The Experts below are selected from a list of 225 Experts worldwide ranked by ideXlab platform
Heinz-peter Schultheiss - One of the best experts on this subject based on the ideXlab platform.
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Disturbance of myocardial energy metabolism in experimental Virus Myocarditis by antibodies against the adenine nucleotide translocator
Cardiovascular Research, 1999Co-Authors: Karsten Schulze, Bernhard Witzenbichler, Claudia Christmann, Heinz-peter SchultheissAbstract:Objective: The adenine nucleotide translocator (ANT) of the inner mitochondrial membrane is an autoantigen in Myocarditis and in dilated cardiomyopathy. Clinical and experimental studies showed that specific autoantibodies inhibit the transmembrane nucleotide transport. In isolated hearts of guinea pigs immunized with the ANT, energy metabolism is disturbed. This metabolic disorder is related to functionally active specific antibodies and to a reduced heart function. This study tests whether similar immunological, metabolical and functional responses also occur in experimental Virus Myocarditis. Methods and results: Experimental Virus Myocarditis was induced in A.SW/SnJ-mice by Coxsackie B3 Virus infection. Specific antibodies against the ANT were detected by Western Blot in 14 out of 19 infected animals. In the isolated perfused hearts of five of these 14 mice cytosolic and mitochondrial ATP/ADP-ratios, determined by nonaqueous fractionation, were significantly altered, signalling a reduced ANT function [cytosolic ATP/ADP: 59±18 vs. 136±20 (controls), mitochondrial ATP/ADP: 4.2±1.0 vs. 1.1±0.3], all P
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disturbance of myocardial energy metabolism in experimental Virus Myocarditis by antibodies against the adenine nucleotide translocator
Cardiovascular Research, 1999Co-Authors: Karsten Schulze, Bernhard Witzenbichler, Claudia Christmann, Heinz-peter SchultheissAbstract:Objective: The adenine nucleotide translocator (ANT) of the inner mitochondrial membrane is an autoantigen in Myocarditis and in dilated cardiomyopathy. Clinical and experimental studies showed that specific autoantibodies inhibit the transmembrane nucleotide transport. In isolated hearts of guinea pigs immunized with the ANT, energy metabolism is disturbed. This metabolic disorder is related to functionally active specific antibodies and to a reduced heart function. This study tests whether similar immunological, metabolical and functional responses also occur in experimental Virus Myocarditis. Methods and results: Experimental Virus Myocarditis was induced in A.SW/SnJ-mice by Coxsackie B3 Virus infection. Specific antibodies against the ANT were detected by Western Blot in 14 out of 19 infected animals. In the isolated perfused hearts of five of these 14 mice cytosolic and mitochondrial ATP/ADP-ratios, determined by nonaqueous fractionation, were significantly altered, signalling a reduced ANT function [cytosolic ATP/ADP: 59±18 vs. 136±20 (controls), mitochondrial ATP/ADP: 4.2±1.0 vs. 1.1±0.3], all P <0.05. Also, left ventricular pressure [43±9 vs. 78±6 mmHg (noninfected controls)], rate-pressure product (15.8±3.2 vs. 30.5±3.0 mmHg/min/1000), d p /d t (2410±222 vs. 3250±118 mmHg/s), and oxygen consumption (4.7±0.9 vs. 7.3±0.7 μmol/g/min), all P <0.05, were lowered. Conclusion: The data support the hypothesis that a Virus infection alters cardiac energy metabolism and function by an antibody-mediated modulation of the function of the ANT.
Hitoshi Maeda - One of the best experts on this subject based on the ideXlab platform.
