The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Subhash L Bodhankar - One of the best experts on this subject based on the ideXlab platform.
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naringin a flavanone glycoside promotes angiogenesis and inhibits endothelial apoptosis through modulation of inflammatory and growth factor expression in diabetic foot ulcer in rats
Chemico-Biological Interactions, 2014Co-Authors: Amit D Kandhare, Pinaki Ghosh, Subhash L BodhankarAbstract:Chronic, unhealed diabetic foot ulcer (DFU) is one of the most severe complications of diabetes mellitus (DM). Naringin, a flavanone glycoside antioxidant, was reported to have antidiabetic and anti-apoptotic properties. In the present study DM was induced experimentally by streptozotocin (STZ, 55 mg/kg, i.p.). In surgically introduced Wounds on the dorsal surface of the hind paw of rats, the healing potential of naringin was investigated. Rats were treated with naringin (20, 40 and 80 mg/kg, p.o.), insulin (10 IU/kg, s.c.) and tetrachlorodecaoxide (TCDO) (1 drop, twice a day, topically) for 16 days. The Wound area was measured every second day, and on day 17 various biochemical parameters were determined in serum, Wound Tissue, and histopathological examination of the Wound was performed. Naringin (40 and 80 mg/kg) significantly (P<0.05) improved Wound area, serum glucose level, glycated Hb and serum insulin. Naringin treatment at 40 and 80 mg/kg resulted in significant (P<0.05) up-regulation of mRNA expression of growth factor (IFG-1, TGF-β and VEGF-c), Ang-1 and collagen-1 whereas mRNA expression of inflammatory mediators (TNF-α, IL-1β and IL-6) was down-regulated. Furthermore, naringin significantly (P<0.05) attenuated STZ-induced apoptosis and stimulated angiogenesis in the Wound Tissue. Further results suggest that angiogenesis was improved via naringin-mediated inhibition of hyperglycemia, oxidative stress, down-regulation of inflammatory mediator expression and up-regulation of growth factor expression, leading to improved Wound healing of DFU.
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naringin a flavanone glycoside promotes angiogenesis and inhibits endothelial apoptosis through modulation of inflammatory and growth factor expression in diabetic foot ulcer in rats
Chemico-Biological Interactions, 2014Co-Authors: Amit D Kandhare, Pinaki Ghosh, Subhash L BodhankarAbstract:Abstract Chronic, unhealed diabetic foot ulcer (DFU) is one of the most severe complications of diabetes mellitus (DM). Naringin, a flavanone glycoside antioxidant, was reported to have antidiabetic and anti-apoptotic properties. In the present study DM was induced experimentally by streptozotocin (STZ, 55 mg/kg, i.p.). In surgically introduced Wounds on the dorsal surface of the hind paw of rats, the healing potential of naringin was investigated. Rats were treated with naringin (20, 40 and 80 mg/kg, p.o.), insulin (10 IU/kg, s.c.) and tetrachlorodecaoxide (TCDO) (1 drop, twice a day, topically) for 16 days. The Wound area was measured every second day, and on day 17 various biochemical parameters were determined in serum, Wound Tissue, and histopathological examination of the Wound was performed. Naringin (40 and 80 mg/kg) significantly (P
Sashwati Roy - One of the best experts on this subject based on the ideXlab platform.
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direct conversion of injury site myeloid cells to fibroblast like cells of granulation Tissue
Nature Communications, 2018Co-Authors: Mithu Sinha, Kanhaiya Singh, Amitava Das, Subhadip Ghatak, Ia Rhea, Itani N Lackstone, Heathe M Powell, Savita Khanna, Sashwati RoyAbstract:Inflammation, following injury, induces cellular plasticity as an inherent component of physiological Tissue repair. The dominant fate of Wound macrophages is unclear and debated. Here we show that two-thirds of all granulation Tissue fibroblasts, otherwise known to be of mesenchymal origin, are derived from myeloid cells which are likely to be Wound macrophages. Conversion of myeloid to fibroblast-like cells is impaired in diabetic Wounds. In cross-talk between keratinocytes and myeloid cells, miR-21 packaged in extracellular vesicles (EV) is required for cell conversion. EV from Wound fluid of healing chronic Wound patients is rich in miR-21 and causes cell conversion more effectively compared to that by fluid from non-healing patients. Impaired conversion in diabetic Wound Tissue is rescued by targeted nanoparticle-based delivery of miR-21 to macrophages. This work introduces a paradigm wherein myeloid cells are recognized as a major source of fibroblast-like cells in the granulation Tissue.
