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Katsushige Sato - One of the best experts on this subject based on the ideXlab platform.
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Functiogenesis of the embryonic central nervous system revealed by optical recording with a Voltage-Sensitive Dye
The Journal of Physiological Sciences, 2017Co-Authors: Katsushige Sato, Yoko Momose-satoAbstract:Clarification of the functiogenesis of the embryonic central nervous system (CNS) has long been problematic, because conventional electrophysiological techniques have several limitations. First, early embryonic neurons are small and fragile, and the application of microelectrodes is challenging. Second, the simultaneous monitoring of electrical activity from multiple sites is limited, and as a consequence, spatiotemporal response patterns of neural networks cannot be assessed. We have applied multiple-site optical recording with a Voltage-Sensitive Dye to the embryonic CNS and paved a new way to analyze the functiogenesis of the CNS. In this review, we discuss key points of optical recording in the embryonic CNS and introduce recent progress in optical investigations on the embryonic CNS with special emphasis on the development of the chick olfactory system. The studies clearly demonstrate the usefulness of Voltage-Sensitive Dye recording as a powerful tool for elucidating the functional organization of the vertebrate embryonic CNS.
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Voltage-Sensitive Dye imaging during functional development of the embryonic nervous system: a brief review with special thanks to Professor Larry Cohen.
Neurophotonics, 2015Co-Authors: Yoko Momose-sato, Katsushige SatoAbstract:Investigating the developmental organization of the embryonic nervous system is one of the major challenges in the field of neuroscience. Despite their significance, functional studies on the vertebrate embryonic central nervous system (CNS) have been hampered by the technical limitations associated with conventional electrophysiological methods. The advent of optical techniques using Voltage-Sensitive Dyes, which were developed by Dr. Cohen and his colleagues, has enabled electrical activity in living cells to be monitored noninvasively and also facilitated the simultaneous recording of neural responses from multiple regions. Using optical recording techniques, it is now possible to follow the functional organization of the embryonic CNS and image the spatiotemporal dynamics involved in the formation of this neural network. We herein briefly reviewed optical studies on the embryonic CNS with a special emphasis on methodological considerations and the study of neuronal circuit formation, which demonstrates the utility of fast Voltage-Sensitive Dye imaging as a powerful tool for elucidating the functional organization of the embryonic CNS.
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spontaneous depolarization wave in the mouse embryo origin and large scale propagation over the cns identified with voltage sensitive Dye imaging
European Journal of Neuroscience, 2012Co-Authors: Yoko Momosesato, Tomoharu Nakamori, Katsushige SatoAbstract:: Spontaneous embryonic movements, called embryonic motility, are produced by correlated spontaneous activity in the cranial and spinal nerves, which is driven by brainstem and spinal networks. Using optical imaging with a Voltage-Sensitive Dye, we have revealed previously that this correlated activity is a widely propagating wave of neural depolarization, which we termed the depolarization wave. We have observed in the chick and rat embryos that the activity spread over an extensive region of the CNS, including the spinal cord, hindbrain, cerebellum, midbrain and forebrain. One important consideration is whether a depolarization wave with similar characteristics occurs in other species, especially in different mammals. Here, we provide evidence for the existence of the depolarization wave in the mouse embryo by showing that the widely propagating wave appeared independently of the localized spontaneous activity detected previously with Ca(2+) imaging. Furthermore, we mapped the origin of the depolarization wave and revealed that the wave generator moved from the rostral spinal cord to the caudal cord as development proceeded, and was later replaced with mature rhythmogenerators. The present study, together with an accompanying paper that describes pharmacological properties of the mouse depolarization wave, shows that a synchronized wave with common characteristics is expressed in different species, suggesting fundamental roles in neural development.
