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Carlos A. Rosa - One of the best experts on this subject based on the ideXlab platform.

  • Characterisation of the diversity and physiology of cellobiose-fermenting yeasts isolated from rotting wood in Brazilian ecosystems.
    Fungal biology, 2018
    Co-Authors: Mariana R. Lopes, Paula B. Morais, Carla A. Lara, Marina E.f. Moura, Ana Paula Trovatti Uetanabaro, Marcos José Salgado Vital, Carlos A. Rosa
    Abstract:

    Abstract We investigated the yeast species associated with rotting wood samples obtained from Brazilian ecosystems, with a special focus on cellobiose-fermenting species. About 647 yeast strains were isolated from rotting wood samples collected from the areas of Atlantic rainforest, Cerrado, and Amazonian forest. Eighty-six known species and 47 novel species of yeasts were isolated. Candida boidinii, Cyberlindnera subsufficiens, Meyerozyma guilliermondii, Schwanniomyces polymorphus, Candida natalensis, and Debaryomyces hansenii were the most frequently isolated species. Among the cellobiose-fermenting yeasts, 14 known and three novel yeast species were identified. Scheffersomyces queiroziae, Sc. amazonensis, Yamadazyma sp.1, Hanseniaspora opuntiae, C. jaroonii, and Candida tammaniensis were the main ethanol-producing yeasts. These species also produced an intracellular β-glucosidase responsible for cellobiose hydrolysis. In fermentation assays using a culture medium containing 50 g L−1 cellobiose, ethanol production was observed in all cases; Sc. queiroziae and Sc. amazonensis showed the highest yield, efficiency, and productivity. Candida jaroonii and Yamadazyma sp.1 strains also showed high efficiency in cellobiose fermentation, while C. tammaniensis and H. opuntiae strains produced low amounts of ethanol. This study shows the potential of rotting wood samples from Brazilian ecosystems as a source of yeasts, including new species as well as those with promising biotechnological properties.

  • Yamadazyma barbieri f.a. sp. nov., an ascomycetous anamorphic yeast isolated from a Mid-Atlantic Ridge hydrothermal site (-2300 m) and marine coastal waters.
    International journal of systematic and evolutionary microbiology, 2016
    Co-Authors: Gaëtan Burgaud, Monika Coton, Noémie Jacques, Stella Debaets, Natália O. P. Maciel, Carlos A. Rosa, Mário Gadanho, José Paulo Sampaio, Serge Casaregola
    Abstract:

    Two yeast strains that are members of the same species were isolated from different marine habitats, i.e. one from Mid-Atlantic Ridge ocean water samples located in the direct vicinity of black smokers near the Rainbow deep-sea hydrothermal vent and one from Brazilian marine water samples off the Ipanema beach. Strains CLIB 1964T and CLIB 1965 are anamorphic ascomycetous yeasts affiliated to the Yamadazyma clade of Saccharomycetales. Interestingly, these strains were phylogenetically and distinctly positioned into a group of species comprising all species of the genus Yamadazyma isolated from marine habitats including deep-sea hydrothermal vents, i.e.Candida atmosphaerica,C. spencermartinsiae,C. atlantica,C. oceani and C. taylorii. These strains differed significantly in their D1/D2 domain sequences of the LSU rRNA gene from the closely related species mentioned above, by 2.6, 3.0, 3.4, 3.8 and 6.0 %, respectively. Internal transcribed spacer region sequence divergence was also significant and corresponded to 4.6, 4.7, 4.7, 12.0 and 24.7 % with C. atlantica,C. atmosphaerica, C. spencermartinsiae,C. oceani and C. taylorii, respectively. Phenotypically, strains CLIB 1964T and CLIB 1965 could be distinguished from closely related species by their inability to assimilate l-sorbose. CLIB 1964T (=CBS 14301T=UBOCC-A-214001T) is the designated type strain for Yamadazyma barbieri sp. nov. The MycoBank number is MB 815884.

