The Experts below are selected from a list of 161100 Experts worldwide ranked by ideXlab platform
Ren-xi Zhuo - One of the best experts on this subject based on the ideXlab platform.
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water soluble Polymer protected lipofectamine 2000 dna complexes for solid phase transfection
Macromolecular Bioscience, 2009Co-Authors: Qiao Zhang, Si-xue Cheng, Xian-zheng Zhang, Ren-xi ZhuoAbstract:A fast degrading cholic acid-functionalized star poly(DL-lactide) has been used to fabricate Polymer films to support Lipofectamine 2000/DNA complexes for mediating solid-phase transfection. To improve the gene expression activity, a Water-Soluble Polymer, poly-alpha,beta-[N-(2-hydroxyethyl)-L-aspartamide (PHEA), was added to protect the complexes. The in vitro gene transfection in 293T cells, HeLa cells, and 3T3 cells showed that the gene expressions could be effectively mediated by the deposited Lipofectamine 2000/DNA complexes encapsulated in Polymer films. The degradation of the Polymer films that occurred during gene transfection did not show any unfavorable effects on the gene expression.
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Water soluble Polymer protected lipofectamine 2000/DNA complexes for solid-phase transfection.
Macromolecular bioscience, 2009Co-Authors: Qiao Zhang, Si-xue Cheng, Xian-zheng Zhang, Ren-xi ZhuoAbstract:A fast degrading cholic acid-functionalized star poly(DL-lactide) has been used to fabricate Polymer films to support Lipofectamine 2000/DNA complexes for mediating solid-phase transfection. To improve the gene expression activity, a Water-Soluble Polymer, poly-alpha,beta-[N-(2-hydroxyethyl)-L-aspartamide (PHEA), was added to protect the complexes. The in vitro gene transfection in 293T cells, HeLa cells, and 3T3 cells showed that the gene expressions could be effectively mediated by the deposited Lipofectamine 2000/DNA complexes encapsulated in Polymer films. The degradation of the Polymer films that occurred during gene transfection did not show any unfavorable effects on the gene expression.
Qiao Zhang - One of the best experts on this subject based on the ideXlab platform.
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water soluble Polymer protected lipofectamine 2000 dna complexes for solid phase transfection
Macromolecular Bioscience, 2009Co-Authors: Qiao Zhang, Si-xue Cheng, Xian-zheng Zhang, Ren-xi ZhuoAbstract:A fast degrading cholic acid-functionalized star poly(DL-lactide) has been used to fabricate Polymer films to support Lipofectamine 2000/DNA complexes for mediating solid-phase transfection. To improve the gene expression activity, a Water-Soluble Polymer, poly-alpha,beta-[N-(2-hydroxyethyl)-L-aspartamide (PHEA), was added to protect the complexes. The in vitro gene transfection in 293T cells, HeLa cells, and 3T3 cells showed that the gene expressions could be effectively mediated by the deposited Lipofectamine 2000/DNA complexes encapsulated in Polymer films. The degradation of the Polymer films that occurred during gene transfection did not show any unfavorable effects on the gene expression.
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Water soluble Polymer protected lipofectamine 2000/DNA complexes for solid-phase transfection.
Macromolecular bioscience, 2009Co-Authors: Qiao Zhang, Si-xue Cheng, Xian-zheng Zhang, Ren-xi ZhuoAbstract:A fast degrading cholic acid-functionalized star poly(DL-lactide) has been used to fabricate Polymer films to support Lipofectamine 2000/DNA complexes for mediating solid-phase transfection. To improve the gene expression activity, a Water-Soluble Polymer, poly-alpha,beta-[N-(2-hydroxyethyl)-L-aspartamide (PHEA), was added to protect the complexes. The in vitro gene transfection in 293T cells, HeLa cells, and 3T3 cells showed that the gene expressions could be effectively mediated by the deposited Lipofectamine 2000/DNA complexes encapsulated in Polymer films. The degradation of the Polymer films that occurred during gene transfection did not show any unfavorable effects on the gene expression.
Si-xue Cheng - One of the best experts on this subject based on the ideXlab platform.
