The Experts below are selected from a list of 168 Experts worldwide ranked by ideXlab platform
Carole Kerdelhué - One of the best experts on this subject based on the ideXlab platform.
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Microsatellite and mitochondrial data provide evidence for a single major introduction for the neartic leafhopper Scaphoideus titanus in Europe
PLoS ONE, 2012Co-Authors: Daciana Papura, C. Burban, Maarten Van Helden, Xavier Giresse, B. Nusillard, Thomas Guillemaud, Carole KerdelhuéAbstract:Scaphoideus titanus, a leafhopper native to North America and invasive in Europe, is the vector of the Flavescence dorée phytoplasma, the causal agent of the most important form of grapevine yellows in European vineyards. We studied 10 polymorphic microsatellite loci and a 623 bp fragment of the mitochondrial cytochrome oxidase II gene in native S. titanus from north-eastern America and introduced European populations, to elucidate the colonization scenario. Consistent with their recent history, invasive European populations were less genetically diverse than American populations for both types of markers, suggesting a recent bottleneck. Significant isolation by distance was detected between American populations but not between European populations. None of the European mitochondrial haplotypes was found in the American vineyards, from which they are assumed to have originated. The precise source of the invasive S. titanus populations therefore remains unclear. Nevertheless, the high heterozygosity of North-East American populations (which contained 92% of the observed alleles) suggests that this region is part of the native range of S. titanus. Clustering population genetics analyses with microsatellite and mitochondrial data suggested that European populations originated from a single introduction event. Most of the introduced populations clustered with populations from Long Island, the Atlantic Coast winegrowing region in which Vitis Aestivalis occurs.
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Microsatellite and mitochondrial data provide evidence for a single major introduction for the neartic leafhopper Scaphoideus titanus in Europe
PLoS ONE, 2012Co-Authors: Daciana Papura, C. Burban, Maarten Van Helden, Xavier Giresse, B. Nusillard, Thomas Guillemaud, Carole KerdelhuéAbstract:Scaphoideus titanus, a leafhopper native to North America and invasive in Europe, is the vector of the Flavescence doree phytoplasma, the causal agent of the most important form of grapevine yellows in European vineyards. We studied 10 polymorphic microsatellite loci and a 623 bp fragment of the mitochondrial cytochrome oxidase II gene in native S. titanus from north-eastern America and introduced European populations, to elucidate the colonization scenario. Consistent with their recent history, invasive European populations were less genetically diverse than American populations for both types of markers, suggesting a recent bottleneck. Significant isolation by distance was detected between American populations but not between European populations. None of the European mitochondrial haplotypes was found in the American vineyards, from which they are assumed to have originated. The precise source of the invasive S. titanus populations therefore remains unclear. Nevertheless, the high heterozygosity of North-East American populations (which contained 92% of the observed alleles) suggests that this region is part of the native range of S. titanus. Clustering population genetics analyses with microsatellite and mitochondrial data suggested that European populations originated from a single introduction event. Most of the introduced populations clustered with populations from Long Island, the Atlantic Coast winegrowing region in which Vitis Aestivalis occurs.
Surya Sapkota - One of the best experts on this subject based on the ideXlab platform.
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construction of a high density linkage map and qtl detection of downy mildew resistance in Vitis Aestivalis derived norton
Theoretical and Applied Genetics, 2019Co-Authors: Li-ling Chen, Surya Sapkota, Shanshan Yang, Katie E Hyma, Lance Cadledavidson, Chin-feng HwangAbstract:A major QTL for downy mildew resistance was detected on chromosome 18 (Rpv27) in Vitis Aestivalis-derived ‘Norton’ based on a high-resolution linkage map with SNP and SSR markers as well as 2 years of field and laboratory phenotyping data. Grapevine downy mildew caused by the oomycete Plasmopara viticola is one of the most widespread and destructive diseases, particularly in humid viticultural areas where it damages green tissues and defoliates vines. Traditional Vitis vinifera wine grape cultivars are susceptible to downy mildew whereas several North American and a few Asian cultivars possess various levels of resistance to this disease. To identify genetic determinants of downy mildew resistance in V. Aestivalis-derived ‘Norton,’ a mapping population with 182 genotypes was developed from a cross between ‘Norton’ and V. vinifera ‘Cabernet Sauvignon’ from which a consensus map was constructed via 411 simple sequence repeat (SSR) markers. Using genotyping-by-sequencing, 3825 single nucleotide polymorphism (SNP) markers were also generated. Of these, 1665 SNP and 407 SSR markers were clustered into 19 linkage groups in 159 genotypes, spanning a genetic distance of 2203.5 cM. Disease progression in response to P. viticola was studied in this population for 2 years under both laboratory and field conditions, and strong correlations were observed among data sets (Spearman correlation coefficient = 0.57–0.79). A quantitative trait loci (QTL) analysis indicated a resistance locus on chromosome 18, here named Rpv27, explaining 33.8% of the total phenotypic variation. Flanking markers closely linked with the trait can be further used for marker-assisted selection in the development of new cultivars with resistance to downy mildew.
