The Experts below are selected from a list of 234 Experts worldwide ranked by ideXlab platform
Nakazato G - One of the best experts on this subject based on the ideXlab platform.
-
Serotyping of Streptococcus suis strains isolated from pigs in the states of Sao Paulo, Minas Gerais and Parana, Brazil.
Brasil, 2015Co-Authors: Pagnani Kjr, Castro Afp, Gottschalk M, Wd Silveira, Nakazato GAbstract:Streptococcus suds infection in swine is common in all countries where hog production is well developed. This infection has been associated with bronchopneumonia, meningitis, arthritis, pericarditis, myocarditis, endocarditis, fibrinous polyserositis, septicaemia, rhinitis, and abortion. Streptococcus suis has also been described as a pathogen for ruminants and humans. In Brazil there are several clinical evidences about the existence of S. suds disease in pigs affecting more than 50 0 of farms in States of Sao Paulo, Minas Gerais and Parana. In the present research 51 strains of S. suis isolated from piggeries of the States of Sao Paulo, Minas Gerais and Parana were collected from different pathologies such as septicaemia, meningitis, arthritis and pneumonia and been recovered either in pure culture or as the predominant organism from porcine tissues. Culture of specimens was carried out on 5% bovine blood agar plates incubated at 37degreesC for 24 hr. For the biochemical identification the a-hemolytic colonies of all capsulated isolates were submitted to various conventional Tests, such as hydrolysis of arginine, Voges-Proskauer Test, and production of acid from various carbohydrates (inulin, salicin, trehalose, lactose, sucrose, sorbitol, mannitol and glycerol). The strains were also Tested for their ability to grow in the presence of 6,5% NaCl and for the amylase production. In addition strains were Tested by Api Strep 20 to confirm the identification of S.suis. For capsular typing only capsulated strains were typed by co-agglutination Test, using antisera raised in rabbits against all reference strains from serotypes 1 to 8. Strains belonging to other serotypes were also typed. The co-agglutination was used for serotyping and the capsular reaction Test was carried out for measuring the potency of the prepared antisera. From the total of 51 examined strains the following results were obtained, with regard to serotyping: 30 (58,8%) were serotype 2, 11 (21,6%) were serotype 3, seven (13,72%) were serotype 7, two (3,92%) were serotype 1 and one strain belonged to serotype 14 (1,96%). As far as we are concerned, this is the first report on the isolation of a large number of S. suis strains in Brazil, from cases of illness caused by this bacterium among piglets. Also it was carried out serotyping of the isolates, showing a high prevalence of serotype 2, as compared to other known serotypes of S. suis.2211
-
Serotyping Of Streptococcus Suis Strains Isolated From Pigs In The States Of São Paulo, Minas Gerais E Paraná, Brazil [sorotipagem De Amostras De Streptococcus Suis Isoladas De Suínos Em Granjas Dos Estados De Sã Paulo, Minas Gerais E Paraná]
2015Co-Authors: Pagnani K.j.r., Gottschalk M, Pestana Castro A.f., Silveira W.d., Nakazato GAbstract:Streptococcus suis infection in swine is common in all countries where hog production is well developed. This infection has been associated with bronchopneumonia, meningitis, arthritis, pericarditis, myocarditis, endocarditis, fibrinous polyserositis, septicaemia, rhinitis, and abortion. Streptococcus suis has also been described as a pathogen for ruminants and humans. In Brazil there are several clinical evidences about the existence of S. suis disease in pigs affecting more than 50% of farms in States of São Paulo, Minas Gerais and Paraná. In the present research 51 strains of S. suis isolated from piggeries of the States of São Paulo, Minas Gerais and Paraná were collected from different pathologies such as septicaemia, meningitis, arthritis and pneumonia and been recovered either in pure culture or as the predominant organism from porcine tissues. Culture of specimens was carried out on 5% bovine blood agar plates incubated at 37°C for 24 hr. For the biochemical identification the a-hemolytic colonies of all capsulated isolates were submitted to various conventional Tests, such as hydrolysis of arginine, Voges-Proskauer Test, and production of acid from various carbohydrates (inulin, salicin, trehalose, lactose, sucrose, sorbitol, mannitol and glycerol). The strains were also Tested for their ability to grow in the presence of 6,5% NaCl and for the amylase production. In addition strains were Tested by Api Strep 20 to confirm the identification of S. suis. For capsular typing only capsulated strains were typed by co-agglutination Test, using antisera raised in rabbits against all reference strains from serotypes 1 to 8. Strains belonging to other serotypes were also typed. The co-agglutination was used for serotyping and the capsular reaction Test was carried out for measuring the potency of the prepared antisera. From the total of 51 examined strains the following results were obtained, with regard to serotyping: 30 (58,8%) were serotype 2, 11 (21,6%) were serotype 3, seven (13,72%) were serotype 7, two (3,92%) were serotype 1 and one strain belonged to serotype 14 (1,96%). As far as we are concerned, this is the first report on the isolation of a large number of S. suis strains in Brazil, from cases of illness caused by this bacterium among piglets. Also it was carried out serotyping of the isolates, showing a high prevalence of serotype 2, as compared to other known serotypes of S. suis
