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Caroline Dani - One of the best experts on this subject based on the ideXlab platform.
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the impact of red grape juice Vitis Labrusca consumption associated with physical training on oxidative stress inflammatory and epigenetic modulation in healthy elderly women
Physiology & Behavior, 2021Co-Authors: Caroline Dani, Karen Moura Dias, Lucieli Trevizol, Luisa Bassoa, Iasmin Fraga, Isabel Cristina Teixeira Proenca, Daniela Pochmann, Viviane Rostirola ElsnerAbstract:Abstract The imbalance of epigenetic, oxidative stress and inflammatory markers is associated with the aging physiopathology. Then, the influence of bioactive nutritional compounds and physical training on these biomarkers has been studied, although the combination of both strategies has been not investigated. Therefore, our aim was to verify the effect of the association of physical training with red grape juice (Vitis Labrusca) consumption on global histone acetylation H3 and H4 levels, oxidative stress markers and interleukin 6 (IL-6) levels in peripheral blood of healthy elderly women. This double-blind randomized clinical study consisted of 29 volunteers, aged 59 years and over, divided into three groups: grape juice group (GJG, n = 9); placebo and exercise group (PLEG, n = 10) and grape juice and exercise group (GJEG, n = 10). During 1 month, GJG consumed 400 ml of grape juice per day (integral and conventional), while the PLEG and GJEG groups, besides consuming juice or placebo were submitted to a concurrent physical training protocol (two times per week, 60 min / session). The volunteers were submitted to blood collections before and after the intervention for the biomarkers analysis, e.g. IL-6, histone acetylation H3 and H4, lipid oxidative damage (TBARS), proteins (Carbonyl), non-enzymatic antioxidant defense (Sulfhydryl groups) and activity of antioxidant enzymes (superoxide dismutase and catalase). There were no statistically significant differences in the global levels of histone acetylation H3 and H4 post intervention compared to the basal period as well and between groups were found. However, PLEG and GJEG showed a remarkable reduction on IL-6 levels after intervention. We also observed an increase in Carbonyl levels, SOD activities and Sulfhydryl levels comparing before and after intervention. Considering the interaction of time and groups, a significant increase in Sulfhydryl levels only in GJG was found. The physical training protocol associated or not with grape juice consumption showed anti-inflammatory effects and an influence in the antioxidant defenses (non enzymatic and enzymatic) in elderly women. However in grape juice group, without exercise, we observed an increase in non enzymatic antioxidant defense, what could be attributed to the polyphenols content. These responses seem not to be involved with histone acetylation status.
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the impact of red grape juice Vitis Labrusca consumption associated with physical training on oxidative stress inflammatory and epigenetic modulation in healthy elderly women
Physiology & Behavior, 2020Co-Authors: Caroline Dani, Karen Moura Dias, Lucieli Trevizol, Luisa Bassoa, Iasmin Fraga, Isabel Cristina Teixeira Proenca, Daniela Pochmann, Viviane Rostirola ElsnerAbstract:Abstract The imbalance of epigenetic, oxidative stress and inflammatory markers is associated with the aging physiopathology. Then, the influence of bioactive nutritional compounds and physical training on these biomarkers has been studied, although the combination of both strategies has been not investigated. Therefore, our aim was to verify the effect of the association of physical training with red grape juice (Vitis Labrusca) consumption on global histone acetylation H3 and H4 levels, oxidative stress markers and interleukin 6 (IL-6) levels in peripheral blood of healthy elderly women. This double-blind randomized clinical study consisted of 29 volunteers, aged 59 years and over, divided into three groups: grape juice group (GJG, n = 9); placebo and exercise group (PLEG, n = 10) and grape juice and exercise group (GJEG, n = 10). During 1 month, GJG consumed 400 ml of grape juice per day (integral and conventional), while the PLEG and GJEG groups, besides consuming juice or placebo were submitted to a concurrent physical training protocol (two times per week, 60 minutes / session). The volunteers were submitted to blood collections before and after the intervention for the biomarkers analysis, e.g. IL-6, histone acetylation H3 and H4, lipid oxidative damage (TBARS), proteins (Carbonyl), non-enzymatic antioxidant defense (Sulfhydryl groups) and activity of antioxidant enzymes (superoxide dismutase and catalase). There were no statistically significant differences in the global levels of histone acetylation H3 and H4 post intervention compared to the basal period as well and between groups were found. However, PLEG and GJEG showed a remarkable reduction on IL-6 levels after intervention. We also observed an increase in Carbonyl levels, SOD activities and Sulfhydryl levels comparing before and after intervention. Considering the interaction of time and groups, a significant increase in Sulfhydryl levels only in GJG was found. The physical training protocol associated or not with grape juice consumption showed anti-inflammatory effects and an influence in the antioxidant defenses (non enzymatic and enzymatic) in elderly women. However in grape juice group, without exercise, we observed an increase in non enzymatic antioxidant defense, what could be attributed to the polyphenols content. These responses seem not to be involved with histone acetylation status.
