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Franklin R W Van De Goot - One of the best experts on this subject based on the ideXlab platform.
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a new method to determine Wound age in early vital skin injuries a probability scoring system using expression levels of fibronectin cd62p and factor viii in Wound Hemorrhage
Forensic Science International, 2014Co-Authors: Franklin R W Van De Goot, Ibrahim H Korkmaz, Judith Fronczek, Birgit I Witte, Rob Visser, Magda M W Ulrich, Mark P V Begieneman, Lawrence RozendaalAbstract:PURPOSE OF THE STUDY: In forensic autopsies it is important to determine the age of early vital skin Wounds as accurate as possible. In addition to inflammation, coagulation is also induced in vital Wounds. Analysis of blood coagulation markers in Wound Hemorrhage could therefore be an important additional discriminating factor in Wound age determination. The aim of this study was to develop a Wound age probability scoring system, based on the immunohistochemical expression levels of Fibronectin, CD62p and Factor VIII in Wound Hemorrhage. METHODS: Tissue samples of (A) non injured control skin (n=383), and samples of mechanically induced skin injuries of known Wound age, (B) injuries inflicted shortly before death (up to a few minutes old) (n=382), and (C) injuries inflicted 15-30 min before death (n=42) were obtained at autopsy in order to validate Wound age estimation. Tissue slides were stained for Fibronectin, CD62p and Factor VIII and were subsequently scored for staining intensity (IHC score) in Wound Hemorrhage (1=minor, 2=moderate, 3=strong positive). Finally, probability scores of these markers were calculated. RESULTS: In at most 14% of the non-injured control samples, Hemorrhage was found, with mean±standard deviation IHC scores of 0.1±0.4, 0.2±0.4 and 0.2±0.5 for Fibronectin, CD62p, and Factor VIII, respectively. Expression of these markers significantly increased to mean IHC scores 1.4±0.8 (Fibronectin), 1.2±0.6 (CD62p), and 1.6±0.8 (Factor VIII) in Wounds inflicted shortly before death (few minutes old) and to 2.6±0.5 (Fibronectin), 2.5±0.6 (CD62p), and 2.8±0.4 (Factor VIII) in 15-30 min old Wounds. The probabilities that a Wound was non vital in case of an IH score 0 were 87%, 88% and 90% for Fibronectin, CD62p, and Factor VIII, respectively. In case of an IHC score 1 or 2, the probabilities that a Wound was a few minutes old were 82/90%, 82/83% and 72/93%. Finally, in case of an IHC score 3, the probabilities that a Wound was 15-30 min old were 65%, 76% and 55%. CONCLUSIONS: Based on the expression of Fibronectin, CD62p and Factor VIII in Wound Hemorrhage, we developed a probability scoring system that can be used in forensic autopsies to improve Wound age estimation in early skin injuries.
Lawrence Rozendaal - One of the best experts on this subject based on the ideXlab platform.
