The Experts below are selected from a list of 36 Experts worldwide ranked by ideXlab platform
Antoine M Saab - One of the best experts on this subject based on the ideXlab platform.
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antiproliferative antimicrobial and antioxidant properties of cedrus libani and pinus pinea Wood Oils and juniperus excelsa berry oil
Plant Biosystems, 2020Co-Authors: Alessandro Venditti, Antoine M Saab, Filippo Maggi, Massimo Bramucci, Luana Quassinti, Dezemona Petrelli, Luca Agostino Vitali, Giulio Lupidi, A El G Samrani, Monica BorgattiAbstract:In this paper we report the analysis of essential Oils from the Woods of C. libani and P. pinea and from the berries of J. excelsa. Several differences were observed with respect to previous studie...
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in vitro evaluation of the anti proliferative activities of the Wood essential Oils of three cedrus species against k562 human chronic myelogenous leukaemia cells
Natural Product Research, 2012Co-Authors: Antoine M Saab, Ilaria Lampronti, Monica Borgatti, Alessia Finotti, Faouzi Harb, Samir Safi, Roberto GambariAbstract:There are four kinds of Cedar: Cedrus libani naturally occurring in Lebanon, Syria and Turkey, Cedrus atlantica in Morocco and Algeria, Cedrus brevefolia in Cyprus Island and Cedrus deodara which is distributed in Himalayan Mountains. Wood essential Oils obtained from C. libani, C. atlantica and C. deodara were tested for the inhibition of K562 cell proliferation and for the induction of erythroid differentiation. The Wood essential Oils of C. libani, C. atlantica and C. deodara inhibited the proliferation of the K562 cell line exhibiting IC50 values 23.38 ± 1.7, 59.37 ± 2.6 and 37.09 ± 1.4 µg mL−1, respectively. Meanwhile, C. libani Wood Oils induced a percentage of erythroid differentiation of 15 ± 2% at concentration 5 µg mL−1. Cedrus deodara Wood oil indicated a percentage of erythroid differentiation of 20 ± 2% at concentration 25 µg mL−1 and C. atlantica Wood Oils showed a percentage of erythroid differentiation of 12 ± 1.8% at concentration 10 µg mL−1.
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comparative chemical composition antioxidant and hypoglycaemic activities of juniperus oxycedrus ssp oxycedrus l berry and Wood Oils from lebanon
Food Chemistry, 2007Co-Authors: Monica Rosa Loizzo, Antoine M Saab, Rosa Tundis, Filomena Conforti, Giancarlo A Statti, Francesco MenichiniAbstract:Abstract Juniper ( Juniperus oxycedrus ) is used in European cuisine for its distinguishing flavour. J. oxycedrus ssp. oxycedrus berry and Wood essential Oils were tentatively identified by GC and GC/MS. Fifty compounds were identified in the berry oil and 23 compounds were identified in the Wood oil. The J. oxycedrus ssp. oxycedrus berry oil was characterised by high contents of α-pinene (27.4%) and β-myrcene (18.9%). Other important compounds were α-phellandrene (7.1%), limonene (6.7%), epi -bicyclosesquiphellandrene (2.3%) and δ-cadinene (2.2%) while, in the Wood oil, δ-cadinene (14.5%) is a major main component, together with cis -thujopsene (9.2%) and α-muurolene (4.9%). In vitro evaluation of antioxidant activity by the DPPH method showed a significant activity for both Oils with IC 50 values of 1.45 μl/ml for Wood and 7.42 μl/ml for berries. Hypoglycaemic activity was investigated through the inhibition of α-amylase. The results revealed that oil obtained by hydrodistillation from J. oxycedrus ssp. oxycedrus Wood exhibits an interesting activity with IC 50 of 3.49 μl/ml.
Yi Ling - One of the best experts on this subject based on the ideXlab platform.
