Virus Resistance

14,000,000 Leading Edge Experts on the ideXlab platform

Scan Science and Technology

Contact Leading Edge Experts & Companies

Scan Science and Technology

Contact Leading Edge Experts & Companies

The Experts below are selected from a list of 91596 Experts worldwide ranked by ideXlab platform

Nam-hai Chua - One of the best experts on this subject based on the ideXlab platform.

  • Strategies and mechanisms of plant Virus Resistance
    Plant Biotechnology Reports, 2007
    Co-Authors: Rossana Henriques, Hui-wen Wu, Nam-hai Chua
    Abstract:

    Virus-induced diseases are responsible for major crop losses worldwide. A better understanding of plant defense mechanisms would lead to the development of novel strategies for effective plant protection. Early protein-based approaches relied mostly on the expression of transgenic coat protein (CP) to block the progression of the Virus infectious process. Other strategies exploit the plant’s innate defense mechanisms to combat invading viral pathogens. For example, the RNA-based Resistance makes use of the plant post-transcriptional gene silencing (PTGS) mechanism to degrade viral RNAs. In cross-protection the prior inoculation with a mild viral strain confers Resistance against a severe strain. Although the molecular detail of cross-protection is not fully understood, it is likely to be comprised of both protein- and RNA-based mechanisms, as well as some other unknown processes. In this review article we compare the benefits and challenges of these different viral-Resistance approaches. Furthermore, we discuss the development of a new approach based on the plant’s miRNA pathway. Artificial miRNAs with sequences complementary to viral sequences have been successfully used to generate Virus Resistance. This novel anti-viral strategy, which has the advantage of reducing possible bio-safety risks associated with protein- and RNA-based strategies, is a first step toward designing environmentally friendly Virus Resistance in transgenic crops.

  • Corrigendum: Expression of artificial microRNAs in transgenic Arabidopsis thaliana confers Virus Resistance
    Nature Biotechnology, 2007
    Co-Authors: Qi-wen Niu, Shih-shun Lin, José L. Reyes, Kuan-chun Chen, Shyi-dong Yeh, Nam-hai Chua
    Abstract:

    Corrigendum: Expression of artificial microRNAs in transgenic Arabidopsis thaliana confers Virus Resistance

  • expression of artificial micrornas in transgenic arabidopsis thaliana confers Virus Resistance
    Nature Biotechnology, 2006
    Co-Authors: José L. Reyes, Kuan-chun Chen, Hui-wen Wu, Nam-hai Chua
    Abstract:

    Plant microRNAs (miRNAs) regulate the abundance of target mRNAs by guiding their cleavage at the sequence complementary region. We have modified an Arabidopsis thaliana miR159 precursor to express artificial miRNAs (amiRNAs) targeting viral mRNA sequences encoding two gene silencing suppressors, P69 of turnip yellow mosaic Virus (TYMV) and HC-Pro of turnip mosaic Virus (TuMV). Production of these amiRNAs requires A. thaliana DICER-like protein 1. Transgenic A. thaliana plants expressing amiR-P69159 and amiR-HC-Pro159 are specifically resistant to TYMV and TuMV, respectively. Expression of amiR-TuCP159 targeting TuMV coat protein sequences also confers specific TuMV Resistance. However, transgenic plants that express both amiR-P69159 and amiR-HC-Pro159 from a dimeric pre-amiR-P69159/amiR-HC-Pro159 transgene are resistant to both Viruses. The Virus Resistance trait is displayed at the cell level and is hereditable. More important, the Resistance trait is maintained at 15 °C, a temperature that compromises small interfering RNA–mediated gene silencing. The amiRNA-mediated approach should have broad applicability for engineering multiple Virus Resistance in crop plants.

José L. Reyes - One of the best experts on this subject based on the ideXlab platform.

  • Corrigendum: Expression of artificial microRNAs in transgenic Arabidopsis thaliana confers Virus Resistance
    Nature Biotechnology, 2007
    Co-Authors: Qi-wen Niu, Shih-shun Lin, José L. Reyes, Kuan-chun Chen, Shyi-dong Yeh, Nam-hai Chua
    Abstract:

    Corrigendum: Expression of artificial microRNAs in transgenic Arabidopsis thaliana confers Virus Resistance

  • expression of artificial micrornas in transgenic arabidopsis thaliana confers Virus Resistance
    Nature Biotechnology, 2006
    Co-Authors: José L. Reyes, Kuan-chun Chen, Hui-wen Wu, Nam-hai Chua
    Abstract:

    Plant microRNAs (miRNAs) regulate the abundance of target mRNAs by guiding their cleavage at the sequence complementary region. We have modified an Arabidopsis thaliana miR159 precursor to express artificial miRNAs (amiRNAs) targeting viral mRNA sequences encoding two gene silencing suppressors, P69 of turnip yellow mosaic Virus (TYMV) and HC-Pro of turnip mosaic Virus (TuMV). Production of these amiRNAs requires A. thaliana DICER-like protein 1. Transgenic A. thaliana plants expressing amiR-P69159 and amiR-HC-Pro159 are specifically resistant to TYMV and TuMV, respectively. Expression of amiR-TuCP159 targeting TuMV coat protein sequences also confers specific TuMV Resistance. However, transgenic plants that express both amiR-P69159 and amiR-HC-Pro159 from a dimeric pre-amiR-P69159/amiR-HC-Pro159 transgene are resistant to both Viruses. The Virus Resistance trait is displayed at the cell level and is hereditable. More important, the Resistance trait is maintained at 15 °C, a temperature that compromises small interfering RNA–mediated gene silencing. The amiRNA-mediated approach should have broad applicability for engineering multiple Virus Resistance in crop plants.

