The Experts below are selected from a list of 2319 Experts worldwide ranked by ideXlab platform
Ron C Yu - One of the best experts on this subject based on the ideXlab platform.
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pronounced strain specific chemosensory receptor gene expression in the mouse Vomeronasal Organ
BMC Genomics, 2017Co-Authors: Kyle Duyck, Vasha Dutell, Ariel Paulson, Ron C YuAbstract:The chemosensory system plays an important role in orchestrating sexual behaviors in mammals. Pheromones trigger sexually dimorphic behaviors and different mouse strains exhibit differential responses to pheromone stimuli. It has been speculated that differential gene expression in the sensory Organs that detect pheromones may underlie sexually-dimorphic and strain-specific responses to pheromone cues. We have performed transcriptome analyses of the mouse Vomeronasal Organ, a sensory Organ recognizing pheromones and interspecies cues. We find little evidence of sexual dimorphism in gene expression except for Xist, an essential gene for X-linked gene inactivation. Variations in gene expression are found mainly among strains, with genes from immune response and chemosensory receptor classes dominating the list. Differentially expressed genes are concentrated in genomic hotspots enriched in these families of genes. Some chemosensory receptors show exclusive patterns of expression in different strains. We find high levels of single nucleotide polymorphism in chemosensory receptor pseudogenes, some of which lead to functionalized receptors. Moreover, we identify a number of differentially expressed long noncoding RNA species showing strong correlation or anti-correlation with chemoreceptor genes. Our analyses provide little evidence supporting sexually dimorphic gene expression in the Vomeronasal Organ that may underlie dimorphic pheromone responses. In contrast, we find pronounced variations in the expression of immune response related genes, Vomeronasal and G-protein coupled receptor genes among different mouse strains. These findings raised the possibility that diverse strains of mouse perceive pheromone cues differently and behavioral difference among strains in response to pheromone may first arise from differential detection of pheromones. On the other hand, sexually dimorphic responses to pheromones more likely originate from dimorphic neural circuits in the brain than from differential detection. Moreover, noncoding RNA may offer a potential regulatory mechanism controlling the differential expression patterns.
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paradoxical contribution of sk3 and girk channels to the activation of mouse Vomeronasal Organ
Nature Neuroscience, 2012Co-Authors: Kristi L Jensen, Ron C Yu, Chris T Bond, John P AdelmanAbstract:In this study, the authors show that two K+ channels, SK3 and GIRK, are important for the pheromone-induced activation of olfactory neurons in the Vomeronasal Organ (VNO). In addition, they show that K+ ion concentrations are maintained at high levels in the VNO lumen, permitting inward K+ flux through these channels.
Judith M Stern - One of the best experts on this subject based on the ideXlab platform.
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maternal aggression in rats effects of olfactory bulbectomy znso4 induced anosmia and Vomeronasal Organ removal
Hormones and Behavior, 1995Co-Authors: Jane M Kolunie, Judith M SternAbstract:Abstract Previous studies from our laboratory indicate that somatosensory inputs to the snout and ventral trunk, but not visual or auditory stimuli, play critical roles in the elicitation and maintenance of maternal aggression by lactating Norway rats toward a strange male intruder. There are conflicting reports on the influence of olfaction on maternal aggression. We explored the possible roles of central or peripheral anosmia on maternal aggression in Long–Evans rats during early lactation. In Experiment 1, responsiveness to both volatile and non-volatile odors was eliminated by bilateral olfactory bulbectomy (BOB), carried out during mid-gestation. BOB resulted in a reduced likelihood and intensity of maternal aggression on days 1 and 5 of lactation (L1 and L5), but also severe deficiencies in maternal behavior and litter growth and survival. In Experiment 2, anosmia to volatile odors was induced by spraying zinc sulfate intranasally on gestation day 21 and L1. This treatment had little or no effect on maternal aggression on L1 or L2 or on maternal behavior, especially if there was a 1-day recovery between the second treatment and testing. In Experiment 3, responsiveness to non-volatile odors was eliminated by Vomeronasal-Organ removal (VNX) carried out prior to mating. VNX did not disturb maternal behavior on L2–L8 or maternal aggression on L1 or L5. These results and others suggest that the expression of maternal aggression is affected by volatile odors, mediated possibly by accessory chemosensory systems such as the septal Organ, or by neural changes that follow olfactory deafferentation, or both.