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functional development of the vagal and glossopharyngeal nerve related nuclei in the embryonic rat brainstem optical mapping with a voltage sensitive Dye
Neuroscience, 2011Co-Authors: Yoko Momosesato, Tomoharu Nakamori, Katsushige SatoAbstract:We investigated functional organization of the vagus nerve (N. X)- and glossopharyngeal nerve (N. IX)-related nuclei in the embryonic rat brainstem and compared their development and spatial distribution patterns, using multiple-site optical recording with a fast Voltage-Sensitive Dye, NK2761. Intact brainstem preparations with N. X and N. IX attached were dissected from E13-E16 rat embryos, and electrical responses evoked by N. X/N. IX stimulation were optically recorded from many loci of the stained preparations. We analyzed optical waveforms and separated fast and slow optical signals corresponding to the antidromic/orthodromic action potentials and the excitatory postsynaptic potentials (EPSPs), respectively. We constructed contour line maps of signal amplitudes and identified motor and sensory nuclei of N. X and N. IX. In the N. X-related motor nucleus (the dorsal motor nucleus of the vagus nerve: DMNV), the fast signals were distributed in multiple-peak patterns, suggesting that the neurons and/or their activity are not distributed uniformly within the motor nuclei at early developmental stages. In the sensory nucleus (the nucleus of the tractus solitarius: NTS), the EPSPs were first detected from E15 in normal physiological solution for both N. X and N. IX. The N. IX-related NTS partially overlapped with the N. X-related NTS, but the peak locations were different between these two nerves. The results obtained in this study suggest that functional organization of the N. X- and N. IX-related nuclei changes dynamically with development in the embryonic rat brainstem.
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Using Voltage-Sensitive Dye recording to image the functional development of neuronal circuits in vertebrate embryos.
Developmental neurobiology, 2008Co-Authors: Joel C. Glover, Katsushige Sato, Yoko Momose-satoAbstract:Recent developments in the design of Voltage-Sensitive Dyes and of recording apparatuses for detecting voltage-dependent changes in the optical properties of such Dyes have established Voltage-Sensitive Dye recording as an important technique for assessing the functional development of neuronal circuits in the brain and spinal cord. Here we discuss general technical issues regarding the recording of Voltage-Sensitive Dye signals and describe studies that have utilized this approach to follow the development of sensory and sensorimotor circuits in the embryonic brain stem. Functional imaging through Voltage-Sensitive Dye recording permits a noninvasive analysis of synaptic development and function at submillisecond temporal resolution in widely distributed circuits. These advantages are particularly valuable in assessing sensorimotor circuit development at early stages when neurons are small and synapses are fragile. © 2008 Wiley Periodicals, Inc. Develop Neurobiol, 2008
Yoko Momose-sato - One of the best experts on this subject based on the ideXlab platform.
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Functiogenesis of the embryonic central nervous system revealed by optical recording with a Voltage-Sensitive Dye
The Journal of Physiological Sciences, 2017Co-Authors: Katsushige Sato, Yoko Momose-satoAbstract:Clarification of the functiogenesis of the embryonic central nervous system (CNS) has long been problematic, because conventional electrophysiological techniques have several limitations. First, early embryonic neurons are small and fragile, and the application of microelectrodes is challenging. Second, the simultaneous monitoring of electrical activity from multiple sites is limited, and as a consequence, spatiotemporal response patterns of neural networks cannot be assessed. We have applied multiple-site optical recording with a Voltage-Sensitive Dye to the embryonic CNS and paved a new way to analyze the functiogenesis of the CNS. In this review, we discuss key points of optical recording in the embryonic CNS and introduce recent progress in optical investigations on the embryonic CNS with special emphasis on the development of the chick olfactory system. The studies clearly demonstrate the usefulness of Voltage-Sensitive Dye recording as a powerful tool for elucidating the functional organization of the vertebrate embryonic CNS.
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Voltage-Sensitive Dye imaging during functional development of the embryonic nervous system: a brief review with special thanks to Professor Larry Cohen.