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Epstein–Barr Virus Myocarditis as a cause of sudden death: two autopsy cases
International Journal of Legal Medicine, 2005Co-Authors: Takaki Ishikawa, Dong-ri Li, Dong Zhao, Hitoshi MaedaAbstract:Although the Epstein–Barr Virus (EBV) causes acute infection accompanied by a high fever in young people, there appears to be few reports of a fatal outcome involving Myocarditis. We report two cases of unexpected sudden death due to acute Myocarditis possibly caused by the EBV. They each visited a hospital due to common cold-like symptoms and unexpectedly died several days later. In both cases, autopsy revealed myocardial necrosis with marked lymphocytic infiltration. Polymerase chain reaction (PCR) screening was positive for the EBV, whereas immunohistochemistry and in situ hybridization for the EBV were negative. Serological investigations showed a mild elevation in antiviral capsid antigen IgG and anti-EBV nuclear antigen IgG in both cases. Immunohistochemical study of lymphocytic infiltrates showed strong positivity for a T-cell marker (CD45R_0) in the myocardium and pharyngeal mucosa. These cases suggest the potential risk of mortality from acute EBV infection in young people, even without severe clinical manifestations, and the importance of microbiological investigations, including PCR procedures, in postmortem diagnosis of infectious diseases.
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epstein barr Virus Myocarditis as a cause of sudden death two autopsy cases
International Journal of Legal Medicine, 2005Co-Authors: Takaki Ishikawa, Dong-ri Li, Dong Zhao, Hitoshi MaedaAbstract:Although the Epstein–Barr Virus (EBV) causes acute infection accompanied by a high fever in young people, there appears to be few reports of a fatal outcome involving Myocarditis. We report two cases of unexpected sudden death due to acute Myocarditis possibly caused by the EBV. They each visited a hospital due to common cold-like symptoms and unexpectedly died several days later. In both cases, autopsy revealed myocardial necrosis with marked lymphocytic infiltration. Polymerase chain reaction (PCR) screening was positive for the EBV, whereas immunohistochemistry and in situ hybridization for the EBV were negative. Serological investigations showed a mild elevation in antiviral capsid antigen IgG and anti-EBV nuclear antigen IgG in both cases. Immunohistochemical study of lymphocytic infiltrates showed strong positivity for a T-cell marker (CD45R0) in the myocardium and pharyngeal mucosa. These cases suggest the potential risk of mortality from acute EBV infection in young people, even without severe clinical manifestations, and the importance of microbiological investigations, including PCR procedures, in postmortem diagnosis of infectious diseases.
Karsten Schulze - One of the best experts on this subject based on the ideXlab platform.
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Disturbance of myocardial energy metabolism in experimental Virus Myocarditis by antibodies against the adenine nucleotide translocator
Cardiovascular Research, 1999Co-Authors: Karsten Schulze, Bernhard Witzenbichler, Claudia Christmann, Heinz-peter SchultheissAbstract:Objective: The adenine nucleotide translocator (ANT) of the inner mitochondrial membrane is an autoantigen in Myocarditis and in dilated cardiomyopathy. Clinical and experimental studies showed that specific autoantibodies inhibit the transmembrane nucleotide transport. In isolated hearts of guinea pigs immunized with the ANT, energy metabolism is disturbed. This metabolic disorder is related to functionally active specific antibodies and to a reduced heart function. This study tests whether similar immunological, metabolical and functional responses also occur in experimental Virus Myocarditis. Methods and results: Experimental Virus Myocarditis was induced in A.SW/SnJ-mice by Coxsackie B3 Virus infection. Specific antibodies against the ANT were detected by Western Blot in 14 out of 19 infected animals. In the isolated perfused hearts of five of these 14 mice cytosolic and mitochondrial ATP/ADP-ratios, determined by nonaqueous fractionation, were significantly altered, signalling a reduced ANT function [cytosolic ATP/ADP: 59±18 vs. 136±20 (controls), mitochondrial ATP/ADP: 4.2±1.0 vs. 1.1±0.3], all P
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disturbance of myocardial energy metabolism in experimental Virus Myocarditis by antibodies against the adenine nucleotide translocator