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oxygenation state as a driver of myofibroblast differentiation and Wound contraction hypoxia impairs Wound closure
Journal of Investigative Dermatology, 2010Co-Authors: Chandan K Sen, Sashwati RoyAbstract:Myofibroblasts are ubiquitous in the human body and may form from the differentiation of fibroblasts, epithelial cells, endothelial cells, and mononuclear cells, among others. Their clinical significance could be substantial, depending on biomedical context. Myofibroblasts help contract open skin Wounds, but they could also be key drivers of fibrosis across numerous Tissue systems and support tumor invasiveness. Understanding the molecular events underlying myofibroblast formation is significant for many human diseases. In this issue, Modarressi et al. address the significance of Wound Tissue hypoxia in impairing Wound contraction by compromising myofibroblast formation. They present compelling evidence indicating Tissue hypoxia conflicts with Wound closure. We are reminded that correcting Wound Tissue hypoxia is critical for the Tissue's response to other therapeutic interventions.
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dermal excisional Wound healing in pigs following treatment with topically applied pure oxygen
Mutation Research, 2005Co-Authors: Richard B Fries, Sashwati Roy, Gayle M Gordillo, William A Wallace, Periannan Kuppusamy, Valerie K Bergdall, Scott W Melvin, Chandan K SenAbstract:Hypoxia, caused by disrupted vasculature and peripheral vasculopathies, is a key factor that limits dermal Wound healing. Factors that can increase oxygen delivery to the regional Tissue, such as supplemental oxygen, warmth, and sympathetic blockade, can accelerate healing. Clinical experience with adjunctive hyperbaric oxygen therapy (HBOT) in the treatment of chronic Wounds have shown that Wound hyperoxia may increase granulation Tissue formation and accelerate Wound contraction and secondary closure. However, HBOT is not applicable to all Wound patients and may pose the risk of oxygen toxicity. Thus, the efficacy of topical oxygen treatment in an experimental setting using the pre-clinical model involving excisional dermal Wound in pigs was assessed. Exposure of open dermal Wounds to topical oxygen treatment increased Tissue pO2 of superficial Wound Tissue. Repeated treatment accelerated Wound closure. Histological studies revealed that the Wounds benefited from the treatment. The oxygen treated Wounds showed signs of improved angiogenesis and Tissue oxygenation. Topically applied pure oxygen has the potential of benefiting some Wound types. Further studies testing the potential of topical oxygen in pre-clinical and clinical settings are warranted.
Amitava Das - One of the best experts on this subject based on the ideXlab platform.
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direct conversion of injury site myeloid cells to fibroblast like cells of granulation Tissue
Nature Communications, 2018Co-Authors: Mithu Sinha, Kanhaiya Singh, Amitava Das, Subhadip Ghatak, Ia Rhea, Itani N Lackstone, Heathe M Powell, Savita Khanna, Sashwati RoyAbstract:Inflammation, following injury, induces cellular plasticity as an inherent component of physiological Tissue repair. The dominant fate of Wound macrophages is unclear and debated. Here we show that two-thirds of all granulation Tissue fibroblasts, otherwise known to be of mesenchymal origin, are derived from myeloid cells which are likely to be Wound macrophages. Conversion of myeloid to fibroblast-like cells is impaired in diabetic Wounds. In cross-talk between keratinocytes and myeloid cells, miR-21 packaged in extracellular vesicles (EV) is required for cell conversion. EV from Wound fluid of healing chronic Wound patients is rich in miR-21 and causes cell conversion more effectively compared to that by fluid from non-healing patients. Impaired conversion in diabetic Wound Tissue is rescued by targeted nanoparticle-based delivery of miR-21 to macrophages. This work introduces a paradigm wherein myeloid cells are recognized as a major source of fibroblast-like cells in the granulation Tissue.
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low oxidative stress mediated proliferation via jnk foxo3a catalase signaling in transplanted adult stem cells promotes Wound Tissue regeneration
Antioxidants & Redox Signaling, 2017Co-Authors: Amitava Das, Neha R Dhoke, Ramasatyaveni GeesalaAbstract:Abstract Aims: Stem cells exposed to pathological levels of reactive oxygen species (ROS) at Wound sites fail to regenerate Tissue. The molecular mechanism underlying differential levels of ROS-mediated regulation of stem cells remains elusive. This study elucidates the mechanistic role of catalase at 10 μM H2O2-induced proliferation of mouse bone marrow stromal (BMSC) and hematopoietic (HSPC) stem/progenitor cells. Results: BMSCs and HSPCs depicted an increased growth rate and colony formation, in the presence of 10 μM but not 100 μM concentration of H2O2, an effect that was perturbed by Vit. C. Mechanistically, JNK activation–FOXO3a nuclear translocation and binding of FOXO3a to catalase promoter at 10 μM H2O2 led to an increased expression and activity of anti-oxidant gene, catalase. This was followed by an increased proliferative phenotype via the AKT-dependent pathway that was perturbed in the presence of catalase-inhibitor, 3-aminotriazole due to an increased ROS-mediated inactivation of AKT. Precli...
Elof Eriksson - One of the best experts on this subject based on the ideXlab platform.
