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Alfred Puhler - One of the best experts on this subject based on the ideXlab platform.
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the broad bean gene vfnod32 encodes a nodulin with sequence similarities to chitinases that is homologous to alpha beta 8 barrel type seed proteins
Plant Physiology, 1996Co-Authors: Andreas M Perlick, Martin Fruhling, Gerald Schroder, S C Frosch, Alfred PuhlerAbstract:Nodulin gene transcripts isolated from a broad bean (Vicia faba L.) root nodule cDNA library and designated VfNOD32 are detectable in the nitrogen-fixing zone III of nodules and in much smaller amounts in flowers. In nodules, these transcripts are detectable for the first time 7 d after inoculation, at least 1 d before leghemoglobin gene transcription starts. Two putative full-length cDNAs representing different transcript sequences of 92.5% identity were sequenced. The corresponding broad bean genes were termed VfNOD32-A1 and VfNOD32-A2, and the encoded proteins were termed Nvf32-A1 and Nvf32-A2. The derived amino acid sequences of the Nvf32 proteins are highly homologous to the Vicia narbonensis ([alpha]/[beta])8-barrel seed protein narbonin. Considering this homology, Nvf32 is assumed to have a similar structure consisting of [beta]-sheets forming a central barrel surrounded by [alpha]-helices. The two Nvf32 sequences also contain two conserved amino acid motifs that are characteristic of class-III chitinases. Several amino acids demonstrated to be essential for chitinase activity are conserved in both regions, whereas one essential glutamic acid was changed to glycine in the Nvf32-A1 isoform but not in the Nvf32-A2 isoform.
Andreas M Perlick - One of the best experts on this subject based on the ideXlab platform.
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the broad bean gene vfnod32 encodes a nodulin with sequence similarities to chitinases that is homologous to alpha beta 8 barrel type seed proteins
Plant Physiology, 1996Co-Authors: Andreas M Perlick, Martin Fruhling, Gerald Schroder, S C Frosch, Alfred PuhlerAbstract:Nodulin gene transcripts isolated from a broad bean (Vicia faba L.) root nodule cDNA library and designated VfNOD32 are detectable in the nitrogen-fixing zone III of nodules and in much smaller amounts in flowers. In nodules, these transcripts are detectable for the first time 7 d after inoculation, at least 1 d before leghemoglobin gene transcription starts. Two putative full-length cDNAs representing different transcript sequences of 92.5% identity were sequenced. The corresponding broad bean genes were termed VfNOD32-A1 and VfNOD32-A2, and the encoded proteins were termed Nvf32-A1 and Nvf32-A2. The derived amino acid sequences of the Nvf32 proteins are highly homologous to the Vicia narbonensis ([alpha]/[beta])8-barrel seed protein narbonin. Considering this homology, Nvf32 is assumed to have a similar structure consisting of [beta]-sheets forming a central barrel surrounded by [alpha]-helices. The two Nvf32 sequences also contain two conserved amino acid motifs that are characteristic of class-III chitinases. Several amino acids demonstrated to be essential for chitinase activity are conserved in both regions, whereas one essential glutamic acid was changed to glycine in the Nvf32-A1 isoform but not in the Nvf32-A2 isoform.
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The broad bean gene VfNOD32 encodes a nodulin with sequence similarities to chitinases that is homologous to (alpha/beta)(8)-barrel-type seed proteins
'American Society of Plant Biologists (ASPB)', 1996Co-Authors: Andreas M Perlick, S C Frosch, Frühling M, Schröder G, Pühler AlfredAbstract:Perlick AM, Fruhling M, Schroder G, Frosch SC, Pühler A. The broad bean gene VfNOD32 encodes a nodulin with sequence similarities to chitinases that is homologous to (alpha/beta)(8)-barrel-type seed proteins. PLANT PHYSIOLOGY. 1996;110(1):147-154.Nodulin gene transcripts isolated from a broad bean (Vicia faba L.) root nodule cDNA library and designated VfNOD32 are detectable in the nitrogen-fixing zone III of nodules and in much smaller amounts in flowers. In nodules, these transcripts are detectable for the first time 7 d after inoculation, at least 1 d before leghemoglobin gene transcription starts. Two putative full-length cDNAs representing different transcript sequences of 92.5% identity were sequenced. The corresponding broad bean genes were termed VfNOD32-A1 and VfNOD32-A2, and the encoded proteins were termed Nvf32-A1 and Nvf32-A2. The derived amino acid sequences of the Nvf32 proteins are highly homologous to the Vicia narbonensis (alpha/beta)(8)-barrel seed protein narbonin. Considering this homology, Nvf32 is assumed to have a similar structure consisting of beta-sheets forming a central barrel surrounded by alpha-helices. The two Nvf32 sequences also contain two conserved amino acid motifs that are characteristic of class-III chitinases. Several amino acids demonstrated to be essential for chitinase activity are conserved in both regions, whereas one essential glutamic acid was changed to glycine in the Nvf32-A1 isoform but not in the Nvf32-A2 isoform
S C Frosch - One of the best experts on this subject based on the ideXlab platform.