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maternal aggression in rats effects of olfactory bulbectomy znso4 induced anosmia and Vomeronasal Organ removal
Hormones and Behavior, 1995Co-Authors: Jane M Kolunie, Judith M SternAbstract:Abstract Previous studies from our laboratory indicate that somatosensory inputs to the snout and ventral trunk, but not visual or auditory stimuli, play critical roles in the elicitation and maintenance of maternal aggression by lactating Norway rats toward a strange male intruder. There are conflicting reports on the influence of olfaction on maternal aggression. We explored the possible roles of central or peripheral anosmia on maternal aggression in Long–Evans rats during early lactation. In Experiment 1, responsiveness to both volatile and non-volatile odors was eliminated by bilateral olfactory bulbectomy (BOB), carried out during mid-gestation. BOB resulted in a reduced likelihood and intensity of maternal aggression on days 1 and 5 of lactation (L1 and L5), but also severe deficiencies in maternal behavior and litter growth and survival. In Experiment 2, anosmia to volatile odors was induced by spraying zinc sulfate intranasally on gestation day 21 and L1. This treatment had little or no effect on maternal aggression on L1 or L2 or on maternal behavior, especially if there was a 1-day recovery between the second treatment and testing. In Experiment 3, responsiveness to non-volatile odors was eliminated by Vomeronasal-Organ removal (VNX) carried out prior to mating. VNX did not disturb maternal behavior on L2–L8 or maternal aggression on L1 or L5. These results and others suggest that the expression of maternal aggression is affected by volatile odors, mediated possibly by accessory chemosensory systems such as the septal Organ, or by neural changes that follow olfactory deafferentation, or both.
Minjung Yoon - One of the best experts on this subject based on the ideXlab platform.
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the expression of androstenone receptor or7d4 in Vomeronasal Organ and olfactory epithelium of horses
Domestic Animal Endocrinology, 2021Co-Authors: Yeonju Choi, Minjung YoonAbstract:Abstract Androstenone is the first mammalian steroidal pheromone to be identified. Pheromones are chemicals that animals use to communicate within a species. Pheromone detections are related to Vomeronasal Organ (VNO) and olfactory epithelium (OE) in mammals. Olfactory Receptor Family 7 Subfamily D Member 4 (OR7D4) is an odorant receptor that responds to androstenone. Several studies indicated that spray with androstenone changes behaviors of the boar and dogs. However, the expression of OR7D4 in VNO and OE was not reported in mammals except human. Thus, the main objectives of this study were to investigate the expression of OR7D4 in VNO and OE of horses. Tissue samples were collected from the VNO and nasal cavity of 6 thoroughbred horses. The presence of OR7D4 gene was investigated with reverse transcription–polymerase chain reaction. The expression of OR7D4 was determined using Western blot and immunofluorescence. As a result, the bands for OR7D4 were observed at approximately 462 bp. The protein band of OR7D4 of VNO and OE was detected at 38 kDa. Immunofluorescence result showed that the cilia and cytoplasm of olfactory receptor cells of VNO and nasal cavity tissues were immunolabeled with OR7D4 antibody. The intensity of OR7D4 protein bands in the ventral region of the ethmoidal concha tissues was not significantly different between mares and geldings. In conclusion, thoroughbred horses are capable of androstenone perception through OR7D4 expressed in the VNO and OE.
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the expression of androstenone receptor or7d4 in Vomeronasal Organ and olfactory epithelium of horses
Domestic Animal Endocrinology, 2020Co-Authors: Yeonju Choi, Minjung YoonAbstract:Abstract Androstenone is the first mammalian steroidal pheromone to be identified. Pheromones are chemicals that animals use to communicate within a species. Pheromone detections are related to Vomeronasal Organ and olfactory epithelium in mammals. OR7D4 is an odorant receptor that responds to androstenone. Several studies indicated that spray with androstenone changes behaviors of the boar and dogs. However, the expression of OR7D4 in Vomeronasal Organ and olfactory epithelium was not reported in mammals except human. Thus, the main objectives of this study was to investigate the expression of OR7D4 in Vomeronasal Organ and olfactory epithelium of horses. Tissue samples were collected from the Vomeronasal Organ and nasal cavity of six Thoroughbred horses. The presence of OR7D4 gene was investigated with reverse transcription polymerase chain reaction. The expression of OR7D4 was determined using western blot and immunofluorescence. As a result, the bands for OR7D4 were observed at approximately 462 bp. The protein band of OR7D4 of Vomeronasal Organ and olfactory epithelium were detected at 38 kDa. Immunofluorescence result showed that the cilia and cytoplasm of olfactory receptor cells of Vomeronasal Organ and nasal cavity tissues were immunolabled with OR7D4 antibody. The intensity of OR7D4 protein bands in the ECV tissues were not significantly different in between mares and geldings. In conclusion, Thoroughbred horses are capable of androstenone perception through OR7D4 expressed in Vomeronasal Organ and olfactory epithelium.