-
Serotyping Of Streptococcus Suis Strains Isolated From Pigs In The States Of São Paulo, Minas Gerais E Paraná, Brazil [sorotipagem De Amostras De Streptococcus Suis Isoladas De Suínos Em Granjas Dos Estados De Sã Paulo, Minas Gerais E Paraná]
2015Co-Authors: Pagnani K.j.r., Gottschalk M, Pestana Castro A.f., Silveira W.d., Nakazato GAbstract:Streptococcus suis infection in swine is common in all countries where hog production is well developed. This infection has been associated with bronchopneumonia, meningitis, arthritis, pericarditis, myocarditis, endocarditis, fibrinous polyserositis, septicaemia, rhinitis, and abortion. Streptococcus suis has also been described as a pathogen for ruminants and humans. In Brazil there are several clinical evidences about the existence of S. suis disease in pigs affecting more than 50% of farms in States of São Paulo, Minas Gerais and Paraná. In the present research 51 strains of S. suis isolated from piggeries of the States of São Paulo, Minas Gerais and Paraná were collected from different pathologies such as septicaemia, meningitis, arthritis and pneumonia and been recovered either in pure culture or as the predominant organism from porcine tissues. Culture of specimens was carried out on 5% bovine blood agar plates incubated at 37°C for 24 hr. For the biochemical identification the a-hemolytic colonies of all capsulated isolates were submitted to various conventional Tests, such as hydrolysis of arginine, Voges-Proskauer Test, and production of acid from various carbohydrates (inulin, salicin, trehalose, lactose, sucrose, sorbitol, mannitol and glycerol). The strains were also Tested for their ability to grow in the presence of 6,5% NaCl and for the amylase production. In addition strains were Tested by Api Strep 20 to confirm the identification of S. suis. For capsular typing only capsulated strains were typed by co-agglutination Test, using antisera raised in rabbits against all reference strains from serotypes 1 to 8. Strains belonging to other serotypes were also typed. The co-agglutination was used for serotyping and the capsular reaction Test was carried out for measuring the potency of the prepared antisera. From the total of 51 examined strains the following results were obtained, with regard to serotyping: 30 (58,8%) were serotype 2, 11 (21,6%) were serotype 3, seven (13,72%) were serotype 7, two (3,92%) were serotype 1 and one strain belonged to serotype 14 (1,96%). As far as we are concerned, this is the first report on the isolation of a large number of S. suis strains in Brazil, from cases of illness caused by this bacterium among piglets. Also it was carried out serotyping of the isolates, showing a high prevalence of serotype 2, as compared to other known serotypes of S. suis.22115Arends, J.P., Zanen, H.C., Meningitis caused by Streptococcus suis in humans (1988) Rev. Infect. Dis., 10, pp. 131-137De Moor, C.E., (1963) Septicemic Infections in Pigs Caused by Haemolytic Streptococci of New Lancefield Groups Designated R, S and T. Antonie van Leeuvenhoek, 29, pp. 272-280Field, H.I., Buntain, D., Done, J.T., Studies on piglet mortality. I. Streptococcal meningitis and arthritis (1954) Vet. Rec., 66, pp. 453-455Galina, L., Collins, J.E., Pijoan, C., Porcine Streptococcus suis in Minnesota (1992) J. Vet. Diagn. Invest., 4, pp. 195-196Gottschalk, M., Segura, M., The pathogenesis of the meningitis caused by Streptococcus suis: The unresolved questions (2000) Vet. Microbiol., 76, pp. 259-272Gottschalk, M., Higgins, R., Jacques, M., Mittal, K.R., Henrichsen, J., Description of 14 new capsular types of Streptococcus suis (1989) J. Clin. Microbiol., 27, pp. 2633-2635Gottschalk, M., Higgins, R., Jacqnes, M., Beaudin, M., Henrichsen, J., Characterization of six new capsular types (23 through 28) of Streptococcus suis (1991) J. Clin. Microbiol., 29, pp. 2590-2594Higgins, R., Gottschalk, M., An update on Streptococcus suis identification (1990) J. Vet. Diagn. Invest., 2, pp. 249-252Higgins, R., Gottschalk, M., Distribution of Streptococcus suis capsular types in 1995 (1996) Can. Vet. J., 37, p. 242Higgins, R., Gottschalk, M., Boudreau, M., Lebrun, A., Henrichsen, J., Description of six new capsular types (29-34) of Streptococcus suis (1995) J. Vet. Invest., 7, pp. 405-406Hogg, A., Aamass, S.F., Hoffman, L.J., Wu, C.C., Clark, L.K., A survey of Streptococcus suis isolations by serotype and tissue of origin (1996) Proc. Am. Assoc. Swine Pract., pp. 79-81Hommez, J., Wullepit, J., Cassimon, P., Ceyssens, K., Devries, L.A., Streptococcus suis and other streptococcal species as a cause of extramammary infection in ruminants (1988) Vet. Rec., 123, pp. 626-627Jansen, E.J., Van Dorssen, C.A., Meningoencephalitis bij varkens door streptococcen (1951) Tijdschr. Diergeneeskd., 76, pp. 815-832Kataoka, Y., Sugimoto, C., Nakazawa, M., Morozumi, T., Kashiwazaki, M., The