Neurophotonics, 2015Co-Authors: Yoko Momose-sato, Katsushige SatoAbstract:Investigating the developmental organization of the embryonic nervous system is one of the major challenges in the field of neuroscience. Despite their significance, functional studies on the vertebrate embryonic central nervous system (CNS) have been hampered by the technical limitations associated with conventional electrophysiological methods. The advent of optical techniques using Voltage-Sensitive Dyes, which were developed by Dr. Cohen and his colleagues, has enabled electrical activity in living cells to be monitored noninvasively and also facilitated the simultaneous recording of neural responses from multiple regions. Using optical recording techniques, it is now possible to follow the functional organization of the embryonic CNS and image the spatiotemporal dynamics involved in the formation of this neural network. We herein briefly reviewed optical studies on the embryonic CNS with a special emphasis on methodological considerations and the study of neuronal circuit formation, which demonstrates the utility of fast Voltage-Sensitive Dye imaging as a powerful tool for elucidating the functional organization of the embryonic CNS.
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Using Voltage-Sensitive Dye recording to image the functional development of neuronal circuits in vertebrate embryos.
Developmental neurobiology, 2008Co-Authors: Joel C. Glover, Katsushige Sato, Yoko Momose-satoAbstract:Recent developments in the design of Voltage-Sensitive Dyes and of recording apparatuses for detecting voltage-dependent changes in the optical properties of such Dyes have established Voltage-Sensitive Dye recording as an important technique for assessing the functional development of neuronal circuits in the brain and spinal cord. Here we discuss general technical issues regarding the recording of Voltage-Sensitive Dye signals and describe studies that have utilized this approach to follow the development of sensory and sensorimotor circuits in the embryonic brain stem. Functional imaging through Voltage-Sensitive Dye recording permits a noninvasive analysis of synaptic development and function at submillisecond temporal resolution in widely distributed circuits. These advantages are particularly valuable in assessing sensorimotor circuit development at early stages when neurons are small and synapses are fragile. © 2008 Wiley Periodicals, Inc. Develop Neurobiol, 2008
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Development of vagal afferent projections circumflex to the obex in the embryonic chick brainstem visualized with Voltage-Sensitive Dye recording.
Neuroscience, 2007Co-Authors: Yoko Momose-sato, M. Kinoshita, Katsushige SatoAbstract:Abstract Using Voltage-Sensitive Dye recording, we surveyed neural responses related to the vagus nerve in the embryonic chick brainstem. In our previous studies, we identified four vagus nerve–related response areas in the brainstem. On the stimulated side, they included (1) the nucleus of the tractus solitarius (NTS: the primary sensory nucleus) and (2) the dorsal motor nucleus of the vagus nerve (DMNV), whereas on the contralateral side, they corresponded to (3) the parabrachial nucleus (PBN: the second/higher-ordered nucleus) and (4) the medullary non-NTS region. In the present study, in addition to these areas, we identified another response area circumflex to the obex. The intensity of the optical signal in the response area was much smaller than that in the NTS/DMNV, and the spatio-temporal pattern could be discerned after signal averaging. The conduction rate to the response area was slower than that to the other four areas. Ontogenetically, the response area was distributed on the stimulated side at the 6-day embryonic stage, and it spread into the contralateral side in 7- and 8-day embryonic stages. These distribution patterns were consistent with projection patterns of vagal afferent fibers stained with a fluorescent tracer, suggesting that the response area included a primary sensory nucleus. In comparison with the functional development of the other four response areas, we traced the functional organization of vagus nerve–related nuclei in the embryonic brainstem.
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Depolarization waves in the embryonic CNS triggered by multiple sensory inputs and spontaneous activity: optical imaging with a Voltage-Sensitive Dye.
