Viral Skin Disease

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The Experts below are selected from a list of 108 Experts worldwide ranked by ideXlab platform

Fei Hao - One of the best experts on this subject based on the ideXlab platform.

Yılmaz Akça - One of the best experts on this subject based on the ideXlab platform.

Qi-quan Chen - One of the best experts on this subject based on the ideXlab platform.

A. Mohammadi - One of the best experts on this subject based on the ideXlab platform.

  • Histopathological study and F1L gene sequence analysis of contagious ecthyma in small ruminants of Shiraz suburb, Iran.
    Tropical biomedicine, 2015
    Co-Authors: S. A. Davari, M. Sayyari, A. Mohammadi
    Abstract:

    Contagious ecthyma, also known as Orf, is a common Viral Skin Disease of sheep and goats caused by a Parapoxvirus. This research was conducted with the aims of histopathological study and genetic analysis of Orf virus with PCR technique based on F1L gene in 50 sheep and goats suspicious of contagious ecthyma in affected areas of Shiraz suburb. All 50 contagious ecthyma-like tissue samples were maintained in 10% buffered formalin, embedded in paraffin, sectioned into 5μm slices and dyed with hematoxylin-eosine. The histopathological examination showed 100% positivity. Epidermal hyperplasia with prominent rete ridges, hydropic degeneration of the necrotic keratinocytes, eosinophilic intracytoplasmic inclusion bodies in vacuolated cells and subcorneal pustules were the main hallmarks of this Disease. For molecular analysis, after DNA extraction, all samples were amplified by PCR method and the outcome demonstrated positivity in 25 specimens (50%). Of these, 10 definitely positive specimens were analyzed for nucleotide sequencing. Thus, a strain named Orf-059-Shiraz was recorded in the GeneBank and subsequently underwent phylogenetic analysis. The outcome of the molecular study approved half of the positive ecthyma specimens in histopathological method. Furthermore, phylogenetic analysis based on 059 gene showed that this gene is highly conserved. Utilization of histopathology and clinical signs can assist with rapid and low-cost diagnosis of infectious ecthyma whereas PCR is able to dissociate from similar Diseases among clinical samples of endemic regions.

  • Genetic analysis of the Viral agents causing muzzle crust in small ruminants of Shiraz, Iran.
    Bulgarian Journal of Veterinary Medicine, 2013
    Co-Authors: S. A. Davari, M. Sayyari, A. Mohammadi
    Abstract:

    Summary Davari, S. A., M. Sayyari & A. Mohammadi, 2013. Genetic analysis of the Viral agents causing muzzle crust in small ruminants of Shiraz, Iran. Bulg. J. Vet. Med., 16, No 3, 159−169. Orf virus (ORFV), the prototype of the genus parapoxvirus (PPV), is the cause of contagious ecthyma, a common Viral Skin Disease of domestic and wild small ruminants with a worldwide distribution. This Disease is endemic in Iran and it is sometimes difficult to differentiate it from similar Diseases, especially sheep pox, goat pox and peste des petits ruminants (PPR). In this study 50 muzzle crusts were collected from involved sheep and goats from Shiraz suburb during 2011–2012 and PCR was carried out for molecular detection. Twenty-five (50%) of 50 scab specimens were orf positive, 4 (8%) were pox virus positive and 1 (2%) was positive for PPRV. The PCR products were sequenced for nucleotide analysis. Furthermore, orf virus phylogenetic analysis based on 045 gene demonstrated a high degree of identity with other ORFV strains, although there was a slight diversity among the nucleotide sequences. Our study showed that the role of ORFV was greater than the other two viruses for causing muzzle crusts. This is the first molecular study of ORFV in Iran.

Zeynep Akkutay-yoldar - One of the best experts on this subject based on the ideXlab platform.