epidemiological studies of Streptococcus suis infections in Japan from 1987 to 1991 (1993) J. Vet. Med., 55, pp. 623-626Kilper-Balz, R., Schleifer, K.H., Streptococcus suis sp. nov.nom. rev. (1987) Int. J. Syst. Bacteriol., 37, pp. 160-162Mittal, K., Higgins, R., Larivière, S., Leblanc, D., A 2-mercaptoetanol tube agglutination Test for diagnosis of Haemophilus pleuropneumoniae infection in pigs (1984) Am J. Vet. Res., 45, pp. 715-719Perch, B., Pedersen, K.B., Henrichse, J., Serology of capsulated streptococci pathogenic for pigs: Six new serotypes of Streptococcus suis (1983) J. Clin. Microbiol., 17, pp. 993-996Prieto, C., Garcia, F.J., Suarez, P., Imaz, M., Castro, J.M., Biochemical traits and antimicrobial susceptibility of Streptococcus suis isolated from slaughtered pigs (1994) J. Vet. Med. B, 41, pp. 608-617Reams, R.Y., Harrington, D.D., Glickman, L.T., Thacker, H.L., Bowersock, T.L., Multiple serotypes and strains of Streptococcus suis in naturally infected swine herds (1996) J. Vet. Diagn. Invest., 8, pp. 119-121Windsor, R.S., Elliot, S.D., Streptococcal infection in young pigs. IV. An outbreak of streptococcal meningitis in weaned pigs (1975) J. Hyg., 75, pp. 69-78Zanen, H.C., Engel, H.W.B., Porcine streptococci causing meningitis and septicaemia in man (1975) Lancet, 7919, pp. 1286-128
Pagnani K.j.r. - One of the best experts on this subject based on the ideXlab platform.
-
Serotyping Of Streptococcus Suis Strains Isolated From Pigs In The States Of São Paulo, Minas Gerais E Paraná, Brazil [sorotipagem De Amostras De Streptococcus Suis Isoladas De Suínos Em Granjas Dos Estados De Sã Paulo, Minas Gerais E Paraná]
2015Co-Authors: Pagnani K.j.r., Gottschalk M, Pestana Castro A.f., Silveira W.d., Nakazato GAbstract:Streptococcus suis infection in swine is common in all countries where hog production is well developed. This infection has been associated with bronchopneumonia, meningitis, arthritis, pericarditis, myocarditis, endocarditis, fibrinous polyserositis, septicaemia, rhinitis, and abortion. Streptococcus suis has also been described as a pathogen for ruminants and humans. In Brazil there are several clinical evidences about the existence of S. suis disease in pigs affecting more than 50% of farms in States of São Paulo, Minas Gerais and Paraná. In the present research 51 strains of S. suis isolated from piggeries of the States of São Paulo, Minas Gerais and Paraná were collected from different pathologies such as septicaemia, meningitis, arthritis and pneumonia and been recovered either in pure culture or as the predominant organism from porcine tissues. Culture of specimens was carried out on 5% bovine blood agar plates incubated at 37°C for 24 hr. For the biochemical identification the a-hemolytic colonies of all capsulated isolates were submitted to various conventional Tests, such as hydrolysis of arginine, Voges-Proskauer Test, and production of acid from various carbohydrates (inulin, salicin, trehalose, lactose, sucrose, sorbitol, mannitol and glycerol). The strains were also Tested for their ability to grow in the presence of 6,5% NaCl and for the amylase production. In addition strains were Tested by Api Strep 20 to confirm the identification of S. suis. For capsular typing only capsulated strains were typed by co-agglutination Test, using antisera raised in rabbits against all reference strains from serotypes 1 to 8. Strains belonging to other serotypes were also typed. The co-agglutination was used for serotyping and the capsular reaction Test was carried out for measuring the potency of the prepared antisera. From the total of 51 examined strains the following results were obtained, with regard to serotyping: 30 (58,8%) were serotype 2, 11 (21,6%) were serotype 3, seven (13,72%) were serotype 7, two (3,92%) were serotype 1 and one strain belonged to serotype 14 (1,96%). As far as we are concerned, this is the first report on the isolation of a large number of S. suis strains in Brazil, from cases of illness caused by this bacterium among piglets. Also it was carried out serotyping of the isolates, showing a high prevalence of serotype 2, as compared to other known serotypes of S. suis
-
Serotyping Of Streptococcus Suis Strains Isolated From Pigs In The States Of São Paulo, Minas Gerais E Paraná, Brazil [sorotipagem De Amostras De Streptococcus Suis Isoladas De Suínos Em Granjas Dos Estados De Sã Paulo, Minas Gerais E Paraná]
2015Co-Authors: Pagnani K.j.r., Gottschalk M, Pestana Castro A.f., Silveira W.d., Nakazato GAbstract:Streptococcus suis infection in swine is common in all countries where hog production is well developed. This infection has been associated with bronchopneumonia, meningitis, arthritis, pericarditis, myocarditis, endocarditis, fibrinous polyserositis, septicaemia, rhinitis, and abortion. Streptococcus suis has also been described as a pathogen for ruminants and humans. In Brazil there are several clinical evidences about the existence of S. suis disease in pigs affecting more than 50% of farms in States of São Paulo, Minas Gerais and Paraná. In the present research 51 strains of S. suis isolated from piggeries of the States of São Paulo, Minas Gerais and Paraná were collected from different