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topically delivered minocycline penetrates a full thickness burn eschar and reduces Tissue bacterial counts
Journal of Burn Care & Research, 2018Co-Authors: Lu Yang, Michael Broomhead, Kristo Nuutila, Karl Proppe, Elof ErikssonAbstract:Injuries to the skin are often complicated by invasive infections. Standard treatment with intravenous antibiotics has limited Tissue penetration and sometimes, major systemic toxicity. Traditional topical delivery of antimicrobials also has limited effectiveness and duration of action. We demonstrate the use of a new Platform Wound Device (PWD) for delivery of topical, ultrahigh concentrations of minocycline as well as lidocaine onto the burn eschar and on the surface of excisional Wounds in a total of 56 burn Wounds and 24 excisional Wounds in a porcine model. Wounds were created on day 0, debrided on day 3, and pigs were killed on day 7. After 3 days of PWD with minocycline treatment, bacterial count was 5.44 log CFU/g in dorsal Wound Tissue inoculated with methicillin-resistant Staphylococcus aureus, less than that after treatment with silver sulfadiazine cream (7.64 log CFU/g). Pain was also relieved or eliminated in burn Wounds and full-thickness excisional Wounds when lidocaine was delivered by the PWD. The results demonstrate that ultrahigh concentrations of antibiotics can be delivered effectively by the PWD, and will accelerate Wound bed preparation.
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Wound fluid bacterial levels exceed Tissue bacterial counts in controlled porcine partial thickness burn infections
Plastic and Reconstructive Surgery, 2003Co-Authors: Karl H Breuing, Paul Y. Liu, Stuart Kaplan, Andrew B Onderdonk, Elof ErikssonAbstract:In the present study, an established controlled burn Wound model was used to test the hypothesis that controlled surface contamination with Staphylococcus aureus is capable of generating a noninvasive method for the creation of a reproducible deep Tissue burn Wound infection. Using a liquid tight-Wound chamber in Yorkshire pigs, partial-thickness burns were inoculated with saline-immersed S. aureus for 24 hours. Noninoculated burns and unWounded skin immersed in normal saline served as controls. Bacterial cultures of Wound fluid were performed daily, and Tissue biopsies for bacteriological and histological evaluations were performed on days 1, 3, and 5. S. aureus was only recovered from S. aureus-inoculated Wounds (Tissue and fluid), whereas all controls contained endogenous Staphylococcus epidermidis only. The number of colony-forming units per gram of Wound Tissue did not correlate with the bacterial counts found in the overlying Wound fluid for any Wounds. Fluid counts were consistently higher than Tissue counts by two logs. S. aureus-inoculated Wounds showed three times deeper Tissue destruction than control Wounds. Obtaining consistently deep Tissue colonization without cross-contamination among Wounds, this study introduces a noninvasive model for controlled burn Wound infection suitable for future investigations regarding the efficacy of topical antibiotic Wound treatment in experimental burns. (Plast. Reconstr. Surg. 111: 781, 2003.)
Mohamed Baguneid - One of the best experts on this subject based on the ideXlab platform.
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a microbiome and metabolomic signature of phases of cutaneous healing identified by profiling sequential acute Wounds of human skin an exploratory study
PLOS ONE, 2020Co-Authors: Mohammed Ashrafi, Yun Xu, Howbeer Muhamadali, Iain White, Maxim Wilkinson, Katherine A Hollywood, Mohamed BaguneidAbstract:Profiling skin microbiome and metabolome has been utilised to gain further insight into Wound healing processes. The aims of this multi-part temporal study in 11 volunteers were to analytically profile the dynamic Wound Tissue and headspace metabolome and sequence microbial communities in acute Wound healing at days 0, 7, 14, 21 and 28, and to investigate their relationship to Wound healing, using non-invasive quantitative devices. Metabolites were obtained using Tissue extraction, sorbent and polydimethylsiloxane patches and analysed using GCMS. PCA of Wound Tissue metabolome clearly separated time points with 10 metabolites of 346 being involved in separation. Analysis of variance-simultaneous component analysis identified a statistical difference between the Wound headspace metabolome, sites (P = 0.0024) and time points (P<0.0001), with 10 out of the 129 metabolites measured involved with this separation between sites and time points. A reciprocal relationship between Staphylococcus spp. and Propionibacterium spp. was observed at day 21 (P<0.05) with a statistical correlation between collagen and Propionibacterium (r = 0.417; P = 0.038) and Staphylococcus (r = -0.434; P = 0.03). Procrustes analysis showed a statistically significant similarity between Wound headspace and Tissue metabolome with non-invasive Wound devices. This exploratory study demonstrates the temporal and dynamic nature of acute Wound metabolome and microbiome presenting a novel class of biomarkers that correspond to Wound healing, with further confirmatory studies now necessary.
