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Catherine A. Thornton - One of the best experts on this subject based on the ideXlab platform.

  • Evaluation of four Veterinary Hematology analyzers for bovine and ovine blood counts for in vitro testing of medical devices.
    Artificial organs, 2016
    Co-Authors: Ina Laura Pieper, Yasmin Friedmann, Alyssa Jones, Catherine A. Thornton
    Abstract:

    Small affordable automated Hematology analyzers that produce rapid and accurate complete blood cell counts are a valuable tool to researchers developing blood-handling medical devices, such as ventricular assist devices, for in vitro safety assessments. In such studies, it is common to use the blood of large animals such as cattle and sheep. However, the commercially available instruments have not been evaluated for their ability to measure the blood counts of these animals. In this study, we compare, for the first time, four Veterinary analyzers for blood counts on bovine and ovine blood samples. We look at ease of use, repeatability and agreement with a view to inform researchers of the benefits of these instruments in routine measurement of ovine and bovine bloods during in vitro testing. Complete blood cell counts and a three-part differential (granulocytes, monocytes, and lymphocytes) were measured by each of the instruments, and the results compared to those obtained from two additional analyzers used in a reference laboratory. Repeatability and agreement were evaluated using the Bland-Altman method; bias and 95% limits of agreement between the instruments, and between the instruments and two reference instruments, were used to evaluate instrument performance. In summary, there are advantages and disadvantages with all instruments. Of the four instruments tested, the repeatability and agreement was fairly similar for all instruments except one instrument which cannot be recommended for bovine or ovine blood counts.

Ueta M.t. - One of the best experts on this subject based on the ideXlab platform.

  • Hematological Parameters Of Hoplias Malabaricus (characiformes: Erythrinidae) Parasitized By Monogenea In Lagoons In Pirassununga, Brazil [parâmetros Hematológicos De Hoplias Malabaricus (characiformes: Erythrinidae) Parasitados Por Monogenea Em Lago
    2015
    Co-Authors: Correa L.l., Karling L.c., Takemoto R.m., Ceccarelli P.s., Ueta M.t.
    Abstract:

    Hematology is an important pathological and diagnostic tool. This paper describes the hematological parameters of 76 specimens of Hoplias malabaricus infested with monogenean parasites, which were collected from two lagoons. The hematological parameters included: erythrocyte count (Er), microhematocrit (Hct), hemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC). The blood parameters showed no significant changes associated with the infestation. Water temperature was found to be negatively correlated with MCV and Hct, with values of rs= -0.52, p<0.0001 and rs= -0.48, p<0.0001, respectively. The mean Relative Condition Factor was Kn=1.01, indicating good health conditions of the fish in these lagoons. No correlation was found between the monogenean infestation and the blood parameters, or between the environmental factors and the monogeneans.224457462Ballarin, L., Dall'Oro, M., Bertotto, D., Libertini, A., Francescon, A., Barbaro, A., Haematological parameters in Umbrina cirrosa (Teleostei, Sciaenidae): A comparison between diploid and triploid specimens (2004) Comp Biochem Physiol a Mol Integr Physiol, 138 (1), pp. 45-51. , http://dx.doi.org/10.1016/j.cbpb.2004.02.019, PMid:15165570Bolger, T., Connolly, P.L., The selection of suitable indices for the measurement and analysis of fish condition (1989) J Fish Biol, 34 (2), pp. 171-182. , http://dx.doi.org/10.1111/j.1095-8649.1989.tb03300.xBush, A.O., Lafferty, K.D., Lotz, J.M., Shostak, A.W., Parasitology meets ecology on its own terms (1997) J Parasitol, 83 (4), pp. 575-583. , http://dx.doi.org/10.2307/3284227, PMid:9267395: Margolis et al. revisitedCorrêa, L.L., Karling, L.C., Takemoto, R.M., Ceccarelli, P.S., Ueta, M.T., Hematological alterations caused by high intensity of L3 larvae of Contracaecum sp Railliet &Henry, 1912 (Nematoda, Anisakidae) in the stomach of Hoplias malabaricus in lakes in Pirassununga, São Paulo (2013) Parasitol Res, 112 (8), pp. 2783-2789. , http://dx.doi.org/10.1007/s00436-013-3446-8, PMid:23666228Collier, H.B., The standardization of blood haemoglobin determinations (1944) Can Med Assoc J, 50 (6), pp. 550-552. , PMid:20323122 PMCid:PMC1581573Dacie, J.V., Lewis, S.M., (2007) Practical Haematology, , 7th ed. London: Churchill LivingstoneDias, J.L.C., Sinhorini, I.L., Qualitative evaluation of the inflammatory response modulated by temperature in tadpoles of Rana catesbeiana (1991) Cienc Cult, 43 (4), pp. 304-306Goldenfarb, P.B., Bowyer, F.P., Hall, E., Brosious, E., Reproducibility in the Hematology laboratory: The microhematocrit determination (1971) Am J Clin Pathol, 56 (1), pp. 35-39. , PMid:5556212Gomiero, L.M., Braga, F.M.S., Relação peso-comprimento e fator de condição para Cichla cf. ocellaris e Cichla monoculus (Perciformes, Cichlidae) no reservatório de Volta Grande, rio Grande - MG/SP (2003) Acta Sci Biol Sci, 25 (1), pp. 79-86. , http://dx.doi.org/10.4025/actascibiolsci.v25i1.2119Graham, M.S., Haedrich, R.L., Fletcher, G.L., Hematology of three deep-sea fishes: A reflection of low metabolic rates (1985) Comp Biochem Physiol a Comp Physiol, 80 (1), pp. 79-84. , http://dx.doi.org/10.1016/0300-9629(85)90682-6Hrubec, T.C., Smith, S.A., Hematology of fish (1998) Schalm's Veterinary Hematology, pp. 1120-1125. , Feldman BF, Zinkl JG, Jain NC, 5th ed. Sydney: W.W. LippincottHumason, G.L., (1979) Animal Tissue Techniques, , 4th ed. San Francisco: W.H. Freeman and CompanyKhan, R.A., Thulin, J., Influence of pollution on parasites of aquatic animals (1991) Adv Parasitol, 30, pp. 201-238. , http://dx.doi.org/10.1016/S0065-308X(08)60309-7Khan, R.A., Health of flatfish from localities in Placentia Bay, Newfoundland, contaminated with petroleum and PCBs (2003) Arch Environ Contam Toxicol, 44 (4), pp. 