pathologies such as septicaemia, meningitis, arthritis and pneumonia and been recovered either in pure culture or as the predominant organism from porcine tissues. Culture of specimens was carried out on 5% bovine blood agar plates incubated at 37°C for 24 hr. For the biochemical identification the a-hemolytic colonies of all capsulated isolates were submitted to various conventional Tests, such as hydrolysis of arginine, Voges-Proskauer Test, and production of acid from various carbohydrates (inulin, salicin, trehalose, lactose, sucrose, sorbitol, mannitol and glycerol). The strains were also Tested for their ability to grow in the presence of 6,5% NaCl and for the amylase production. In addition strains were Tested by Api Strep 20 to confirm the identification of S. suis. For capsular typing only capsulated strains were typed by co-agglutination Test, using antisera raised in rabbits against all reference strains from serotypes 1 to 8. Strains belonging to other serotypes were also typed. The co-agglutination was used for serotyping and the capsular reaction Test was carried out for measuring the potency of the prepared antisera. From the total of 51 examined strains the following results were obtained, with regard to serotyping: 30 (58,8%) were serotype 2, 11 (21,6%) were serotype 3, seven (13,72%) were serotype 7, two (3,92%) were serotype 1 and one strain belonged to serotype 14 (1,96%). As far as we are concerned, this is the first report on the isolation of a large number of S. suis strains in Brazil, from cases of illness caused by this bacterium among piglets. Also it was carried out serotyping of the isolates, showing a high prevalence of serotype 2, as compared to other known serotypes of S. suis.22115Arends, J.P., Zanen, H.C., Meningitis caused by Streptococcus suis in humans (1988) Rev. Infect. Dis., 10, pp. 131-137De Moor, C.E., (1963) Septicemic Infections in Pigs Caused by Haemolytic Streptococci of New Lancefield Groups Designated R, S and T. Antonie van Leeuvenhoek, 29, pp. 272-280Field, H.I., Buntain, D., Done, J.T., Studies on piglet mortality. I. Streptococcal meningitis and arthritis (1954) Vet. Rec., 66, pp. 453-455Galina, L., Collins, J.E., Pijoan, C., Porcine Streptococcus suis in Minnesota (1992) J. Vet. Diagn. Invest., 4, pp. 195-196Gottschalk, M., Segura, M., The pathogenesis of the meningitis caused by Streptococcus suis: The unresolved questions (2000) Vet. Microbiol., 76, pp. 259-272Gottschalk, M., Higgins, R., Jacques, M., Mittal, K.R., Henrichsen, J., Description of 14 new capsular types of Streptococcus suis (1989) J. Clin. Microbiol., 27, pp. 2633-2635Gottschalk, M., Higgins, R., Jacqnes, M., Beaudin, M., Henrichsen, J., Characterization of six new capsular types (23 through 28) of Streptococcus suis (1991) J. Clin. Microbiol., 29, pp. 2590-2594Higgins, R., Gottschalk, M., An update on Streptococcus suis identification (1990) J. Vet. Diagn. Invest., 2, pp. 249-252Higgins, R., Gottschalk, M., Distribution of Streptococcus suis capsular types in 1995 (1996) Can. Vet. J., 37, p. 242Higgins, R., Gottschalk, M., Boudreau, M., Lebrun, A., Henrichsen, J., Description of six new capsular types (29-34) of Streptococcus suis (1995) J. Vet. Invest., 7, pp. 405-406Hogg, A., Aamass, S.F., Hoffman, L.J., Wu, C.C., Clark, L.K., A survey of Streptococcus suis isolations by serotype and tissue of origin (1996) Proc. Am. Assoc. Swine Pract., pp. 79-81Hommez, J., Wullepit, J., Cassimon, P., Ceyssens, K., Devries, L.A., Streptococcus suis and other streptococcal species as a cause of extramammary infection in ruminants (1988) Vet. Rec., 123, pp. 626-627Jansen, E.J., Van Dorssen, C.A., Meningoencephalitis bij varkens door streptococcen (1951) Tijdschr. Diergeneeskd., 76, pp. 815-832Kataoka, Y., Sugimoto, C., Nakazawa, M., Morozumi, T., Kashiwazaki, M., The epidemiological studies of Streptococcus suis infections in Japan from 1987 to 1991 (1993) J. Vet. Med., 55, pp. 623-626Kilper-Balz, R., Schleifer, K.H., Streptococcus suis sp. nov.nom. rev. (1987) Int. J. Syst. Bacteriol., 37, pp. 160-162Mittal, K., Higgins, R., Larivière, S., Leblanc, D., A 2-mercaptoetanol tube agglutination Test for diagnosis of Haemophilus pleuropneumoniae infection in pigs (1984) Am J. Vet. Res., 45, pp. 715-719Perch, B., Pedersen, K.B., Henrichse, J., Serology of capsulated streptococci pathogenic for pigs: Six new serotypes of Streptococcus suis (1983) J. Clin. Microbiol., 17, pp. 993-996Prieto, C., Garcia, F.J., Suarez, P., Imaz, M., Castro, J.M., Biochemical traits and antimicrobial susceptibility of Streptococcus suis isolated from slaughtered pigs (1994) J. Vet. Med. B, 41, pp. 608-617Reams, R.Y., Harrington, D.D., Glickman, L.T., Thacker, H.L., Bowersock, T.L., Multiple serotypes and strains of Streptococcus suis in naturally infected swine herds (1996) J. Vet. Diagn. Invest., 8, pp. 119-121Windsor, R.S., Elliot, S.D., Streptococcal infection in young pigs. IV. An outbreak of streptococcal meningitis in weaned pigs (1975) J. Hyg., 75, pp. 69-78Zanen, H.C., Engel, H.W.B., Porcine streptococci causing meningitis and septicaemia in man (1975) Lancet, 7919, pp. 1286-128
Gottschalk M - One of the best experts on this subject based on the ideXlab platform.