Neuroscience, 2003Co-Authors: Yoko Momose-sato, Hiraku Mochida, Shinichi Sasaki, Katsushige SatoAbstract:Abstract Previously, we discovered a novel type of depolarization wave in the embryonic chick brain by using a multiple-site optical recording technique with a fast Voltage-Sensitive Dye. This depolarization wave traveled widely over almost all the region of the CNS. This profile has raised the possibility that the depolarization wave plays some global roles in development of the CNS, rather than contributing to a specific neuronal circuit formation. To obtain more information concerning this issue, in the present study, we examined whether the depolarization wave was triggered by various types of peripheral nerve inputs. Stimulation applied to the vagus, glossopharyngeal, cochlear and trigeminal nerves evoked widely spreading depolarization waves with similar spatiotemporal distribution patterns. The developmental sequence of wave expression was parallel to the development of the excitatory postsynaptic potentials in each sensory nucleus. The depolarization wave was accompanied by a Ca 2+ -wave, suggesting that not only electrical synchrony, but also large-scale Ca 2+ -transients may affect developmental processes in the embryonic brain. Furthermore, we found that the depolarization wave also occurred spontaneously. The waveform and distribution patterns of the spontaneous optical signals were similar to those of the cranial nerve-evoked depolarization wave. These results demonstrated that the depolarization wave in the embryonic chick brain is triggered by multiple sources of external and endogenous activity. This profile supports the idea that this depolarization wave may not serve as a simple regulator of specific neuronal circuit formation, but might play more global roles in CNS development.
Peter Andras - One of the best experts on this subject based on the ideXlab platform.
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optical imaging of neurons in the crab stomatogastric ganglion with voltage sensitive Dyes
Journal of Visualized Experiments, 2011Co-Authors: Wolfgang Stein, Carola Stadele, Peter AndrasAbstract:Voltage-Sensitive Dye imaging of neurons is a key methodology for the understanding of how neuronal networks are organised and how the simultaneous activity of participating neurons leads to the emergence of the integral functionality of the network. Here we present the methodology of application of this technique to identified pattern generating neurons in the crab stomatogastric ganglion. We demonstrate the loading of these neurons with the fluorescent Voltage-Sensitive Dye Di-8-ANEPPQ and we show how to image the activity of Dye loaded neurons using the MiCAM02 high speed and high resolution CCD camera imaging system. We demonstrate the analysis of the recorded imaging data using the BVAna imaging software associated with the MiCAM02 imaging system. The simultaneous Voltage-Sensitive Dye imaging of the detailed activity of multiple neurons in the crab stomatogastric ganglion applied together with traditional electrophysiology techniques (intracellular and extracellular recordings) opens radically new opportunities for the understanding of how central pattern generator neural networks work.
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Single-sweep Voltage-Sensitive Dye imaging of interacting identified neurons
Journal of neuroscience methods, 2010Co-Authors: Wolfgang Stein, Carola Stadele, Peter AndrasAbstract:The simultaneous recording of many individual neurons is fundamental to understanding the integral functionality of neural systems. Imaging with Voltage-Sensitive Dyes (VSDs) is a key approach to achieve this goal and a promising technique to supplement electrophysiological recordings. However, the lack of connectivity maps between imaged neurons and the requirement of averaging over repeated trials impede functional interpretations. Here we demonstrate fast, high resolution and single-sweep VSD imaging of identified and synaptically interacting neurons. We show for the first time the optical recording of individual action potentials and mutual inhibitory synaptic input of two key players in the well-characterized pyloric central pattern generator in the crab stomatogastric ganglion (STG). We also demonstrate the presence of individual synaptic potentials from other identified circuit neurons. We argue that imaging of neural networks with identifiable neurons with well-known connectivity, like in the STG, is crucial for the understanding of emergence of network functionality.