Daciana Papura - One of the best experts on this subject based on the ideXlab platform.
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Microsatellite and mitochondrial data provide evidence for a single major introduction for the neartic leafhopper Scaphoideus titanus in Europe
PLoS ONE, 2012Co-Authors: Daciana Papura, C. Burban, Maarten Van Helden, Xavier Giresse, B. Nusillard, Thomas Guillemaud, Carole KerdelhuéAbstract:Scaphoideus titanus, a leafhopper native to North America and invasive in Europe, is the vector of the Flavescence dorée phytoplasma, the causal agent of the most important form of grapevine yellows in European vineyards. We studied 10 polymorphic microsatellite loci and a 623 bp fragment of the mitochondrial cytochrome oxidase II gene in native S. titanus from north-eastern America and introduced European populations, to elucidate the colonization scenario. Consistent with their recent history, invasive European populations were less genetically diverse than American populations for both types of markers, suggesting a recent bottleneck. Significant isolation by distance was detected between American populations but not between European populations. None of the European mitochondrial haplotypes was found in the American vineyards, from which they are assumed to have originated. The precise source of the invasive S. titanus populations therefore remains unclear. Nevertheless, the high heterozygosity of North-East American populations (which contained 92% of the observed alleles) suggests that this region is part of the native range of S. titanus. Clustering population genetics analyses with microsatellite and mitochondrial data suggested that European populations originated from a single introduction event. Most of the introduced populations clustered with populations from Long Island, the Atlantic Coast winegrowing region in which Vitis Aestivalis occurs.
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Microsatellite and mitochondrial data provide evidence for a single major introduction for the neartic leafhopper Scaphoideus titanus in Europe
PLoS ONE, 2012Co-Authors: Daciana Papura, C. Burban, Maarten Van Helden, Xavier Giresse, B. Nusillard, Thomas Guillemaud, Carole KerdelhuéAbstract:Scaphoideus titanus, a leafhopper native to North America and invasive in Europe, is the vector of the Flavescence doree phytoplasma, the causal agent of the most important form of grapevine yellows in European vineyards. We studied 10 polymorphic microsatellite loci and a 623 bp fragment of the mitochondrial cytochrome oxidase II gene in native S. titanus from north-eastern America and introduced European populations, to elucidate the colonization scenario. Consistent with their recent history, invasive European populations were less genetically diverse than American populations for both types of markers, suggesting a recent bottleneck. Significant isolation by distance was detected between American populations but not between European populations. None of the European mitochondrial haplotypes was found in the American vineyards, from which they are assumed to have originated. The precise source of the invasive S. titanus populations therefore remains unclear. Nevertheless, the high heterozygosity of North-East American populations (which contained 92% of the observed alleles) suggests that this region is part of the native range of S. titanus. Clustering population genetics analyses with microsatellite and mitochondrial data suggested that European populations originated from a single introduction event. Most of the introduced populations clustered with populations from Long Island, the Atlantic Coast winegrowing region in which Vitis Aestivalis occurs.
Chin-feng Hwang - One of the best experts on this subject based on the ideXlab platform.
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construction of a high density linkage map and qtl detection of downy mildew resistance in Vitis Aestivalis derived norton
Theoretical and Applied Genetics, 2019Co-Authors: Li-ling Chen, Surya Sapkota, Shanshan Yang, Katie E Hyma, Lance Cadledavidson, Chin-feng HwangAbstract:A major QTL for downy mildew resistance was detected on chromosome 18 (Rpv27) in Vitis Aestivalis-derived ‘Norton’ based on a high-resolution linkage map with SNP and SSR markers as well as 2 years of field and laboratory phenotyping data. Grapevine downy mildew caused by the oomycete Plasmopara viticola is one of the most widespread and destructive diseases, particularly in humid viticultural areas where it damages green tissues and defoliates vines. Traditional Vitis vinifera wine grape cultivars are susceptible to downy mildew whereas several North American and a few Asian cultivars possess various levels of resistance to this disease. To identify genetic determinants of downy mildew resistance in V. Aestivalis-derived ‘Norton,’ a mapping population with 182 genotypes was developed from a cross between ‘Norton’ and V. vinifera ‘Cabernet Sauvignon’ from which a consensus map was constructed via 411 simple sequence repeat (SSR) markers. Using genotyping-by-sequencing, 3825 single nucleotide polymorphism (SNP) markers were also generated. Of these, 1665 SNP and 407 SSR markers were clustered into 19 linkage groups in 159 genotypes, spanning a genetic distance of 2203.5 cM. Disease progression in response to P. viticola was studied in this population for 2 years under both laboratory and field conditions, and strong correlations were observed among data sets (Spearman correlation coefficient = 0.57–0.79). A quantitative trait loci (QTL) analysis indicated a resistance locus on chromosome 18, here named Rpv27, explaining 33.8% of the total phenotypic variation. Flanking markers closely linked with the trait can be further used for marker-assisted selection in the development of new cultivars with resistance to downy mildew.