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Evaluation of antioxidant activity of grapevine leaves extracts (Vitis Labrusca) in liver of Wistar rats.
Anais da Academia Brasileira de Ciencias, 2016Co-Authors: Thays Krischke Schaffer, Cláudia Funchal, Fabiana Agostini, Niara Da Silva Medeiros, Mariane Farias Wohlenberg, Jéssica Berghahn Martins, Caroline DaniAbstract:The aim of this study was to evaluate the hepatoprotection of organic and conventional grapevine leaves extract (Vitis Labrusca). The total polyphenol content and the isolate polyphenols by HPLC were evaluate. The animals received intraperitoneal injections of saline or extracts (conventional or organic - 30 mg/kg) for 14 days. On day 15, the rats received carbon tetrachloride (CCl4) or mineral oil (i.p.). After 4h, the animals were euthanized. The analysis of the liver enzymes activity (AST, ALT, GGT) was performed using serum, obtained by blood and the levels of lipid peroxidation (TBARS), protein oxidation (carbonyl), and the activity of antioxidant enzymes superoxide dismutase and catalase were analyzed in the liver. The results showed that the organic extract is richer in polyphenol and resveratrol than the conventional one. Both extracts prevent lipid peroxidation and protein oxidation generated by CCl4. Moreover, the extracts demonstrated ability to modulate the activity of SOD and CAT, as well as to establish a balance in the ratio of SOD/CAT. We also found that the CCl4 increased the levels of AST and GGT, and that both extracts prevent this. These results indicate that grapevine leaves extracts, both, organic and conventional, can prevent liver disorders.
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Method validation for determination of metals in Vitis Labrusca L. grapevine leaf extracts by inductively coupled plasma mass spectrometry (ICP-MS).
Anais da Academia Brasileira de Ciencias, 2016Co-Authors: Liane Valadão Vieira Bokowski, Roberto B. Sobrinho, Cristián J.v. Armijo, Caroline Dani, João Antonio Pêgas Henriques, Cláudia FunchalAbstract:Vitis Labrusca L. is the main species used for wine and juice production in Brazil. The grapevine leaves can be used both as functional foods and as cheapest sources for the extraction of phenolic compounds. Besides the antioxidant activity, grapevine leaves exhibited significant anti-inflammatory activity. Therefore, the aim of this study was to develop and validate an analytical methodology to determine the metals selenium (96Se), chromium (53Cr), nickel (62Ni), cadmium (111Cd) and lead (206Pb) in 30 samples of grapevine leaf extracts (Vitis Labrusca, Bordo cultivar) using inductively coupled plasma mass spectrometry (ICP-MS). To obtain the grapevine leaf extracts the samples were milled, weighed and digested in microwave oven with nitric acid. The method showed linearity, precision, accuracy and limits of quantification and detection acceptable for INMETRO protocol validation of analytical methods. Therefore, the method using ICP-MS was developed and validated to determine metals concentrations in grapevine leaves of Vitis Labrusca L. and the proposed method could be applied in routine analytical laboratory.
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Vitis Labrusca leaf extract prevents pentylenetetrazol-induced oxidative damage but not seizures in rats.
Cellular and molecular biology (Noisy-le-Grand France), 2015Co-Authors: Adriana Simon Coitinho, Mirian Salvador, B M Da Costa, M T Dos Santos, Bruna Fank, C L Hackmann, Caroline DaniAbstract:Epilepsy is a disorder of the central nervous system characterized by recurrent seizures. It is a very common disease in which approximately 30% of patients do not respond favorably to treatment with anticonvulsants. Oxidative stress is associated with neuronal damage arising from epileptic seizures. The present study investigated the possible anticonvulsant and antioxidant effects of a leaf extract of Vitis Labrusca in an animal model of seizures induced by pentylenetetrazole (PTZ). The animals received injections of V. Labrusca extract (10, 30 and 100 mg/kg) or vehicle and, 30 minutes later, they received an injection of PTZ, and were then observed for 30 minutes. The latency time and tonic-clonic seizure time were registered. Oxidative damage in lipids and proteins was quantified in the cerebellum, cerebral cortex and hippocampus. It was observed that the leaf extract were capable of reducing lipid peroxidation and protein oxidation caused by PTZ at all doses tested.
Mirian Salvador - One of the best experts on this subject based on the ideXlab platform.