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light induced effects of a fluorescent voltage sensitive Dye on neuronal activity in the crab stomatogastric ganglion
Journal of Neuroscience Methods, 2010Co-Authors: Wolfgang Stein, Peter AndrasAbstract:Optical imaging being one of the cutting-edge methods for the investigation of neural activity, it is very important to understand the mechanisms of how Dye molecules work and the range of side effects that they may induce. In particular, it is very important to reveal potential toxic effects and effects impairing the functioning of the investigated neural system. Here, we investigate the effects of illumination in the presence of the commonly used di-4-ANEPPS Voltage-Sensitive Dye on the rhythmic motor pattern generated by the pyloric central pattern generator in the crab stomatogastric nervous system, a model system for motor pattern generation. We report that the Dye allows long recording sessions with little bleaching and no obvious damage to the pyloric rhythm. Yet, exciting illumination induced a temporary and reversible change in the phase relationship of the pyloric motor neurons and a concomitant speed-up of the rhythm. The effect was specific to the excitation wavelength of di-4-ANEPPS and only obtained when the neuropile and cell bodies were illuminated. Thus, di-4-ANEPPS acts as a photo-switch that causes a quick and reversible change in the phase relationship of the motor neurons, but no permanent impairment of neuronal function. It may thus also be used as a means to study the maintenance of phase relationships in rhythmic motor patterns.
K. Murase - One of the best experts on this subject based on the ideXlab platform.
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Synaptic plasticity in the spinal dorsal horn.
Neuroscience research, 2009Co-Authors: H. Ikeda, Takaki Kiritoshi, K. MuraseAbstract:Long-term potentiation (LTP) at synapses in the spinal dorsal horn is thought to be a cellular mechanism for abnormal pain sensitivity. In this article, we review LTP in spinal projection neurons and presynaptic mechanisms of LTP in the spinal dorsal horn revealed by patch-clamp recording and optical imaging with Voltage-Sensitive Dye.
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Optical imaging with retrogradely transported Voltage-Sensitive Dye from projection neurons in marginal layer of the spinal dorsal horn
2003Co-Authors: H. Ikeda, K. Kusudo, K. MuraseAbstract:Projection neurons in the marginal layer of the spinal dorsal horn have been proposed to play an important role for central sensitization. Here, we report a novel method for imaging the neuronal activity from projection neurons to the brainstem in the marginal layer of the spinal dorsal horn by using a retrogradely transported Voltage-Sensitive Dye.
Chun X. Bleau - One of the best experts on this subject based on the ideXlab platform.
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Voltage-Sensitive Dye Imaging of Population Signals in Brain Slices
Cold Spring Harbor protocols, 2015Co-Authors: Bradley J. Baker, Xin Gao, Brian S. Wolff, Lei Jin, Lawrence B. Cohen, Chun X. BleauAbstract:In a bright-field measurement from a vertebrate brain stained by superfusing a solution of the Dye over the surface, each pixel in a camera receives light from a substantial number (thousands) of neurons and neuronal processes (population signals). Because of scattering and out-of-focus light, this will be true even if the pixel size corresponds to a small area of the brain. In this situation, the Voltage-Sensitive Dye signal will be a population average of the change in membrane potential of all of these neurons and processes. Many investigators have published Voltage-Sensitive Dye imaging studies of population activities in brain slices. Their methods, including choice of Dyes, illumination intensity, and imaging device, vary across a large spectrum. Here we present a protocol for visualizing spatiotemporal patterns in rodent neocortex in vitro. Detecting these patterns requires high-sensitivity imaging in single trials, because averaging will obscure the complex dynamics of the spatiotemporal patterns.
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In Vivo Voltage-Sensitive Dye Imaging of Mammalian Cortex Using "Blue" Dyes.
Cold Spring Harbor protocols, 2015Co-Authors: Bradley J. Baker, Xin Gao, Brian S. Wolff, Lei Jin, Lawrence B. Cohen, Chun X. BleauAbstract:Optical recording of membrane potential allows simultaneous measurements to be taken from many different locations in the nervous system. This is important in studies of the nervous system in which simultaneous activity can occur at the regional, cellular, and subcellular levels. New "blue" Dyes, developed by Amiram Grinvald's group, are a great advance for in vivo Voltage-Sensitive Dye imaging of mammalian cortex. The blue Dyes are excited by red light (630 nm) that does not overlap with light absorption of hemoglobin (510-590 nm). This virtually eliminates the heart pulsation artifact.