  • Yamadazyma barbieri f.a. sp nov., an ascomycetous anamorphic yeast isolated from a Mid-Atlantic Ridge hydrothermal site (-2300m) and marine coastal waters
    International Journal of Systematic and Evolutionary Microbiology, 2016
    Co-Authors: Gaëtan Burgaud, Monika Coton, Stella Debaets, Natália O. P. Maciel, Carlos A. Rosa, Mário Gadanho, José Paulo Sampaio, Noémie Vignolles, Serge Casaregola
    Abstract:

    Two yeast strains that are members of the same species were isolated from different marine habitats, i.e. one from Mid-Atlantic Ridge ocean water samples located in the direct vicinity of black smokers near the Rainbow deep-sea hydrothermal vent and one from Brazilian marine water samples off the Ipanema beach. Strains CLIB 1964(T) and CLIB 1965 are anamorphic ascomycetous yeasts affiliated to the Yamadazyma clade of Saccharomycetales. Interestingly, these strains were phylogenetically and distinctly positioned into a group of species comprising all species of the genus Yamadazyma isolated from marine habitats including deep-sea hydrothermal vents, i.e. Candida atmosphaerica, C. spencermartinsiae, C. atlantica, C. oceani and C. taylorii. These strains differed significantly in their D1/D2 domain sequences of the LSU rRNA gene from the closely related species mentioned above, by 2.6, 3.0, 3.4, 3.8 and 6.0 %, respectively. Internal transcribed spacer region sequence divergence was also significant and corresponded to 4.6, 4.7, 4.7, 12.0 and 24.7% with C. atlantica, C. atmosphaerica, C. spencermartinsiae, C. oceani and C. taylorii, respectively. Phenotypically, strains CLIB 1964(T) and CLIB 1965 could be distinguished from closely related species by their inability to assimilate L-sorbose. CLIB 1964(T) (= CBS 14301(T) = UBOCC-A-214001(T)) is the designated type strain for Yamadazyma barbieri sp. nov. The MycoBank number is MB 815884.

  • Xylitol production by yeasts isolated from rotting wood in the Galápagos Islands, Ecuador, and description of Cyberlindnera galapagoensis f.a., sp. nov.
    Antonie van Leeuwenhoek, 2015
    Co-Authors: Maria C. Guamán-burneo, Kelly J. Dussán, Raquel M. Cadete, Monaliza A. M. Cheab, Patricia Portero, Enrique J. Carvajal-barriga, Sílvio S. Silva, Carlos A. Rosa
    Abstract:

    This study evaluated d -xylose-assimilating yeasts that are associated with rotting wood from the Galápagos Archipelago, Ecuador, for xylitol production from hemicellulose hydrolysates. A total of 140 yeast strains were isolated. Yeasts related to the clades Yamadazyma, Kazachstania, Kurtzmaniella, Lodderomyces, Metschnikowia and Saturnispora were predominant. In culture assays using sugarcane bagasse hemicellulose hydrolysate, Candida tropicalis CLQCA-24SC-125 showed the highest xylitol production, yield and productivity (27.1 g L^−1 xylitol, Y _p/s ^xyl  = 0.67 g g^−1, Q_p = 0.38 g L^−1. A new species of Cyberlindnera, strain CLQCA-24SC-025, was responsible for the second highest xylitol production (24 g L^−1, Y _p/s ^xyl  = 0.64 g g^−1, Q_p = 0.33 g L^−1 h^−1) on sugarcane hydrolysate. The new xylitol-producing species Cyberlindnera galapagoensis f.a., sp. nov., is proposed to accommodate the strain CLQCA-24SC-025^T (=UFMG-CM-Y517^T; CBS 13997^T). The MycoBank number is MB 812171.