-
Serotyping of Streptococcus suis strains isolated from pigs in the states of Sao Paulo, Minas Gerais and Parana, Brazil.
Brasil, 2015Co-Authors: Pagnani Kjr, Castro Afp, Gottschalk M, Wd Silveira, Nakazato GAbstract:Streptococcus suds infection in swine is common in all countries where hog production is well developed. This infection has been associated with bronchopneumonia, meningitis, arthritis, pericarditis, myocarditis, endocarditis, fibrinous polyserositis, septicaemia, rhinitis, and abortion. Streptococcus suis has also been described as a pathogen for ruminants and humans. In Brazil there are several clinical evidences about the existence of S. suds disease in pigs affecting more than 50 0 of farms in States of Sao Paulo, Minas Gerais and Parana. In the present research 51 strains of S. suis isolated from piggeries of the States of Sao Paulo, Minas Gerais and Parana were collected from different pathologies such as septicaemia, meningitis, arthritis and pneumonia and been recovered either in pure culture or as the predominant organism from porcine tissues. Culture of specimens was carried out on 5% bovine blood agar plates incubated at 37degreesC for 24 hr. For the biochemical identification the a-hemolytic colonies of all capsulated isolates were submitted to various conventional Tests, such as hydrolysis of arginine, Voges-Proskauer Test, and production of acid from various carbohydrates (inulin, salicin, trehalose, lactose, sucrose, sorbitol, mannitol and glycerol). The strains were also Tested for their ability to grow in the presence of 6,5% NaCl and for the amylase production. In addition strains were Tested by Api Strep 20 to confirm the identification of S.suis. For capsular typing only capsulated strains were typed by co-agglutination Test, using antisera raised in rabbits against all reference strains from serotypes 1 to 8. Strains belonging to other serotypes were also typed. The co-agglutination was used for serotyping and the capsular reaction Test was carried out for measuring the potency of the prepared antisera. From the total of 51 examined strains the following results were obtained, with regard to serotyping: 30 (58,8%) were serotype 2, 11 (21,6%) were serotype 3, seven (13,72%) were serotype 7, two (3,92%) were serotype 1 and one strain belonged to serotype 14 (1,96%). As far as we are concerned, this is the first report on the isolation of a large number of S. suis strains in Brazil, from cases of illness caused by this bacterium among piglets. Also it was carried out serotyping of the isolates, showing a high prevalence of serotype 2, as compared to other known serotypes of S. suis.2211
-
Serotyping Of Streptococcus Suis Strains Isolated From Pigs In The States Of São Paulo, Minas Gerais E Paraná, Brazil [sorotipagem De Amostras De Streptococcus Suis Isoladas De Suínos Em Granjas Dos Estados De Sã Paulo, Minas Gerais E Paraná]
2015Co-Authors: Pagnani K.j.r., Gottschalk M, Pestana Castro A.f., Silveira W.d., Nakazato GAbstract:Streptococcus suis infection in swine is common in all countries where hog production is well developed. This infection has been associated with bronchopneumonia, meningitis, arthritis, pericarditis, myocarditis, endocarditis, fibrinous polyserositis, septicaemia, rhinitis, and abortion. Streptococcus suis has also been described as a pathogen for ruminants and humans. In Brazil there are several clinical evidences about the existence of S. suis disease in pigs affecting more than 50% of farms in States of São Paulo, Minas Gerais and Paraná. In the present research 51 strains of S. suis isolated from piggeries of the States of São Paulo, Minas Gerais and Paraná were collected from different pathologies such as septicaemia, meningitis, arthritis and pneumonia and been recovered either in pure culture or as the predominant organism from porcine tissues. Culture of specimens was carried out on 5% bovine blood agar plates incubated at 37°C for 24 hr. For the biochemical identification the a-hemolytic colonies of all capsulated isolates were submitted to various conventional Tests, such as hydrolysis of arginine, Voges-Proskauer Test, and production of acid from various carbohydrates (inulin, salicin, trehalose, lactose, sucrose, sorbitol, mannitol and glycerol). The strains were also Tested for their ability to grow in the presence of 6,5% NaCl and for the amylase production. In addition strains were Tested by Api Strep 20 to confirm the identification of S. suis. For capsular typing only capsulated strains were