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Green synthesis of silver nanoparticles using an extract of Ives cultivar (Vitis Labrusca) pomace: Characterization and application in wastewater disinfection
Journal of Environmental Chemical Engineering, 2019Co-Authors: Camila S. Raota, Mirian Salvador, Sergio Echeverrigaray, Aline Fagundes Cerbaro, Ana Paula Longaray Delamare, Janaina S. Crespo, Thiago Barcellos Da Silva, Marcelo GiovanelaAbstract:Abstract Traditional methods for synthesizing metallic nanoparticles include the use of toxic chemicals, which can have negative impacts on the environment and human health. A safer alternative to produce these nanomaterials is based on the concept of Green Chemistry, which uses less harmful chemicals from renewable sources. In this work, we describe a specific approach for the green synthesis of silver nanoparticles (AgNPs) using the extract of Ives cultivar (Vitis Labrusca) pomace, their characterization, and their immobilization in a polymer matrix of chitosan aiming the disinfection of a raw wastewater sample. The influence of some experimental parameters on synthesis such as pH, stabilization time as well as the temperature has been investigated. The grape pomace extract was initially characterized regarding its content of phenolic compounds and then used as stabilizing-reducing agent. The obtained AgNPs were analyzed by ultraviolet-visible spectroscopy, transmission electron microscopy, zeta potential, scanning electron microscopy coupled with energy dispersive spectroscopy, and raman spectroscopy. In general, the AgNPs were spherical in shape and very stable. Aiming to explain the reaction, we also proposed a mechanism of formation of AgNPs based on gallic acid molecule. Concerning the antimicrobial properties, the results demonstrated the synergistic activity of chitosan-AgNPs pellets against Gram-positive and Gram-negative bacteria. Additionally, these materials led to a reduction of 47% of the bacterial count of Escherichia coli on the disinfection of the wastewater. Finally, it was concluded that the extract of Ives cultivar pomace is capable of producing stable AgNPs that can be used as auxiliaries in wastewater disinfection.
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Vitis Labrusca leaf extract prevents pentylenetetrazol-induced oxidative damage but not seizures in rats.
Cellular and molecular biology (Noisy-le-Grand France), 2015Co-Authors: Adriana Simon Coitinho, Mirian Salvador, B M Da Costa, M T Dos Santos, Bruna Fank, C L Hackmann, Caroline DaniAbstract:Epilepsy is a disorder of the central nervous system characterized by recurrent seizures. It is a very common disease in which approximately 30% of patients do not respond favorably to treatment with anticonvulsants. Oxidative stress is associated with neuronal damage arising from epileptic seizures. The present study investigated the possible anticonvulsant and antioxidant effects of a leaf extract of Vitis Labrusca in an animal model of seizures induced by pentylenetetrazole (PTZ). The animals received injections of V. Labrusca extract (10, 30 and 100 mg/kg) or vehicle and, 30 minutes later, they received an injection of PTZ, and were then observed for 30 minutes. The latency time and tonic-clonic seizure time were registered. Oxidative damage in lipids and proteins was quantified in the cerebellum, cerebral cortex and hippocampus. It was observed that the leaf extract were capable of reducing lipid peroxidation and protein oxidation caused by PTZ at all doses tested.
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Vitis Labrusca extract effects on cellular dynamics and redox modulations in a SH-SY5Y neuronal cell model: A similar role to lithium
Neurochemistry international, 2014Co-Authors: Gustavo Scola, Mirian Salvador, Victoria Louise Marina Laliberte, Helena Kyunghee Kim, Arsene Pinguelo, L. Trevor Young, Ana Cristina AndreazzaAbstract:Oxidative stress and calcium imbalance are consistently reported in bipolar disorder (BD). Polymorphism of voltage-dependent calcium channel, L type, alpha 1C subunit (CACNA1c), which is responsible for the regulation of calcium influx, was also shown to have a strong association with BD. These alterations can lead to a number of different consequences in the cell including production of reactive species causing oxidative damage to proteins, lipids and DNA. Lithium is the most frequent medication used for the treatment of BD. Despite lithium's effects, long-term use can result in many negative side effects. Therefore, there is an urgent need for the development of drugs that may have similar biological effects as lithium without the negative consequences. Moreover, polyphenols are secondary metabolites of plants that present multi-faceted molecular abilities, such as regulation of cellular responses. Vitis Labrusca extract (VLE), a complex mixture of polyphenols obtained from seeds of winery wastes of V. Labrusca, was previously characterized by our group. This extract presented powerful antioxidant and neuroprotective properties. Therefore, the ability of VLE to ameliorate the consequences of hydrogen peroxide (H2O2)-induced redox alterations to cell viability, intracellular calcium levels and the relative levels of the calcium channel CACNA1c in comparison to lithium's effects were evaluated using a neuroblastoma cell model. H2O2 treatment increased cell mortality through apoptotic and necrotic pathways leading to an increase in intracellular calcium levels and alterations to relative CACNA1c levels. VLE and lithium were found to similarly ameliorate cell mortality through regulation of the apoptotic/necrotic pathways, decreasing intracellular calcium levels and preventing alterations to the relative levels of CACNA1c. The findings of this study suggest that VLE exhibits protective properties against oxidative stress-induced alterations similar to that of lithium. These findings suggest that VLE may be an attractive potential candidate as a novel therapeutic agent for BD.