Kuan-chun Chen - One of the best experts on this subject based on the ideXlab platform.

  • Corrigendum: Expression of artificial microRNAs in transgenic Arabidopsis thaliana confers Virus Resistance
    Nature Biotechnology, 2007
    Co-Authors: Qi-wen Niu, Shih-shun Lin, José L. Reyes, Kuan-chun Chen, Shyi-dong Yeh, Nam-hai Chua
    Abstract:

    Corrigendum: Expression of artificial microRNAs in transgenic Arabidopsis thaliana confers Virus Resistance

  • expression of artificial micrornas in transgenic arabidopsis thaliana confers Virus Resistance
    Nature Biotechnology, 2006
    Co-Authors: José L. Reyes, Kuan-chun Chen, Hui-wen Wu, Nam-hai Chua
    Abstract:

    Plant microRNAs (miRNAs) regulate the abundance of target mRNAs by guiding their cleavage at the sequence complementary region. We have modified an Arabidopsis thaliana miR159 precursor to express artificial miRNAs (amiRNAs) targeting viral mRNA sequences encoding two gene silencing suppressors, P69 of turnip yellow mosaic Virus (TYMV) and HC-Pro of turnip mosaic Virus (TuMV). Production of these amiRNAs requires A. thaliana DICER-like protein 1. Transgenic A. thaliana plants expressing amiR-P69159 and amiR-HC-Pro159 are specifically resistant to TYMV and TuMV, respectively. Expression of amiR-TuCP159 targeting TuMV coat protein sequences also confers specific TuMV Resistance. However, transgenic plants that express both amiR-P69159 and amiR-HC-Pro159 from a dimeric pre-amiR-P69159/amiR-HC-Pro159 transgene are resistant to both Viruses. The Virus Resistance trait is displayed at the cell level and is hereditable. More important, the Resistance trait is maintained at 15 °C, a temperature that compromises small interfering RNA–mediated gene silencing. The amiRNA-mediated approach should have broad applicability for engineering multiple Virus Resistance in crop plants.

Gabriele Krczal - One of the best experts on this subject based on the ideXlab platform.

  • Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer Virus Resistance
    Nature Biotechnology, 2004
    Co-Authors: Kajohn Boonrod, Danuta Galetzka, Udo Conrad, Peter D Nagy, Gabriele Krczal
    Abstract:

    Crop loss due to viral diseases is still a major problem for agriculture today. We present a strategy to achieve Virus Resistance based on the expression of single-chain Fv fragments (scFvs) against a conserved domain in a plant viral RNA-dependent RNA polymerase (RdRp), a key enzyme in Virus replication. The selected scFvs inhibited complementary RNA synthesis of different plant Virus RdRps in vitro and Virus replication in planta . Moreover, the scFvs also bound to the RdRp of the distantly related hepatitis C Virus. T_1 and T_2 progeny of transgenic lines of Nicotiana benthamiana expressing different scFvs either in the cytosol or in the endoplasmic reticulum showed varying degrees of Resistance against four plant Viruses from different genera, three of which belong to the Tombusviridae family. Virus Resistance based on antibodies to RdRps adds another tool to the repertoire for combating plant Viruses.

  • single chain antibodies against a plant viral rna dependent rna polymerase confer Virus Resistance
    Nature Biotechnology, 2004
    Co-Authors: Kajohn Boonrod, Danuta Galetzka, Udo Conrad, Peter D Nagy, Gabriele Krczal
    Abstract:

    Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer Virus Resistance

Kajohn Boonrod - One of the best experts on this subject based on the ideXlab platform.

  • Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer Virus Resistance
    Nature Biotechnology, 2004
    Co-Authors: Kajohn Boonrod, Danuta Galetzka, Udo Conrad, Peter D Nagy, Gabriele Krczal
    Abstract:

    Crop loss due to viral diseases is still a major problem for agriculture today. We present a strategy to achieve Virus Resistance based on the expression of single-chain Fv fragments (scFvs) against a conserved domain in a plant viral RNA-dependent RNA polymerase (RdRp), a key enzyme in Virus replication. The selected scFvs inhibited complementary RNA synthesis of different plant Virus RdRps in vitro and Virus replication in planta . Moreover, the scFvs also bound to the RdRp of the distantly related hepatitis C Virus. T_1 and T_2 progeny of transgenic lines of Nicotiana benthamiana expressing different scFvs either in the cytosol or in the endoplasmic reticulum showed varying degrees of Resistance against four plant Viruses from different genera, three of which belong to the Tombusviridae family. Virus Resistance based on antibodies to RdRps adds another tool to the repertoire for combating plant Viruses.

  • single chain antibodies against a plant viral rna dependent rna polymerase confer Virus Resistance
    Nature Biotechnology, 2004
    Co-Authors: Kajohn Boonrod, Danuta Galetzka, Udo Conrad, Peter D Nagy, Gabriele Krczal
    Abstract:

    Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer Virus Resistance

Related terms

Virus Resistance - 15 days free trial to Access Experts & Abstracts