typed by co-agglutination Test, using antisera raised in rabbits against all reference strains from serotypes 1 to 8. Strains belonging to other serotypes were also typed. The co-agglutination was used for serotyping and the capsular reaction Test was carried out for measuring the potency of the prepared antisera. From the total of 51 examined strains the following results were obtained, with regard to serotyping: 30 (58,8%) were serotype 2, 11 (21,6%) were serotype 3, seven (13,72%) were serotype 7, two (3,92%) were serotype 1 and one strain belonged to serotype 14 (1,96%). As far as we are concerned, this is the first report on the isolation of a large number of S. suis strains in Brazil, from cases of illness caused by this bacterium among piglets. Also it was carried out serotyping of the isolates, showing a high prevalence of serotype 2, as compared to other known serotypes of S. suis
-
Serotyping Of Streptococcus Suis Strains Isolated From Pigs In The States Of São Paulo, Minas Gerais E Paraná, Brazil [sorotipagem De Amostras De Streptococcus Suis Isoladas De Suínos Em Granjas Dos Estados De Sã Paulo, Minas Gerais E Paraná]
2015Co-Authors: Pagnani K.j.r., Gottschalk M, Pestana Castro A.f., Silveira W.d., Nakazato GAbstract:Streptococcus suis infection in swine is common in all countries where hog production is well developed. This infection has been associated with bronchopneumonia, meningitis, arthritis, pericarditis, myocarditis, endocarditis, fibrinous polyserositis, septicaemia, rhinitis, and abortion. Streptococcus suis has also been described as a pathogen for ruminants and humans. In Brazil there are several clinical evidences about the existence of S. suis disease in pigs affecting more than 50% of farms in States of São Paulo, Minas Gerais and Paraná. In the present research 51 strains of S. suis isolated from piggeries of the States of São Paulo, Minas Gerais and Paraná were collected from different pathologies such as septicaemia, meningitis, arthritis and pneumonia and been recovered either in pure culture or as the predominant organism from porcine tissues. Culture of specimens was carried out on 5% bovine blood agar plates incubated at 37°C for 24 hr. For the biochemical identification the a-hemolytic colonies of all capsulated isolates were submitted to various conventional Tests, such as hydrolysis of arginine, Voges-Proskauer Test, and production of acid from various carbohydrates (inulin, salicin, trehalose, lactose, sucrose, sorbitol, mannitol and glycerol). The strains were also Tested for their ability to grow in the presence of 6,5% NaCl and for the amylase production. In addition strains were Tested by Api Strep 20 to confirm the identification of S. suis. For capsular typing only capsulated strains were typed by co-agglutination Test, using antisera raised in rabbits against all reference strains from serotypes 1 to 8. Strains belonging to other serotypes were also typed. The co-agglutination was used for serotyping and the capsular reaction Test was carried out for measuring the potency of the prepared antisera. From the total of 51 examined strains the following results were obtained, with regard to serotyping: 30 (58,8%) were serotype 2, 11 (21,6%) were serotype 3, seven (13,72%) were serotype 7, two (3,92%) were serotype 1 and one strain belonged to serotype 14 (1,96%). As far as we are concerned, this is the first report on the isolation of a large number of S. suis strains in Brazil, from cases of illness caused by this bacterium among piglets. Also it was carried out serotyping of the isolates, showing a high prevalence of serotype 2, as compared to other known serotypes of S. suis.22115Arends, J.P., Zanen, H.C., Meningitis caused by Streptococcus suis in humans (1988) Rev. Infect. Dis., 10, pp. 131-137De Moor, C.E., (1963) Septicemic Infections in Pigs Caused by Haemolytic Streptococci of New Lancefield Groups Designated R, S and T. Antonie van Leeuvenhoek, 29, pp. 272-280Field, H.I., Buntain, D., Done, J.T., Studies on piglet mortality. I. Streptococcal meningitis and arthritis (1954) Vet. Rec., 66, pp. 453-455Galina, L., Collins, J.E., Pijoan, C., Porcine Streptococcus suis in Minnesota (1992) J. Vet. Diagn. Invest., 4, pp. 195-196Gottschalk, M., Segura, M., The pathogenesis of the meningitis caused by Streptococcus suis: The unresolved questions (2000) Vet. Microbiol., 76, pp. 259-272Gottschalk, M., Higgins, R., Jacques, M., Mittal, K.R., Henrichsen, J., Description of 14 new capsular types of Streptococcus suis (1989) J. Clin. Microbiol., 