The Experts below are selected from a list of 36 Experts worldwide ranked by ideXlab platform
Chen Zheng - One of the best experts on this subject based on the ideXlab platform.
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Wnt2 Protein plays a role in the progression of pancreatic cancer promoted by pancreatic stellate cells
Medical Oncology, 2015Co-Authors: Yong Xu, Hua Li, Chongbiao Huang, Tiansuo Zhao, Huan Zhang, Chen ZhengAbstract:This study aimed to investigate the expression of Wnt2 Protein in pancreatic cancer tissues and pancreatic stellate cells (PSCs), and determine its effect on the biological functions of pancreatic cancer cells. Immunohistochemistry was used to study the expression pattern of Wnt2 in pancreatic cancer tissues. The relationship between Wnt2 Protein expression level and patient prognosis was analyzed. PSCs were isolated and cultured. The expression of Wnt2 in activated PSCs was investigated using Western blot and immunofluorescence. We also analyzed the effect of Wnt2 recombinant Protein and stellate cell culture supernatant on the Wnt/β-catenin signaling pathway, as well as the effect of Wnt2 recombinant Protein on the biological functions of pancreatic cancer cells. The expression of Wnt2 in interstitial cells of pancreatic cancer was correlated with the prognosis of pancreatic cancer. Wnt2 Protein was expressed in activated PSCs. Both stellate cell culture supernatant and Wnt2 recombinant Protein could activate the classic Wnt/β-catenin signaling pathway. Wnt2 Protein enhanced the migration, invasion, and metastasis of pancreatic cancer cells. These results suggested that Wnt2 Protein secreted by PSCs promoted the progression of pancreatic cancer by activating the classic Wnt/β-catenin signaling pathway.
Yong Xu - One of the best experts on this subject based on the ideXlab platform.
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Wnt2 Protein plays a role in the progression of pancreatic cancer promoted by pancreatic stellate cells
Medical Oncology, 2015Co-Authors: Yong Xu, Hua Li, Chongbiao Huang, Tiansuo Zhao, Huan Zhang, Chen ZhengAbstract:This study aimed to investigate the expression of Wnt2 Protein in pancreatic cancer tissues and pancreatic stellate cells (PSCs), and determine its effect on the biological functions of pancreatic cancer cells. Immunohistochemistry was used to study the expression pattern of Wnt2 in pancreatic cancer tissues. The relationship between Wnt2 Protein expression level and patient prognosis was analyzed. PSCs were isolated and cultured. The expression of Wnt2 in activated PSCs was investigated using Western blot and immunofluorescence. We also analyzed the effect of Wnt2 recombinant Protein and stellate cell culture supernatant on the Wnt/β-catenin signaling pathway, as well as the effect of Wnt2 recombinant Protein on the biological functions of pancreatic cancer cells. The expression of Wnt2 in interstitial cells of pancreatic cancer was correlated with the prognosis of pancreatic cancer. Wnt2 Protein was expressed in activated PSCs. Both stellate cell culture supernatant and Wnt2 recombinant Protein could activate the classic Wnt/β-catenin signaling pathway. Wnt2 Protein enhanced the migration, invasion, and metastasis of pancreatic cancer cells. These results suggested that Wnt2 Protein secreted by PSCs promoted the progression of pancreatic cancer by activating the classic Wnt/β-catenin signaling pathway.
Sarah J. George - One of the best experts on this subject based on the ideXlab platform.
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Wnt2 and WISP-1/CCN4 Induce Intimal Thickening via Promotion of Smooth Muscle Cell Migration
Arteriosclerosis Thrombosis and Vascular Biology, 2016Co-Authors: Helen Williams, Carina Mill, Jason L. Johnson, Bethan Alice Monk, Sarah Hulin-curtis, Sarah J. GeorgeAbstract:Objective—Increased vascular smooth muscle cell (VSMC) migration leads to intimal thickening which acts as a soil for atherosclersosis, as well as causing coronary artery restenosis after stenting and vein graft failure. Investigating factors involved in VSMC migration may enable us to reduce intimal thickening and improve patient outcomes. In this study, we determined whether Wnt Proteins regulate VSMC migration and thereby intimal thickening. Approach and Results—Wnt2 mRNA and Protein expression were specifically increased in migrating mouse aortic VSMCs. Moreover, VSMC migration was induced by recombinant Wnt2 in vitro. Addition of recombinant Wnt2 Protein increased Wnt1-inducible signaling pathway Protein-1 (WISP-1) mRNA by ≈1.7-fold, via β-catenin/T-cell factor signaling, whereas silencing RNA knockdown of Wnt-2 reduced WISP-1 mRNA by ≈65%. Treatment with rWISP-1 significantly increased VSMC migration by ≈1.5-fold, whereas WISP-1 silencing RNA knockdown reduced migration by ≈40%. Wnt2 and WISP-1 effe...