  • Yamadazyma riverae sp. nov., a yeast species isolated from plant materials
    International journal of systematic and evolutionary microbiology, 2015
    Co-Authors: Mariana R. Lopes, Mariana C. Ferreira, Tatiana F. C. Carvalho, Fernando C. Pagnocca, Rafaella A. Chagas, Paula B. Morais, Luiz H. Rosa, Marc-andré Lachance, Carlos A. Rosa
    Abstract:

    Nine strains of a novel yeast species were isolated from rotting wood, tree bark, ant nests or living as endophytes in leaves of Vellozia gigantea. Analysis of the sequences of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit rRNA gene showed that this species was related to Candida insectorum in the Yamadazyma clade. The novel species differed from closely related species by 10 and 11 substitutions in the ITS region and the D1/D2 domains of the large subunit of the rRNA gene, respectively. The species is heterothallic and forms asci with one to two hat-shaped ascospores. The name Yamadazyma riverae sp. nov. is proposed for the novel species. The type strain is UFMG-CM-Y444T ( = CBS 14121T) and the allotype strain is TT12 ( = CBS 14098 = UFMG-CM-Y577). The Mycobank number is MB 813221.

Gino Ciafardini - One of the best experts on this subject based on the ideXlab platform.

  • Differential Microbial Composition of Monovarietal and Blended Extra Virgin Olive Oils Determines Oil Quality During Storage.
    Microorganisms, 2020
    Co-Authors: B.a. Zullo, Gino Ciafardini
    Abstract:

    Extra virgin olive oil (EVOO) contains a biotic fraction, which is characterized by various microorganisms, including yeasts. The colonization of microorganisms in the freshly produced EVOO is determined by the physicochemical characteristics of the product. The production of blended EVOO with balanced taste, which is obtained by blending several monovarietal EVOOs, modifies the original microbiota of each oil due to the differential physico-chemical characteristics of the blended oil. This study aimed to evaluate the effect of microbial composition on the stability of the quality indices of the monovarietal and blended EVOOs derived from Leccino, Peranzana, Coratina, and Ravece olive varieties after six months of storage. The yeasts survived only in the monovarietal EVOOs during six months of storage. Barnettozyma californica, Candida adriatica, Candida diddensiae, and Yamadazyma terventina were the predominant yeast species, whose abundance varied in the four monovarietal EVOOs. However, the number of yeasts markedly decreased during the first three months of storage in all blended EVOOs. Thus, all blended EVOOs were more stable than the monovarietal EVOOs as the abundance and activity of microorganisms were limited during storage.

  • Evaluation of physiological properties of yeast strains isolated from olive oil and their in vitro probiotic trait
    Food microbiology, 2018
    Co-Authors: B.a. Zullo, Gino Ciafardini
    Abstract:

    Virgin olive oil contains a biotic fraction represented by rich microbiota, including yeasts. The aim of this study was to investigate some physiological properties and the in vitro probiotic potential of yeast strains previously isolated from Italian virgin olive oil. Eleven yeast strains belonging to the species Candida adriatica, Candida diddensiae, Nakazawaea molendini-olei, Nakazawaea wickerhamii, Wickerhamomyces anomalus, and Yamadazyma terventina were used in this study and compared with the reference yeast Saccharomyces boulardii. Present research has demonstrated that unlike Saccharomyces boulardii which produce only satured and monounsatured fatty acids (MUFAs), the olive oil-borne yeast strains also synthesize polyunsatured fatty acids (PUFAs) in quantities greater than those found in olive oil, which provide health benefits. The survival in gastric and pancreatic juices, which is important for probiotic yeasts because it allows them to cross the human intestinal tract, has reached a maximum of 100% when yeast cells were coated with olive oil. Cholesterol was removed by 50% of the studied yeast strains, and among them, the best results were reached by the strains 2032 and 2033 of W. anomalus which appear the best probiotic candidate in terms of the in vitro probiotic trait evaluated. Further experiments are underway to confirm this findings.

  • Effect of lipolytic activity of Candida adriatica, Candida diddensiae and Yamadazyma terventina on the acidity of extra-virgin olive oil with a different polyphenol and water content
    Food microbiology, 2014
    Co-Authors: Gino Ciafardini, B.a. Zullo
    Abstract:

    Abstract Previous microbiological research demonstrated the presence of a rich micro-flora composed mainly of yeasts in the suspended fraction of freshly produced olive oil. Some of the yeasts are considered harmful as they can damage the quality of the olive oil through the hydrolysis of the triacylglycerols. Present research has demonstrated that the lipolytic activity of some lipase-producer strains belonging to a yeast species called Candida adriatica , Candida diddensiae and Yamadazyma terventina can be modulated by the water and the polyphenol content of olive oil. Laboratory tests highlighted a substantial increase in free fatty acid in the inoculated olive oil characterized by high water content and low polyphenol concentration. The acidity of the olive oil samples containing 0.06% and 0.31% of water increased significantly by 33% in the lipase-producer yeast strains tested during a period of 2 weeks of incubation at 30 °C. All other yeasts showed strong lipolytic activity in the presence of 1.31% of water – the only exception to this was the C. adriatica 1985 strain. The phenolic compounds typical of olive oil represent another important factor able to condition the viability and the lipolytic activity of the lipase-producer yeasts. From the tests performed on the olive oil characterized by an increasing content of total polyphenols equal to 84, 150 and 510 mg per kg of oil, the percentage of the lipase-producer yeasts able to hydrolyse the triacylglycerols was respectively 100%, 67% and 11%.

  • Yamadazyma terventina sp. nov., a yeast species of the Yamadazyma clade from Italian olive oils.
    International journal of systematic and evolutionary microbiology, 2012
    Co-Authors: Gino Ciafardini, Biagi Angelo Zullo, Livio Antonielli, Laura Corte, Luca Roscini, Gianluigi Cardinali
    Abstract:

    During an investigation of olive oil microbiota, three yeast strains were found to be divergent from currently classified yeast species according to the sequences of the D1/D2 domain of the gene encoding the rRNA large subunit (LSU) and the internal transcribed spacer region including the gene for 5.8S rRNA. Phylogenetic analysis revealed that these strains, designated CBS 12509, CBS 12510(T) and CBS 12511, represent a novel anascosporogenous species described herein as Yamadazyma terventina sp. nov; the type strain is DAPES 1924(T) (= CBS 12510(T) = NCAIM Y.02028(T)). This novel species was placed in the Yamadazyma clade, with Yamadazyma scolyti, Candida conglobata and Candida aaseri as closest relatives. Y. terventina differs from the above-mentioned species in the ability to strongly assimilate dl-lactate and weakly assimilate ethanol.

Savitree Limtong - One of the best experts on this subject based on the ideXlab platform.

  • Yeast communities of primary and secondary peat swamp forests in southern Thailand
    Antonie van Leeuwenhoek, 2020
    Co-Authors: Chanita Boonmak, Pannida Khunnamwong, Savitree Limtong
    Abstract:

    Khanthuli peat swamp forest (PSF) is one of a few fertile peat swamp forests that remain in Thailand. It is composed of primary PSF and some areas which have been degraded to secondary PSF due to drought, wildfires and land conversion, which have resulted in a decrease in peat layers and change in the species of the plant community. In this study, diversity of yeasts in peat from both primary and secondary PSF areas of the Khanthuli PSF was determined based on culture-dependent approaches, using dilution plate and enrichment techniques. A total of 66 yeast isolates were identified by the analysis of sequence similarity of the D1/D2 region of the large subunit rRNA gene or the combined analysis of sequence of the D1/D2 region and internal transcribed spacer region and confirmed by phylogenetic analysis of the D1/D2 region to belong to 22 known yeast species and six potential new species in the genera Candida ( Kurtzmaniella , Lodderomyces , Ogataea , Pichia and Yamadazyma clades), Clavispora , Cyberlindnera , Galactomyces , Hanseniaspora , Metschnikowia , Saturnispora , Schwanniomyces , Cryptotrichosporon , Pichia , Curvibasidium , Papiliotrema , Rhodotorula , and Saitozyma . The most prevalent yeasts in the primary PSF were Cyberlindnera subsufficiens and Galactomyces candidus , while Saitozyma podzolica was the most frequently found in peat from the secondary PSF. Common yeast species in both, primary and secondary PSF, were Cy. subsufficiens , G. candidus and Rhodotorula mucilaginosa.