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Suppression of Oncoprotein Her-2 and DNA Damage after Treatment with Flavan-3- ol Vitis Labrusca Extract
Anti-cancer agents in medicinal chemistry, 2013Co-Authors: Gustavo Scola, Claudia Cilene Fernandes Correia Laurino, Eveline Menin, Mirian SalvadorAbstract:Hepatocellular carcinoma and breast cancer are the most prevalent cancers in the world with high morbidity and mortality. Although there are effective drugs for treating advanced stages of liver and breast cancers, the prognosis for patients with liver cancer remains poor, and patients with breast cancer show considerable mortality. Therefore, it is crucial to explore new therapeutic agents for the inhibition of carcinogenesis. This study examined the anti-carcinogenic effect of Vitis Labrusca seed extract (VLE), which is a component of winery waste, on liver (HepG2) and breast cancers (MCF-7) cells. The results found in this study demonstrated VLEinduced DNA damage in liver and breast cancer cells. VLE treatment in both cell lines was accompanied by high NO production and upregulation of p53. A significant decrease in total PARP expression was also found in HepG2 cells. In the MCF-7 cell line, VLE treatment increased the expression of Bax and AIF, and decreased total PARP expression. Surprisingly, VLE suppressed Her-2 expression in HepG2 cells and caused a subtle, but significant downregulation of Her-2 in MCF-7 cells. The possible anti-carcinogenic effect of VLE reported in this study suggests the potential of this extract to be used for the development of novel therapeutic agents for the treatment of different kinds of cancers.
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Hepatoprotective, cardioprotective, and renal-protective effects of organic and conventional grapevine leaf extracts (Vitis Labrusca var. Bordo) on Wistar rat tissues
Anais da Academia Brasileira de Ciencias, 2011Co-Authors: Lívia S. Oliboni, Caroline Dani, João Antonio Pêgas Henriques, Cláudia Funchal, Mirian SalvadorAbstract:The purpose of this study was to evaluate the beneficial effects of organic and conventional grapevine ( Vitis Labrusca L.) leaf extracts in reducing hydrogen peroxide-induced stress in the liver, heart and kidney of Wistar rats by measuring lipids and proteins damages (carbonyl assay), as well as the activity of the antioxidant enzymes superoxide dismutase and catalase. The preincubation with 5 mg/mL of organic and conventional grapevine (Vitis Labrusca L.) leaf extracts prevented both lipids and proteins oxidative damages in all tissues analyzed. The organic leaf extract was able to restore superoxide dismutase (kidney and liver) and catalase (heart) activities, which were modified by the treatment with H 2 O 2 . The conventional extract was able to restore only the catalase activity in liver and heart tissues. The beneficial effects of the V. Labrusca leaf extract shown in this study could probably be important for formulating dietary supplements, as well as for developing new ingredients with improved antioxidant properties from other plant sources.
Sergio Ruffo Roberto - One of the best experts on this subject based on the ideXlab platform.
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exogenous abscisic acid promotes anthocyanin biosynthesis and increased expression of flavonoid synthesis genes in Vitis vinifera Vitis Labrusca table grapes in a subtropical region
Frontiers in Plant Science, 2018Co-Authors: Renata Koyama, Sergio Ruffo Roberto, Reginaldo Teodoro De Souza, Wellington Fernando Silva Borges, Mauri Anderson, Andrew L Waterhouse, Dario Cantu, Matthew W Fidelibus, Barbara BlancoulateAbstract:Hybrid (Vitis vinifera ×Vitis Labrusca) table grape cultivars grown in the subtropics often fail to accumulate sufficient anthocyanins to achieve good uniform berry color. Growers of V. vinifera table grapes in temperate regions generally use ethephon and, more recently, (S)-cis-abscisic acid (S-ABA) to overcome this problem. The objective of this study was to determine if S-ABA applications at different timings and concentrations have an effect on anthocyanin regulatory and biosynthetic genes, pigment accumulation, and berry color of the Selection 21 cultivar, a new V. vinifera ×V. Labrusca hybrid seedless grape that presents lack of red color when grown in subtropical areas. Applications of S-ABA 400 mg/L resulted in a higher accumulation of total anthocyanins and of the individual anthocyaninsanthocyanins: delphinidin-3-glucoside, cyanidin-3-glucoside, peonidin-3-glucoside, and malvidin-3-glucoside in the berry skin and improved the color attributes of the berries. Treatment with two applications at 7 days after veraison (DAV) and 21 DAV of S-ABA 400 mg/L resulted in a higher accumulation of total anthocyanins in the skin of berries and increased the gene expression of CHI, F3H, DFR, and UFGT and of the VvMYBA1 and VvMYBA2 transcription factors in the seedless grape cultivar.