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the broad bean gene vfnod32 encodes a nodulin with sequence similarities to chitinases that is homologous to alpha beta 8 barrel type seed proteins
Plant Physiology, 1996Co-Authors: Andreas M Perlick, Martin Fruhling, Gerald Schroder, S C Frosch, Alfred PuhlerAbstract:Nodulin gene transcripts isolated from a broad bean (Vicia faba L.) root nodule cDNA library and designated VfNOD32 are detectable in the nitrogen-fixing zone III of nodules and in much smaller amounts in flowers. In nodules, these transcripts are detectable for the first time 7 d after inoculation, at least 1 d before leghemoglobin gene transcription starts. Two putative full-length cDNAs representing different transcript sequences of 92.5% identity were sequenced. The corresponding broad bean genes were termed VfNOD32-A1 and VfNOD32-A2, and the encoded proteins were termed Nvf32-A1 and Nvf32-A2. The derived amino acid sequences of the Nvf32 proteins are highly homologous to the Vicia narbonensis ([alpha]/[beta])8-barrel seed protein narbonin. Considering this homology, Nvf32 is assumed to have a similar structure consisting of [beta]-sheets forming a central barrel surrounded by [alpha]-helices. The two Nvf32 sequences also contain two conserved amino acid motifs that are characteristic of class-III chitinases. Several amino acids demonstrated to be essential for chitinase activity are conserved in both regions, whereas one essential glutamic acid was changed to glycine in the Nvf32-A1 isoform but not in the Nvf32-A2 isoform.
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The broad bean gene VfNOD32 encodes a nodulin with sequence similarities to chitinases that is homologous to (alpha/beta)(8)-barrel-type seed proteins
'American Society of Plant Biologists (ASPB)', 1996Co-Authors: Andreas M Perlick, S C Frosch, Frühling M, Schröder G, Pühler AlfredAbstract:Perlick AM, Fruhling M, Schroder G, Frosch SC, Pühler A. The broad bean gene VfNOD32 encodes a nodulin with sequence similarities to chitinases that is homologous to (alpha/beta)(8)-barrel-type seed proteins. PLANT PHYSIOLOGY. 1996;110(1):147-154.Nodulin gene transcripts isolated from a broad bean (Vicia faba L.) root nodule cDNA library and designated VfNOD32 are detectable in the nitrogen-fixing zone III of nodules and in much smaller amounts in flowers. In nodules, these transcripts are detectable for the first time 7 d after inoculation, at least 1 d before leghemoglobin gene transcription starts. Two putative full-length cDNAs representing different transcript sequences of 92.5% identity were sequenced. The corresponding broad bean genes were termed VfNOD32-A1 and VfNOD32-A2, and the encoded proteins were termed Nvf32-A1 and Nvf32-A2. The derived amino acid sequences of the Nvf32 proteins are highly homologous to the Vicia narbonensis (alpha/beta)(8)-barrel seed protein narbonin. Considering this homology, Nvf32 is assumed to have a similar structure consisting of beta-sheets forming a central barrel surrounded by alpha-helices. The two Nvf32 sequences also contain two conserved amino acid motifs that are characteristic of class-III chitinases. Several amino acids demonstrated to be essential for chitinase activity are conserved in both regions, whereas one essential glutamic acid was changed to glycine in the Nvf32-A1 isoform but not in the Nvf32-A2 isoform
Pedraza Verónica - One of the best experts on this subject based on the ideXlab platform.