Ariel Paulson - One of the best experts on this subject based on the ideXlab platform.
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pronounced strain specific chemosensory receptor gene expression in the mouse Vomeronasal Organ
BMC Genomics, 2017Co-Authors: Kyle Duyck, Vasha Dutell, Ariel Paulson, Ron C YuAbstract:The chemosensory system plays an important role in orchestrating sexual behaviors in mammals. Pheromones trigger sexually dimorphic behaviors and different mouse strains exhibit differential responses to pheromone stimuli. It has been speculated that differential gene expression in the sensory Organs that detect pheromones may underlie sexually-dimorphic and strain-specific responses to pheromone cues. We have performed transcriptome analyses of the mouse Vomeronasal Organ, a sensory Organ recognizing pheromones and interspecies cues. We find little evidence of sexual dimorphism in gene expression except for Xist, an essential gene for X-linked gene inactivation. Variations in gene expression are found mainly among strains, with genes from immune response and chemosensory receptor classes dominating the list. Differentially expressed genes are concentrated in genomic hotspots enriched in these families of genes. Some chemosensory receptors show exclusive patterns of expression in different strains. We find high levels of single nucleotide polymorphism in chemosensory receptor pseudogenes, some of which lead to functionalized receptors. Moreover, we identify a number of differentially expressed long noncoding RNA species showing strong correlation or anti-correlation with chemoreceptor genes. Our analyses provide little evidence supporting sexually dimorphic gene expression in the Vomeronasal Organ that may underlie dimorphic pheromone responses. In contrast, we find pronounced variations in the expression of immune response related genes, Vomeronasal and G-protein coupled receptor genes among different mouse strains. These findings raised the possibility that diverse strains of mouse perceive pheromone cues differently and behavioral difference among strains in response to pheromone may first arise from differential detection of pheromones. On the other hand, sexually dimorphic responses to pheromones more likely originate from dimorphic neural circuits in the brain than from differential detection. Moreover, noncoding RNA may offer a potential regulatory mechanism controlling the differential expression patterns.
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Pronounced strain-specific chemosensory receptor gene expression in the mouse Vomeronasal Organ
BMC Genomics, 2017Co-Authors: Kyle Duyck, Vasha Dutell, Ariel PaulsonAbstract:Background The chemosensory system plays an important role in orchestrating sexual behaviors in mammals. Pheromones trigger sexually dimorphic behaviors and different mouse strains exhibit differential responses to pheromone stimuli. It has been speculated that differential gene expression in the sensory Organs that detect pheromones may underlie sexually-dimorphic and strain-specific responses to pheromone cues. Results We have performed transcriptome analyses of the mouse Vomeronasal Organ, a sensory Organ recognizing pheromones and interspecies cues. We find little evidence of sexual dimorphism in gene expression except for Xist, an essential gene for X-linked gene inactivation. Variations in gene expression are found mainly among strains, with genes from immune response and chemosensory receptor classes dominating the list. Differentially expressed genes are concentrated in genomic hotspots enriched in these families of genes. Some chemosensory receptors show exclusive patterns of expression in different strains. We find high levels of single nucleotide polymorphism in chemosensory receptor pseudogenes, some of which lead to functionalized receptors. Moreover, we identify a number of differentially expressed long noncoding RNA species showing strong correlation or anti-correlation with chemoreceptor genes. Conclusions Our analyses provide little evidence supporting sexually dimorphic gene expression in the Vomeronasal Organ that may underlie dimorphic pheromone responses. In contrast, we find pronounced variations in the expression of immune response related genes, Vomeronasal and G-protein coupled receptor genes among different mouse strains. These findings raised the possibility that diverse strains of mouse perceive pheromone cues differently and behavioral difference among strains in response to pheromone may first arise from differential detection of pheromones. On the other hand, sexually dimorphic responses to pheromones more likely originate from dimorphic neural circuits in the brain than from differential detection. Moreover, noncoding RNA may offer a potential regulatory mechanism controlling the differential expression patterns.