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a new method to determine Wound age in early vital skin injuries a probability scoring system using expression levels of fibronectin cd62p and factor viii in Wound Hemorrhage
Forensic Science International, 2014Co-Authors: Franklin R W Van De Goot, Ibrahim H Korkmaz, Judith Fronczek, Birgit I Witte, Rob Visser, Magda M W Ulrich, Mark P V Begieneman, Lawrence RozendaalAbstract:PURPOSE OF THE STUDY: In forensic autopsies it is important to determine the age of early vital skin Wounds as accurate as possible. In addition to inflammation, coagulation is also induced in vital Wounds. Analysis of blood coagulation markers in Wound Hemorrhage could therefore be an important additional discriminating factor in Wound age determination. The aim of this study was to develop a Wound age probability scoring system, based on the immunohistochemical expression levels of Fibronectin, CD62p and Factor VIII in Wound Hemorrhage. METHODS: Tissue samples of (A) non injured control skin (n=383), and samples of mechanically induced skin injuries of known Wound age, (B) injuries inflicted shortly before death (up to a few minutes old) (n=382), and (C) injuries inflicted 15-30 min before death (n=42) were obtained at autopsy in order to validate Wound age estimation. Tissue slides were stained for Fibronectin, CD62p and Factor VIII and were subsequently scored for staining intensity (IHC score) in Wound Hemorrhage (1=minor, 2=moderate, 3=strong positive). Finally, probability scores of these markers were calculated. RESULTS: In at most 14% of the non-injured control samples, Hemorrhage was found, with mean±standard deviation IHC scores of 0.1±0.4, 0.2±0.4 and 0.2±0.5 for Fibronectin, CD62p, and Factor VIII, respectively. Expression of these markers significantly increased to mean IHC scores 1.4±0.8 (Fibronectin), 1.2±0.6 (CD62p), and 1.6±0.8 (Factor VIII) in Wounds inflicted shortly before death (few minutes old) and to 2.6±0.5 (Fibronectin), 2.5±0.6 (CD62p), and 2.8±0.4 (Factor VIII) in 15-30 min old Wounds. The probabilities that a Wound was non vital in case of an IH score 0 were 87%, 88% and 90% for Fibronectin, CD62p, and Factor VIII, respectively. In case of an IHC score 1 or 2, the probabilities that a Wound was a few minutes old were 82/90%, 82/83% and 72/93%. Finally, in case of an IHC score 3, the probabilities that a Wound was 15-30 min old were 65%, 76% and 55%. CONCLUSIONS: Based on the expression of Fibronectin, CD62p and Factor VIII in Wound Hemorrhage, we developed a probability scoring system that can be used in forensic autopsies to improve Wound age estimation in early skin injuries.
Mark P V Begieneman - One of the best experts on this subject based on the ideXlab platform.
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a new method to determine Wound age in early vital skin injuries a probability scoring system using expression levels of fibronectin cd62p and factor viii in Wound Hemorrhage
Forensic Science International, 2014Co-Authors: Franklin R W Van De Goot, Ibrahim H Korkmaz, Judith Fronczek, Birgit I Witte, Rob Visser, Magda M W Ulrich, Mark P V Begieneman, Lawrence RozendaalAbstract:PURPOSE OF THE STUDY: In forensic autopsies it is important to determine the age of early vital skin Wounds as accurate as possible. In addition to inflammation, coagulation is also induced in vital Wounds. Analysis of blood coagulation markers in Wound Hemorrhage could therefore be an important additional discriminating factor in Wound age determination. The aim of this study was to develop a Wound age probability scoring system, based on the immunohistochemical expression levels of Fibronectin, CD62p and Factor VIII in Wound Hemorrhage. METHODS: Tissue samples of (A) non injured control skin (n=383), and samples of mechanically induced skin injuries of known Wound age, (B) injuries inflicted shortly before death (up to a few minutes old) (n=382), and (C) injuries inflicted 15-30 min before death (n=42) were obtained at autopsy in order to validate Wound age estimation. Tissue slides were stained for Fibronectin, CD62p and Factor VIII and were subsequently scored for staining intensity (IHC score) in Wound Hemorrhage (1=minor, 2=moderate, 3=strong positive). Finally, probability scores of these markers were calculated. RESULTS: In at most 14% of the non-injured control samples, Hemorrhage was found, with mean±standard deviation IHC scores of 0.1±0.4, 0.2±0.4 and 0.2±0.5 for Fibronectin, CD62p, and Factor VIII, respectively. Expression of these markers significantly increased to mean IHC scores 1.4±0.8 (Fibronectin), 1.2±0.6 (CD62p), and 1.6±0.8 (Factor VIII) in Wounds inflicted shortly before death (few minutes old) and to 2.6±0.5 (Fibronectin), 2.5±0.6 (CD62p), and 2.8±0.4 (Factor VIII) in 15-30 min old Wounds. The probabilities that a Wound was non vital in case of an IH score 0 were 87%, 88% and 90% for Fibronectin, CD62p, and Factor VIII, respectively. In case of an IHC score 1 or 2, the probabilities that a Wound was a few minutes old were 82/90%, 82/83% and 72/93%. Finally, in case of an IHC score 3, the probabilities that a Wound was 15-30 min old were 65%, 76% and 55%. CONCLUSIONS: Based on the expression of Fibronectin, CD62p and Factor VIII in Wound Hemorrhage, we developed a probability scoring system that can be used in forensic autopsies to improve Wound age estimation in early skin injuries.
