The Experts below are selected from a list of 195 Experts worldwide ranked by ideXlab platform
Hans-w. Ackermann - One of the best experts on this subject based on the ideXlab platform.
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Taxonomy of bacterial Viruses: establishment of tailed Virus genera and the other Caudovirales
Archives of Virology, 1998Co-Authors: Jack Maniloff, Hans-w. AckermannAbstract:Bacterial Viruses have been classified into 13 families and 1 unassigned genus. A new order, Caudovirales , has now been established, comprising the three families of tailed bacterial Viruses, based on similarities in tailed Virus Morphology, replication, and assembly. In addition, genera have been established for some species in each tailed Virus family, based on properties involving viral DNA replication and packaging, and on some features specific to particular genera (e.g., DNA-termini linked proteins, Virus-encoded polymerases, and ability to establish temperate infections). At present, there are six genera in the family Myoviridae (Viruses with contractile tails), six in the family Siphoviridae (Viruses with long, noncontractile tails), and three in the family Podoviridae (Viruses with short noncontractile tails). In recognition that the definitions of tailed Virus genera represent a “work in progress” and to keep the nomenclature flexible, tailed Virus genera have been assigned vernacular names based on their type species.
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taxonomy of bacterial Viruses establishment of tailed Virus genera and the order caudovirales
Archives of Virology, 1998Co-Authors: Jack Maniloff, Hans-w. AckermannAbstract:Bacterial Viruses have been classified into 13 families and 1 unassigned genus. A new order, Caudovirales, has now been established, comprising the three families of tailed bacterial Viruses, based on similarities in tailed Virus Morphology, replication, and assembly. In addition, genera have been established for some species in each tailed Virus family, based on properties involving viral DNA replication and packaging, and on some features specific to particular genera (e.g., DNA-termini linked proteins, Virus-encoded polymerases, and ability to establish temperate infections). At present, there are six genera in the family Myoviridae (Viruses with contractile tails), six in the family Siphoviridae (Viruses with long, noncontractile tails), and three in the family Podoviridae (Viruses with short noncontractile tails). In recognition that the definitions of tailed Virus genera represent a “work in progress” and to keep the nomenclature flexible, tailed Virus genera have been assigned vernacular names based on their type species.
Jack Maniloff - One of the best experts on this subject based on the ideXlab platform.
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Taxonomy of bacterial Viruses: establishment of tailed Virus genera and the other Caudovirales
Archives of Virology, 1998Co-Authors: Jack Maniloff, Hans-w. AckermannAbstract:Bacterial Viruses have been classified into 13 families and 1 unassigned genus. A new order, Caudovirales , has now been established, comprising the three families of tailed bacterial Viruses, based on similarities in tailed Virus Morphology, replication, and assembly. In addition, genera have been established for some species in each tailed Virus family, based on properties involving viral DNA replication and packaging, and on some features specific to particular genera (e.g., DNA-termini linked proteins, Virus-encoded polymerases, and ability to establish temperate infections). At present, there are six genera in the family Myoviridae (Viruses with contractile tails), six in the family Siphoviridae (Viruses with long, noncontractile tails), and three in the family Podoviridae (Viruses with short noncontractile tails). In recognition that the definitions of tailed Virus genera represent a “work in progress” and to keep the nomenclature flexible, tailed Virus genera have been assigned vernacular names based on their type species.
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taxonomy of bacterial Viruses establishment of tailed Virus genera and the order caudovirales
Archives of Virology, 1998Co-Authors: Jack Maniloff, Hans-w. AckermannAbstract:Bacterial Viruses have been classified into 13 families and 1 unassigned genus. A new order, Caudovirales, has now been established, comprising the three families of tailed bacterial Viruses, based on similarities in tailed Virus Morphology, replication, and assembly. In addition, genera have been established for some species in each tailed Virus family, based on properties involving viral DNA replication and packaging, and on some features specific to particular genera (e.g., DNA-termini linked proteins, Virus-encoded polymerases, and ability to establish temperate infections). At present, there are six genera in the family Myoviridae (Viruses with contractile tails), six in the family Siphoviridae (Viruses with long, noncontractile tails), and three in the family Podoviridae (Viruses with short noncontractile tails). In recognition that the definitions of tailed Virus genera represent a “work in progress” and to keep the nomenclature flexible, tailed Virus genera have been assigned vernacular names based on their type species.
Andreas Nitsche - One of the best experts on this subject based on the ideXlab platform.