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characterization of a sesquiterpene cyclase from the glandular trichomes of leucosceptrum canum for sole production of cedrol in escherichia coli and nicotiana benthamiana
Phytochemistry, 2019Co-Authors: Fei Luo, Yi Ling, Ting Tang, Yanchun Liu, Yan LiuAbstract:Cedrol is an extremely versatile sesquiterpene alcohol that was approved by the Food and Drug Administration of the United States as a flavoring agent or adjuvant and has been commonly used as a flavoring ingredient in cosmetics, foods and medicine. Furthermore, cedrol possesses a wide range of pharmacological properties including sedative, anti-inflammatory and cytotoxic activities. Commercial production of cedrol relies on fractional distillation of cedar Wood Oils, followed by recrystallization, and little has been reported about its biosynthesis and aspects of synthetic biology. Here, we report the cloning and functional characterization of a cedrol synthase gene (Lc-CedS) from the transcriptome of the glandular trichomes of a Woody Lamiaceae plant Leucosceptrum canum. The recombinant Lc-CedS protein catalyzed the in vitro conversion of farnesyl diphosphate into the single product cedrol, suggesting that Lc-CedS is a high-fidelity terpene synthase. Co-expression of Lc-CedS, a farnesyl diphosphate synthase gene and seven genes of the mevalonate (MVA) pathway responsible for converting acetyl-CoA into farnesyl diphosphate in Escherichia coli afforded 363 μg/L cedrol as the sole product under shaking flask conditions. Transient expression of Lc-CedS in Nicotiana benthamiana also resulted in a single product cedrol with a production level of 3.6 μg/g fresh weight. The sole production of cedrol by introducing of Lc-CedS in engineered E. coli and N. benthamiana suggests now alternative production systems using synthetic biology approaches that would better address sufficient supply of cedrol.
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characterization of a sesquiterpene cyclase from the glandular trichomes of leucosceptrum canum for sole production of cedrol in escherichia coli and nicotiana benthamiana
Phytochemistry, 2019Co-Authors: Yi Ling, Ting Tang, Desen Li, Shenghong LiAbstract:Cedrol is an extremely versatile sesquiterpene alcohol that was approved by the Food and Drug Administration of the United States as a flavoring agent or adjuvant and has been commonly used as a flavoring ingredient in cosmetics, foods and medicine. Furthermore, cedrol possesses a wide range of pharmacological properties including sedative, anti-inflammatory and cytotoxic activities. Commercial production of cedrol relies on fractional distillation of cedar Wood Oils, followed by recrystallization, and little has been reported about its biosynthesis and aspects of synthetic biology. Here, we report the cloning and functional characterization of a cedrol synthase gene (Lc-CedS) from the transcriptome of the glandular trichomes of a Woody Lamiaceae plant Leucosceptrum canum. The recombinant Lc-CedS protein catalyzed the in vitro conversion of farnesyl diphosphate into the single product cedrol, suggesting that Lc-CedS is a high-fidelity terpene synthase. Co-expression of Lc-CedS, a farnesyl diphosphate synthase gene and seven genes of the mevalonate (MVA) pathway responsible for converting acetyl-CoA into farnesyl diphosphate in Escherichia coli afforded 363 μg/L cedrol as the sole product under shaking flask conditions. Transient expression of Lc-CedS in Nicotiana benthamiana also resulted in a single product cedrol with a production level of 3.6 μg/g fresh weight. The sole production of cedrol by introducing of Lc-CedS in engineered E. coli and N. benthamiana suggests now alternative production systems using synthetic biology approaches that would better address sufficient supply of cedrol.
Francesco Menichini - One of the best experts on this subject based on the ideXlab platform.
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comparative chemical composition antioxidant and hypoglycaemic activities of juniperus oxycedrus ssp oxycedrus l berry and Wood Oils from lebanon
Food Chemistry, 2007Co-Authors: Monica Rosa Loizzo, Antoine M Saab, Rosa Tundis, Filomena Conforti, Giancarlo A Statti, Francesco MenichiniAbstract:Abstract Juniper ( Juniperus oxycedrus ) is used in European cuisine for its distinguishing flavour. J. oxycedrus ssp. oxycedrus berry and Wood essential Oils were tentatively identified by GC and GC/MS. Fifty compounds were identified in the berry oil and 23 compounds were identified in the Wood oil. The J. oxycedrus ssp. oxycedrus berry oil was characterised by high contents of α-pinene (27.4%) and β-myrcene (18.9%). Other important compounds were α-phellandrene (7.1%), limonene (6.7%), epi -bicyclosesquiphellandrene (2.3%) and δ-cadinene (2.2%) while, in the Wood oil, δ-cadinene (14.5%) is a major main component, together with cis -thujopsene (9.2%) and α-muurolene (4.9%). In vitro evaluation of antioxidant activity by the DPPH method showed a significant activity for both Oils with IC 50 values of 1.45 μl/ml for Wood and 7.42 μl/ml for berries. Hypoglycaemic activity was investigated through the inhibition of α-amylase. The results revealed that oil obtained by hydrodistillation from J. oxycedrus ssp. oxycedrus Wood exhibits an interesting activity with IC 50 of 3.49 μl/ml.
