The Experts below are selected from a list of 6 Experts worldwide ranked by ideXlab platform
Michael M C Lai - One of the best experts on this subject based on the ideXlab platform.
-
molecular mechanism of hepatitis c Virus Carcinogenesis
2010Co-Authors: Keigo Machida, Michael M C LaiAbstract:Hepatitis C Virus (HCV) persistently infects 170 million people in the world and is a major cause of hepatocellular carcinoma (HCC). It can also induce B-cell proliferative disorders including B-cell lymphoma. HCV may induce cellular transformation by the induction of chronic liver inflammation mediated by the immune cells. This chronic liver inflammation leads to cell death, hepatocellular regeneration, and emergence of mutated cells that may be premalignant. In addition, HCV may also induce cellular transformation directly via the induction of oxidative stress and gene mutations as well as the alteration of cellular physiology, processes that may also lead to malignant cellular transformation. This chapter discusses the oncogenic
Keigo Machida - One of the best experts on this subject based on the ideXlab platform.
-
molecular mechanism of hepatitis c Virus Carcinogenesis
2010Co-Authors: Keigo Machida, Michael M C LaiAbstract:Hepatitis C Virus (HCV) persistently infects 170 million people in the world and is a major cause of hepatocellular carcinoma (HCC). It can also induce B-cell proliferative disorders including B-cell lymphoma. HCV may induce cellular transformation by the induction of chronic liver inflammation mediated by the immune cells. This chronic liver inflammation leads to cell death, hepatocellular regeneration, and emergence of mutated cells that may be premalignant. In addition, HCV may also induce cellular transformation directly via the induction of oxidative stress and gene mutations as well as the alteration of cellular physiology, processes that may also lead to malignant cellular transformation. This chapter discusses the oncogenic
Chen Jishen - One of the best experts on this subject based on the ideXlab platform.
-
the effect of hepatitis c Virus core gene transfection on expression of human telomerase reverse transcriptase mrna in uman biliary carcinoma cell lines
Chinese Journal of General Surgery, 2004Co-Authors: Chen JishenAbstract:Objective To study the effect of HCV C gene transfection on expression of hTERT mRNA in human biliary carcinoma cell lines (QBC939) and to elucidate the significance of activation of hTERT mRNA by HCV C gene on the Carcinogenesis of bile duct cells. MethodsThe recombinant plasmid(pcDNA3-HCVC) and the vector-alone were co-transfected with enhanced green fluorescent protein( EGFP )into QBC939 and human normal bile duct epithelial cells(HBEC) using liposome. The reverse transcription PCR(RT-PCR) and immunocytochemical stain were used to show the expression of hTERT mRNA and protein. Results The transfection efficiency of pcDNAHCV-C,which was determined by the expression of EGFP,is about 16% and 30% in QBC939 and HBEC respectively. There was no expression of hTERT mRNA assayed in HBECs when transfected blank vector,but a dramatic increase was observed for hTERT mRNA expression in HBEC when transfected with HCV C expressing vector. The expression of hTERT mRNA and protein assayed in QBC939 significantly increased when transfected with HCV C expression vector than that transfected with blank vector. Conclusion HCV C gene transfection up-regulates the transcriptional expression of hTERT mRNA in biliary carcinoma cells,it is suggested that HCV C protein contributes to Virus Carcinogenesis.
