The Experts below are selected from a list of 312 Experts worldwide ranked by ideXlab platform
Vengadesh Letchumanan - One of the best experts on this subject based on the ideXlab platform.
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prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from retail shrimps in malaysia
Frontiers in Microbiology, 2015Co-Authors: Vengadesh Letchumanan, Kokgan ChanAbstract:Vibrio parahaemolyticus is a marine and estuarine bacterium that has been the leading cause of foodborne outbreaks which leads to a significant threat to human health worldwide. Consumption of seafood contaminated with Vibrio parahaemolyticus causes acute gastroenteritis in individuals. The bacterium poses two main virulence factor including the thermostable direct hemolysin (tdh) which is a pore-forming protein that contributes to the invasiveness of the bacterium in humans and TDH-related hemolysin (trh), which plays a similar role as thermostable direct hemolysin (tdh) in the disease pathogenesis. This study aimed to investigate the antimicrobial resistance Vibrio parahaemolyticus strains in shrimps purchased from wetmarkets and supermarkets. The toxR-based PCR assay indicated that a total of 57.8% (185/320) isolates were positive for V. parahaemolyticus. Only 10% (19/185) toxR-positive isolate exhibit the TDH-related hemolysin (trh) gene and none of the isolates were tested positive for thermostable direct hemolysin (tdh). The MAR index was measured for 14 common antimicrobial agents. The results indicated 98% of the isolates were highly susceptible to imipenem, ampicillin sulbactam (96%), chloramphenicol (95%), trimethoprim-sulfamet (93%), gentamicin (85%), levofloxacin (83%) and tetracycline (82%). The chloramphenicol (catA2) and kanamycin (aphA-3) resistance genes were detected in the resistant V. parahaemolyticus isolates. Our results demonstrate that shrimps are contaminated with V. parahaemolyticus, some of which carry the trh-gene thus being potential to cause food borne illness. The occurrence of multidrug resistance strains in the environment could be an indication of excessive usage of antibiotics in agriculture and aquaculture fields.
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Vibrio parahaemolyticus a review on the pathogenesis prevalence and advance molecular identification techniques
Frontiers in Microbiology, 2014Co-Authors: Vengadesh Letchumanan, Kokgan ChanAbstract:Vibrio parahaemolyticus is a Gram-negative halophilic bacterium that is found in estuarine, marine and coastal environments. Vibrio parahaemolyticus is the leading causal agent of human acute gastroenteritis following the consumption of raw, undercooked or mishandled marine products. In rare cases, Vibrio parahaemolyticus causes wound infection, ear infection or septicaemia in individuals with pre-existing medical conditions. Vibrio parahaemolyticus has two hemolysins virulence factors that are thermostable direct hemolysin (tdh)-a pore-forming protein that contributes to the invasiveness of the bacterium in humans, and TDH-related hemolysin (trh), which plays a similar role as thermostable direct hemolysin (tdh) in the disease pathogenesis. In addition, the bacterium is also encodes for adhesions and type III secretion systems (T3SS1 and T3SS2) to ensure its survival in the environment. This review aims at discussing the Vibrio parahemolyticus growth and characteristics, pathogenesis, prevalence and advances in molecular identification techniques.
Kim Orth - One of the best experts on this subject based on the ideXlab platform.
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virulence determinants for Vibrio parahaemolyticus infection
Current Opinion in Microbiology, 2013Co-Authors: Lingling Zhang, Kim OrthAbstract:Vibrio parahaemolyticus is a marine microorganism that causes acute gastroenteritis associated with the consumption of contaminated raw or under cooked seafood. During infection, the bacterium utilizes a wide variety of virulence factors, including adhesins, toxins and type III secretion systems, to cause both cytotoxicity in cultured cells and enterotoxicity in animal models. Herein, we describe recent discoveries on the regulation and characterization of the virulence factors from V. para. Determining how this bacterial pathogen uses virulence factors to mediate pathogenicity improves our understanding of V. para. infections and more generally, host–pathogen interactions.
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Vibrio parahaemolyticus cell biology and pathogenicity determinants
Microbes and Infection, 2011Co-Authors: Christopher A Broberg, Thomas Calder, Kim OrthAbstract:Vibrio parahaemolyticus is a significant cause of gastroenteritis worldwide. Characterization of this pathogen has revealed a unique repertoire of virulence factors that allow for colonization of the human host and disease. The following describes the known pathogenicity determinants while establishing the need for continued research.
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Acanthamoeba castellanii promotes the survival of Vibrio parahaemolyticus.
Applied and Environmental Microbiology, 2008Co-Authors: Michelle A. Laskowski-arce, Kim OrthAbstract:Vibrio parahaemolyticus is a food-borne pathogen that naturally inhabits both marine and estuarine environments. Free-living protozoa exist in similar aquatic environments and function to control bacterial numbers by grazing on free-living bacteria. Protozoa also play an important role in the survival and spread of some pathogenic species of bacteria. We investigated the interaction between the protozoan Acanthamoeba castellanii and the bacterium Vibrio parahaemolyticus. We found that Acanthamoeba castellanii does not prey on Vibrio parahaemolyticus but instead secretes a factor that promotes the survival of Vibrio parahaemolyticus in coculture. These studies suggest that protozoa may provide a survival advantage to an extracellular pathogen in the environment.