27, pp. 2633-2635Gottschalk, M., Higgins, R., Jacqnes, M., Beaudin, M., Henrichsen, J., Characterization of six new capsular types (23 through 28) of Streptococcus suis (1991) J. Clin. Microbiol., 29, pp. 2590-2594Higgins, R., Gottschalk, M., An update on Streptococcus suis identification (1990) J. Vet. Diagn. Invest., 2, pp. 249-252Higgins, R., Gottschalk, M., Distribution of Streptococcus suis capsular types in 1995 (1996) Can. Vet. J., 37, p. 242Higgins, R., Gottschalk, M., Boudreau, M., Lebrun, A., Henrichsen, J., Description of six new capsular types (29-34) of Streptococcus suis (1995) J. Vet. Invest., 7, pp. 405-406Hogg, A., Aamass, S.F., Hoffman, L.J., Wu, C.C., Clark, L.K., A survey of Streptococcus suis isolations by serotype and tissue of origin (1996) Proc. Am. Assoc. Swine Pract., pp. 79-81Hommez, J., Wullepit, J., Cassimon, P., Ceyssens, K., Devries, L.A., Streptococcus suis and other streptococcal species as a cause of extramammary infection in ruminants (1988) Vet. Rec., 123, pp. 626-627Jansen, E.J., Van Dorssen, C.A., Meningoencephalitis bij varkens door streptococcen (1951) Tijdschr. Diergeneeskd., 76, pp. 815-832Kataoka, Y., Sugimoto, C., Nakazawa, M., Morozumi, T., Kashiwazaki, M., The epidemiological studies of Streptococcus suis infections in Japan from 1987 to 1991 (1993) J. Vet. Med., 55, pp. 623-626Kilper-Balz, R., Schleifer, K.H., Streptococcus suis sp. nov.nom. rev. (1987) Int. J. Syst. Bacteriol., 37, pp. 160-162Mittal, K., Higgins, R., Larivière, S., Leblanc, D., A 2-mercaptoetanol tube agglutination Test for diagnosis of Haemophilus pleuropneumoniae infection in pigs (1984) Am J. Vet. Res., 45, pp. 715-719Perch, B., Pedersen, K.B., Henrichse, J., Serology of capsulated streptococci pathogenic for pigs: Six new serotypes of Streptococcus suis (1983) J. Clin. Microbiol., 17, pp. 993-996Prieto, C., Garcia, F.J., Suarez, P., Imaz, M., Castro, J.M., Biochemical traits and antimicrobial susceptibility of Streptococcus suis isolated from slaughtered pigs (1994) J. Vet. Med. B, 41, pp. 608-617Reams, R.Y., Harrington, D.D., Glickman, L.T., Thacker, H.L., Bowersock, T.L., Multiple serotypes and strains of Streptococcus suis in naturally infected swine herds (1996) J. Vet. Diagn. Invest., 8, pp. 119-121Windsor, R.S., Elliot, S.D., Streptococcal infection in young pigs. IV. An outbreak of streptococcal meningitis in weaned pigs (1975) J. Hyg., 75, pp. 69-78Zanen, H.C., Engel, H.W.B., Porcine streptococci causing meningitis and septicaemia in man (1975) Lancet, 7919, pp. 1286-128
Kwokyung Yuen - One of the best experts on this subject based on the ideXlab platform.
-
streptococcus hongkongensis sp nov isolated from a patient with an infected puncture wound and from a marine flatfish
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Shirly O T Curreem, Ami M Y Fung, Kwokyung YuenAbstract:A bacterium, HKU30T, was isolated from the infected tissue of a patient with wound infection after puncture by a fish fin. Cells are facultative anaerobic, non-spore-forming, non-motile, Gram-positive cocci arranged in chains. Colonies were non-haemolytic. The strain was catalase, oxidase, urease and Voges–Proskauer Test negative. It reacted with Lancefield’s group G antisera and was resistant to optochin. It grew on bile aesculin agar and in 5 % NaCl. It was unidentified by three commercial identification systems. 16S rRNA gene sequence analysis indicated that the bacterium shared 98.2, 97.7, 97.4 and 97.1 % nucleotide identities with Streptococcus iniae , Streptococcus pseudoporcinus , Streptococcus parauberis and Streptococcus uberis , respectively. The DNA G+C content was 35.6±0.9 mol% (mean±sd). In view of the occupational exposure of the patient, an epidemiological study was performed to isolate the bacterium from marine fish. Two strains, with similar phenotypic and genotypic characteristics to those of HKU30T, were isolated from a three-lined tongue sole (Cynoglossus abbreviatus) and an olive flounder (Paralichthys olivaceus) respectively. Phylogenetic analysis of four additional housekeeping genes, groEL, gyrB, sodA and rpoB, showed that the three isolates formed a distinct branch among known species of the genus Streptococcus , being most closely related to S. parauberis (CCUG 39954T). DNA–DNA hybridization demonstrated ≤53.8 % DNA relatedness between the three isolates and related species of the genus Streptococcus . A novel species, Streptococcus hongkongensis sp. nov., is proposed. The type strain is HKU30T ( = DSM 26014T = CECT 8154T).