Cardiovascular Research, 1999Co-Authors: Karsten Schulze, Bernhard Witzenbichler, Claudia Christmann, Heinz-peter SchultheissAbstract:Objective: The adenine nucleotide translocator (ANT) of the inner mitochondrial membrane is an autoantigen in Myocarditis and in dilated cardiomyopathy. Clinical and experimental studies showed that specific autoantibodies inhibit the transmembrane nucleotide transport. In isolated hearts of guinea pigs immunized with the ANT, energy metabolism is disturbed. This metabolic disorder is related to functionally active specific antibodies and to a reduced heart function. This study tests whether similar immunological, metabolical and functional responses also occur in experimental Virus Myocarditis. Methods and results: Experimental Virus Myocarditis was induced in A.SW/SnJ-mice by Coxsackie B3 Virus infection. Specific antibodies against the ANT were detected by Western Blot in 14 out of 19 infected animals. In the isolated perfused hearts of five of these 14 mice cytosolic and mitochondrial ATP/ADP-ratios, determined by nonaqueous fractionation, were significantly altered, signalling a reduced ANT function [cytosolic ATP/ADP: 59±18 vs. 136±20 (controls), mitochondrial ATP/ADP: 4.2±1.0 vs. 1.1±0.3], all P <0.05. Also, left ventricular pressure [43±9 vs. 78±6 mmHg (noninfected controls)], rate-pressure product (15.8±3.2 vs. 30.5±3.0 mmHg/min/1000), d p /d t (2410±222 vs. 3250±118 mmHg/s), and oxygen consumption (4.7±0.9 vs. 7.3±0.7 μmol/g/min), all P <0.05, were lowered. Conclusion: The data support the hypothesis that a Virus infection alters cardiac energy metabolism and function by an antibody-mediated modulation of the function of the ANT.
Bezuhlova Sv - One of the best experts on this subject based on the ideXlab platform.
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Fermental (plasmin) blood system in patients with acute viral Myocarditis
Likars'ka sprava Ministerstvo okhorony zdorov'ia Ukraïny, 2009Co-Authors: Doroshenko Bh, Nazar Ps, Karpenko Oi, Kotko, Bezuhlova SvAbstract:: For the first time it is established that patients with acute Virus Myocarditis (AVM) at all stages of severity of clinical course of the disease have changes in indicators of fibrinogens, soluble complexes of monomeric fibrin, products of its degradation, activated plasminogen, time recalcification of blood plasma to blood heparin, fibrinolytic activity of plasma, spontaneous fibrinolysis, time of lysis of euglobulin clot, blood heparin, antithrombin III, platelets. It testifies to suppression of fibrinolysis processes which expressiveness statistically reliably correlates with degree of severity of clinical course of AVM. Application of medicamentous correction is necessary for prevention of the development of disseminated intravascular blood coagulation syndrome.
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State of physiological anticoagulant heparin, antithrombin III and their correction with medicines in patients with acute viral Myocarditis
Likars'ka sprava Ministerstvo okhorony zdorov'ia Ukraïny, 2008Co-Authors: Doroshenko Bh, Nazar Ps, Kotko Dm, Karpenko Oi, Bezuhlova SvAbstract:Abstract For the first time, positive influence was shown of a complex blood heparin-antitrombin III on the state of the coagulant and fibrinolytic system of blood in patients with acute Virus Myocarditis that favours the normalization of clinical and laboratory indicators, improvement of the state of patients and reduction of average bed-day indicator (hospital stay) till 25 and 35 days in accordance with severity of clinical course of the disease and can be recommended as a choice of medicine.
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Enzymatic (plasmin) blood system in patients with acute Virus Myocarditis and its medical correction
Likars'ka sprava Ministerstvo okhorony zdorov'ia Ukraïny, 2008Co-Authors: Doroshenko Bh, Nazar Ps, Kotko Dm, Karpenko Oi, Bezuhlova SvAbstract:: For the first time it is established that changes of parametres of coagulation hemostasis (fibrinogen Fg, soluble complexes of monomeasured fibrin -SCMF, products of fibrin degradation-PFD, 1st and 2nd waves of platelet aggregation), platelet hemostasis (platelets, a range index of platelet contact activation -RIPCA, activated partial platelet time--APPT, a total index thrombophilia TIT, platelet time--PT, prothrombin time (Prv), physiological anticoagulants (blood heparin-BH, antithrombin III--AT III, plasma tolerances to heparin--PTH), and fibrinolysis (lysis time of euglobulin clot--LTEC), enzyme condition of antiradical protection--superoxide scavenger (SOSC) which reduction in concentration leads to increase in concentration of malonic dialdehyde (MD) in erythrocytes (Er), thrombocytes (Tr), blood plasma (BP) in patients with acute Virus Myocarditis (AVM) of all severity groups and statistically correlate with the degree of severity of clinical course and demands medication correction.