Vasha Dutell - One of the best experts on this subject based on the ideXlab platform.
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pronounced strain specific chemosensory receptor gene expression in the mouse Vomeronasal Organ
BMC Genomics, 2017Co-Authors: Kyle Duyck, Vasha Dutell, Ariel Paulson, Ron C YuAbstract:The chemosensory system plays an important role in orchestrating sexual behaviors in mammals. Pheromones trigger sexually dimorphic behaviors and different mouse strains exhibit differential responses to pheromone stimuli. It has been speculated that differential gene expression in the sensory Organs that detect pheromones may underlie sexually-dimorphic and strain-specific responses to pheromone cues. We have performed transcriptome analyses of the mouse Vomeronasal Organ, a sensory Organ recognizing pheromones and interspecies cues. We find little evidence of sexual dimorphism in gene expression except for Xist, an essential gene for X-linked gene inactivation. Variations in gene expression are found mainly among strains, with genes from immune response and chemosensory receptor classes dominating the list. Differentially expressed genes are concentrated in genomic hotspots enriched in these families of genes. Some chemosensory receptors show exclusive patterns of expression in different strains. We find high levels of single nucleotide polymorphism in chemosensory receptor pseudogenes, some of which lead to functionalized receptors. Moreover, we identify a number of differentially expressed long noncoding RNA species showing strong correlation or anti-correlation with chemoreceptor genes. Our analyses provide little evidence supporting sexually dimorphic gene expression in the Vomeronasal Organ that may underlie dimorphic pheromone responses. In contrast, we find pronounced variations in the expression of immune response related genes, Vomeronasal and G-protein coupled receptor genes among different mouse strains. These findings raised the possibility that diverse strains of mouse perceive pheromone cues differently and behavioral difference among strains in response to pheromone may first arise from differential detection of pheromones. On the other hand, sexually dimorphic responses to pheromones more likely originate from dimorphic neural circuits in the brain than from differential detection. Moreover, noncoding RNA may offer a potential regulatory mechanism controlling the differential expression patterns.
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Pronounced strain-specific chemosensory receptor gene expression in the mouse Vomeronasal Organ
BMC Genomics, 2017Co-Authors: Kyle Duyck, Vasha Dutell, Ariel PaulsonAbstract:Background The chemosensory system plays an important role in orchestrating sexual behaviors in mammals. Pheromones trigger sexually dimorphic behaviors and different mouse strains exhibit differential responses to pheromone stimuli. It has been speculated that differential gene expression in the sensory Organs that detect pheromones may underlie sexually-dimorphic and strain-specific responses to pheromone cues. Results We have performed transcriptome analyses of the mouse Vomeronasal Organ, a sensory Organ recognizing pheromones and interspecies cues. We find little evidence of sexual dimorphism in gene expression except for Xist, an essential gene for X-linked gene inactivation. Variations in gene expression are found mainly among strains, with genes from immune response and chemosensory receptor classes dominating the list. Differentially expressed genes are concentrated in genomic hotspots enriched in these families of genes. Some chemosensory receptors show exclusive patterns of expression in different strains. We find high levels of single nucleotide polymorphism in chemosensory receptor pseudogenes, some of which lead to functionalized receptors. Moreover, we identify a number of differentially expressed long noncoding RNA species showing strong correlation or anti-correlation with chemoreceptor genes. Conclusions Our analyses provide little evidence supporting sexually dimorphic gene expression in the Vomeronasal Organ that may underlie dimorphic pheromone responses. In contrast, we find pronounced variations in the expression of immune response related genes, Vomeronasal and G-protein coupled receptor genes among different mouse strains. These findings raised the possibility that diverse strains of mouse perceive pheromone cues differently and behavioral difference among strains in response to pheromone may first arise from differential detection of pheromones. On the other hand, sexually dimorphic responses to pheromones more likely originate from dimorphic neural circuits in the brain than from differential detection. Moreover, noncoding RNA may offer a potential regulatory mechanism controlling the differential expression patterns.