Kokgan Chan - One of the best experts on this subject based on the ideXlab platform.
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prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from retail shrimps in malaysia
Frontiers in Microbiology, 2015Co-Authors: Vengadesh Letchumanan, Kokgan ChanAbstract:Vibrio parahaemolyticus is a marine and estuarine bacterium that has been the leading cause of foodborne outbreaks which leads to a significant threat to human health worldwide. Consumption of seafood contaminated with Vibrio parahaemolyticus causes acute gastroenteritis in individuals. The bacterium poses two main virulence factor including the thermostable direct hemolysin (tdh) which is a pore-forming protein that contributes to the invasiveness of the bacterium in humans and TDH-related hemolysin (trh), which plays a similar role as thermostable direct hemolysin (tdh) in the disease pathogenesis. This study aimed to investigate the antimicrobial resistance Vibrio parahaemolyticus strains in shrimps purchased from wetmarkets and supermarkets. The toxR-based PCR assay indicated that a total of 57.8% (185/320) isolates were positive for V. parahaemolyticus. Only 10% (19/185) toxR-positive isolate exhibit the TDH-related hemolysin (trh) gene and none of the isolates were tested positive for thermostable direct hemolysin (tdh). The MAR index was measured for 14 common antimicrobial agents. The results indicated 98% of the isolates were highly susceptible to imipenem, ampicillin sulbactam (96%), chloramphenicol (95%), trimethoprim-sulfamet (93%), gentamicin (85%), levofloxacin (83%) and tetracycline (82%). The chloramphenicol (catA2) and kanamycin (aphA-3) resistance genes were detected in the resistant V. parahaemolyticus isolates. Our results demonstrate that shrimps are contaminated with V. parahaemolyticus, some of which carry the trh-gene thus being potential to cause food borne illness. The occurrence of multidrug resistance strains in the environment could be an indication of excessive usage of antibiotics in agriculture and aquaculture fields.
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Vibrio parahaemolyticus a review on the pathogenesis prevalence and advance molecular identification techniques
Frontiers in Microbiology, 2014Co-Authors: Vengadesh Letchumanan, Kokgan ChanAbstract:Vibrio parahaemolyticus is a Gram-negative halophilic bacterium that is found in estuarine, marine and coastal environments. Vibrio parahaemolyticus is the leading causal agent of human acute gastroenteritis following the consumption of raw, undercooked or mishandled marine products. In rare cases, Vibrio parahaemolyticus causes wound infection, ear infection or septicaemia in individuals with pre-existing medical conditions. Vibrio parahaemolyticus has two hemolysins virulence factors that are thermostable direct hemolysin (tdh)-a pore-forming protein that contributes to the invasiveness of the bacterium in humans, and TDH-related hemolysin (trh), which plays a similar role as thermostable direct hemolysin (tdh) in the disease pathogenesis. In addition, the bacterium is also encodes for adhesions and type III secretion systems (T3SS1 and T3SS2) to ensure its survival in the environment. This review aims at discussing the Vibrio parahemolyticus growth and characteristics, pathogenesis, prevalence and advances in molecular identification techniques.
Liv Marit Rorvik - One of the best experts on this subject based on the ideXlab platform.
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occurrence of Vibrio parahaemolyticus v cholerae and v vulnificus in norwegian blue mussels mytilus edulis
Applied and Environmental Microbiology, 2006Co-Authors: Anette Bauer, Oyvin Ostensvik, Malin Florvag, Oyvind Ormen, Liv Marit RorvikAbstract:Vibrio parahaemolyticus, V. cholerae, and V. vulnificus were isolated from 10.3%, 1.0%, and 0.1% of 885 blue mussel samples, respectively. Four of the samples contained trh+ V. parahaemolyticus, while no tdh-positive isolates were detected. The V. cholerae isolates were non-O:1/non-O:139 serotypes and were ctxA negative.
Anette Bauer - One of the best experts on this subject based on the ideXlab platform.
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occurrence of Vibrio parahaemolyticus v cholerae and v vulnificus in norwegian blue mussels mytilus edulis
Applied and Environmental Microbiology, 2006Co-Authors: Anette Bauer, Oyvin Ostensvik, Malin Florvag, Oyvind Ormen, Liv Marit RorvikAbstract:Vibrio parahaemolyticus, V. cholerae, and V. vulnificus were isolated from 10.3%, 1.0%, and 0.1% of 885 blue mussel samples, respectively. Four of the samples contained trh+ V. parahaemolyticus, while no tdh-positive isolates were detected. The V. cholerae isolates were non-O:1/non-O:139 serotypes and were ctxA negative.