485-492. , http://dx.doi.org/10.1007/s00244-002-2063-9, PMid:12712279Kohn, A., Fernandes, B.M.M., Cohen, S.C., (2007) South American Trematodes Parasites of Fishes, , Rio de Janeiro: FiocruzLizama, M.A.P., Takemoto, R.M., Pavanelli, G.C., New species of Tereancistrum Kritsky, Thatcher & Kayton, 1980 (Monogenea: Dactylogyridae: Ancyrocephalinae) from the gills of Prochilodus lineatus (Osteichthyes: Prochilodontidae) from the upper Paraná River floodplain, Brazil (2004) Syst Parasitol, 57 (1), pp. 45-49. , http://dx.doi.org/10.1023/B:SYPA.0000010684.67784.6e, PMid:14739674Mackenzie, K., Williams, H.H., Williams, B., McVicar, A.H., Siddall, R., Parasites as indicators of water quality and the potential use of helminth transmission in marine pollution studies (1995) Adv Parasitol, 35, pp. 85-144. , http://dx.doi.org/10.1016/S0065-308X(08)60070-6Mackenzie, K., Parasites as pollution indicators in marine ecosystems: A proposed early warning system (1999) Mar Pollut Bull, 38 (11), pp. 955-959. , http://dx.doi.org/10.1016/S0025-326X(99)00100-9Madi, R.R., Ueta, M.T., O papel de Ancyrocephalinae (Monogenea: Dactylogyridae), parasito de Geophagus brasiliensis (Pisces: Cichlidae) (2009) Rev Bras Parasitol Vet, 18 (2), pp. 38-41. , http://dx.doi.org/10.4322/rbpv.01802008, 462 Corrêa, L.L. et al. Rev. Bras. Parasitol. Vet. como indicador ambientalMatushima, E.R., Mariano, M., Kinetics of the inflammatory reaction induced by carrageenin in the swinbladder of Oreochromis niloticus (Nile tilapia) (1996) Braz J Vet Res Anim Sci, 33 (1), pp. 5-10Montero, F.E., Crespo, S., Padrós, F., Gándara, F., García, A., Raga, J.A., Effects of the gill parasite Zeuxapta seriolae (Monogenea: Heteraxinidae) on the amberjack Seriola dumerili Risso (Teleostei: Carangidae) (2004) Aquaculture, 232 (1-4), pp. 153-163. , http://dx.doi.org/10.1016/S0044-8486(03)00536-2Moles, A., Wade, T.L., Parasitism and phagocytic function among sand lance Ammodytes hexapterus Pallas exposed to crude oil-laden sediments (2001) Bull Environ Contam Toxicol, 66 (4), pp. 528-535. , http://dx.doi.org/10.1007/s00128-001-0039-5Nakatani, K., Agostinho, A.A., Baumgartner, G., Bialetzki, A., Sanches, P.V., Makrakis, M.C., (2001) Ordem Characiformes. In: Ovos E Larvas De Peixes De Água Doce: Desenvolvimento E Manual De Identificação, pp. 73-220. , Maringá: UEMPaiva, M.J.T.R., Ishikawa, C.M., Campos, B.E.S., Eiras, A.C., Haematological characteristics associated with parasitism in mullets, Mugil platanus Günther, from the estuarine region of Cananéia (1997) São Paulo, Brazil. Rev Bras Zool, 14 (2), pp. 329-339. , http://dx.doi.org/10.1590/S0101-81751997000200007Paiva, M.J.T.R., Ishikawa, C.M., Eiras, A.A., Felizardo, N.N., Haemotological analysis of Chara, Pseudoplatystoma fasciatum in captivity (2000) Eur Aquacult Soc, 28, p. 590. , Aqua 2000. Responsible aquaculture in the new millennium. Nice, France. May 2-6Paiva, M.J.T.R., Souza, A.T.S., Pavanelli, G.C., Takemoto, R.M., Eiras, A.C., Hematological evaluation in commercial fish species from the floodplin of the upper Paraná River, Brazil (2000) Acta Sci Biol Sci, 22 (2), pp. 507-513Paiva, M.J.T.R., Tavares-Dias, M., Eritrograma, relação viscerosomática, hepatosomática e esplenosomática em tainhas Mugil platanus Günther (Osteichthyes, Mugilidae) parasitadas (2002) Rev Bras Zool, 19 (3), pp. 807-818. , http://dx.doi.org/10.1590/S0101-81752002000300019Rio-Zaragoza, O.B., Avila, E.J.F., Rueda, P.A., Haematological and gill responses to an experimental infection of Dactylogyrid monogeneans on the spotted rose snapper Lutjanus guttatus (Steidachner, 1869) (2010) Aquacult Res, 41 (11), pp. 1592-1601. , http://dx.doi.org/10.1111/j.1365-2109.2009.02471.xRosim, D.F., Mendoza-Franco, E.F., Luque, J.L., New and previously described species of