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application of abscisic acid s aba to cv isabel grapes Vitis vinifera Vitis Labrusca for color improvement effects on color phenolic composition and antioxidant capacity of their grape juice
Food Research International, 2015Co-Authors: Lilian Yukari Yamamoto, Sergio Ruffo Roberto, Esteban Garciaromero, Adriane Marinho De Assis, Yuri Renan Bovolenta, Suzana Lucy Nixdorf, Sergio Gomezalonso, Isidro HermosingutierrezAbstract:Abstract Hybrid grape Isabel ( Vitis vinifera × Vitis Labrusca ) is the main cultivar used to produce grape juice in Brazil. However Isabel grape is deficient in anthocyanins. Application of abscisic acid ( S -ABA), which is responsible for promoting the anthocyanin synthesis, offers a way for color improvement. This study evaluates the changes on the phenolic composition of Isabel grape juice produced from grapes treated with S -ABA, over two consecutive harvest seasons. Application of S -ABA to grapes increased the content of total anthocyanins in the corresponding juices and also the proportion of anthocyanins based on B-ring tri-substituted anthocyanidins. Regarding non-anthocyanin phenolic compounds, total flavonol content was only significantly higher in juices produced from grapes treated in the 2012 season, whereas the treatment did not significantly affect the content of hydroxycinnamic acid derivatives, flavan-3-ols, resveratrol and even the antioxidant capacity of juices. Juice from Isabel grapes treated with S -ABA provides an enhancement of total anthocyanin concentration, mainly when grapes are treated before or at the onset of veraison. As a consequence, the color intensity increased in all the juices obtained from treated grapes.
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Fenologia e soma termica (graus-dia) para a videira ‘Isabel’ (Vitis Labrusca) cultivada no Noroeste do Parana
Semina: Ciências Agrárias, 2004Co-Authors: Sergio Ruffo Roberto, Alessandro Jefferson Sato, Everton Allen Brenner, Cristiano Ezequiel Dos Santos, Werner GentaAbstract:O trabalho teve como objetivo caracterizar o comportamento fenologico da videira ‘Isabel’ (Vitis Labrusca) cultivada no norte do Estado do Parana, bem como caracterizar sua exigencia termica em graus-dia. A area experimental foi instalada em uma propriedade comercial pertencente a Vinicola Intervin®, localizada no municipio de Maringa, PR. O vinhedo foi estabelecido em agosto de 2000 e as plantas foram conduzidas no sistema latada no espacamento de 4,0 m x 1,0 m. As avaliacoes tiveram inicio a partir da poda de frutificacao realizada no fim do inverno de 2003. O delineamento experimental foi o inteiramente casualizado com 20 repeticoes, sendo cada parcela constituida por uma unica planta. A fenologia das plantas foi avaliada quanto a duracao em dias de cada um dos seguintes subperiodos: poda a gema-algodao; poda a brotacao; poda ao aparecimento da inflorescencia; poda ao florescimento; poda ao inicio da maturacao das bagas e poda a colheita. A exigencia termica da uva ‘Isabel’ foi calculada empregando-se o somatorio de graus-dia desde a poda ate a colheita, bem como para cada um dos subperiodos, considerando-se duas temperaturas-base (10oC e 12oC). Pelos resultados obtidos conclui-se que: a duracao do ciclo da poda a colheita da videira ‘Isabel’ no norte do Parana e de 127 dias, e a sua exigencia termica e de 1.238,20 graus-dias, sendo a temperatura-base de 10oC a mais adequada para o calculo desta demanda.