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water soluble Polymer protected lipofectamine 2000 dna complexes for solid phase transfection
Macromolecular Bioscience, 2009Co-Authors: Qiao Zhang, Si-xue Cheng, Xian-zheng Zhang, Ren-xi ZhuoAbstract:A fast degrading cholic acid-functionalized star poly(DL-lactide) has been used to fabricate Polymer films to support Lipofectamine 2000/DNA complexes for mediating solid-phase transfection. To improve the gene expression activity, a Water-Soluble Polymer, poly-alpha,beta-[N-(2-hydroxyethyl)-L-aspartamide (PHEA), was added to protect the complexes. The in vitro gene transfection in 293T cells, HeLa cells, and 3T3 cells showed that the gene expressions could be effectively mediated by the deposited Lipofectamine 2000/DNA complexes encapsulated in Polymer films. The degradation of the Polymer films that occurred during gene transfection did not show any unfavorable effects on the gene expression.
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Water soluble Polymer protected lipofectamine 2000/DNA complexes for solid-phase transfection.
Macromolecular bioscience, 2009Co-Authors: Qiao Zhang, Si-xue Cheng, Xian-zheng Zhang, Ren-xi ZhuoAbstract:A fast degrading cholic acid-functionalized star poly(DL-lactide) has been used to fabricate Polymer films to support Lipofectamine 2000/DNA complexes for mediating solid-phase transfection. To improve the gene expression activity, a Water-Soluble Polymer, poly-alpha,beta-[N-(2-hydroxyethyl)-L-aspartamide (PHEA), was added to protect the complexes. The in vitro gene transfection in 293T cells, HeLa cells, and 3T3 cells showed that the gene expressions could be effectively mediated by the deposited Lipofectamine 2000/DNA complexes encapsulated in Polymer films. The degradation of the Polymer films that occurred during gene transfection did not show any unfavorable effects on the gene expression.
Xian-zheng Zhang - One of the best experts on this subject based on the ideXlab platform.
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water soluble Polymer protected lipofectamine 2000 dna complexes for solid phase transfection
Macromolecular Bioscience, 2009Co-Authors: Qiao Zhang, Si-xue Cheng, Xian-zheng Zhang, Ren-xi ZhuoAbstract:A fast degrading cholic acid-functionalized star poly(DL-lactide) has been used to fabricate Polymer films to support Lipofectamine 2000/DNA complexes for mediating solid-phase transfection. To improve the gene expression activity, a Water-Soluble Polymer, poly-alpha,beta-[N-(2-hydroxyethyl)-L-aspartamide (PHEA), was added to protect the complexes. The in vitro gene transfection in 293T cells, HeLa cells, and 3T3 cells showed that the gene expressions could be effectively mediated by the deposited Lipofectamine 2000/DNA complexes encapsulated in Polymer films. The degradation of the Polymer films that occurred during gene transfection did not show any unfavorable effects on the gene expression.
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Water soluble Polymer protected lipofectamine 2000/DNA complexes for solid-phase transfection.
Macromolecular bioscience, 2009Co-Authors: Qiao Zhang, Si-xue Cheng, Xian-zheng Zhang, Ren-xi ZhuoAbstract:A fast degrading cholic acid-functionalized star poly(DL-lactide) has been used to fabricate Polymer films to support Lipofectamine 2000/DNA complexes for mediating solid-phase transfection. To improve the gene expression activity, a Water-Soluble Polymer, poly-alpha,beta-[N-(2-hydroxyethyl)-L-aspartamide (PHEA), was added to protect the complexes. The in vitro gene transfection in 293T cells, HeLa cells, and 3T3 cells showed that the gene expressions could be effectively mediated by the deposited Lipofectamine 2000/DNA complexes encapsulated in Polymer films. The degradation of the Polymer films that occurred during gene transfection did not show any unfavorable effects on the gene expression.
Bernabé L. Rivas - One of the best experts on this subject based on the ideXlab platform.
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Water-Soluble and Insoluble Polymers, Nanoparticles, Nanocomposites and Hybrids With Ability to Remove Hazardous Inorganic Pollutants in Water.
