The Experts below are selected from a list of 126 Experts worldwide ranked by ideXlab platform
Jens Nielsen - One of the best experts on this subject based on the ideXlab platform.
-
metagenomic analysis of microbe mediated Vitamin Metabolism in the human gut microbiome
BMC Genomics, 2019Co-Authors: Promi Das, Parizad Babaei, Jens NielsenAbstract:Human gut microbial communities have been known to produce Vitamins, which are subsequently absorbed by the host in the large intestine. However, the relationship between species with Vitamin pathway associated functional features or their gene abundance in different states of health and disease is lacking. Here, we analyzed shotgun fecal metagenomes of individuals from four different countries for genes that are involved in Vitamin biosynthetic pathways and transport mechanisms and corresponding species’ abundance. We found that the prevalence of these genes were found to be distributed across the dominant phyla of gut species. The number of positive correlations were high between species harboring genes related to Vitamin biosynthetic pathways and transporter mechanisms than that with either alone. Although, the range of total gene abundances remained constant across healthy populations at the global level, species composition and their presence for metabolic pathway related genes determine the abundance and functional genetic content of Vitamin Metabolism. Based on metatranscriptomics data, the equation between abundance of Vitamin-biosynthetic enzymes and Vitamin-dependent enzymes suggests that the production and utilization potential of these enzymes seems way more complex usage allocations than just mere direct linear associations. Our findings provide a rationale to examine and disentangle the interrelationship between B-Vitamin dosage (dietary or microbe-mediated) on gut microbial members and the host, in the gut microbiota of individuals with under- or overnutrition.
-
Additional file 4: of Metagenomic analysis of microbe-mediated Vitamin Metabolism in the human gut microbiome
2019Co-Authors: Promi Das, Parizad Babaei, Jens NielsenAbstract:Figure S3. Alluvial plot showing the relationship between the kind of correlation for each Vitamin type (biotin, cobalamin and thiamine) and the possible phenotype combinations. (DOCX 3751 kb
Promi Das - One of the best experts on this subject based on the ideXlab platform.
-
metagenomic analysis of microbe mediated Vitamin Metabolism in the human gut microbiome
BMC Genomics, 2019Co-Authors: Promi Das, Parizad Babaei, Jens NielsenAbstract:Human gut microbial communities have been known to produce Vitamins, which are subsequently absorbed by the host in the large intestine. However, the relationship between species with Vitamin pathway associated functional features or their gene abundance in different states of health and disease is lacking. Here, we analyzed shotgun fecal metagenomes of individuals from four different countries for genes that are involved in Vitamin biosynthetic pathways and transport mechanisms and corresponding species’ abundance. We found that the prevalence of these genes were found to be distributed across the dominant phyla of gut species. The number of positive correlations were high between species harboring genes related to Vitamin biosynthetic pathways and transporter mechanisms than that with either alone. Although, the range of total gene abundances remained constant across healthy populations at the global level, species composition and their presence for metabolic pathway related genes determine the abundance and functional genetic content of Vitamin Metabolism. Based on metatranscriptomics data, the equation between abundance of Vitamin-biosynthetic enzymes and Vitamin-dependent enzymes suggests that the production and utilization potential of these enzymes seems way more complex usage allocations than just mere direct linear associations. Our findings provide a rationale to examine and disentangle the interrelationship between B-Vitamin dosage (dietary or microbe-mediated) on gut microbial members and the host, in the gut microbiota of individuals with under- or overnutrition.
-
Additional file 4: of Metagenomic analysis of microbe-mediated Vitamin Metabolism in the human gut microbiome
2019Co-Authors: Promi Das, Parizad Babaei, Jens NielsenAbstract:Figure S3. Alluvial plot showing the relationship between the kind of correlation for each Vitamin type (biotin, cobalamin and thiamine) and the possible phenotype combinations. (DOCX 3751 kb
Rajesh Dabur - One of the best experts on this subject based on the ideXlab platform.
-
intervention of ayurvedic drug tinospora cordifolia attenuates the metabolic alterations in hypertriglyceridemia a pilot clinical trial
Journal of diabetes and metabolic disorders, 2020Co-Authors: Amey Shirolkar, Aarti Yadav, T. K. Mandal, Rajesh DaburAbstract:Purpose Hypertriglyceridemia (HG) is an independent risk factor with more prevalence than hypercholesterolemia and its attributes to cardiovascular disease (CVD) and pancreatitis. Hence, it becomes imperative to search for new triglyceride (TG) lowering agents. Tinospora cordifolia (TC) is a well-known Ayurvedic drug and a rich source of protoberberine alkaloids hence can contribute to TG lowering without side effects. Hence, to explore the therapeutic efficacy of T. cordifolia and its effects on biochemistry and metabolome in the patients of hyper-triglyceridemia, clinical trials were conducted. Methods Patients (n = 24) with hypertriglyceridemia were randomized into two groups to receive T. cordifolia extract (TCE) (3.0 g/per day) and metformin (850 mg/day) for 14 days having >300 mg/dl triglyceride level and cholesterol in the range of 130-230 mg/dl. Lipid profiles of blood samples were analyzed. Urine samples were subjected to HPLC-QTOF-MS to quantify oxidative damage and abnormal metabolic regulation. Results Intervention with TCE reduced the triglyceride, LDL, and VLDL levels to 380.45 ± 17.44, 133.25 ± 3.18, and 31.85 ± 5.88 mg/dL and increased the HDL to 47.50 ± 9.05 mg/dL significantly (p < 0.05) in the HG patients after 14 days treatment. TCE dosage potently suppressed the inflammatory and oxidative stress marker's i.e. levels of isoprostanes significantly (p < 0.01). Qualitative metabolomics approach i.e. PCA and PLS-DA showed significant alterations (p < 0.05) in the levels of 40 metabolites in the urine samples from different groups. Conclusion TCE administration depleted the levels of markers of HG i.e. VLDL, TG, and LDL significantly. Metabolomics studies established that the anti-HG activity of TCE was due to its antioxidative potential and modulation of the biopterin, butanoate, amino acid, and Vitamin Metabolism. Clinical trials registry India (CTRI) registration no. CTRI- 2016-08-007187.