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Vicia narbonensis-Avena strigosa mixture, a viable alternative in rainfed cropping systems under Mediterranean conditions
'Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA)', 2018Co-Authors: Pedraza Verónica, Perea Francisco, Saavedra Milagros, Fuentes Mariano, Alcántara CristinaAbstract:The demand of vegetable protein for animal feed and the need to diversify the crop rotation in rainfed Mediterranean climates has led to study the viability as new forage crop of the Vicia narbonensis-Avena strigosa mixture. Therefore, a 3-year field trial was conducted at two different and representative locations of the area to evaluate the capacity of both species to form a balanced mixture and to define its potential for high yield and forage quality. Different seeding ratios (65:35, 50:50 and 35:65) were compared with their pure stands and the standard mixture Avena sativa-Vicia sativa (65:35). Forage mixtures establishment and growth varied according to the environmental conditions with a negative influence of a dry year 1 for legumes (<300 mm) and a wet year 2 for oats (>630 mm). However, competition ratio indicated that there were not significant differences between mixed treatments, displaying intermediate ground coverage values, dry matter yield and crude protein regarding pure stands. Higher dry matter yield than control mixture in addition to similar crude protein, acid-detergent fibre, neutral-detergent fibre and digestible dry matter values, were produced at 35:65 in dry years and 65:35 and 50:50 in rainy years with loamy and clay soils, respectively. The appropriate development of both species in the mixture at different soil and rainfall conditions, as well as a good yield often higher than control mixture and a great forage quality, confirm to the narbon bean-black oat mixture as a viable and profitable crop alternative in rainfed cropping systems under Mediterranean conditions
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New strategies for sustainable management of the traditional durum wheat-sunflower rotation: introducing cover crops and alternative crops
Universidad de Córdoba UCOPress, 2018Co-Authors: Pedraza VerónicaAbstract:La rotación trigo-girasol ha constituido la alternativa tradicional prioritaria en las explotaciones agrarias de cultivos herbáceos de secano del sur de España. Los cada vez más exigentes requerimientos medioambientales de la Política Agraria Común y los nuevos retos de la agricultura del siglo XXI, son aspectos prioritarios que marcan la toma de decisiones de los agricultores respecto al manejo de las explotaciones. Actualmente, la adaptación a estos nuevos desafíos implica llevar a cabo prácticas agronómicas sostenibles así como la diversificación de cultivos en las rotaciones, sin embargo hasta el momento, no ha habido alternativas claras a la rotación tradicional trigo-girasol que superen su rentabilidad. El objetivo principal de esta tesis ha sido proponer alternativas sostenibles a la rotación trigo duro-girasol en los secanos andaluces, mediante la introducción de los cultivos intercalares mostaza blanca (Sinapis alba subsp. mairei) y alberjón (Vicia narbonensis L.) y nuevos cultivos como la mezcla forrajera alberjón-avena negra (Vicia narbonensis-Avena strigosa). Para abordar su estudio se han realizado diferentes ensayos de campo y en condiciones controladas entre los años 2011 y 2016 en varias localidades de la provincia de Córdoba (Córdoba y Santa Cruz) y Sevilla (Carmona). Este trabajo ha puesto de manifiesto que los cultivos intercalares introducen mejoras respecto a la conservación de suelo, el control de malas hierbas, la incidencia del jopo de girasol (Orobanche cumana), la calidad del trigo duro y tiene un efecto positivo en el contenido de humedad y fertilidad del suelo tras su incorporación en la capa más superficial en comparación con sistemas de mínimo laboreo y siembra directa. No obstante, estos cultivos también presentan limitaciones, y precisan de un manejo adecuado que hacen necesarios estudios a más largo plazo para maximizar los beneficios de esta práctica agrícola dentro los sistemas de cultivo de secano, especialmente del girasol. Por su parte, el estudio de la nueva mezcla forrajera ha mostrado su viabilidad como cultivo mixto por el adecuado desarrollo de ambas especies en la mezcla y la obtención de rendimientos superiores a la mezcla estándar veza común-avena común, así como por presentar un similar contenido en proteínas y otros parámetros de calidad. Además, la contribución de nitrógeno realizada al sistema permite considerar este cultivo como una futura alternativa en las rotaciones, obteniéndose los mejores resultados a las dosis de siembra de 49 kg/ha de alberjón y 45.5 kg/ha de avena negra en años secos, y a las dosis