The Experts below are selected from a list of 24945 Experts worldwide ranked by ideXlab platform
Alois Jungbauer - One of the best experts on this subject based on the ideXlab platform.
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generation of human monoclonal antibodies against hiv 1 proteins electrofusion and epstein barr Virus Transformation for peripheral blood lymphocyte immortalization
AIDS Research and Human Retroviruses, 1994Co-Authors: Andrea Buchacher, Renate Predl, K Strutzenberger, Willibald Steinfellner, A Trkola, Martin Purtscher, Gerhard Gruber, Christa Tauer, Franz Steindl, Alois JungbauerAbstract:Electrofusion and EBV Transformation were studied by immortalizing human PBLs from blood of HIV-1-positive volunteers. A panel of 33 cell lines producing human monoclonal antibodies (Hu-MAbs) against HIV-1 was established by cell fusion or EBV Transformation. For the first fusion experiments the source of B lymphocytes was peripheral blood of HIV-1-infected donors in CDC stages II or III with CD4 cell counts higher than 500/mm3. Later on, from these patients only, those with high anti-HIV titers were chosen as blood donors. By that means the yield of stable specific hybridomas was increased twofold. In our experiments electrofusion turned out to be a more efficient immortalization method than EBV Transformation, due to a high and constant immortalization rate. The hybridomas were stable after intensive subcloning and could be cultivated over a period of 8 months without loss in monoclonal antibody production. Immunoglobulin class, subtype, reactivity against HIV-1 proteins, Western blot patterns, immunofl...
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generation of human monoclonal antibodies against hiv 1 proteins electrofusion and epstein barr Virus Transformation for peripheral blood lymphocyte immortalization
AIDS Research and Human Retroviruses, 1994Co-Authors: Andrea Buchacher, Renate Predl, K Strutzenberger, Willibald Steinfellner, A Trkola, Martin Purtscher, Gerhard Gruber, Christa Tauer, Franz Steindl, Alois JungbauerAbstract:Electrofusion and EBV Transformation were studied by immortalizing human PBLs from blood of HIV-1-positive volunteers. A panel of 33 cell lines producing human monoclonal antibodies (Hu-MAbs) against HIV-1 was established by cell fusion or EBV Transformation. For the first fusion experiments the source of B lymphocytes was peripheral blood of HIV-1-infected donors in CDC stages II or III with CD4 cell counts higher than 500/mm3. Later on, from these patients only, those with high anti-HIV titers were chosen as blood donors. By that means the yield of stable specific hybridomas was increased twofold. In our experiments electrofusion turned out to be a more efficient immortalization method than EBV Transformation, due to a high and constant immortalization rate. The hybridomas were stable after intensive subcloning and could be cultivated over a period of 8 months without loss in monoclonal antibody production. Immunoglobulin class, subtype, reactivity against HIV-1 proteins, Western blot patterns, immunofl...
Andrea Buchacher - One of the best experts on this subject based on the ideXlab platform.
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generation of human monoclonal antibodies against hiv 1 proteins electrofusion and epstein barr Virus Transformation for peripheral blood lymphocyte immortalization
AIDS Research and Human Retroviruses, 1994Co-Authors: Andrea Buchacher, Renate Predl, K Strutzenberger, Willibald Steinfellner, A Trkola, Martin Purtscher, Gerhard Gruber, Christa Tauer, Franz Steindl, Alois JungbauerAbstract:Electrofusion and EBV Transformation were studied by immortalizing human PBLs from blood of HIV-1-positive volunteers. A panel of 33 cell lines producing human monoclonal antibodies (Hu-MAbs) against HIV-1 was established by cell fusion or EBV Transformation. For the first fusion experiments the source of B lymphocytes was peripheral blood of HIV-1-infected donors in CDC stages II or III with CD4 cell counts higher than 500/mm3. Later on, from these patients only, those with high anti-HIV titers were chosen as blood donors. By that means the yield of stable specific hybridomas was increased twofold. In our experiments electrofusion turned out to be a more efficient immortalization method than EBV Transformation, due to a high and constant immortalization rate. The hybridomas were stable after intensive subcloning and could be cultivated over a period of 8 months without loss in monoclonal antibody production. Immunoglobulin class, subtype, reactivity against HIV-1 proteins, Western blot patterns, immunofl...