Urocleidoides (Monogenoidea: Dactylogyridae) infecting the gills and nasal cavities of Hoplias malabaricus (Characiformes: Erythrinidae) from Brazil (2011) J Parasitol, 97 (3), pp. 406-417. , http://dx.doi.org/10.1645/GE-2593.1, PMid:21506767Shibatta, O.A., Orsi, M.L., Bennemann, S.T., Silva-Souza, A.T., (2002) Diversidade E Distribuição De Peixes Na Bacia Do Rio Tibagi, pp. 403-423. , In: Medri ME, Bianchini E, Shibatta OA, Pimenta JA, editores. A bacia do rio Tibagi. Londrina: EDUELSilvano, R.A.M., Oyakawa, O.T., Amaral, B.D., Begossi, A., (2001) Peixes Do Alto Rio Juruá (Amazônia, Brasil). São Paulo, , Universidade de São Paulo, Imprensa Oficial do Estado de Paulo, PMid:11677442(1996) SAS User's Guide: Statistcs, , Statistical Analisys System Institute - SAS, Release 6.12. North Caroline: CoryTaphorn, D.C., (1992) The Characiform Fishes of the Apure River Drainage, , Venezuela. Guanare: BioLlania-Edición Especial, Monografias Cientificas del Museo de Ciencias NaturalesTavares-Dias, M., Sandrim, E.F.S., Sandrim, A., Características hematológicas do tambaqui (Colossoma macropomum) (1998) Rev Bras Biol, 58 (2), pp. 197-202. , Cuvier, 1818 (Osteichthyes: Characidae) em sistema de monocultivo intensivo. I. Série EritrocitáriaTavares-Dias, M., Sandrim, E.F.S., Sandrim, A., Características hematológicas do tambaqui (Colossoma macropomum) Cuvier (1998) Rev Bras Biol, 58 (2), pp. 197-202. , 1818 (Osteichthyes: Characidae) em sistema de monocultivo intensivo. I. Série EritrocitáriaTavares-Dias, M., Martins, M.L., Kronka, S.N., Evaluation of the haematological parameters in Piaractus mesopotamicus Holmberg (Osteichthyes: Characidae) with Argulus sp. (Crustacea, Branchiura) infestation and treatment with organophosphate (1999) Rev Bras Zool, 16 (2), pp. 553-555. , http://dx.doi.org/10.1590/S0101-81751999000200019Tavares-Dias, M., Schalch, S.H.C., Martins, M.L., Hematologia de teleósteos brasileiros com infecção parasitária (1999) Acta Sci Biol Sci, 21 (2), pp. 337-342. , I. Variáveis do Leporinus macrocephalus Garavello & Britski, 1988 (Anostomidae) e Piaractus mesopotamicus Holmberg, 1887 (Characidae)Tavares-Dias, M., Moraes, F.R., Martins, M.L., Santana, A.E., Haematological changes in Oreochromis niloticus (Osteichthyes: Cichlidae) with gill ichthyophthiriasis and saprolegniosis (2002) Bol Inst Pesca, 28 (1), pp. 1-9Tavares-Dias, M., Moraes, F.R., (2004) Hematologia De Peixes Teleósteos, , Ribeirão Preto: VillimpressTavares-Dias, M., Moraes, F.R., Leukocyte and thrombocyte reference values for channel catfish (Ictalurus punctatus Raf.), with an assessment of morphologic, cytochemical, and ultrastructural features (2007) Vet Clin Pathol, 36 (1), pp. 49-54. , http://dx.doi.org/10.1111/j.1939-165X.2007.tb00181.x, PMid:17311194Thatcher, V.E., Patologia de peixes da Amazônia brasileira (1981) Aspectos gerais Acta Amaz, 11 (1), pp. 125-140Ueda, I.K., Egami, M.I., Sasso, W.S., Matushima, E.R., Estudos hematológicos em Oreochromis niloticus (Linnaeus,1758) (Cichilidae, Teleostei)-Parte I (1997) Braz J Vet Res Anim Sci, 34 (5), pp. 270-275van Vuren, J.H.J., The effects of toxicants on the haematology of Labeo umbratus (Teleostei: Cyprinidae) (1986) Comp Biochem Physiol C, 83 (1), pp. 155-159. , http://dx.doi.org/10.1016/0742-8413(86)90029-0Viljoen, B.C.S., Van Vuren, J.H.J., Physical characteristics of the blood plasma of Labeo ruddi and Labeo rosae (Pisces: Cyprinidae) (1991) Comp Biochem Physiol a Comp Physiol, 100 (4), pp. 873-875Wells, R.M.G., Baldwin, J., Seymour, R.S., Christian, K., Brittain, T., Red blood cell function and haematology in two tropical freshwater fishes from Australia (2005) Comp Biochem Physiol a Mol Integr Physiol, 141 (1), pp. 87-93. , http://dx.doi.org/10.1016/j.cbpb.2005.04.005, PMid:1589394