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Coagulative hemostasis in patients with acute viral Myocarditis
Likars'ka sprava Ministerstvo okhorony zdorov'ia Ukraïny, 2008Co-Authors: Doroshenko Bh, Nazar Ps, Kotko Dm, Karpenko Oi, Bezuhlova SvAbstract:: For the first time it was established that changes of fibrinogen parameters, dissolved complexes of monomeric fibrinogen (DCMF), products of fibrinogen degradation (PDEF), first and second waves of platelet aggregation, activated partial platelet time (APPT), range index of contact platelet activation (RICPA), general index of thrombophilia (GIT), platelets, prothrombin time, heparin in blood, plasma tolerance to heparin (PTH), time lysis and euglobulin clot (TLEC), lipid peroxidation, antiradical defence ferment, superoxiddismutase (SOD) in patients with acute Virus Myocarditis of first, second and third group of severity correlate statistically reliably with grade of severity of clinical course of the disease and require correction with medications.
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Total index of thrombocyte aggregation in patients with an acute viral Myocarditis
Likars'ka sprava Ministerstvo okhorony zdorov'ia Ukraïny, 2007Co-Authors: Doroshenko Bh, Kotko Dm, Karpenko Oi, Bezuhlova SvAbstract:: For the first time it is proved, that patients with an acute Virus Myocarditis have statistically confirmed authentic reduction in quantity of thrombocytes, extention of parameters of a activated partial thrombin time, reduction in concentration of a heparin-antithrombin III (AT-III) complex against suppressed enzyme of antiradical protection - superoxide dismutase and it has led to activization of a malonic dialdehyde in erythrocytes, thrombocytes and blood serum. In case of the development of an acute Virus infection especially caused cardiotrophic strain Consaki, Virus CVB 1-CVB 4, thrombocytes, according to P. Nemetchek-Gansler, acquire not pertaining to them ability for phagocytosis of Viruses with following reduction in quantity of thrombocytes and increase in aggregation abilities of thrombocytes what influence the state of duration index of contact aggregation of thrombocytes with the tendency to shorten this duration, and also it may develop hypoxia, an ischemia and the subsequent necrosis of cardiomyocytes, that is to an acute Virus Myocarditis that demands corresponding correction with medications.
Doroshenko Bh - One of the best experts on this subject based on the ideXlab platform.
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Fermental (plasmin) blood system in patients with acute viral Myocarditis
Likars'ka sprava Ministerstvo okhorony zdorov'ia Ukraïny, 2009Co-Authors: Doroshenko Bh, Nazar Ps, Karpenko Oi, Kotko, Bezuhlova SvAbstract:: For the first time it is established that patients with acute Virus Myocarditis (AVM) at all stages of severity of clinical course of the disease have changes in indicators of fibrinogens, soluble complexes of monomeric fibrin, products of its degradation, activated plasminogen, time recalcification of blood plasma to blood heparin, fibrinolytic activity of plasma, spontaneous fibrinolysis, time of lysis of euglobulin clot, blood heparin, antithrombin III, platelets. It testifies to suppression of fibrinolysis processes which expressiveness statistically reliably correlates with degree of severity of clinical course of AVM. Application of medicamentous correction is necessary for prevention of the development of disseminated intravascular blood coagulation syndrome.