-
Streptococcus sinensis sp. nov., a Novel Species Isolated from a Patient with Infective Endocarditis
Journal of clinical microbiology, 2002Co-Authors: Patrick C. Y. Woo, Dorothy M. W. Tam, Kit-wah Leung, Susanna K. P. Lau, Jade L. L. Teng, Michelle K.m. Wong, Kwokyung YuenAbstract:A bacterium was isolated from the blood culture of a patient with infective endocarditis. The cells were facultative anaerobic, nonsporulating, gram-positive cocci arranged in chains. The bacterium grows on sheep blood agar as alpha-hemolytic, gray colonies of 0.5 to 1 mm in diameter after 24 h of incubation at 37°C in ambient air. Growth also occurs in 10 or 40% bile and on bile esculin agar but not in 6% NaCl. No enhancement of growth is observed in 5% CO2. It is nongroupable with Lancefield groups A, B, C, D, F, or G antisera and is resistant to optochin and bacitracin. The organism is aflagellated and is nonmotile at both 25 and 37°C. It is Voges-Proskauer Test positive. It produces leucine arylamidase and β-glucosidase but not catalase, urease, lysine decarboxylase, or ornithine decarboxylase. It hydrolyzes esculin and arginine. It utilizes glucose, lactose, salicin, sucrose, pullulan, trehalose, cellobiose, hemicellulase, mannose, maltose, and starch. 16S rRNA gene sequencing showed that there were 3.6, 3.7, 4.3, 4.7, and 5.9% differences between the 16S rRNA gene sequence of the bacterium and those of Streptococcus gordonii, Streptococcus intermedius, Streptococcus constellatus, Streptococcus sanguis, and Streptococcus anginosus, respectively. The G+C content of it (mean ± standard deviation) was 53.0% ± 2.9%. Based on phylogenetic affiliation, it belongs to the mitis or anginosus group of Streptococcus. For these reasons a new species, Streptococcus sinensis sp. nov., is proposed, for which HKU4 is the type strain. Further studies should be performed to ascertain the potential of this bacterium to become an emerging cause of infective endocarditis.
Vendruscolo, Eliane Cristina Gruzka - One of the best experts on this subject based on the ideXlab platform.
-
Ocorrência e caracterização genética de Staphylococcus aureus em amostras de leite de gado com mastite, na equipe de um Hospital Veterinário e em pessoas que trabalham com o gado leiteiro
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia, 2017Co-Authors: Leigue Lucianne, Hilgert, Ayrton Rodrigo, Fiorini Adriana, Santos, Marise Fonseca Dos, Vendruscolo, Eliane Cristina GruzkaAbstract:Staphylococcus aureus is one of the most common microorganisms responsible for high morbidity and mortality in humans and animals. Methicillin-resistant S. aureus are responsible for several outbreaks worldwide and therapeutic arsenal has become scarce. The present investigation verified the epidemiological profile of S. aureus strains isolated from the veterinary hospital staff, from dairy cattle workers and also from milk samples of dairy cattle presenting mastitis. Samples were characterized phenotypically by antibiogram, catalase, and coagulase Tests, and also by Voges-Proskauer Test. The isolated strains were characterized genotypically by specific Polymerase Chain Reaction and Amplified Ribosomal DNA Restriction Analysis (ARDRA). From the 218 isolated strains, 27 were identified as S. aureus (12%), four of them were resistant to oxacillin and two of them were classified as MRSA (Methicillin-resistant S. aureus). The prevalence of isolated strains among animal personnel care was low (2%) but all MRSA isolates were found among the clinical staff. Results of ARDRA pointed out that S. aureus strains isolated from different animal care personnel were grouped in the same cluster when HindIII and HinfII restriction enzymes were used. When ARDRA was performed with HaeIII enzyme, the formation of two clusters was observed, but the isolated strains were not correlated. The prevalence of S. aureus strains isolated was higher in clinical staff and the biochemical and molecular assays of them presented 100% of correlation.Staphylococcus aureus está entre os microrganismos que apresentam as maiores taxas de morbidade e mortalidade em seres humanos e animais. Linhagens de S. aureus resistentes a meticilina podem causar surtos de infecção em todo o mundo, o que contribui para a escassez de arsenal terapêutico. Este trabalho analisou o perfil epidemiológico de estirpes de S. aureus isoladas de pessoas que trabalham em contato com animais em um hospital veterinário com gado leiteiro e também em amostras de leite de vacas acometidas por mastite. As estirpes de S. aureus isoladas foram caracterizadas fenotipicamente por meio de antibiograma, Testes de catalase e coagulase, e pelo Teste de Voges-Proskauer. As amostras também foram caracterizadas genotipicamente pela técnica de Analise de Restricao de DNA Ribossomico Amplificado (ARDRA-PCR). Das 218 estirpes isoladas, 27 foram identificados como S. aureus (12%). Entre essas, quatro estirpes foram resistentes a oxacilina e duas classificadas como SARM (S. aureus resistente a meticilina). A ocorrência de estirpes de S.aureus isoladas entre o pessoal que trabalha em contato com os animais foi baixa (2%), mas estirpes identificadas como SARM foram encontradas na equipe clínica. As análises de ARDRA realizadas com as enzimas de restrição HindIII e HinfII demonstraram que S. aureus isolados de diferentes indivíduos que trabalhavam com animais foram agrupados no mesmo cluster. Quando a ARDRA foi realizada com HaeIII foi observada formação de dois grupos, mas os isolados não se correlacionaram. Conclusão: a ocorrência de estirpes de S. aureus isoladas foi maior na equipe clínica, apresentando também correlação de 100% nos ensaios bioquímicos e moleculares