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Fenologia e soma térmica (graus-dia) para a videira 'Isabel' (Vitis Labrusca) cultivada no Noroeste do Paraná 1 Phenology and thermal summation (degree days) for 'Isabel' grape (Vitis Labrusca) produced in the Northwest of Parana State
2004Co-Authors: Sergio Ruffo Roberto, Alessandro Jefferson Sato, Everton Allen Brenner, Cristiano Ezequiel, Werner GentaAbstract:The objetive of this research was to characterize the phenological performance of ‘Isabel’ grape (Vitis Labrusca) produced in the northwest of Parana state, as well to characterize its thermal demand in degree-days. The experimental area was established in a commercial vineyard of Vinicola Intervin®, Maringa, PR. The vineyard was planted in August of 2000 and the trees were trained in a pergola system, in a 4.0 x 1.0 m spacing. The evaluations started from the winter pruning of 2003. The random design was used as the statistical model with 20 replications and each plot was composed by one tree. The phenology 1 Parte do projeto apoiado pela International Foundation for Science, IFS, Suecia; Fundacao Araucaria e CNPq. 2
Marilde T. Bordignon-luiz - One of the best experts on this subject based on the ideXlab platform.
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Bioactive potential of Vitis Labrusca L. grape juices from the Southern Region of Brazil: phenolic and elemental composition and effect on lipid peroxidation in healthy subjects.
Food chemistry, 2014Co-Authors: Isabela Maia Toaldo, Fernanda Alves Cruz, Tatiana De Lima Alves, Jefferson Santos De Gois, Daniel L. G. Borges, Heloisa Pamplona Cunha, Edson Luiz Da Silva, Marilde T. Bordignon-luizAbstract:Grapes are rich in polyphenols with biologically active properties. Although the bioactive potential of grape constituents are frequently reported, the effects of Brazilian Vitis Labrusca L. grape juices ingestion have not been demonstrated in humans. This study identified the phenolic and elemental composition of red and white grape juices and the effect of organic and conventional red grape juice consumption on lipid peroxidation in healthy individuals. Concentrations of anthocyanins, flavanols and phenolic acids and the in vitro antioxidant activity were significantly higher in the organic juice. The macro-elements K, Ca, Na and Mg were the most abundant minerals in all juices. The acute consumption of red grape juices promoted significant decrease of lipid peroxides in serum and TBARS levels in plasma. It is concluded that red V. Labrusca L. grape juices produced in Southern Brazil showed lipid peroxidation inhibition abilities in healthy subjects, regardless of the cultivation system.
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Bioactive compounds and antioxidant activity of Vitis vinifera and Vitis Labrusca grapes: Evaluation of different extraction methods
Microchemical Journal, 2014Co-Authors: Vívian Maria Burin, Nayla E. Ferreira-lima, Carolina P. Panceri, Marilde T. Bordignon-luizAbstract:Abstract Polyphenols have been associated with the bioactive potential of grapes due to their beneficial health effects. For this reason, the aim of this study was to determine the content of bioactive compounds and in vitro antioxidant activity of selected Vitis vinifera and Vitis Labrusca grapes widely produced in Brazil. In order to evaluate the efficiency of different methods for the extraction of the main polyphenols in grape, four methods were tested and compared. Among the extraction methods evaluated, liquid–liquid extraction was selected as the best method, which showed the best precision and repeatability. The phenolic compounds of white and red V. vinifera and V. Labrusca grapes indicated that the type and concentration of polyphenols were mainly dependent on the grape variety rather than the species. The antioxidant activity of all grape samples evaluated was strongly correlated to the trans -resveratrol content (R > 0.9). Among all samples, it was observed that V. Labrusca varieties, especially Bordo and Concord grapes widely cultivated in the south of Brazil, presented higher antioxidant potential, associated with the presence of higher content of bioactive compounds.
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Effect of grape seeds on the polyphenol bioactive content and elemental composition by ICP-MS of grape juices from Vitis Labrusca L.
LWT - Food Science and Technology, 2013Co-Authors: Isabela Maia Toaldo, Jefferson Santos De Gois, Daniel L. G. Borges, Odinei Fogolari, Gabriela Cadore Pimentel, Vinícius Caliari, Marilde T. Bordignon-luizAbstract:Abstract The effect of grape seeds on the bioactive content and elemental composition of grape juices from Vitis Labrusca L. varieties Concord, Isabel and Bordo, widely cultivated in Brazil, was examined. Grape seeds of each variety were macerated with berries at concentrations of 50, 100 and 200 g/kg of grape, followed by the extraction of varietal juices. The total phenolic content, monomeric anthocyanins and antioxidant capacity were determined in juices, and their correlations with seeds were assessed. The mineral content and metal contamination in juices was determined by ICP-MS. Grape seeds significantly increased the total phenolics and antioxidant capacity of juices, showing high correlations. In Isabel juices, seed addition at concentration of 200 g/kg increased about 8 times the polyphenols concentration. The addition of seeds was poorly correlated with anthocyanins content and positively correlated with minerals (Ca, Mg, Na, K, Mn, Zn). The trace elements Cu, Ni, Sr, As, Cr, Li, Ba and Al were detected. The inclusion of seeds in grape juice comprises a feasible approach to bioactive enrichment of this widely appreciated beverage and also an alternative to reduce vegetable waste in fruit industries.