Shenghong Li - One of the best experts on this subject based on the ideXlab platform.
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characterization of a sesquiterpene cyclase from the glandular trichomes of leucosceptrum canum for sole production of cedrol in escherichia coli and nicotiana benthamiana
Phytochemistry, 2019Co-Authors: Yi Ling, Ting Tang, Desen Li, Shenghong LiAbstract:Cedrol is an extremely versatile sesquiterpene alcohol that was approved by the Food and Drug Administration of the United States as a flavoring agent or adjuvant and has been commonly used as a flavoring ingredient in cosmetics, foods and medicine. Furthermore, cedrol possesses a wide range of pharmacological properties including sedative, anti-inflammatory and cytotoxic activities. Commercial production of cedrol relies on fractional distillation of cedar Wood Oils, followed by recrystallization, and little has been reported about its biosynthesis and aspects of synthetic biology. Here, we report the cloning and functional characterization of a cedrol synthase gene (Lc-CedS) from the transcriptome of the glandular trichomes of a Woody Lamiaceae plant Leucosceptrum canum. The recombinant Lc-CedS protein catalyzed the in vitro conversion of farnesyl diphosphate into the single product cedrol, suggesting that Lc-CedS is a high-fidelity terpene synthase. Co-expression of Lc-CedS, a farnesyl diphosphate synthase gene and seven genes of the mevalonate (MVA) pathway responsible for converting acetyl-CoA into farnesyl diphosphate in Escherichia coli afforded 363 μg/L cedrol as the sole product under shaking flask conditions. Transient expression of Lc-CedS in Nicotiana benthamiana also resulted in a single product cedrol with a production level of 3.6 μg/g fresh weight. The sole production of cedrol by introducing of Lc-CedS in engineered E. coli and N. benthamiana suggests now alternative production systems using synthetic biology approaches that would better address sufficient supply of cedrol.
Yan Liu - One of the best experts on this subject based on the ideXlab platform.
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characterization of a sesquiterpene cyclase from the glandular trichomes of leucosceptrum canum for sole production of cedrol in escherichia coli and nicotiana benthamiana
Phytochemistry, 2019Co-Authors: Fei Luo, Yi Ling, Ting Tang, Yanchun Liu, Yan LiuAbstract:Cedrol is an extremely versatile sesquiterpene alcohol that was approved by the Food and Drug Administration of the United States as a flavoring agent or adjuvant and has been commonly used as a flavoring ingredient in cosmetics, foods and medicine. Furthermore, cedrol possesses a wide range of pharmacological properties including sedative, anti-inflammatory and cytotoxic activities. Commercial production of cedrol relies on fractional distillation of cedar Wood Oils, followed by recrystallization, and little has been reported about its biosynthesis and aspects of synthetic biology. Here, we report the cloning and functional characterization of a cedrol synthase gene (Lc-CedS) from the transcriptome of the glandular trichomes of a Woody Lamiaceae plant Leucosceptrum canum. The recombinant Lc-CedS protein catalyzed the in vitro conversion of farnesyl diphosphate into the single product cedrol, suggesting that Lc-CedS is a high-fidelity terpene synthase. Co-expression of Lc-CedS, a farnesyl diphosphate synthase gene and seven genes of the mevalonate (MVA) pathway responsible for converting acetyl-CoA into farnesyl diphosphate in Escherichia coli afforded 363 μg/L cedrol as the sole product under shaking flask conditions. Transient expression of Lc-CedS in Nicotiana benthamiana also resulted in a single product cedrol with a production level of 3.6 μg/g fresh weight. The sole production of cedrol by introducing of Lc-CedS in engineered E. coli and N. benthamiana suggests now alternative production systems using synthetic biology approaches that would better address sufficient supply of cedrol.