Marchal Guy - One of the best experts on this subject based on the ideXlab platform.
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Transcatheter embolization of iatrogenic postoperative Wound Hemorrhage in the left hip
Churchill livingstone, 1999Co-Authors: Maleux Geert, Stuyck J, Stockx L, Wilms Guy, Marchal GuyAbstract:A 48-year-old man underwent resection of a Moore prosthesis in his left hip because of septic arthritis. The patient developed a major postoperative Wound hematoma in the left hip and significant drop in hemoglobin, which needed blood transfusion. Angiography was performed to locate and to treat the bleeding. Superselective catheterization, followed by embolization, using microparticles, produced total occlusion of the injured vessel. There was immediate cessation of bleeding and the patient recovered completely. (C) 1999 Harcourt Publishers Ltd.status: publishe
Ibrahim H Korkmaz - One of the best experts on this subject based on the ideXlab platform.
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a new method to determine Wound age in early vital skin injuries a probability scoring system using expression levels of fibronectin cd62p and factor viii in Wound Hemorrhage
Forensic Science International, 2014Co-Authors: Franklin R W Van De Goot, Ibrahim H Korkmaz, Judith Fronczek, Birgit I Witte, Rob Visser, Magda M W Ulrich, Mark P V Begieneman, Lawrence RozendaalAbstract:PURPOSE OF THE STUDY: In forensic autopsies it is important to determine the age of early vital skin Wounds as accurate as possible. In addition to inflammation, coagulation is also induced in vital Wounds. Analysis of blood coagulation markers in Wound Hemorrhage could therefore be an important additional discriminating factor in Wound age determination. The aim of this study was to develop a Wound age probability scoring system, based on the immunohistochemical expression levels of Fibronectin, CD62p and Factor VIII in Wound Hemorrhage. METHODS: Tissue samples of (A) non injured control skin (n=383), and samples of mechanically induced skin injuries of known Wound age, (B) injuries inflicted shortly before death (up to a few minutes old) (n=382), and (C) injuries inflicted 15-30 min before death (n=42) were obtained at autopsy in order to validate Wound age estimation. Tissue slides were stained for Fibronectin, CD62p and Factor VIII and were subsequently scored for staining intensity (IHC score) in Wound Hemorrhage (1=minor, 2=moderate, 3=strong positive). Finally, probability scores of these markers were calculated. RESULTS: In at most 14% of the non-injured control samples, Hemorrhage was found, with mean±standard deviation IHC scores of 0.1±0.4, 0.2±0.4 and 0.2±0.5 for Fibronectin, CD62p, and Factor VIII, respectively. Expression of these markers significantly increased to mean IHC scores 1.4±0.8 (Fibronectin), 1.2±0.6 (CD62p), and 1.6±0.8 (Factor VIII) in Wounds inflicted shortly before death (few minutes old) and to 2.6±0.5 (Fibronectin), 2.5±0.6 (CD62p), and 2.8±0.4 (Factor VIII) in 15-30 min old Wounds. The probabilities that a Wound was non vital in case of an IH score 0 were 87%, 88% and 90% for Fibronectin, CD62p, and Factor VIII, respectively. In case of an IHC score 1 or 2, the probabilities that a Wound was a few minutes old were 82/90%, 82/83% and 72/93%. Finally, in case of an IHC score 3, the probabilities that a Wound was 15-30 min old were 65%, 76% and 55%. CONCLUSIONS: Based on the expression of Fibronectin, CD62p and Factor VIII in Wound Hemorrhage, we developed a probability scoring system that can be used in forensic autopsies to improve Wound age estimation in early skin injuries.