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Colour stability of anthocyanins from Isabel grapes (Vitis Labrusca L.) in model systems
LWT - Food Science and Technology, 2007Co-Authors: Marilde T. Bordignon-luiz, Cony Gauche, Eliana Fortes Gris, Leila Denise FalcãoAbstract:Abstract The influence of temperature, pH, the presence or absence of light, the presence or absence of oxygen and the presence or absence of tannic acid on the colour stability of anthocyanins from crude extract of Isabel grapes ( Vitis Labrusca L.) in food and model systems was studied. The spectrophotometric results (Δ λ , Δ A ) revealed interaction between the anthocyanins of the crude extract and tannic acid, suggesting copigmentation. In a model system, temperature, pH, the presence or absence of light, the presence or absence of oxygen and the presence or absence of tannic acid, significantly affected the half-life time of anthocyanins. The long half-time of the anthocyanins from Isabel grapes in a yoghurt model system indicates that these pigments are relatively stable in this food. In a rehydrated beverage model system at 4±1 °C, the presence of tannic acid (1:1, w/v) increased the half-life time by 187 h compared to the control samples.
Cláudia Funchal - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of antioxidant activity of grapevine leaves extracts (Vitis Labrusca) in liver of Wistar rats.
Anais da Academia Brasileira de Ciencias, 2016Co-Authors: Thays Krischke Schaffer, Cláudia Funchal, Fabiana Agostini, Niara Da Silva Medeiros, Mariane Farias Wohlenberg, Jéssica Berghahn Martins, Caroline DaniAbstract:The aim of this study was to evaluate the hepatoprotection of organic and conventional grapevine leaves extract (Vitis Labrusca). The total polyphenol content and the isolate polyphenols by HPLC were evaluate. The animals received intraperitoneal injections of saline or extracts (conventional or organic - 30 mg/kg) for 14 days. On day 15, the rats received carbon tetrachloride (CCl4) or mineral oil (i.p.). After 4h, the animals were euthanized. The analysis of the liver enzymes activity (AST, ALT, GGT) was performed using serum, obtained by blood and the levels of lipid peroxidation (TBARS), protein oxidation (carbonyl), and the activity of antioxidant enzymes superoxide dismutase and catalase were analyzed in the liver. The results showed that the organic extract is richer in polyphenol and resveratrol than the conventional one. Both extracts prevent lipid peroxidation and protein oxidation generated by CCl4. Moreover, the extracts demonstrated ability to modulate the activity of SOD and CAT, as well as to establish a balance in the ratio of SOD/CAT. We also found that the CCl4 increased the levels of AST and GGT, and that both extracts prevent this. These results indicate that grapevine leaves extracts, both, organic and conventional, can prevent liver disorders.
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Method validation for determination of metals in Vitis Labrusca L. grapevine leaf extracts by inductively coupled plasma mass spectrometry (ICP-MS).
Anais da Academia Brasileira de Ciencias, 2016Co-Authors: Liane Valadão Vieira Bokowski, Roberto B. Sobrinho, Cristián J.v. Armijo, Caroline Dani, João Antonio Pêgas Henriques, Cláudia FunchalAbstract:Vitis Labrusca L. is the main species used for wine and juice production in Brazil. The grapevine leaves can be used both as functional foods and as cheapest sources for the extraction of phenolic compounds. Besides the antioxidant activity, grapevine leaves exhibited significant anti-inflammatory activity. Therefore, the aim of this study was to develop and validate an analytical methodology to determine the metals selenium (96Se), chromium (53Cr), nickel (62Ni), cadmium (111Cd) and lead (206Pb) in 30 samples of grapevine leaf extracts (Vitis Labrusca, Bordo cultivar) using inductively coupled plasma mass spectrometry (ICP-MS). To obtain the grapevine leaf extracts the samples were milled, weighed and digested in microwave oven with nitric acid. The method showed linearity, precision, accuracy and limits of quantification and detection acceptable for INMETRO protocol validation of analytical methods. Therefore, the method using ICP-MS was developed and validated to determine metals concentrations in grapevine leaves of Vitis Labrusca L. and the proposed method could be applied in routine analytical laboratory.