Frontiers in chemistry, 2018Co-Authors: Bernabé L. Rivas, Bruno F. Urbano, Julio SánchezAbstract:The Polymeric materials have presented a great development in adsorption processes for the treatment of polluted waters. The aim of the current review is to present the recent developments in this field of study by examining research of systems like functional Water-Soluble Polymers and Water-Soluble Polymer-metal complexes coupled to ultrafiltration membranes for decontamination processes in liquid-liquid phase. Noticing that a Water-Soluble Polymer can be turned into insoluble compounds by setting a crosslinking point, connecting the Polymer chains leading to Polymer resins suitable for solid-liquid extraction processes. Moreover, these crosslinked Polymers can be used to develop more complex systems such as (nano)composite and hybrid adsorbents, combining the Polymers with inorganic moieties such as metal oxides. This combination results in novel materials that overcome some drawbacks of each separated components and enhance the sorption performance. In addition, new trends in hybrid methods combining of Water-Soluble Polymers, membranes, and electrocatalysis/photocatalysis to remove inorganic pollutants have been discussed in this review.
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Arsenic extraction from aqueous solution: electrochemical oxidation combined with ultrafiltration membranes and Water-Soluble Polymers.
Chemical Engineering Journal, 2010Co-Authors: Julio Sánchez, Bernabé L. RivasAbstract:Abstract This study shows that the combination of the liquid-phase Polymer based retention technique with the electrocatalytic oxidation of arsenic(III) to arsenic(V) is an effective process to remove hazardous As(III) species from aqueous solution. The solution of arsenite and Water-Soluble Polymers at 20:1 Polymer:As(III) molar ratio (As(III) 7.5 × 10−4 M, Water-Soluble Polymer 15 × 10−3 M) was submitted to electrocatalytic oxidation using (carbon/Polymer/Pt) nanocomposite modified electrode at +0.7 V vs. Ag/AgCl and Water-Soluble Polymer as a support electrolyte and extracting agent. After the exhaustive oxidation of As(III) to As(V), the arsenic was placed in the ultrafitration cell and the retention capacity of the Water-Soluble Polymer was studied at different pH. At basic pH, the arsenic retention reached was between 70% and 100%, and the maximum for Water-Soluble Polymer was obtained with chloride as a counter ion: 100% for P(ClDDA), 94% for P(ClAPTA) and 70% for P(SAETA) at Z = 10. The enrichment method shows the maximum retention capacity for arsenate anions in aqueous solutions at pH 9. The type of anion exchanger was an important factor in the maximum retention capacity of arsenate.
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Interactions of 2,3,5-triphenyl-2H-tetrazolium chloride with poly(sodium 4-styrenesulfonate) studied by diafiltration and UV¿vis spectroscopy
Journal of Membrane Science, 2004Co-Authors: Ignacio Moreno-villoslada, Víctor Miranda, Robin Gutiérrez, Susan Hess, Carla Muñoz, Bernabé L. RivasAbstract:Abstract The interaction of the Water-Soluble Polymer poly(sodium 4-styrenesulfonate) with 2,3,5-triphenyl-2 H -tetrazolium chloride is studied. Diafiltration experiments at pH 4, 7.5, and 10, and in the presence of NaNO 3 at different concentrations show weak interactions of the low molecular weight molecule and the Polymer with apparent dissociation constants ranging between 0.6 and 6, and strong interactions, detectable in the absence of NaNO 3 , with apparent dissociation constants ranging between 0.04 and 0.2, if the effect of the interactions of the Water-Soluble Polymer with the diafiltration cell components is neglected. Apart from hydrophobic forces this is attributed to both long-range and short-range electrostatic forces. The short-range interaction is reflected in UV–vis spectroscopic studies by a decrease on the intensity of the maximum at 248 nm.
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Water-Soluble Polymer–metal ion interactions
Progress in Polymer Science, 2003Co-Authors: Bernabé L. Rivas, Eduardo Pereira, Ignacio Moreno-villosladaAbstract:Abstract The interactions between metal ions and Water-Soluble Polymers (WSP) have gained great interest due to their intrinsic properties as well as their potential applications, such as superconducting materials, ultra-high strength materials, liquid crystals, and biocompatible Polymers. This review attempts to provide a general coverage of various scientific aspects of the WSP–metal ion interactions in aqueous solution and their applications. It includes fundamental aspects on synthesis of Water-Soluble Polymers, as well as different activities and properties. A particular emphasis is given to the study the WSP–metal ion interactions, under different experimental procedures, through the liquid-phase Polymer based retention (LPR) technique, which combines the use of WSP and membrane ultrafiltration. Theoretical work on this WSP–metal ion interaction has been elaborated to explain the counterion binding to polyelectrolytes, and the Polymer–metal ion complex formation.