  • Yamadazyma endophytica f.a. sp. nov., an ascomycetous yeast species isolated from leaf tissue.
    International journal of systematic and evolutionary microbiology, 2016
    Co-Authors: Pannida Khunnamwong, Savitree Limtong
    Abstract:

    Strain DMKU-CE23T representing a novel yeast species was isolated from tissue of a corn leaf (Zea may L.) collected in Thailand. A phylogenetic analysis based on the combined sequences of the internal transcribed spacer (ITS) region and the D1/D2 region of the LSU rRNA gene indicated that strain DMKU-CE23T belongs to the Yamadazyma clade and is clearly distinct from other related species. It therefore represents a novel species of the genus Yamadazyma although the formation of ascospores was not observed. The strain of novel species was most closely related to the type strain of Yamadazyma epiphylla but with 5.1 % nucleotide substitutions in the ITS region and 3.7 % nucleotide substitutions in the D1/D2 region of the LSU rRNA gene. The name Yamadazyma endophytica f.a., sp. nov. is proposed. The type strain is DMKU-CE23T (=CBS 14163T=TBRC 5174T).

  • The diversity of culturable yeasts in the phylloplane of rice in Thailand
    Annals of Microbiology, 2015
    Co-Authors: Savitree Limtong, Rungluk Kaewwichian
    Abstract:

    One hundred and fifty-six yeast strains were obtained by the enrichment technique from the phylloplanes of 85 rice leaf samples collected from seven provinces in Thailand. On the basis of the D1/D2 domain of the large subunit rRNA gene sequence analysis, 156 strains were identified as 34 known species in 18 genera consisting of 25 species in 13 genera of the phylum Ascomycota and nine species in five genera of the phylum Basidiomycota. The species in the phylum Ascomycota comprised 24 species in 12 genera of the order Saccharomycetales and one species viz . Yarrowia lipolytica in Saccharomycetales incertae sedis . The 24 species viz. Candida glabrata in the Nakaseomyces clade of Saccharomycetaceae, Candida jaroonii , Candida membranifaciens and Candida terebra in the Yamadazyma clade of Debaryomycetaceae, Candida pseudolambica in the Pichia clade of Pichiaceae, Candida ruelliae in the Metschnikowia clade of Metschnikowiaceae, and three unaffiliated clade Candida species ( Candida catenulata , Candida rugosa and Candida tropicalis ); Clavispora lusitaniae , Kodamaea ohmeri , Metschnikowia koreensis and Metschnikowia lopburiensis in Metschnikowiaceae; Cyberlindnera fabianii , Cyberlindnera rhodanensis and Wickerhamomyces ciferrii in Wickerhamomycetaceae; Debaryomyces nepalensis , Meyerozyma caribbica , Meyerozyma guilliermondii , Millerozyma koratensis , and Yamadazyma mexicanum in Debaryomycetaceae; Pichia kudriavzevii in Pichiaceae; and Lachancea thermotolerans in Saccharomycetaceae. The species in Basidiomycota viz. Cryptococcus flavescens , Cryptococcus laurentii , Cryptococcus aff . laurentii and Cryptococcus rajasthanensis in the Tremellales lineage, Bulleromyces clade, Tremellales, Tremellomycetes, Agaricomycotina; Pseudozyma antarctica and Pseudozyma aphidis in Ustilaginales, Ustilaginomycetes, Ustilaginomycotina; Rhodotorula taiwanensis and Sporobolomyces blumeae in Sporidiobolales, Microbotryomycetes, Pucciniomycotina; and Trichosporon asahii in Trichosporonales, Tremellomycetes, Agaricomycotina. The most prevalent species was R. taiwanensis with a 23 % frequency of occurrence followed by Candida tropicalis (16 %) and Cryptococcus fabianii (12 %).

  • Yamadazyma insecticola f.a., sp. nov. and Yamadazyma epiphylla f.a., sp. nov., two novel yeast species
    International Journal of Systematic and Evolutionary Microbiology, 2015
    Co-Authors: Sasitorn Jindamorakot, Rungluk Kaewwichian, Savitree Limtong
    Abstract:

    Two yeast strains representing two novel yeast species were isolated from frass of an unidentified insect (ST-78T) and the external surfaces of rice leaves (YE170T) collected in Thailand. The two strains were genetically, morphologically and phenotypically distinct from recognized species and were found to represent two novel species of the genus Yamadazyma although formation of ascospores was not observed. In terms of pairwise sequence similarity of the D1/D2 region of the large subunit rRNA gene, the closest relative of strain ST-78T was Candida lessepsii CBS 9941T but with 3.8 % nucleotide substitutions, while the closest relative of strain YE170T was strain ST-78T but with 4.3 % nucleotide substitutions. Analysis of the sequences of the internal transcribed spacer 1 and 2 (ITS1–2) regions revealed that strain ST-78 differed from C. lessepsii CBS 9941T by 8.8 % nucleotide substitutions and from strain YE170T by 9.4 % nucleotide substitutions. The result of pairwise sequence similarity of the D1/D2 and ITS1–2 regions together with phylogenetic analysis indicated that strains ST-78T and YE170T represented two novel species within the Yamadazyma clade. The names Yamadazyma insecticola f.a., sp. nov. (type strain ST-78T = BCC 8314T = NBRC 110421T = CBS 13382T; MycoBank no. MB810546) and Yamadazyma epiphylla f.a., sp. nov. (type strain YE170T = BCC 63466T = NBRC 110423T = CBS 13384T; MycoBank no. MB810547) are proposed for the two novel yeast species.

  • Diversity of culturable yeasts in phylloplane of sugarcane in Thailand and their capability to produce indole-3-acetic acid
    World Journal of Microbiology and Biotechnology, 2014
    Co-Authors: Savitree Limtong, Rungluk Kaewwichian, Wichien Yongmanitchai, Hiroko Kawasaki
    Abstract:

    Yeasts were isolated by the enrichment technique from the phylloplane of 94 samples of sugarcane leaf collected from seven provinces in Thailand. All sugarcane leaf samples contained yeasts and 158 yeast strains were obtained. On the basis of the D1/D2 domain of the large subunit rRNA gene sequence analysis, 144 strains were identified to 24 known species in 14 genera belonging to the Ascomycota viz . Candida akabanensis , Candida dendronema , Candida mesorugosa , Candida michaelii , Candida nivariensis , Candida rugosa , Candida orthopsilosis , Candida quercitrusa , Candida tropicalis , Candida xylopsoci , Cyberlindnera fabianii , Cyberlindnera rhodanensis , Debaryomyces nepalensis , Hannaella aff. coprosmaensis , Hanseniaspora guilliermondii , Kluyveromyces marxianus , Lachancea thermotolerans , Lodderomyces elongisporus , Metschnikowia koreensis , Meyerozyma caribbica , Millerozyma koratensis , Pichia kudriavzevii ,  Torulaspora delbrueckii and Wickerhamomyces edaphicus, and 12 species in six genera of the Basidiomycota viz . Cryptococcus flavescens , Cryptococcus laurentii , Cryptococcus rajasthanensis , Kwoniella heveanensis , Rhodosporidium fluviale , Rhodosporidium paludigenum , Rhodotorula mucilaginosa , Rhodotorula sesimbrana , Rhodotorula taiwanensis , Sporidiobolus ruineniae , Sporobolomyces carnicolor and Sporobolomyces nylandii. Seven strains were identical or similar to four undescribed species. Another seven strains represented four novels species in the genus Metschnikowia , Nakazawaea , Wickerhamomyces and Yamadazyma . The results revealed 69 % of the isolated strains were ascomycete yeasts and 31 % were basidiomycete yeast. The most prevalent species was M. caribbica with a 23 % frequency of occurrence followed by Rh. taiwanensis (11 %) and C. tropicalis (10 %). All strains were assessed for indole-3-acetic acid (IAA) producing capability showing that 69 strains had the capability of producing IAA when cultivated in yeast extract peptone dextrose broth supplemented with 1 g/L l -tryptophan. The highest IAA concentration of 565.1 mg/L was produced by R. fluviale DMKU-RK253.

Mariana R. Lopes - One of the best experts on this subject based on the ideXlab platform.

