aplysina cavernicola has been performed. A number of bromotyrosine derivatives have been identified of which two, 14 and 15, both isolated from A. cavernicola, are novel compounds. Their structures have been assigned on the basis of spectroscopic and chemical data. The obtained results indicate a remarkable difference in the secondary metabolism of the two sponges, which eventually confirms the separation of the two Mediterranean Aplysina species, in perfect agreement with Vacelet's opinion.
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chemistry of Verongida sponges vii bromocompounds from the caribbean sponge aplysina archeri
Tetrahedron, 1996Co-Authors: Patrizia Ciminiello, Carmela Dellaversano, Ernesto Fattorusso, Silvana Magno, Lucia Carrano, Maurizio PansiniAbstract:Abstract A detailed analysis of the secondary liposoluble metabolites of the Verongida sponge Aplysina archery has been performed. Ten bromotyrosine derivatives have been identified of which one, 1, is a novel compound. Structure of 1 has been assigned on the basis of spectroscopic evidence including 2D-NMR experiments. Absolute configuration has been suggested by helicity rule.
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chemistry of Verongida sponges vi comparison of the secondary metabolic composition of aplysina insularis and aplysina fulva
Biochemical Systematics and Ecology, 1996Co-Authors: Patrizia Ciminiello, Ernesto Fattorusso, Silvana Magno, Maurizio PansiniAbstract:Abstract Caribbean specimens of the sponge Aplysina insularis have been shown to contain a number of known brominated metabolites and two novel compounds ( 14 and 15 ), whose structures have been determined on the basis of spectral data. The results obtained indicate that the metabolism of A. insularis is rather different from that of A. fulva . This diversity indicates that the two sponges cannot be considered as two formae of the same species ( A. fistularis ), but, in accord with a recent suggestion by Pulitzer-Finali, should be classified as two distinct species.
Silvana Magno - One of the best experts on this subject based on the ideXlab platform.
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chemistry of Verongida sponges 10 secondary metabolite composition of the caribbean sponge verongula gigantea
Journal of Natural Products, 2000Co-Authors: Patrizia Ciminiello, Carmela Dellaversano, Ernesto Fattorusso, Silvana Magno, Maurizio PansiniAbstract:A detailed analysis of the secondary metabolites of the Caribbean sponge Verongula gigantea has been performed. A number of bromotyrosine derivatives, 1, 2, and 6−17, were identified, one of which (17) is a novel compound. Its structure was determined on the basis of spectroscopic evidence. Additionally, aureol (18) and 5,6-dibromo-N,N-dimethyltryptamine (19) were isolated from one of the five analyzed specimens.
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chemistry of Verongida sponges 9 1 secondary metabolite composition of the caribbean sponge aplysina cauliformis
Journal of Natural Products, 1999Co-Authors: Patrizia Ciminiello, Carmela Dellaversano, Ernesto Fattorusso, Silvana Magno, Maurizio PansiniAbstract:A detailed analysis of the secondary metabolites of the sponge Aplysina cauliformis has been performed. Eight compounds were identified, two of which (13 and 14) are new bromotyrosine derivatives whose structures were determinated from spectroscopic evidence, including 2D NMR. The new compounds were analyzed for cytotoxic activity, and compound 14 was shown to inhibit mammalian protein synthesis and cell proliferation.
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chemistry of Verongida sponges viii1 bromocompounds from the mediterranean sponges aplysina aerophoba and aplysina cavernicola
Tetrahedron, 1997Co-Authors: Patrizia Ciminiello, Ernesto Fattorusso, Silvana Magno, Martino Forino, Maurizio PansiniAbstract:Abstract A detailed analysis of the secondary metabolites of Aplysina aerophoba and Aplysina cavernicola has been performed. A number of bromotyrosine derivatives have been identified of which two, 14 and 15, both isolated from A. cavernicola, are novel compounds. Their structures have been assigned on the basis of spectroscopic and chemical data. The obtained results indicate a remarkable difference in the secondary metabolism of the two sponges, which eventually confirms the separation of the two Mediterranean Aplysina species, in perfect agreement with Vacelet's opinion.
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chemistry of Verongida sponges vii bromocompounds from the caribbean sponge aplysina archeri
Tetrahedron, 1996Co-Authors: Patrizia Ciminiello, Carmela Dellaversano, Ernesto Fattorusso, Silvana Magno, Lucia Carrano, Maurizio PansiniAbstract:Abstract A detailed analysis of the secondary liposoluble metabolites of the Verongida sponge Aplysina archery has been performed. Ten bromotyrosine derivatives have been identified of which one, 1, is a novel compound. Structure of 1 has been assigned on the basis of spectroscopic evidence including 2D-NMR experiments. Absolute configuration has been suggested by helicity rule.