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WNT INDUCIBLE SOLUBLE Protein 1 (WISP-1) PROMOTES VSMC MIGRATION AND INTIMAL THICKENING
Atherosclerosis, 2014Co-Authors: Helen Williams, Carina Mill, S. Curtis, Jason L. Johnson, Sarah J. GeorgeAbstract:Vascular smooth muscle cell (VSMC) migration promotes coronary artery restenosis and vein graft failure. Therefore investigating factors involved in VSMC migration may enable us to improve treatments and therefore patient outcomes. We have previously shown that augmented Wnt2 expression occurred in and increased VSMC migration in vitro. This study aimed to further elucidate the mechanism of action of Wnt2. Addition of recombinant Wnt2 Protein increased WISP-1 mRNA by 1.7±0.1 fold (p
Hua Li - One of the best experts on this subject based on the ideXlab platform.
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Wnt2 Protein plays a role in the progression of pancreatic cancer promoted by pancreatic stellate cells
Medical Oncology, 2015Co-Authors: Yong Xu, Hua Li, Chongbiao Huang, Tiansuo Zhao, Huan Zhang, Chen ZhengAbstract:This study aimed to investigate the expression of Wnt2 Protein in pancreatic cancer tissues and pancreatic stellate cells (PSCs), and determine its effect on the biological functions of pancreatic cancer cells. Immunohistochemistry was used to study the expression pattern of Wnt2 in pancreatic cancer tissues. The relationship between Wnt2 Protein expression level and patient prognosis was analyzed. PSCs were isolated and cultured. The expression of Wnt2 in activated PSCs was investigated using Western blot and immunofluorescence. We also analyzed the effect of Wnt2 recombinant Protein and stellate cell culture supernatant on the Wnt/β-catenin signaling pathway, as well as the effect of Wnt2 recombinant Protein on the biological functions of pancreatic cancer cells. The expression of Wnt2 in interstitial cells of pancreatic cancer was correlated with the prognosis of pancreatic cancer. Wnt2 Protein was expressed in activated PSCs. Both stellate cell culture supernatant and Wnt2 recombinant Protein could activate the classic Wnt/β-catenin signaling pathway. Wnt2 Protein enhanced the migration, invasion, and metastasis of pancreatic cancer cells. These results suggested that Wnt2 Protein secreted by PSCs promoted the progression of pancreatic cancer by activating the classic Wnt/β-catenin signaling pathway.
Chongbiao Huang - One of the best experts on this subject based on the ideXlab platform.
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Wnt2 Protein plays a role in the progression of pancreatic cancer promoted by pancreatic stellate cells
Medical Oncology, 2015Co-Authors: Yong Xu, Hua Li, Chongbiao Huang, Tiansuo Zhao, Huan Zhang, Chen ZhengAbstract:This study aimed to investigate the expression of Wnt2 Protein in pancreatic cancer tissues and pancreatic stellate cells (PSCs), and determine its effect on the biological functions of pancreatic cancer cells. Immunohistochemistry was used to study the expression pattern of Wnt2 in pancreatic cancer tissues. The relationship between Wnt2 Protein expression level and patient prognosis was analyzed. PSCs were isolated and cultured. The expression of Wnt2 in activated PSCs was investigated using Western blot and immunofluorescence. We also analyzed the effect of Wnt2 recombinant Protein and stellate cell culture supernatant on the Wnt/β-catenin signaling pathway, as well as the effect of Wnt2 recombinant Protein on the biological functions of pancreatic cancer cells. The expression of Wnt2 in interstitial cells of pancreatic cancer was correlated with the prognosis of pancreatic cancer. Wnt2 Protein was expressed in activated PSCs. Both stellate cell culture supernatant and Wnt2 recombinant Protein could activate the classic Wnt/β-catenin signaling pathway. Wnt2 Protein enhanced the migration, invasion, and metastasis of pancreatic cancer cells. These results suggested that Wnt2 Protein secreted by PSCs promoted the progression of pancreatic cancer by activating the classic Wnt/β-catenin signaling pathway.