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Antioxidant and hepatoprotective effects of an organic grapevine leaf (Vitis Labrusca L.) extract in diabetic rats
RSC Adv., 2014Co-Authors: Denise Dos Santos Lacerda, Caroline Dani, Cláudia Funchal, Carolina Ferreira Santos, Alice S. Oliveira, Rafaela Zimmermann, Ricardo Schneider, Fabiana Agostini, Rosane GomezAbstract:Our objective was to investigate the antioxidant effect of an aqueous extract of organic grapevine leaves (Vitis Labrusca L.) on the livers of diabetic rats and to evaluate the resulting changes in metabolic and biochemical parameters. Diabetic rats received daily intragastric doses of 50, 100 or 200 mg kg−1 of the grapevine extract for 30 days. Grapevine leaf extract showed a dose-dependent antioxidant effect on the livers of diabetic rats, evidenced by decreases in TBARS and in carbonyl levels and increases in sulfhydryl levels. Moreover, the extract (200 mg kg−1) prevented weight loss and reduced LDL cholesterol (50 mg kg−1), urea (50 mg kg−1), and AST (50 and 100 mg kg−1) levels in diabetic rats at the indicated doses. Thus, we suggest that chronic treatment with an extract of grapevine leaves may represent an adjuvant therapy for the treatment and/or prevention of diabetic complications because of its antioxidant, hepatoprotective and possible hypolipidemic effects shown here.
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Effect of Chronic Treatment with Conventional and Organic Purple Grape Juices (Vitis Labrusca) on Rats Fed with High-Fat Diet
Cellular and Molecular Neurobiology, 2013Co-Authors: Marcia Gilceane Cardozo, Caroline Dani, João Antonio Pêgas Henriques, Niara Medeiros, Denise Santos Lacerda, Daniela Campos Almeida, Cláudia FunchalAbstract:Serra Gaucha is described as the most important wine region of Brazil. Regarding cultivars widespread in the Serra Gaucha, about 90 % of the area is occupied by vines of Vitis Labrusca that is the most important specie used in grape juice production. The objective of this study was to investigate the antioxidant and neuroprotective effect of chronic intake of purple grape juice (organic and conventional) from Bordo variety ( V. Labrusca ) on oxidative stress in different brain regions of rats supplemented with high-fat diet (HFD) for 3 months. A total of 40 male rats were randomly divided into 4 groups. Group 1 received a standard diet and water, group 2 HFD and water, group 3 HFD and conventional grape juice (CGJ), and group 4 HFD and organic grape juice (OGJ). All groups had free access to food and drink and after 3 months of treatment the rats were euthanized by decapitation and the cerebral cortex, hippocampus and cerebellum isolated and homogenized on ice for oxidative stress analysis. We observed that the consumption of calories in HFD and control groups, were higher than the groups supplemented with HFD and grape juices and that HFD diet group gain more weight than the other animals. Our results also demonstrated that HDF enhanced lipid peroxidation (TBARS) and protein damage (carbonyl) in cerebral cortex and hippocampus, reduced the non-enzymatic antioxidants defenses (sulfhydryl) in cerebral cortex and cerebellum, reduced catalase and superoxide dismutase activities in all brain tissues and enhanced nitric oxide production in all cerebral tissues. CGJ and OGJ were able to ameliorate these oxidative alterations, being OGJ more effective in this protection. Therefore, grape juices could be useful in the treatment of some neurodegenerative diseases associated with oxidative damage.
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Hepatoprotective, cardioprotective, and renal-protective effects of organic and conventional grapevine leaf extracts (Vitis Labrusca var. Bordo) on Wistar rat tissues
Anais da Academia Brasileira de Ciencias, 2011Co-Authors: Lívia S. Oliboni, Caroline Dani, João Antonio Pêgas Henriques, Cláudia Funchal, Mirian SalvadorAbstract:The purpose of this study was to evaluate the beneficial effects of organic and conventional grapevine ( Vitis Labrusca L.) leaf extracts in reducing hydrogen peroxide-induced stress in the liver, heart and kidney of Wistar rats by measuring lipids and proteins damages (carbonyl assay), as well as the activity of the antioxidant enzymes superoxide dismutase and catalase. The preincubation with 5 mg/mL of organic and conventional grapevine (Vitis Labrusca L.) leaf extracts prevented both lipids and proteins oxidative damages in all tissues analyzed. The organic leaf extract was able to restore superoxide dismutase (kidney and liver) and catalase (heart) activities, which were modified by the treatment with H 2 O 2 . The conventional extract was able to restore only the catalase activity in liver and heart tissues. The beneficial effects of the V. Labrusca leaf extract shown in this study could probably be important for formulating dietary supplements, as well as for developing new ingredients with improved antioxidant properties from other plant sources.