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Interspecific hybrid identification of Vitis Aestivalis-derived ‘Norton’-based populations using microsatellite markers
Scientia Horticulturae, 2014Co-Authors: Pragya Adhikari, Li-ling Chen, Xu Chen, Surya D. Sapkota, Chin-feng HwangAbstract:Abstract The Vitis Aestivalis -derived ‘Norton’ is one of the very few commercial red grape varieties that can be grown in regions with high disease pressure and cold temperatures in winter and spring where V. vinifera is difficult to grow. This study began with the objectives of generating interspecific hybrids of Norton and V. vinifera (Cabernet Sauvignon, Pinot Noir, Syrah, and Merlot) in order to combine the disease resistance and cold hardiness characters of V. Aestivalis with the excellent wine quality of V. vinifera . Norton grapes were also crossed with the Vitis interspecific hybrid ‘Vignoles’ for the same reason. The true hybrids obtained from all of the crosses were identified using microsatellite markers. A high percentage of hybrids (70–92%) were obtained in all crosses except Norton × Merlot (17%). Results demonstrate the reliability of SSR markers for Norton-based interspecific hybrid identification.
C. Burban - One of the best experts on this subject based on the ideXlab platform.
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Microsatellite and mitochondrial data provide evidence for a single major introduction for the neartic leafhopper Scaphoideus titanus in Europe
PLoS ONE, 2012Co-Authors: Daciana Papura, C. Burban, Maarten Van Helden, Xavier Giresse, B. Nusillard, Thomas Guillemaud, Carole KerdelhuéAbstract:Scaphoideus titanus, a leafhopper native to North America and invasive in Europe, is the vector of the Flavescence dorée phytoplasma, the causal agent of the most important form of grapevine yellows in European vineyards. We studied 10 polymorphic microsatellite loci and a 623 bp fragment of the mitochondrial cytochrome oxidase II gene in native S. titanus from north-eastern America and introduced European populations, to elucidate the colonization scenario. Consistent with their recent history, invasive European populations were less genetically diverse than American populations for both types of markers, suggesting a recent bottleneck. Significant isolation by distance was detected between American populations but not between European populations. None of the European mitochondrial haplotypes was found in the American vineyards, from which they are assumed to have originated. The precise source of the invasive S. titanus populations therefore remains unclear. Nevertheless, the high heterozygosity of North-East American populations (which contained 92% of the observed alleles) suggests that this region is part of the native range of S. titanus. Clustering population genetics analyses with microsatellite and mitochondrial data suggested that European populations originated from a single introduction event. Most of the introduced populations clustered with populations from Long Island, the Atlantic Coast winegrowing region in which Vitis Aestivalis occurs.
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Microsatellite and mitochondrial data provide evidence for a single major introduction for the neartic leafhopper Scaphoideus titanus in Europe
PLoS ONE, 2012Co-Authors: Daciana Papura, C. Burban, Maarten Van Helden, Xavier Giresse, B. Nusillard, Thomas Guillemaud, Carole KerdelhuéAbstract:Scaphoideus titanus, a leafhopper native to North America and invasive in Europe, is the vector of the Flavescence doree phytoplasma, the causal agent of the most important form of grapevine yellows in European vineyards. We studied 10 polymorphic microsatellite loci and a 623 bp fragment of the mitochondrial cytochrome oxidase II gene in native S. titanus from north-eastern America and introduced European populations, to elucidate the colonization scenario. Consistent with their recent history, invasive European populations were less genetically diverse than American populations for both types of markers, suggesting a recent bottleneck. Significant isolation by distance was detected between American populations but not between European populations. None of the European mitochondrial haplotypes was found in the American vineyards, from which they are assumed to have originated. The precise source of the invasive S. titanus populations therefore remains unclear. Nevertheless, the high heterozygosity of North-East American populations (which contained 92% of the observed alleles) suggests that this region is part of the native range of S. titanus. Clustering population genetics analyses with microsatellite and mitochondrial data suggested that European populations originated from a single introduction event. Most of the introduced populations clustered with populations from Long Island, the Atlantic Coast winegrowing region in which Vitis Aestivalis occurs.