-
protective effects of tinospora cordifolia on hepatic and gastrointestinal toxicity induced by chronic and moderate alcoholism
Alcohol and Alcoholism, 2016Co-Authors: Bhawana Sharma, Rajesh DaburAbstract:Aims Heavy alcohol intake depletes the plasma Vitamins due to hepatotoxicity and decreased intestinal absorption. However, moderate alcohol intake is often thought to be healthy. Therefore, effects of chronic moderate alcohol intake on liver and intestine were studied using urinary Vitamin levels. Furthermore, effects of Tinospora cordifolia water extract (TCE) (hepatoprotective) on Vitamin excretion and intestinal absorption were also studied. Methods In the study, asymptomatic moderate alcoholics ( n = 12) without chronic liver disease and healthy volunteers ( n = 14) of mean age 39 ± 2.2 (mean ± SD) were selected and divided into three groups. TCE treatment was performed for 14 days. The blood and urine samples were collected on Day 0 and 14 after treatment with TCE and analyzed. Results In alcoholics samples, a significant increase in the levels of gamma-glutamyl transferase, aspartate transaminase, alanine transaminase, Triglyceride, Cholesterol, HDL and LDL ( P < 0.05) was observed but their level get downregulated after TCE intervention. Multivariate analysis of metabolites without missing values showed an increased excretion of 7-dehydrocholesterol, orotic acid, pyridoxine, lipoamide and niacin and TCE intervention depleted their levels ( P < 0.05). In contrast, excretion of biotin, xanthine, Vitamin D2 and 2- O - p -coumaroyltartronic acid (CA, an internal marker of intestinal absorption) were observed to be decreased in alcoholic samples; however, TCE intervention restored the CA and biotin levels. Vitamin Metabolism biomarkers, i.e. homocysteine and xanthurenic acid, were also normalized after TCE intervention. Conclusion Overall data depict that moderate alcohol intake is also hepatotoxic and decreases intestinal absorption. However, TCE treatment effectively increased the intestinal absorption and retaining power of liver that regulated alcohol-induced multiVitamin deficiency.
Parizad Babaei - One of the best experts on this subject based on the ideXlab platform.
-
metagenomic analysis of microbe mediated Vitamin Metabolism in the human gut microbiome
BMC Genomics, 2019Co-Authors: Promi Das, Parizad Babaei, Jens NielsenAbstract:Human gut microbial communities have been known to produce Vitamins, which are subsequently absorbed by the host in the large intestine. However, the relationship between species with Vitamin pathway associated functional features or their gene abundance in different states of health and disease is lacking. Here, we analyzed shotgun fecal metagenomes of individuals from four different countries for genes that are involved in Vitamin biosynthetic pathways and transport mechanisms and corresponding species’ abundance. We found that the prevalence of these genes were found to be distributed across the dominant phyla of gut species. The number of positive correlations were high between species harboring genes related to Vitamin biosynthetic pathways and transporter mechanisms than that with either alone. Although, the range of total gene abundances remained constant across healthy populations at the global level, species composition and their presence for metabolic pathway related genes determine the abundance and functional genetic content of Vitamin Metabolism. Based on metatranscriptomics data, the equation between abundance of Vitamin-biosynthetic enzymes and Vitamin-dependent enzymes suggests that the production and utilization potential of these enzymes seems way more complex usage allocations than just mere direct linear associations. Our findings provide a rationale to examine and disentangle the interrelationship between B-Vitamin dosage (dietary or microbe-mediated) on gut microbial members and the host, in the gut microbiota of individuals with under- or overnutrition.
-
Additional file 4: of Metagenomic analysis of microbe-mediated Vitamin Metabolism in the human gut microbiome
2019Co-Authors: Promi Das, Parizad Babaei, Jens NielsenAbstract:Figure S3. Alluvial plot showing the relationship between the kind of correlation for each Vitamin type (biotin, cobalamin and thiamine) and the possible phenotype combinations. (DOCX 3751 kb
Melissa J. Remis - One of the best experts on this subject based on the ideXlab platform.
-
gut microbiome of coexisting baaka pygmies and bantu reflects gradients of traditional subsistence patterns
Cell Reports, 2016Co-Authors: Andres Gomez, Klara J. Petrzelkova, Klara Vlckova, Angelique Todd, David Modrý, Carl J Yeoman, Katherine R Amato, Michael B Burns, Carolyn Jost A Robinson, Melissa J. RemisAbstract:To understand how the gut microbiome is impacted by human adaptation to varying environments, we explored gut bacterial communities in the BaAka rainforest hunter-gatherers and their agriculturalist Bantu neighbors in the Central African Republic. Although the microbiome of both groups is compositionally similar, hunter-gatherers harbor increased abundance of Prevotellaceae, Treponema, and Clostridiaceae, while the Bantu gut microbiome is dominated by Firmicutes. Comparisons with US Americans reveal microbiome differences between Africans and westerners but show western-like features in the Bantu, including an increased abundance of predictive carbohydrate and xenobiotic metabolic pathways. In contrast, the hunter-gatherer gut shows increased abundance of predicted virulence, amino acid, and Vitamin Metabolism functions, as well as dominance of lipid and amino-acid-derived metabolites, as determined through metabolomics. Our results demonstrate gradients of traditional subsistence patterns in two neighboring African groups and highlight the adaptability of the microbiome in response to host ecology.