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generation of human monoclonal antibodies against hiv 1 proteins electrofusion and epstein barr Virus Transformation for peripheral blood lymphocyte immortalization
AIDS Research and Human Retroviruses, 1994Co-Authors: Andrea Buchacher, Renate Predl, K Strutzenberger, Willibald Steinfellner, A Trkola, Martin Purtscher, Gerhard Gruber, Christa Tauer, Franz Steindl, Alois JungbauerAbstract:Electrofusion and EBV Transformation were studied by immortalizing human PBLs from blood of HIV-1-positive volunteers. A panel of 33 cell lines producing human monoclonal antibodies (Hu-MAbs) against HIV-1 was established by cell fusion or EBV Transformation. For the first fusion experiments the source of B lymphocytes was peripheral blood of HIV-1-infected donors in CDC stages II or III with CD4 cell counts higher than 500/mm3. Later on, from these patients only, those with high anti-HIV titers were chosen as blood donors. By that means the yield of stable specific hybridomas was increased twofold. In our experiments electrofusion turned out to be a more efficient immortalization method than EBV Transformation, due to a high and constant immortalization rate. The hybridomas were stable after intensive subcloning and could be cultivated over a period of 8 months without loss in monoclonal antibody production. Immunoglobulin class, subtype, reactivity against HIV-1 proteins, Western blot patterns, immunofl...
Willibald Steinfellner - One of the best experts on this subject based on the ideXlab platform.
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generation of human monoclonal antibodies against hiv 1 proteins electrofusion and epstein barr Virus Transformation for peripheral blood lymphocyte immortalization
AIDS Research and Human Retroviruses, 1994Co-Authors: Andrea Buchacher, Renate Predl, K Strutzenberger, Willibald Steinfellner, A Trkola, Martin Purtscher, Gerhard Gruber, Christa Tauer, Franz Steindl, Alois JungbauerAbstract:Electrofusion and EBV Transformation were studied by immortalizing human PBLs from blood of HIV-1-positive volunteers. A panel of 33 cell lines producing human monoclonal antibodies (Hu-MAbs) against HIV-1 was established by cell fusion or EBV Transformation. For the first fusion experiments the source of B lymphocytes was peripheral blood of HIV-1-infected donors in CDC stages II or III with CD4 cell counts higher than 500/mm3. Later on, from these patients only, those with high anti-HIV titers were chosen as blood donors. By that means the yield of stable specific hybridomas was increased twofold. In our experiments electrofusion turned out to be a more efficient immortalization method than EBV Transformation, due to a high and constant immortalization rate. The hybridomas were stable after intensive subcloning and could be cultivated over a period of 8 months without loss in monoclonal antibody production. Immunoglobulin class, subtype, reactivity against HIV-1 proteins, Western blot patterns, immunofl...
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generation of human monoclonal antibodies against hiv 1 proteins electrofusion and epstein barr Virus Transformation for peripheral blood lymphocyte immortalization
AIDS Research and Human Retroviruses, 1994Co-Authors: Andrea Buchacher, Renate Predl, K Strutzenberger, Willibald Steinfellner, A Trkola, Martin Purtscher, Gerhard Gruber, Christa Tauer, Franz Steindl, Alois JungbauerAbstract:Electrofusion and EBV Transformation were studied by immortalizing human PBLs from blood of HIV-1-positive volunteers. A panel of 33 cell lines producing human monoclonal antibodies (Hu-MAbs) against HIV-1 was established by cell fusion or EBV Transformation. For the first fusion experiments the source of B lymphocytes was peripheral blood of HIV-1-infected donors in CDC stages II or III with CD4 cell counts higher than 500/mm3. Later on, from these patients only, those with high anti-HIV titers were chosen as blood donors. By that means the yield of stable specific hybridomas was increased twofold. In our experiments electrofusion turned out to be a more efficient immortalization method than EBV Transformation, due to a high and constant immortalization rate. The hybridomas were stable after intensive subcloning and could be cultivated over a period of 8 months without loss in monoclonal antibody production. Immunoglobulin class, subtype, reactivity against HIV-1 proteins, Western blot patterns, immunofl...
Gerhard Gruber - One of the best experts on this subject based on the ideXlab platform.
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generation of human monoclonal antibodies against hiv 1 proteins electrofusion and epstein barr Virus Transformation for peripheral blood lymphocyte immortalization
AIDS Research and Human Retroviruses, 1994Co-Authors: Andrea Buchacher, Renate Predl, K Strutzenberger, Willibald Steinfellner, A Trkola, Martin Purtscher, Gerhard Gruber, Christa Tauer, Franz Steindl, Alois JungbauerAbstract:Electrofusion and EBV Transformation were studied by immortalizing human PBLs from blood of HIV-1-positive volunteers. A panel of 33 cell lines producing human monoclonal antibodies (Hu-MAbs) against HIV-1 was established by cell fusion or EBV Transformation. For the first fusion experiments the source of B lymphocytes was peripheral blood of HIV-1-infected donors in CDC stages II or III with CD4 cell counts higher than 500/mm3. Later on, from these patients only, those with high anti-HIV titers were chosen as blood donors. By that means the yield of stable specific hybridomas was increased twofold. In our experiments electrofusion turned out to be a more efficient immortalization method than EBV Transformation, due to a high and constant immortalization rate. The hybridomas were stable after intensive subcloning and could be cultivated over a period of 8 months without loss in monoclonal antibody production. Immunoglobulin class, subtype, reactivity against HIV-1 proteins, Western blot patterns, immunofl...