John W. Harvey - One of the best experts on this subject based on the ideXlab platform.

Ina Laura Pieper - One of the best experts on this subject based on the ideXlab platform.

  • Evaluation of four Veterinary Hematology analyzers for bovine and ovine blood counts for in vitro testing of medical devices.
    Artificial organs, 2016
    Co-Authors: Ina Laura Pieper, Yasmin Friedmann, Alyssa Jones, Catherine A. Thornton
    Abstract:

    Small affordable automated Hematology analyzers that produce rapid and accurate complete blood cell counts are a valuable tool to researchers developing blood-handling medical devices, such as ventricular assist devices, for in vitro safety assessments. In such studies, it is common to use the blood of large animals such as cattle and sheep. However, the commercially available instruments have not been evaluated for their ability to measure the blood counts of these animals. In this study, we compare, for the first time, four Veterinary analyzers for blood counts on bovine and ovine blood samples. We look at ease of use, repeatability and agreement with a view to inform researchers of the benefits of these instruments in routine measurement of ovine and bovine bloods during in vitro testing. Complete blood cell counts and a three-part differential (granulocytes, monocytes, and lymphocytes) were measured by each of the instruments, and the results compared to those obtained from two additional analyzers used in a reference laboratory. Repeatability and agreement were evaluated using the Bland-Altman method; bias and 95% limits of agreement between the instruments, and between the instruments and two reference instruments, were used to evaluate instrument performance. In summary, there are advantages and disadvantages with all instruments. Of the four instruments tested, the repeatability and agreement was fairly similar for all instruments except one instrument which cannot be recommended for bovine or ovine blood counts.

Timothy Sheahan - One of the best experts on this subject based on the ideXlab platform.

  • increasing the translation of mouse models of mers coronavirus pathogenesis through kinetic hematological analysis
    PLOS ONE, 2019
    Co-Authors: Sarah R Leist, Kara Jensen, Ralph S Baric, Timothy Sheahan
    Abstract:

    Newly emerging viral pathogens pose a constant and unpredictable threat to human and animal health. Coronaviruses (CoVs) have a penchant for sudden emergence, as evidenced by severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome CoV (MERS-CoV) and most recently, swine acute diarrhea syndrome coronavirus (SADS-CoV). Small animal models of emerging viral pathogenesis are crucial to better understand the virus and host factors driving disease progression. However, rodent models are often criticized for their limited translatability to humans. The complete blood count is the most ordered clinical test in the United States serving as the cornerstone of clinical medicine and differential diagnosis. We recently generated a mouse model for MERS-CoV pathogenesis through the humanization of the orthologous entry receptor dipeptidyl peptidase 4 (DPP4). To increase the translatability of this model, we validated and established the use of an automated Veterinary Hematology analyzer (VetScan HM5) at biosafety level 3 for analysis of peripheral blood. MERS-CoV lung titer peaked 2 days post infection concurrent with lymphopenia and neutrophilia in peripheral blood, two phenomena also observed in MERS-CoV infection of humans. The fluctuations in leukocyte populations measured by Vetscan HM5 were corroborated by standard flow cytometry, thus confirming the utility of this approach. Comparing a sublethal and lethal dose of MERS-CoV in mice, analysis of daily blood draws demonstrates a dose dependent modulation of leukocytes. Major leukocyte populations were modulated before weight loss was observed. Importantly, neutrophil counts on 1dpi were predictive of disease severity with a lethal dose of MERS-CoV highlighting the predictive value of Hematology in this model. Taken together, the inclusion of hematological measures in mouse models of emerging viral pathogenesis increases their translatability and should elevate the preclinical evaluation of MERS-CoV therapeutics and vaccines to better mirror the complexity of the human condition.