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State of physiological anticoagulant heparin, antithrombin III and their correction with medicines in patients with acute viral Myocarditis
Likars'ka sprava Ministerstvo okhorony zdorov'ia Ukraïny, 2008Co-Authors: Doroshenko Bh, Nazar Ps, Kotko Dm, Karpenko Oi, Bezuhlova SvAbstract:Abstract For the first time, positive influence was shown of a complex blood heparin-antitrombin III on the state of the coagulant and fibrinolytic system of blood in patients with acute Virus Myocarditis that favours the normalization of clinical and laboratory indicators, improvement of the state of patients and reduction of average bed-day indicator (hospital stay) till 25 and 35 days in accordance with severity of clinical course of the disease and can be recommended as a choice of medicine.
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Enzymatic (plasmin) blood system in patients with acute Virus Myocarditis and its medical correction
Likars'ka sprava Ministerstvo okhorony zdorov'ia Ukraïny, 2008Co-Authors: Doroshenko Bh, Nazar Ps, Kotko Dm, Karpenko Oi, Bezuhlova SvAbstract:: For the first time it is established that changes of parametres of coagulation hemostasis (fibrinogen Fg, soluble complexes of monomeasured fibrin -SCMF, products of fibrin degradation-PFD, 1st and 2nd waves of platelet aggregation), platelet hemostasis (platelets, a range index of platelet contact activation -RIPCA, activated partial platelet time--APPT, a total index thrombophilia TIT, platelet time--PT, prothrombin time (Prv), physiological anticoagulants (blood heparin-BH, antithrombin III--AT III, plasma tolerances to heparin--PTH), and fibrinolysis (lysis time of euglobulin clot--LTEC), enzyme condition of antiradical protection--superoxide scavenger (SOSC) which reduction in concentration leads to increase in concentration of malonic dialdehyde (MD) in erythrocytes (Er), thrombocytes (Tr), blood plasma (BP) in patients with acute Virus Myocarditis (AVM) of all severity groups and statistically correlate with the degree of severity of clinical course and demands medication correction.
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Coagulative hemostasis in patients with acute viral Myocarditis
Likars'ka sprava Ministerstvo okhorony zdorov'ia Ukraïny, 2008Co-Authors: Doroshenko Bh, Nazar Ps, Kotko Dm, Karpenko Oi, Bezuhlova SvAbstract:: For the first time it was established that changes of fibrinogen parameters, dissolved complexes of monomeric fibrinogen (DCMF), products of fibrinogen degradation (PDEF), first and second waves of platelet aggregation, activated partial platelet time (APPT), range index of contact platelet activation (RICPA), general index of thrombophilia (GIT), platelets, prothrombin time, heparin in blood, plasma tolerance to heparin (PTH), time lysis and euglobulin clot (TLEC), lipid peroxidation, antiradical defence ferment, superoxiddismutase (SOD) in patients with acute Virus Myocarditis of first, second and third group of severity correlate statistically reliably with grade of severity of clinical course of the disease and require correction with medications.
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Total index of thrombocyte aggregation in patients with an acute viral Myocarditis
Likars'ka sprava Ministerstvo okhorony zdorov'ia Ukraïny, 2007Co-Authors: Doroshenko Bh, Kotko Dm, Karpenko Oi, Bezuhlova SvAbstract:: For the first time it is proved, that patients with an acute Virus Myocarditis have statistically confirmed authentic reduction in quantity of thrombocytes, extention of parameters of a activated partial thrombin time, reduction in concentration of a heparin-antithrombin III (AT-III) complex against suppressed enzyme of antiradical protection - superoxide dismutase and it has led to activization of a malonic dialdehyde in erythrocytes, thrombocytes and blood serum. In case of the development of an acute Virus infection especially caused cardiotrophic strain Consaki, Virus CVB 1-CVB 4, thrombocytes, according to P. Nemetchek-Gansler, acquire not pertaining to them ability for phagocytosis of Viruses with following reduction in quantity of thrombocytes and increase in aggregation abilities of thrombocytes what influence the state of duration index of contact aggregation of thrombocytes with the tendency to shorten this duration, and also it may develop hypoxia, an ischemia and the subsequent necrosis of cardiomyocytes, that is to an acute Virus Myocarditis that demands corresponding correction with medications.