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generation of human monoclonal antibodies against hiv 1 proteins electrofusion and epstein barr Virus Transformation for peripheral blood lymphocyte immortalization
AIDS Research and Human Retroviruses, 1994Co-Authors: Andrea Buchacher, Renate Predl, K Strutzenberger, Willibald Steinfellner, A Trkola, Martin Purtscher, Gerhard Gruber, Christa Tauer, Franz Steindl, Alois JungbauerAbstract:Electrofusion and EBV Transformation were studied by immortalizing human PBLs from blood of HIV-1-positive volunteers. A panel of 33 cell lines producing human monoclonal antibodies (Hu-MAbs) against HIV-1 was established by cell fusion or EBV Transformation. For the first fusion experiments the source of B lymphocytes was peripheral blood of HIV-1-infected donors in CDC stages II or III with CD4 cell counts higher than 500/mm3. Later on, from these patients only, those with high anti-HIV titers were chosen as blood donors. By that means the yield of stable specific hybridomas was increased twofold. In our experiments electrofusion turned out to be a more efficient immortalization method than EBV Transformation, due to a high and constant immortalization rate. The hybridomas were stable after intensive subcloning and could be cultivated over a period of 8 months without loss in monoclonal antibody production. Immunoglobulin class, subtype, reactivity against HIV-1 proteins, Western blot patterns, immunofl...
Renate Predl - One of the best experts on this subject based on the ideXlab platform.
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generation of human monoclonal antibodies against hiv 1 proteins electrofusion and epstein barr Virus Transformation for peripheral blood lymphocyte immortalization
AIDS Research and Human Retroviruses, 1994Co-Authors: Andrea Buchacher, Renate Predl, K Strutzenberger, Willibald Steinfellner, A Trkola, Martin Purtscher, Gerhard Gruber, Christa Tauer, Franz Steindl, Alois JungbauerAbstract:Electrofusion and EBV Transformation were studied by immortalizing human PBLs from blood of HIV-1-positive volunteers. A panel of 33 cell lines producing human monoclonal antibodies (Hu-MAbs) against HIV-1 was established by cell fusion or EBV Transformation. For the first fusion experiments the source of B lymphocytes was peripheral blood of HIV-1-infected donors in CDC stages II or III with CD4 cell counts higher than 500/mm3. Later on, from these patients only, those with high anti-HIV titers were chosen as blood donors. By that means the yield of stable specific hybridomas was increased twofold. In our experiments electrofusion turned out to be a more efficient immortalization method than EBV Transformation, due to a high and constant immortalization rate. The hybridomas were stable after intensive subcloning and could be cultivated over a period of 8 months without loss in monoclonal antibody production. Immunoglobulin class, subtype, reactivity against HIV-1 proteins, Western blot patterns, immunofl...
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generation of human monoclonal antibodies against hiv 1 proteins electrofusion and epstein barr Virus Transformation for peripheral blood lymphocyte immortalization
AIDS Research and Human Retroviruses, 1994Co-Authors: Andrea Buchacher, Renate Predl, K Strutzenberger, Willibald Steinfellner, A Trkola, Martin Purtscher, Gerhard Gruber, Christa Tauer, Franz Steindl, Alois JungbauerAbstract:Electrofusion and EBV Transformation were studied by immortalizing human PBLs from blood of HIV-1-positive volunteers. A panel of 33 cell lines producing human monoclonal antibodies (Hu-MAbs) against HIV-1 was established by cell fusion or EBV Transformation. For the first fusion experiments the source of B lymphocytes was peripheral blood of HIV-1-infected donors in CDC stages II or III with CD4 cell counts higher than 500/mm3. Later on, from these patients only, those with high anti-HIV titers were chosen as blood donors. By that means the yield of stable specific hybridomas was increased twofold. In our experiments electrofusion turned out to be a more efficient immortalization method than EBV Transformation, due to a high and constant immortalization rate. The hybridomas were stable after intensive subcloning and could be cultivated over a period of 8 months without loss in monoclonal antibody production. Immunoglobulin class, subtype, reactivity against HIV-1 proteins, Western blot patterns, immunofl...