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Morphometry of SARS-CoV and SARS-CoV-2 particles in ultrathin plastic sections of infected Vero cell cultures
Scientific Reports, 2021Co-Authors: Michael Laue, Anne Kauter, Tobias Hoffmann, Lars Möller, Janine Michel, Andreas NitscheAbstract:SARS-CoV-2 is the causative of the COVID-19 disease, which has spread pandemically around the globe within a few months. It is therefore necessary to collect fundamental information about the disease, its epidemiology and treatment, as well as about the Virus itself. While the Virus has been identified rapidly, detailed ultrastructural analysis of Virus cell biology and architecture is still in its infancy. We therefore studied the Virus Morphology and morphometry of SARS-CoV-2 in comparison to SARS-CoV as it appears in Vero cell cultures by using conventional thin section electron microscopy and electron tomography. Both Virus isolates, SARS-CoV Frankfurt 1 and SARS-CoV-2 Italy-INMI1, were virtually identical at the ultrastructural level and revealed a very similar particle size distribution with a median of about 100 nm without spikes. Maximal spike length of both Viruses was 23 nm. The number of spikes per Virus particle was about 30% higher in the SARS-CoV than in the SARS-CoV-2 isolate. This result complements a previous qualitative finding, which was related to a lower productivity of SARS-CoV-2 in cell culture in comparison to SARS-CoV.
Michael Laue - One of the best experts on this subject based on the ideXlab platform.
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Morphometry of SARS-CoV and SARS-CoV-2 particles in ultrathin plastic sections of infected Vero cell cultures
Scientific Reports, 2021Co-Authors: Michael Laue, Anne Kauter, Tobias Hoffmann, Lars Möller, Janine Michel, Andreas NitscheAbstract:SARS-CoV-2 is the causative of the COVID-19 disease, which has spread pandemically around the globe within a few months. It is therefore necessary to collect fundamental information about the disease, its epidemiology and treatment, as well as about the Virus itself. While the Virus has been identified rapidly, detailed ultrastructural analysis of Virus cell biology and architecture is still in its infancy. We therefore studied the Virus Morphology and morphometry of SARS-CoV-2 in comparison to SARS-CoV as it appears in Vero cell cultures by using conventional thin section electron microscopy and electron tomography. Both Virus isolates, SARS-CoV Frankfurt 1 and SARS-CoV-2 Italy-INMI1, were virtually identical at the ultrastructural level and revealed a very similar particle size distribution with a median of about 100 nm without spikes. Maximal spike length of both Viruses was 23 nm. The number of spikes per Virus particle was about 30% higher in the SARS-CoV than in the SARS-CoV-2 isolate. This result complements a previous qualitative finding, which was related to a lower productivity of SARS-CoV-2 in cell culture in comparison to SARS-CoV.
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Electron Microscopy of Viruses
Methods in cell biology, 2010Co-Authors: Michael LaueAbstract:Electron microscopy is widely used in virology because Viruses are generally too small for a direct inspection by light microscopy. Analysis of Virus Morphology is necessary in many circumstances, e.g., for the diagnosis of a Virus in particular clinical situations or the analysis of Virus entry and assembly. Moreover, quality control of Virus particle integrity is required if a Virus is propagated in cell culture, particularly if the Virus genome has changed. In most cases already the basic methodology for transmission electron microscopy, i.e., negative staining and ultrathin sectioning, is sufficient to give relevant information on Virus ultrastructure. This chapter gives detailed information on the principles of these basic methodologies and provides simple but reliable protocols for a quick start. Moreover, the description of standard protocols for negative staining and ultrathin sectioning are supplemented by protocols on immuno-negative staining and rapid ultrathin sectioning. Finally, principles of methods for an extended ultrastructural research using more elaborate techniques, such as cryotechniques or methods to reveal the three-dimensional Virus architecture, are briefly reviewed.
Anne Kauter - One of the best experts on this subject based on the ideXlab platform.
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Morphometry of SARS-CoV and SARS-CoV-2 particles in ultrathin plastic sections of infected Vero cell cultures
Scientific Reports, 2021Co-Authors: Michael Laue, Anne Kauter, Tobias Hoffmann, Lars Möller, Janine Michel, Andreas NitscheAbstract:SARS-CoV-2 is the causative of the COVID-19 disease, which has spread pandemically around the globe within a few months. It is therefore necessary to collect fundamental information about the disease, its epidemiology and treatment, as well as about the Virus itself. While the Virus has been identified rapidly, detailed ultrastructural analysis of Virus cell biology and architecture is still in its infancy. We therefore studied the Virus Morphology and morphometry of SARS-CoV-2 in comparison to SARS-CoV as it appears in Vero cell cultures by using conventional thin section electron microscopy and electron tomography. Both Virus isolates, SARS-CoV Frankfurt 1 and SARS-CoV-2 Italy-INMI1, were virtually identical at the ultrastructural level and revealed a very similar particle size distribution with a median of about 100 nm without spikes. Maximal spike length of both Viruses was 23 nm. The number of spikes per Virus particle was about 30% higher in the SARS-CoV than in the SARS-CoV-2 isolate. This result complements a previous qualitative finding, which was related to a lower productivity of SARS-CoV-2 in cell culture in comparison to SARS-CoV.