  • Characterisation of the diversity and physiology of cellobiose-fermenting yeasts isolated from rotting wood in Brazilian ecosystems.
    Fungal biology, 2018
    Co-Authors: Mariana R. Lopes, Paula B. Morais, Carla A. Lara, Marina E.f. Moura, Ana Paula Trovatti Uetanabaro, Marcos José Salgado Vital, Carlos A. Rosa
    Abstract:

    Abstract We investigated the yeast species associated with rotting wood samples obtained from Brazilian ecosystems, with a special focus on cellobiose-fermenting species. About 647 yeast strains were isolated from rotting wood samples collected from the areas of Atlantic rainforest, Cerrado, and Amazonian forest. Eighty-six known species and 47 novel species of yeasts were isolated. Candida boidinii, Cyberlindnera subsufficiens, Meyerozyma guilliermondii, Schwanniomyces polymorphus, Candida natalensis, and Debaryomyces hansenii were the most frequently isolated species. Among the cellobiose-fermenting yeasts, 14 known and three novel yeast species were identified. Scheffersomyces queiroziae, Sc. amazonensis, Yamadazyma sp.1, Hanseniaspora opuntiae, C. jaroonii, and Candida tammaniensis were the main ethanol-producing yeasts. These species also produced an intracellular β-glucosidase responsible for cellobiose hydrolysis. In fermentation assays using a culture medium containing 50 g L−1 cellobiose, ethanol production was observed in all cases; Sc. queiroziae and Sc. amazonensis showed the highest yield, efficiency, and productivity. Candida jaroonii and Yamadazyma sp.1 strains also showed high efficiency in cellobiose fermentation, while C. tammaniensis and H. opuntiae strains produced low amounts of ethanol. This study shows the potential of rotting wood samples from Brazilian ecosystems as a source of yeasts, including new species as well as those with promising biotechnological properties.

  • Yamadazyma riverae sp. nov., a yeast species isolated from plant materials
    International journal of systematic and evolutionary microbiology, 2015
    Co-Authors: Mariana R. Lopes, Mariana C. Ferreira, Tatiana F. C. Carvalho, Fernando C. Pagnocca, Rafaella A. Chagas, Paula B. Morais, Luiz H. Rosa, Marc-andré Lachance, Carlos A. Rosa
    Abstract:

    Nine strains of a novel yeast species were isolated from rotting wood, tree bark, ant nests or living as endophytes in leaves of Vellozia gigantea. Analysis of the sequences of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit rRNA gene showed that this species was related to Candida insectorum in the Yamadazyma clade. The novel species differed from closely related species by 10 and 11 substitutions in the ITS region and the D1/D2 domains of the large subunit of the rRNA gene, respectively. The species is heterothallic and forms asci with one to two hat-shaped ascospores. The name Yamadazyma riverae sp. nov. is proposed for the novel species. The type strain is UFMG-CM-Y444T ( = CBS 14121T) and the allotype strain is TT12 ( = CBS 14098 = UFMG-CM-Y577). The Mycobank number is MB 813221.

Rosa, Carlos A. - One of the best experts on this subject based on the ideXlab platform.

  • Yamadazyma riverae sp. nov., a yeast species isolated from plant materials
    International Journal Of Systematic And Evolutionary Microbiology, 2015
    Co-Authors: Lopes, Mariana R., Ferreira, Mariana C., Carvalho, Tatiana F C, Pagnocca, Fernando C., Chagas, Rafaella A., Morais, Paula B., Rosa, Luiz H., Lachance Marc-andré, Rosa, Carlos A.
    Abstract:

    Nine strains of a novel yeast species were isolated from rotting wood, tree bark, ant nests or living as endophytes in leaves of Vellozia gigantea. Analysis of the sequences of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit rRNA gene showed that this species is related to Candida insectorum in the Yamadazyma clade. The new species differs from its closely related species by 10 and 11 substitutions in the ITS region and the D1/D2 domains of the large subunit of the rRNA gene, respectively. The species is heterothallic and forms asci with one to two hat-shaped ascospores. The name Yamadazyma riverae sp. nov. is proposed. The type strain of Yamadazyma riverae sp. nov. is UFMG-CM-Y444T (= CBS 14121) and the allotype strain is TT12 (= CBS 14098 = UFMG-CM-Y577). The Mycobank number is MB 813221