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chemistry of Verongida sponges vi comparison of the secondary metabolic composition of aplysina insularis and aplysina fulva
Biochemical Systematics and Ecology, 1996Co-Authors: Patrizia Ciminiello, Ernesto Fattorusso, Silvana Magno, Maurizio PansiniAbstract:Abstract Caribbean specimens of the sponge Aplysina insularis have been shown to contain a number of known brominated metabolites and two novel compounds ( 14 and 15 ), whose structures have been determined on the basis of spectral data. The results obtained indicate that the metabolism of A. insularis is rather different from that of A. fulva . This diversity indicates that the two sponges cannot be considered as two formae of the same species ( A. fistularis ), but, in accord with a recent suggestion by Pulitzer-Finali, should be classified as two distinct species.
Ernesto Fattorusso - One of the best experts on this subject based on the ideXlab platform.
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chemistry of Verongida sponges 10 secondary metabolite composition of the caribbean sponge verongula gigantea
Journal of Natural Products, 2000Co-Authors: Patrizia Ciminiello, Carmela Dellaversano, Ernesto Fattorusso, Silvana Magno, Maurizio PansiniAbstract:A detailed analysis of the secondary metabolites of the Caribbean sponge Verongula gigantea has been performed. A number of bromotyrosine derivatives, 1, 2, and 6−17, were identified, one of which (17) is a novel compound. Its structure was determined on the basis of spectroscopic evidence. Additionally, aureol (18) and 5,6-dibromo-N,N-dimethyltryptamine (19) were isolated from one of the five analyzed specimens.
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chemistry of Verongida sponges 9 1 secondary metabolite composition of the caribbean sponge aplysina cauliformis
Journal of Natural Products, 1999Co-Authors: Patrizia Ciminiello, Carmela Dellaversano, Ernesto Fattorusso, Silvana Magno, Maurizio PansiniAbstract:A detailed analysis of the secondary metabolites of the sponge Aplysina cauliformis has been performed. Eight compounds were identified, two of which (13 and 14) are new bromotyrosine derivatives whose structures were determinated from spectroscopic evidence, including 2D NMR. The new compounds were analyzed for cytotoxic activity, and compound 14 was shown to inhibit mammalian protein synthesis and cell proliferation.
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chemistry of Verongida sponges viii1 bromocompounds from the mediterranean sponges aplysina aerophoba and aplysina cavernicola
Tetrahedron, 1997Co-Authors: Patrizia Ciminiello, Ernesto Fattorusso, Silvana Magno, Martino Forino, Maurizio PansiniAbstract:Abstract A detailed analysis of the secondary metabolites of Aplysina aerophoba and Aplysina cavernicola has been performed. A number of bromotyrosine derivatives have been identified of which two, 14 and 15, both isolated from A. cavernicola, are novel compounds. Their structures have been assigned on the basis of spectroscopic and chemical data. The obtained results indicate a remarkable difference in the secondary metabolism of the two sponges, which eventually confirms the separation of the two Mediterranean Aplysina species, in perfect agreement with Vacelet's opinion.
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chemistry of Verongida sponges vii bromocompounds from the caribbean sponge aplysina archeri
Tetrahedron, 1996Co-Authors: Patrizia Ciminiello, Carmela Dellaversano, Ernesto Fattorusso, Silvana Magno, Lucia Carrano, Maurizio PansiniAbstract:Abstract A detailed analysis of the secondary liposoluble metabolites of the Verongida sponge Aplysina archery has been performed. Ten bromotyrosine derivatives have been identified of which one, 1, is a novel compound. Structure of 1 has been assigned on the basis of spectroscopic evidence including 2D-NMR experiments. Absolute configuration has been suggested by helicity rule.
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chemistry of Verongida sponges vi comparison of the secondary metabolic composition of aplysina insularis and aplysina fulva
Biochemical Systematics and Ecology, 1996Co-Authors: Patrizia Ciminiello, Ernesto Fattorusso, Silvana Magno, Maurizio PansiniAbstract:Abstract Caribbean specimens of the sponge Aplysina insularis have been shown to contain a number of known brominated metabolites and two novel compounds ( 14 and 15 ), whose structures have been determined on the basis of spectral data. The results obtained indicate that the metabolism of A. insularis is rather different from that of A. fulva . This diversity indicates that the two sponges cannot be considered as two formae of the same species ( A. fistularis ), but, in accord with a recent suggestion by Pulitzer-Finali, should be classified as two distinct species.