de 91 kg/ha combinada con 24.5 kg/ha y 70 kg/ha combinada con 35 kg/ha en años con mayor precipitación acumulada en suelos francos y arcillosos, respectivamente. Nuestro estudio abre una nueva línea de investigación prometedora para el futuro de los cultivos herbáceos, y pone de manifiesto que tanto los cultivos intercalares como la mezcla forrajera son alternativas económica y medioambientalmente viables en la agricultura de secano de clima mediterráneo.Durum wheat-sunflower rotation is a common practice in rainfed arable areas in southern Spain. However, the increasingly demanding environmental requirements of the Common Agricultural Policy and the new challenges for food and agriculture in the 21st century are important aspects influencing farmers' decision making each growing season. Nowadays, adapting the traditional rotation and the soil management practices to face these goals involves a sustainable management as well as a crop diversification. However, the lack of profitable alternatives makes future adoption difficult for Spanish arable crop farmers. The present thesis aims to assess the introduction of new crops and cover crops as sustainable alternatives to soil management system in traditional rainfed rotations, by inserting white mustard (Sinapis alba subsp. mairei) and narbon bean (Vicia narbonensis L.) cover crops and the new forage mixture narbon bean-black oat (Vicia narbonensis-Avena strigosa). To that end, a range of experiments has been carried out under field and controlled conditions at different locations of the province of Córdoba (Córdoba y Santa Cruz) and Sevilla (Carmona) between 2011-2016. The short-term results suggested that the tested cover crops may introduce improvements in soil protection, weed and sunflower broomrape control, increased durum wheat quality and higher soil moisture content and fertility after cover crops incorporation into the uppermost layer than other soil management systems such as reduced tillage or no tillage. However, these crops also have limitations and require a proper management that makes necessary further research to analyze the long-term effects and maximize the benefits of this practice in rainfed arable systems, especially in sunflower growth and yields. Results for the forage mixture showed that both species can form a balanced forage mixture for livestock diets, displaying higher dry matter yields and crude protein yield than common vetch-common oat traditional mixture, as well as similar crude protein content and other quality traits. Furthermore, the nitrogen contribution made to the system allows this mixture to be considered as a future alternative in crop rotations, obtaining the best results at a seeding rate of 49 kg/ha for narbon bean and 45.5 for black oat in dry years and at the seeding rates 91 and 24.5 and 70 and 35 kg/ha in rainy years with loamy and clay soils, respectively. Our results open a path of study for the future of arable crops and show that both cover crops and forage mixtures could be economically and environmentally promising crop alternatives in the traditional rotation under the Mediterranean rainfed conditions of southern Spain
Gerald Schroder - One of the best experts on this subject based on the ideXlab platform.
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the broad bean gene vfnod32 encodes a nodulin with sequence similarities to chitinases that is homologous to alpha beta 8 barrel type seed proteins
Plant Physiology, 1996Co-Authors: Andreas M Perlick, Martin Fruhling, Gerald Schroder, S C Frosch, Alfred PuhlerAbstract:Nodulin gene transcripts isolated from a broad bean (Vicia faba L.) root nodule cDNA library and designated VfNOD32 are detectable in the nitrogen-fixing zone III of nodules and in much smaller amounts in flowers. In nodules, these transcripts are detectable for the first time 7 d after inoculation, at least 1 d before leghemoglobin gene transcription starts. Two putative full-length cDNAs representing different transcript sequences of 92.5% identity were sequenced. The corresponding broad bean genes were termed VfNOD32-A1 and VfNOD32-A2, and the encoded proteins were termed Nvf32-A1 and Nvf32-A2. The derived amino acid sequences of the Nvf32 proteins are highly homologous to the Vicia narbonensis ([alpha]/[beta])8-barrel seed protein narbonin. Considering this homology, Nvf32 is assumed to have a similar structure consisting of [beta]-sheets forming a central barrel surrounded by [alpha]-helices. The two Nvf32 sequences also contain two conserved amino acid motifs that are characteristic of class-III chitinases. Several amino acids demonstrated to be essential for chitinase activity are conserved in both regions, whereas one essential glutamic acid was changed to glycine in the Nvf32-A1 isoform but not in the Nvf32-A2 isoform.
